甲磺酸加替沙星对小鼠特异性免疫的调节作用研究

目的考察甲磺酸加替沙星(GFLX)对小鼠特异性免疫防御的调节作用。方法用溶血分光光度法测定GFLX对小鼠抗绵羊红细胞(SRBC)抗体产生的影响；用MTT法测定GFLX对小鼠脾淋巴细胞转化的影响；用结晶紫染色法测定GFLX对小鼠腹腔巨噬细胞产生TNF的影响；用足肿胀法测定GFLX对小鼠迟发型变态反应(DTH)的影响。结果GFLX能增加小鼠特异性抗SRBC抗体(溶血素)的产生；在0．05～40μg／ml范围内能协同刁豆蛋白(conA)或脂多糖(LPS)促进小鼠脾淋巴细胞转化；在0．01～10vg／ml范围内可抑制小鼠腹腔巨噬细胞产生TNF—α；促进小鼠DTH。结论GFLX有调节小鼠特异性免疫防御的作用。     

金花葵粗黄酮提取物的免疫调节作用研究

目的:研究金花葵粗黄酮提取物对小鼠的免疫调节作用.方法:通过碳廓清实验、NK细胞实验、血清半数溶血值实验、抗体生成细胞实验、小鼠淋巴细胞转化实验、迟发性变态反应实验、免疫器官/脏体比实验考察金花葵粗黄酮提取物的免疫调节作用.结果:金花葵粗黄酮提取物可以增强小鼠巨噬细胞的吞噬百分率、单核吞噬细胞的吞噬指数、NK细胞活性和脾淋巴细胞增殖能力,增加脾抗体形成细胞和血清半数溶血值,但对小鼠足跖厚度及免疫器官脏体比值没有影响.结论:金花葵粗黄酮提取物具有一定的调节免疫的作用.     

银杏叶提取物对小鼠免疫功能的影响

目的:了解银杏叶提取物对小鼠免疫功能的影响。方法:采用体外抗体形成细胞检测法检测银杏叶提取物对小鼠脾细胞产生抗体的影响;采用间接免疫荧光法测定银杏叶提取物对小鼠CD4+/CD8+T淋巴细胞亚群的影响;采用3 H-TdR掺入法检测银杏叶提取物对小鼠淋巴细胞转化活性的影响。结果:银杏叶提取物有促进小鼠体液免疫功能的作用(P<0.01);能通过调整CD4+/CD8+T细胞亚群的比值来增强小鼠免疫功能(P<0.05);能增加小鼠淋巴细胞转化活性(P<0.001)。结论:银杏叶提取物能增强小鼠免疫功能。     

芍芪多苷对小鼠体液免疫的调节作用

目的:探讨在不同免疫状态下,芍芪多苷(SQDG)对小鼠体液免疫的调节作用.方法:采用绵羊红细胞(SRBC)诱导小鼠产生SRBC抗体模型及环磷酰胺(Cy)诱导小鼠免疫增强和抑制模型.小鼠连续灌胃SQDG 7d.采用MTT法检测脂多糖(LPS)诱导的小鼠胸腺B淋巴细胞增殖,分光光度法测小鼠血清半数溶血值(HC50)和小鼠脾细胞IgM抗体生成水平.结果:SQDG(120 mg·kg-)灌胃给药7d能提高正常小鼠的胸腺指数、脾脏指数、血清HC50、脾脏B细胞增殖和脾细胞IgM水平,SQDG(60,120 mg·kg-1)对Cy(150mg·kg-1)诱导的免疫低下小鼠的胸腺指数、脾脏指数、血清Hc50、脾脏B细胞增殖、脾细胞IgM水平具有明显的恢复作用,SQDG(60,120 mg·kg-1)对Cy(250 mg·kg-1)诱导的免疫亢进小鼠的胸腺指数、脾脏指数、血清HC50、脾脏B细胞增殖、脾细胞IgM水平均有下调作用.结论:SQDG对小鼠体液免疫反应具有双向调节作用.     

小鼠树突状细胞过继转移免疫作用的研究

目的 探讨树突状细胞（DCs)在过继免疫中的作用。以及递呈不同性质抗原的特点,为应用DCs诱导免疫耐受或治疗肿瘤奠定基础。方法 分别用绵羊红细胞（SRBC）和人白蛋白单次免疫和再次免疫小鼠,分离脾DCs和腹腔巨噬细胞（Mφ）,并分别转移至各组小鼠体内,检测抗SRBC和抗人白蛋白抗体效价。结果 实验组中不论是DCs组,还是Mφ组产生的抗体效价均较高于对照组。过继转移白蛋白时,DCs组产生抗体水平较Mφ组低;过继转移SRBC时,DCs组产生的抗体水平较Mφ组稍高,但无统计学意义,而血清中的抗体水平与抗体形成细胞（AFC）水平发生分离。结论 DCs既能处理递呈可溶性抗原,又具有较强的处理,递呈颗粒性抗原通过过继转移给受体,使受体T、B细胞活化。     

裂裥多糖处理的巨噬细胞及其对同系与异系肿瘤细胞的抗瘤活性Ⅰ.SPG处理的巨噬细胞的特性

裂裥多糖(Schizophyllan,SPG)是从裂裥菌(Schizophylum commune Fries)培养液中提取的葡聚糖。它可兴奋巨噬细胞,但对小鼠脾细胞及胸腺细胞在5种浓度下均无诱导有丝分裂的作用,这与短棒菌苗、卡介苗、壁酰二肽(MDP)有明显差别。用抗Thy1,2的单克隆抗体加豚鼠补体处理SPG 兴奋过的腹腔渗出     

903中药复方煎剂对小鼠体液免疫的增强作用

903中药复方煎剂对小鼠脾细胞的PFC数、血清溶菌酶活性和抗SRBC的溶血性抗体水平均有明显增强,尤其是PEC数比正常对照组的增强更为明显(P<0.01)。此外,本煎剂能对抗环磷酰胺(CY)对小鼠免疫功能的抑制作用。提示903中药复方煎剂对小鼠的体液免疫有增强作用。     

苦杏仁甙对小鼠免疫功能的影响

以不同剂量的苦杏仁甙（１．５、３、５ｍｇ／只）经肌肉注射给予小鼠后，取其脾脏制成脾细胞悬液，进行Ｔ淋巴细胞转化实验，观察苦杏仁甙对小鼠脾细胞增殖反应的影响。结果表明，苦杏仁甙能明显促进有丝分裂原对小鼠脾脏Ｔ淋巴细胞的增殖。     

新合成的免疫增强剂:NPT15392

NPT15392是肌苷类的含氮杂环化合物,经实验证明能调节淋巴细胞和巨噬细胞的功能。它在体外能增加人外周血淋巴细胞中活性花结比例,但不影响总花结。它还能促进致有丝分裂原和抗原诱发的淋巴细胞增生作用,增强Con A对抑制性T细胞的诱导,但对巨噬细胞的细胞毒作用无影响。在实验动物中,它能增强小鼠脾细胞对PHA和LPS的反应。它还具有胸腺素样的作用,与nu/nu小鼠脾细胞的尼龙不粘附部分一起培养后,能象胸腺素一样显著增加Thy1阳性细胞数。它对小鼠巨噬细胞的细胞毒作用也无影响。动物体     

八宝颗粒剂对小鼠免疫功能的影响

目的研究康姜牌八宝颗粒剂对正常小鼠免疫功能的影响。方法将小鼠随机分为两组比较疗效。治疗组口服给予不同剂量的八宝颗粒剂（分别为2000mg／（kg·bw）、4000mg／（kg·bw）和12000mg／（kg·bw）），对照组采用生理盐水灌服。观察八宝颗粒剂对小鼠刀豆球蛋白（ConA）诱导的脾淋巴细胞转化、抗体生成细胞（溶血空斑数）、NK细胞的作用。结果康美牌八宝颗粒剂可促进ConA诱导小鼠脾淋巴细胞的增殖．促进小鼠抗体生成细胞的形成，提高NK细胞的活性。结论康美牌八宝颗粒剂可明显增强小鼠免疫能力。     

The effect of salazosulfapyridine on the in vitro antibody production in murine spleen cells

The effect of salazosulfapyridine (SASP) on the antibody response of murine spleen cells in vitro was studied. SASP inhibited the response to sheep red blood cells (SRBC), a T-cell-dependent (TD) antigen, dose-dependently and was most effective at a dose of 2 x 10(-4) M without cell toxicity. No remarkable inhibition was seen with the main metabolites of SASP, 5-aminosalicylic acid (5-ASA) and sulfapyridine (SP). SASP failed to inhibit antibody production to T-cell-independent antigens such as dinitrophenyl-Ficoll or trinitrophenyl (TNP)-lipopolysaccharides, although the response to TNP-keyhole limpet hemocyanin, another TD antigen like SRBC, was inhibited. Further, this drug did not show any depression of the anti-SRBC plaque-forming cell (PFC) response in spleen cells treated with anti-Thy1.2 antibody plus complement. The inhibition of anti-SRBC PFC response by SASP was accompanied by a reduction of interleukin 2 (IL-2) secretion. Our results suggest that SASP may act on T cell populations and may inhibit the T-cell-dependent antibody response partly through a depression of IL-2 production. The active compound appears to be SASP itself, rather than its metabolites.     

南蛇藤乙醇提取物的免疫调节作用研究

目的:研究南蛇藤乙醇提取物的免疫调节作用。方法:采用小鼠单核巨噬细胞实验,以廓清指数为指标评价南蛇藤乙醇提取物对非特异性免疫功能的作用;采用小鼠溶血素实验,以小鼠溶血素为指标评价南蛇藤乙醇提取物对特异性免疫功能的作用。结果:南蛇藤乙醇提取物(60g·kg-1)可显著降低小鼠碳廓清指数(P<0.05)。南蛇藤乙醇提取物(60、20g·kg-1)对绵羊红细胞免疫小鼠溶血素抗体的生成有减少的趋势,但无显著性意义。结论:南蛇藤乙醇提取物具有降低非特异性免疫功能的药理作用,对特异性免疫功能没有显著影响。     

Streptovirudins, new antibiotics with antibacterial and antiviral activity. I. Culture taxonomy, fermentation and production of streptovirudin complex.

A new antibiotic complex has been isolated from cultures of Streptomyces strain No. JA 10124. On the basis of taxonomic studies, the producing microorganism is described as Streptomyces griseoflavus (Krainsky, 1914) Waksman et Henrici, 1948, subsp. thuringiensis subsp. nov., type strain JA 10124. The antibiotic complex, designated as streptovirudin, was isolated from extracts of both mycelium and culture filtrate. It is a white amorphous material which consists of ten closely related components including streptovirudins A, B, C, D and E. The streptovirudin complex exhibits antibiotic activity against Gram-positive bacteria, mycobacteria, and various DNA- and RNA-viruses.     

Direct suppression of cultured spleen cell responses by chlordane and the basis for differential effects on in vivo and in vitro immunocompetence

Immunosuppression by gamma-chlordane was examined by the direct addition of chlordane to cultured spleen cells from untreated B6C3F1 mice. Both cell-mediated and humoral immune responses were markedly suppressed upon in vitro exposure. The mixed lymphocyte response and the proliferative response to both B- and T-cell mitogens were significantly suppressed at micromolar concentrations of chlordane. The antibody response to sheep erythrocytes (SRBC) was suppressed 90% at 10 microM chlordane. The kinetics of the SRBC response were not altered by chlordane. Addition of chlordane to the antibody cultures on various days indicated an effect at the early stages of the response. Previous studies with chlordane failed to demonstrate immunosuppression following in vivo exposure. The possibility that chlordane was metabolized in vivo to a less immunosuppressive form was studied by examining the effect of the major metabolite, oxychlordane, on the in vitro antibody response and by incubating splenocytes with chlordane and a liver S9 preparation prior to culture with SRBC. Oxychlordane was immunosuppressive by itself, and the activity of chlordane was unaltered in the co-culture experiments. The association of chlordane with serum components was evaluated in vitro in cultures of mouse bone-marrow cells (BMC). The chlordane-induced suppression of [3H]thymidine incorporation by BMC was reversed by the addition of mouse or human serum. In summary, chlordane produces marked suppression of in vitro immune responses via an apparent antiproliferative action. The failure of chlordane to produce in vivo immunosuppression may be related to extensive association of chlordane with serum components.     

白细胞介素7基因转染口腔癌细胞株的免疫调节效应的研究

目的：探讨IL-7基因转染人舌鳞癌Tca8113细胞株的免疫调节效应。方法：将已构建的原核／真核表达载体pBK—CMV／IL-7转染到癌细胞株中，采用MTT法检测转染前后肿瘤细胞培养上清对鼠脾细胞增殖的影响；ELISA法检测细胞培养上清转移生长因子β1（TGF-β1）、血管内皮生长因子（VEGF）和IL-10的浓度。将pPK—CMV／IL-7癌细胞株移植于小鼠腹腔，观察肿瘤细胞的成瘤性；并采用MTT法检测其NK杀伤活性。结果：IL-7基因转染癌细胞株的培养上清对鼠脾细胞的增殖抑制作用与对照组比较显著下降（P〈0．05），并且IL-7基因转染的肿瘤细胞产生TGF-β1、VEGF和IL-10三种免疫抑制因子明显降低（P〈0．05）；IL-7基因转染组鼠腹腔瘤结节明显小于对照组（P〈0．05）；NK的杀伤活性与对照组比较，具统计学差异（P〈0．05）。结论：IL-7基因转染人舌鳞癌细胞株具有抑瘤作用，其机理可能是通过降低肿瘤细胞产生免疫抑制因子，进而促进淋巴细胞增殖，提高NK的杀伤活性．     

葛根素对小鼠皮肤来源朗格汉斯细胞成熟分化及炎症因子分泌的影响

目的：观察葛根素（puerarin）对小鼠皮肤来源朗格汉斯细胞数量、成熟及炎症因子分泌的影响,以期探讨葛根素在炎症相关性疾病治疗中的潜在机制。方法：葛根素作用于体外培养的C57BL/6小鼠皮肤朗格汉斯细胞后,使用流式细胞术检测葛根素对朗格汉斯细胞数量、抗原捕获、MHC-Ⅱ表达等成熟能力的影响;同时利用小鼠细胞因子抗体芯片技术检测培养上清中细胞因子的变化。结果：葛根素作用后,小鼠朗格汉斯细胞数量无明显变化,但抗原捕获能力显著增加;MHC-Ⅱ^high亚群细胞比例降低,MHC-Ⅱ^low亚群细胞比例则增多;MHC-Ⅱ⁺CD80⁺和MHC-Ⅱ⁺CD86⁺细胞比例显著降低;培养上清中IL-1β、MCP-1的分泌也受到明显抑制。结论：葛根素抑制朗格汉斯细胞成熟能力和炎症因子分泌水平,有助于阐明朗格汉斯细胞在炎症相关性疾病中的作用机制具有重要临床意义。     

Ripromycin and other polycyclic macrolactams from Streptomyces sp. Tü 6239: taxonomy,fermentation, isolation and biological properties

Strain Tü 6239 was isolated from a soil sample collected in Brazil and determined as a new species of the genus Streptomyces. In the course of our HPLC-diode array screening program three metabolites were detected in the culture filtrate and mycelium extracts of strain Tü 6239. They were characterised as members of the macrolactam group, the new compound ripromycin (1), the previously described ikarugamycin (2) and a new derivative of it, ikarugamycin epoxide (3). They show antibiotic activities against gram-positive bacteria and cytostatic effects to various human tumor cell lines.     

Metabolic products of microorganisms. 244. Colabomycins, new antibiotics of the manumycin group from Streptomyces griseoflavus. I. Isolation, characterization and biological properties

The yellow colabomycins A to C, three new antibiotics of the manumycin group produced by Streptomyces griseoflavus (strain Tü 2880), were detected by chemical screening. They were isolated from mycelium extracts by column chromatography on various adsorbents, followed by preparative reversed phase HPLC. The main compound, colabomycin A (1), was characterized and shown to be chiefly biologically active against Gram-positive bacteria and stem cells of murine L1210 leukemia.     

Effects of an extract of feverfew on endothelial cell integrity and on cAMP in rabbit perfused aorta

Extracts of feverfew inhibit platelet aggregation and secretion of granular contents from platelets and other cells. They also modify the interaction of platelets with collagen substrates: feverfew extracts inhibit both platelet spreading and formation of thrombus-like platelet aggregates on the collagen surface. We have now investigated the effect of an extract of feverfew on the vessel wall using rabbit aortas that were perfused with a physiological salt solution in-situ. Addition of feverfew extract to the perfusion medium protected the endothelial cell monolayer from perfusion-induced injury and led to a reversible increase in the cAMP content of aorta segments. The results indicate that feverfew may have a vasoprotective effect in addition to its effects on platelets.