重组人促红素对老年慢性肾衰病人氧自由基及抗氧化酶活性的影响

目的　探讨重组人促红素（ｒ Ｈ  Ｅ Ｐ Ｏ ）对慢性肾衰患者体内氧自由基及抗氧化酶活性的影响。方法　测定 １９ 例老年慢性肾衰尿毒症期患者 ｒ Ｈ  Ｅ Ｐ Ｏ 治疗前后血浆 Ｍ Ｄ Ａ 、 Ｓ Ｏ Ｄ 及红细胞 Ｇ Ｓ Ｈ  Ｐｘ 含量。结果　ｒ Ｈ  Ｅ Ｐ Ｏ 治疗后慢性肾衰病人 Ｍ Ｄ Ａ 含量降低, Ｓ Ｏ Ｄ 、 Ｇ Ｓ Ｈ  Ｐｘ 活性升高。结论　ｒ Ｈ  Ｅ Ｐ Ｏ 纠正肾性贫血同时,可清除体内氧自由基,提高抗氧化酶活性。     

Oxidative stress in systemic lupus erythematosus and rheumatoid arthritis patients: relationship to disease manifestations and activity

Aim: The present work was undertaken to study the status and contribution of oxidative stress in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients. Relationship of the markers of oxidative stress to clinical manifestations, disease activity, damage and medications used were well considered. Methods: Thirty SLE and 30 RA female patients were included in the study and clinical examination and investigations were performed and disease activity was assessed. Markers of oxidative stress, including malondialdehyde (MDA) and antioxidant scavengers with glutathione (GSH) and glutathione peroxidase (GSH Px) were assessed. Results: Level of MDA, GSH and GSH Px were remarkably altered in RA and SLE patients compared to controls. Markers of increased oxidative stress and impaired antioxidant capacity were profound in RA and significantly reflected disease activity in RA and SLE, with special attention to alopecia and lupus nephritis. RA patients receiving methotrexate had significantly altered parameters and the steroid dose in SLE patients correlated with these markers. Conclusion: Oxidative stress was increased and more profound in RA than SLE and could well reflect disease activity, with special attention to SLE patients with alopecia and nephritis. Medications used are closely related to the oxidant/antioxidant imbalance. Considering antioxidants in novel therapeutic strategies is important in SLE and RA patients.     

人参果粗多糖的抗衰老作用研究

目的　观察人参果粗多糖（ＣＰＦＧ）的抗衰老作用。方法　ＣＰＦＧ按２００、４００ ｍ ｇ／ｋｇ 给老年大鼠连续灌胃３０ ｄ,测血清ＭＤＡ含量,脑和肝组织脂褐质（Ｌｆ）含量,皮肤和肝组织羟脯氨酸（Ｈｙｐ）含量及血清ＳＯＤ、ＣＡＴ、ＧＳＨ－Ｐｘ 活性。结果　ＣＰＦＧ 两剂量组均能明显降低老年大鼠血清ＭＤＡ 含量及脑和肝组织Ｌｆ含量,明显提高血清ＳＯＤ、ＣＡＴ、ＧＳＨ－Ｐｘ 活性及皮肤Ｈｙｐ 含量。结论　ＣＰＦＧ可能通过改善自由基代谢发挥抗衰老作用。     

外源性一氧化氮对旋毛虫感染小鼠抗氧化能力的影响

目的探讨外源性NO供体亚硝基铁氰化钠(SNP)对旋毛虫病小鼠肝脏、外周血中抗氧化酶活性及脂质氧化的影响。方法采用消化法分离旋毛虫肌幼虫,感染BALB/c小鼠,400条/只。42 d后,收集感染鼠及正常小鼠外周血、肝脏。设A组(感染鼠肝脏匀浆物+SNP)、B组(正常鼠肝脏匀浆物+SNP)、C组(感染鼠外周血+SNP)、D组(正常鼠外周血+SNP)。每组内设5个SNP反应终浓度,分别为0(空白对照)、2、5、10、30μmol/L,37℃反应30 min,测定各组超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及脂质氧化(MDA)含量。结果与正常鼠相比,旋毛虫感染鼠肝脏、外周血中SOD、CAT、GSH-Px活性上升,MDA含量增加,差异均有统计学意义(P均0.05);在A、B组中,10、30μmol/L SNP作用下SOD、CAT、GSH-Px活性较空白对照降低(P均0.05);与2~30μmol/L SNP反应后,MDA含量较空白对照明显升高(P均0.05)。在C、D组中,30μmol/LSNP作用下SOD、CAT、GSH-Px活性显著下降,MDA浓度升高,与空白对照比较差异均有统计学意义(P均0.01)。在2~30μmol/L浓度区间,SNP浓度与旋毛虫感染鼠肝脏、外周血中SOD、CAT、GSH-Px活性呈负相关(r肝SOD=-0.901,r肝CAT=-0.802,r肝GSH-Px=-0.847,r血SOD=-0.899,r血CAT=-0.685,r血GSH-Px=-0.635,P均0.05),与MDA含量呈正相关(r肝MDA=0.697,r血MDA=0.764,P均0.05)。结论旋毛虫感染可致小鼠肝脏、外周血中活性氧自由基产生增加,SOD、CAT、GSH-Px活性升高;外源性NO可降低旋毛虫感染小鼠SOD、CAT、GSH-Px活性,抑制小鼠的抗氧化能力。     

Increased levels of autoantibodies against catalase and superoxide dismutase associated with oxidative stress in patients with rheumatoid arthritis and systemic lupus erythematosus

OBJECTIVE: To evaluate the level of autoantibodies against superoxide dismutase (SOD) and catalase (CAT) in the sera of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) Tunisian patients, to study the oxidative profile among the same patients and to establish a correlation between the two parameters in order to understand the role of each one in the genesis of the two diseases. METHOD: Using a standard enzyme-linked immunosorbent assay (ELISA), the levels of immunoglobulin G (IgG) and IgM directed against CAT and SOD in the sera of 39 RA patients, 40 SLE patients, and 50 control healthy individuals were evaluated. The oxidative/antioxidative profile was tested by measuring serum malondialdehyde (MDA), conjugated dienes (CD), CAT activity, and SOD activity. RESULTS: Our data showed increased levels of IgG antibodies (Ab) against CAT in both groups of patients (p<0.05) compared to control subjects. However, the SLE patients displayed an increased level of anti-SOD IgG (p<0.05). In all patients the lipid peroxidation was confirmed by high levels of MDA and conjugated dienes (p<0.05). RA patients exhibited an increasing CAT and SOD activity in their sera (p<0.05) with a positive correlation observed between CAT and IgG anti-CAT (p<0.05). The same results were observed for SLE patients. In addition, a positive correlation was observed between anti-CAT Ab and anti-SOD Ab in SLE patients (p<0.05). CONCLUSION: Collectively, these results suggested that the primary factor causing the oxidative stress observed in RA and SLE is excessive free radical production rather than impaired CAT or SOD activity due to autoantibody inhibition.     

Oxidant/antioxidant status in men with Behçet’s disease

Behçet’s disease (BD) is a chronic, progressive disorder that affects many systems of the body including the eye. The aim of this study was to assess whether the increase in oxidative stress in the affected tissues is reflected by lipid peroxidation and to check for alterations in antioxidants and antioxidant enzyme activities in patients with BD. Erythrocyte antioxidant potential (AOP), glutathione (GSH) and GSH-dependent enzymes (glutathione peroxidase (GSH-Px), glutathione reductase (GRD) and glutathione-S-transferase (GST), catalase (CAT), Cu–Zn superoxide dismutase (Cu–Zn SOD) activities, malondialdehyde (MDA) and some trace elements (zinc, Zn; copper, Cu; manganese, Mn) levels in men with BD. Erythrocyte CAT, GSH-Px activities, MDA, GSH, AOP and serum Zn values were significantly lower in patients with BD than in the control group. However, erythrocyte Cu–Zn SOD, GRD activities, erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP) and Cu values were significantly higher in patients with BD than in the control group, but GST activity and serum Mn values were unchanged. In conclusion, our results confirm the presence of oxidative stress in patients with BD and suggest that the severity of BD may arise from impaired antioxidant mechanisms. Therapy with antioxidants may lead to the increase in the antioxidant defense system and thus improvement in clinical symptoms.     

高级氧化蛋白产物在系统性红斑狼疮早发动脉粥样硬化中的临床意义

目的 研究系统性红斑狼疮(SLE)氧化应激状态及早发动脉粥样硬化的情况,并重点探讨高级氧化蛋白产物(AOPPs)在SLE早发动脉粥样硬化中的意义.方法 采用酶联免疫吸附试验(ELISA)法、分光光度法检测44例未绝经女性SLE患者和31名健康对照组血清中AOPPs、同型半胱氨酸(Hey)、丙二醛(MDA)的浓度以及超氧化物歧化酶(SOD)活性,并检测臂踝脉搏波速度(baPWV)以及颈动脉内膜中层厚度(CIMT),进行比较.并进一步将SLE组分为病程≥5年与<5年组、活动组与非活动组,记录SLE患者的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)等指标.采用t检验.t'检验以及Pearson榆验.结果 SLE组AOPPs、Hey、MDA浓度显著高于健康对照组(P<0.05),SOD浓度显著低于健康对照组(P<0.05),并且病程≥5年与<5年组、活动组与非活动组间差异亦有统计学意义(P<0.05);CIMT检查SLE组中有2例存在颈动脉斑块(CAP)(病程均>5年),而31名健康对照组均无CAP形成,且SLE组CIMT、baPWV较对照组明显增高(P<0.05),病程是否≥5年组中比较差异亦有统计学意义(P<0.05);氧化应激指标(AOPPs、Hcy、MDA、SOD)与baPWV、CIMT有显著相关性(P<0.01);AOPPs浓度与Hcy、TC、TG、LDL呈显著正相关(P<0.01～0.05).结论 SLE患者体内存在显著的氧化应激,较对照组有明显的动脉粥样硬化倾向;疾病病程、氧化应激是动脉粥样硬化的重要危险因素;AOPPs与SLE早发动脉粥样硬化相关,可作为一项SLE早发动脉粥样硬化的预测因子.     

Effects of apple consumption on plasma and erythrocyte antioxidant parameters in elderly subjects

The effects of apple consumption on plasma and erythrocyte antioxidant parameters of elderly subjects were investigated in this study. Fifteen elderly subjects (mean age 71.86 +/- 4.17) participated in the study. They consumed an apple a day for 1 month. Before and after this period, fasting blood samples were obtained, and oxidant (malondialdehyde [MDA]) and antioxidant (superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], catalase [CAT], and antioxidant potential [AOP]) parameters were studied. MDA and AOP levels were studied in plasma, and SOD, GSH-Px, and CAT activities and MDA levels were measured in the erythrocytes. In the erythrocytes, GSH-Px and SOD activities were found to be higher (p < .001 and p < .01), but MDA levels were lower in the second samples relative to the first ones. In the plasma, AOP value was found to be higher in the second samples relative to first ones (p < .001). No differences were found, however, between the routine blood parameters such as total cholesterol, low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, high-density-lipoprotein cholesterol, and triglyceride levels. The results show that consumption of apple leads to significant increases in the activities of some antioxidant enzymes and in the antioxidant potential values of the blood, and that decreases oxidation reactions in the body in significant amount. It is quite possible that reduced peroxidation processes owing to consumption of this fruit may play a part in some of their beneficial effects in the elderly subjects.     

Oxidant/Antioxidant Status of the Erythrocytes from Patients with Rheumatoid Arthritis

It has been suggested that enzymatic and/or non-enzymatic antioxidant systems are impaired in rheumatoid arthritis (RA) and hence patients are exposed to oxidant stress. This study aimed to establish whether this is really the case. Fasting blood samples were obtained from 24 patients with rheumatoid arthritis and 20 controls. The activities of erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and xanthine oxidase (XO) enzymes and malondialdehyde (MDA), oxidant resistant (OR) and non-enzymatic superoxide radical scavenger activity (NSSA) values were measured in both groups. Patients with RA had higher SOD and XO activities and MDA levels than did the controls. However, NSSA and OR levels were found to be decreased, and CAT and GSH-Px activities unchanged in the study group. Results suggest that excessive free radical production through the xanthine–xanthine oxidase system is the primary factor in rheumatoid arthritis, rather than an impaired antioxidant system. The therapeutic use of XO enzyme inhibitors and some antioxidants can be beneficial in this regard. Received: 20 May 1999 / Accepted: 27 October 1999     

氟对大鼠脂质过氧化和抗氧化能力的影响

目的 探讨氟对大鼠血和各组织丙二醛（MDA）水平、超氧化物歧化物（SOD）活力及全血谷胱苷肽过氧化物酶（GSH-Px）活力的影响。方法 运用动物实验,采用饮水加氟的方法。结果 染氟可使大鼠血清、肝脏、肾脏、脑中MDA含量显著增加;全血和肝脏、肾、心脏、睾丸的SOD活性显著降低;全血GSH-Px活性降低。结果 氟可促进机体脂质过氧化,抑制抗氧化酶（SOD,GSH-Px）的活力。     

Effects of Apple Consumption on Plasma and Erythrocyte Antioxidant Parameters in Elderly Subjects

The effects of apple consumption on plasma and erythrocyte antioxidant parameters of elderly subjects were investigated in this study. Fifteen elderly subjects (mean age 71.86 ± 4.17) participated in the study. They consumed an apple a day for 1 month. Before and after this period, fasting blood samples were obtained, and oxidant (malondialdehyde [MDA]) and antioxidant (superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], catalase [CAT], and antioxidant potential [AOP]) parameters were studied. MDA and AOP levels were studied in plasma, and SOD, GSH-Px, and CAT activities and MDA levels were measured in the erythrocytes. In the erythrocytes, GSH-Px and SOD activities were found to be higher (p < .001 and p < .01), but MDA levels were lower in the second samples relative to the first ones. In the plasma, AOP value was found to be higher in the second samples relative to first ones (p < .001). No differences were found, however, between the routine blood parameters such as total cholesterol, low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, high-density-lipoprotein cholesterol, and triglyceride levels. The results show that consumption of apple leads to significant increases in the activities of some antioxidant enzymes and in the antioxidant potential values of the blood, and that decreases oxidation reactions in the body in significant amount. It is quite possible that reduced peroxidation processes owing to consumption of this fruit may play a part in some of their beneficial effects in the elderly subjects.     

NO和脂质过氧化与老年矽肺关系的研究

目的　探讨ＮＯ和脂质过氧化与老年矽肺的关系。方法　检测８５ 例老年矽肺患者和８０ 例健康老年人的血浆ＮＯ（Ｐ－ＮＯ）、过氧化脂质（Ｐ－ＬＰＯ）及红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）、过氧化氢酶（Ｅ－ＣＡＴ）、谷胱甘肽过氧化物酶（Ｅ－ＧＳＨ－Ｐｘ）、过氧化脂质（Ｅ－ＬＰＯ）值。结果　与对照组比较,患者组Ｅ－ＳＯＤ、Ｅ－ＣＡＴ、Ｅ－ＧＳＨ－Ｐｘ 均值显著降低,Ｐ－ＮＯ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ 均值显著升高（Ｐ＜ ０．００１）;直线回归和相关分析表明上述各检测值与患者病程均有相关;逐步回归表明患者病情、肺功能状态与Ｐ－ＮＯ、Ｅ－ＳＯＤ、Ｅ－ＬＰＯ 值相关最密切。结论　矽肺患者体内ＮＯ 代谢异常,氧化抗氧化平衡严重失调,氧化和脂质过氧化反应病理性加剧。     

D-半乳糖催老大鼠氧化水平和抗氧化能力的变化

目的　研究人工催老大鼠氧化水平和抗氧化能力的改变。方法　采用D 半乳糖 6 0mg/kg皮下注射 4 2d ,复制人工催老大鼠模型 ,以观察催老大鼠血中丙二醛 (MDA)、一氧化氮 (NO)的含量、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)、一氧化氮合酶 (NOS)和脑单胺氧化酶 (MAO)等活性的变化。结果　催老大鼠与正常大鼠相比 ,血清中MDA含量增高 ,SOD和GSH Px活性降低 ,脑MAO活性增高 ,NO含量和NOS活性无明显改变。结论　D 半乳糖可以使催老大鼠氧化水平增加 ,抗氧化能力下降 ;且两者变化无性别差异。     

Increased iron and oxidative stress are separately related to cognitive decline in elderly

Aim: The aim of this study is to examine the relation between body iron, oxidative stress and cognitive function in elderly. Methods: Eighty‐seven elderly residents from nursing homes were the subjects of our study. Cognitive status was screened by the Mini‐Mental State Examination (MMSE). Of the 87 eligible subjects, 46 patients who obtained 24 or fewer points on the MMSE scale were considered as subjects with cognitive dysfunction. The control group consisted of 41 subjects who obtained more than 24 points on the MMSE. Routine biochemical analyses, parameters of iron metabolism, malondialdehyde (MDA) and glutathione peroxidase (GSH‐Px) were determined in all subjects. Results: There were statistically significant increases in serum iron, transferrin saturation, ferritin and MDA levels; whereas there was a statistically significant decrease in serum GSH‐Px enzyme activity and serum sodium levels in subjects with cognitive dysfunction. A significant negative correlation was found between serum iron, transferrin saturation, ferritin and MMSE score. There was a negative correlation between MMSE score and serum MDA; however, a positive significant correlation was found between MMSE score and both GSH‐Px enzyme activity and serum sodium. Conclusion: Our study provides evidence of increased markers of iron deposition and oxidative stress in patients with cognitive dysfunction. It seems likely that these markers negatively affect the MMSE score. Interestingly, we did not find any correlation between the markers of iron deposition and oxidative stress. Future studies will be required to demonstrate whether diminishing iron and oxidative stress will enhance MMSE score and thereby ameliorate cognitive impairment. Geriatr Gerontol Int 2011; 11: 504–509.     

大黄泻下与劳倦过度单、复因素造模脾虚小鼠过氧化与抗氧化改变的比较研究

目的：探讨单因素与复合因素造模法对脾虚动物模型生物膜结构和功能改变的规律及其机制。方法：70只小白鼠随机分为4组,即大黄泻下造模（A）组20只,劳倦过度造模（C）组20只,大黄泻上加劳倦过度造模（AC）组20只,空白对照（O）组10只。观察各组小白鼠丙二醛（MDA）、共轭双烯（CD）、过氧化氢酶（Cat)、超氧化物歧化酶（SOD）及过氧化物酶（GSH－Px）的变化,结果：3种方法塑造的脾虚小鼠模型均出现了体重下降,MDA、CD升高,Cat,SO,GSH-Px下降,且脂质过氧化损伤程度和抗氧化氧化酶活性下降程度以AC组为最重。结论：脾虚造模方法以大黄泻下加劳倦过度法为优,复因素脾虚造模法要优于单因素脾虚造模法。     

Malondialdehyde: a possible marker of ageing

BACKGROUND: Numerous recent studies have suggested that oxidative damage may be important in the ageing process, and lipid peroxidation is an important biological consequence of oxidative cellular damage. OBJECTIVE: The aim of this work was to analyze the activities of the two protective enzymes, superoxide dismutase (SOD) and catalase (CAT), and the levels of malondialdehyde (MDA) to examine the relationship between the ageing process and defence antioxidant and lipid peroxidation. METHOD: SOD activity was measured in red blood cells using the Minami and Yoshikawa method; CAT activity was measured in hemolysates by the Aebi method, and MDA levels were measured in erythrocytes by high-performance liquid chromatography. RESULTS: SOD activity shows statistically significant differences between newborns and the rest of the sample (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.001). CAT activity did not show significant differences between the age groups. We observed statistically significant differences in MDA levels between the different groups (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.05). In the regression analysis and rectilinear/curvilinear adjustment compared to age, SOD and CAT showed coefficients close to zero (SOD linear = 0.16; SOD exponential = 0.15; CAT linear = 0.056; CAT exponential = 0.068), indicating in that way their independence from age. Only MDA obtained a regression coefficient superior to 0.75 (p < 0.05). The best adjustment was reached through an exponential expression, giving the following parametric relation: MDA = 103.117e(0.0021.AGE). No statistically significant variation in SOD and CAT activity and MDA levels, related to sex could be demonstrated. CONCLUSIONS: Our data show that old age is associated with an increase in systemic oxidative stress.     

Melatonin stimulates the activity of protective antioxidative enzymes in myocardial cells of rats in the course of doxorubicin intoxication

The study aimed at determining the effect of melatonin on the activity of protective antioxidative enzymes in the heart and of lipid peroxidation products in the course of intoxication with doxorubicin (DOX). The rats were categorized into four groups, receiving: 0.9% NaCl i.p. (NaCl control); melatonin [20 mg/kg body weight (b.w.)] s.c. (control Mel); DOX (2.5 mg/kg b.w.) i.p.; melatonin plus DOX in doses as above. All the substances were administered once in a week for four consecutive weeks. Homogenates of heart tissue were examined for activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), levels of reduced glutathione (GSH) and of lipid peroxidation indices (MDA + 4-HDA). Administration of melatonin alone did not induce alterations in levels of MDA + 4-HDA, GSH, or in activity of GPx, SOD or CAT, as compared to the group receiving 0.9% NaCl. GSH levels decreased following DOX but remained at normal levels following DOX and melatonin. The level of MDA + 4-HDA increased following DOX, as compared with the control, a change prevented by the combination of DOX + melatonin. Activities of GPx, SOD and CAT were higher in groups receiving DOX and/or DOX plus melatonin than in control groups. Activity of CAT and the level of GSH in the group receiving DOX plus melatonin were significantly higher than in the group intoxicated with DOX alone. The obtained results demonstrate that, when given in parallel with DOX, melatonin protects cardiomyocytes from damaging effects of the cytostatic drug (reflected by the levels of MDA + 4-HDA). The protective effect resulted, in part from the augmented levels of GSH and from stimulation of CAT activity by melatonin in cardiomyocytes subjected to the action of DOX.     

硒和维生素E对大鼠心肌腺苷酸及氧化损伤的影响

目的：研究低硒（Ｓｅ）低维生素Ｅ（ＶＥ）饮食大鼠心肌腺苷酸含量和氧化损伤的关系。方法：使用高效液相测定心肌腺苷酸含量和使用生化方法测定心肌ＭＤＡ含量和ＳＯＤ、ＧＳＨ－Ｐｘ活性。结果：与补充Ｓｅ和／或ＶＥ饮食大鼠相比，低Ｓｅ低ＶＥ饮食大鼠心肌ＡＭＰ，ＡＤＰ含量无明显差异，而ＡＴＰ含量明显降低，ＭＤＡ含量增加，ＳＯＤ，ＧＳＨ－Ｐｘ活性降低，表明Ｓｅ和ＶＥ影响ＡＴＰ含量与其抗氧化作用有关，而其中以联合补充Ｓｅ和ＶＥ效果最佳     

家兔肝缺血再灌注损伤中脂质过氧化反应及人参多糖的干预

目的 观察肝缺血再灌注损伤时脂质过氧化的变化以及人参多糖的干预作用,并探究其机制.方法 30只家兔随机均分为对照组、缺血再灌注组和人参多糖组.观察血浆及肝组织中丙二醛含量及超氧化物歧化酶、谷胱甘肽过氧化物酶、黄嘌呤氧化酶和丙氨酸氨基转移酶活力变化,光镜下观察肝组织结构变化,并观察人参多糖对上述指标的影响.结果 缺血再灌注组血浆超氧化物歧化酶和谷胱甘肽过氧化物酶活力在肝脏缺血45 min以及再灌注45 min逐步降低,丙氨酸氨基转移酶、黄嘌呤氧化酶活力和丙二醛含量明显升高.人参多糖组血浆超氧化物歧化酶和谷胱甘肽过氧化物酶活力与缺血前比无明显下降,丙氨酸氨基转移酶、黄嘌呤氧化酶活力和丙二醛含量无明显升高,尤其再灌注45 min血浆超氧化物歧化酶活力显著高于缺血再灌注组同期(P<0.01),丙氨酸氨基转移酶、黄嘌呤氧化酶活力和丙二醛含量显著低于缺血再灌注组同期水平(P<0.01).肝组织超氧化物歧化酶、谷胱甘肽过氧化物酶活力缺血再灌注组明显低于对照组,人参多糖组则明显高于缺血再灌注组(P<0.01);黄嘌呤氧化酶活力和丙二醛含量缺血再灌注组明显高于对照组(P<0.05或P<0.01),而人参多糖组则明显低于缺血再灌注组(P<0.05或P<0.01).光镜下发现缺血再灌注组肝组织细胞形态学结构明显异常,人参多糖组肝组织损伤明显减轻.结论 人参多糖能降低黄嘌呤氧化酶活性,减少氧自由基的生成,并且能增强超氧化物歧化酶、谷胱甘肽过氧化物酶等抗氧化酶的活性,清除氧自由基,抑制脂质过氧化反应,从而有效减轻肝缺血再灌注损伤.     

α-硫辛酸降低糖尿病大鼠肾脏氧化应激水平

将大鼠分成正常对照（NC）组、糖尿病（DM）组及糖尿病加α-硫辛酸（DM＋ALA）组进行实验。4周后DM组24小时尿白蛋白（UAlb/24h）、肾重/体重（KW/BW）和丙二醛（MDA）含量均较NC组增加，总超氧化物歧化酶（TSOD）活性降低，谷胱甘肽过氧化物酶（GSH-Px）活性升高，过氧化氢酶（CAT）活性无变化；DM＋ALA组较DM组UAlb/24h、KW/BW和MDA水平降低，TSOD、GSH-Px和CAT活性无改变。结果说明，ALA能减低DM大鼠肾皮质氧化应激水平，延缓糖尿病肾病进展。