miR-342-3p对三阴性乳腺癌化疗敏感性的影响

目的:研究miR-342-3p对三阴性乳腺癌化疗敏感性的影响.方法:收集江苏省肿瘤医院乳腺外科2011年1月至2013年8月行术前化疗的三阴性乳腺癌病人32例,其中完全缓解(CR)5例,部分缓解(PR)27例,检测肿瘤组织中miR-342-3p的表达情况;应用脂质体转染方法,三阴性乳腺癌细胞株MDA-MB-231转染has-miR-342-3p模拟物(mimic)和miR-342-3p抑制物(inhibitor),设立阴性对照(mim-NC)组和(inhi-NC)组.mimic组,mim-NC组,inhibitor组和inhi-NC组细胞株,分别加入浓度为2μmol/L的紫杉醇,顺铂及4μmol/L的阿霉素进行培养;应用CCK8法和流式细胞仪检测48 h后肿瘤细胞增殖率和凋亡的变化.结果:miRNA-342-3p在术前化疗后达到CR的三阴性乳腺癌组织中的表达明显高于PR的三阴性乳腺癌组织(P＜0.05).Mimic组细胞株与紫杉醇,顺铂作用48 h后肿瘤细胞增殖率低于mim-NC组,凋亡率高于mim-NC组,差异有统计学意义(w0.05).Inhibitor组细胞株与紫杉醇,顺铂作用48 h后,肿瘤细胞增殖率高于inhiNC组,凋亡率低于inhi-NC组,差异有统计学意义(P＜0.05).但与阿霉素作用后细胞增殖率和凋亡率在mimic组与mimNC组,inhibitor组与inhi-NC组,差异无统计学意义(P＞0.05).结论:miR-342-3p高表达的三阴性乳腺癌对化疗药物反应更敏感,miR-342-3p能调控乳腺癌细胞株MDA-MB-231对紫杉醇和顺铂的化疗敏感性,但miR-342-3p不影响MDAMB-231细胞株对阿霉素的化疗敏感性.     

miR-18b增强乳腺癌MDA-MB-231细胞对顺铂化疗敏感性的作用及其机制

目的:探讨miR-18b在三阴性乳腺癌MDA-MB-231细胞对顺铂(DDP)化疗敏感性中的作用,阐明其可能的机制。方法:乳腺癌MDA-MB-231细胞根据转染条件分为MDA-MB-231组、MDA-MB-231+DDP组、MDA-MB-231-NC组、MDA-MB-231-NC+DDP组、MDA-MB-231 mimic组和MDA-MB-231 mimic+DDP组。采用生物信息学方法预测的miR-18b的靶基因;荧光素酶报告基因分析验证miR-18b与靶基因3'UTR的结合;应用qRT-PCR法检测各组MDA-MB-231细胞中miR-18b的表达水平;Western blotting法检测MDA-MB-231细胞中ATM蛋白表达水平;CCK-8法检测各组细胞的生长抑制率。结果:荧光素酶实验报告验证ATM为miR-18b的靶基因,并与ATM 3'UTR序列部分结合;与MDA-MB-231-NC组比较,MDA-MB-231 mimic组和MDA-MB-231 mimic+DDP组细胞中miR-18b的表达水平升高(P<0.05);Western blotting法检测,与MDA-MB-231组比较,MDA-MB-231+DDP组细胞中ATM蛋白表达水平升高(P<0.05);与MDA-MB-231-NC组比较,MDA-MB-231 mimic组细胞中ATM蛋白表达水平降低(P<0.05);与MDA-MB-231-NC+DDP组比较,MDA-MB-231 mimic+DDP组细胞中ATM蛋白表达水平降低(P<0.05);CCK-8法检测,与MDA-MB-231 NC组比较,经不同浓度DDP作用后,MDA-MB-231 mimic组细胞生长抑制率均升高。结论:miR-18b通过靶向调控ATM蛋白增强乳腺癌MDA-MB-231细胞对DDP的化疗敏感性。     

微小核糖核酸101异常表达与乳腺癌MDA-MB-231细胞增殖的关系

目的探讨微小核糖核酸101（miRNA-101）在人乳腺癌MDA-MB-231细胞中的表达及其对MDAMB-231 细胞增殖的影响。方法实时荧光定量聚合酶链反应（qRT-PCR）检测乳腺癌细胞MDA-MB-231和正常乳腺上皮细胞MCF-10A 中miRNA-101 的表达。采用Lipofectamine TM 2000 将miRNA-101-mimic/inhibitor/NC 分别转染至MDA-MB-231 细胞中,通过qRT-PCR检测miRNA-101 的转染效率,CCK-8 实验检测MDA-MB-231 细胞的增殖。结果miRNA-101 在MDA-MB-231 细胞中的表达水平低于正常乳腺细胞MCF-10a（ p<0.01）。转染miRNA-101 mimic 后MDA-MB-231 细胞的增殖能力减弱（p <0.05）,而转染miRNA-101 inhibitor后细胞的增殖能力增强（p <0.05）。结论miRNA-101 在乳腺癌细胞MDA-MB-231中低表达,转染miRNA-101 mimic后乳腺癌细胞MDA-MB-231 的增殖减弱。     

miR-376c-3p通过靶向谷氨酸受体相互作用蛋白1影响乳腺癌细胞的增殖和迁移

目的:探讨miR-376c-3p在乳腺癌中的表达水平、生物学功能及分子调控机制.方法:通过实时荧光定量PCR检测乳腺上皮MCF-10A细胞及乳腺癌细胞中miR-376c-3p表达水平;然后,用miR-376c-3p mimic转染乳腺癌MCF-7与MDA-MB-231细胞,用平板克隆实验、划痕实验以及Transwell...     

MiR-152-3p靶向PIK3CA基因对乳腺癌细胞生物学特性的调控及其机制

目的探讨miR-152-3p靶向PIK3CA基因对乳腺癌细胞生物学特性的调控及机制。方法选取2016年1月至2017年7月宣城市中心医院保存的乳腺癌组织和相应的正常乳腺组织(距肿瘤边缘> 5 cm)标本各50例,培养乳腺癌MCF-7细胞和正常乳腺上皮细胞MCF-10A,采用反转录定量聚合酶链反应(qRT-PCR)检测乳腺癌组织、正常乳腺组织、MCF-7细胞及MCF-10A细胞中miR-152-3p及PIK3CA mRNA表达。培养MCF-7细胞,待细胞融合度达30%～50%时将细胞分为空白组、阴性对照组、miR-152-3p mimic组、miR-152-3p inhibitor组、si-PIK3CA组和miR-152-3p inhibitor+si-PIK3CA组,空白组细胞不转染任何序列,阴性对照组细胞转染miR-152-3p阴性对照质粒,miR-152-3p mimic组细胞转染miR-152-3p mimic质粒,miR-152-3p inhibitor组细胞转染miR-152-3p inhibitor质粒,si-PIK3CA组细胞转染si-PIK3CA质粒,miR-152-3p inhibitor+si-PIK3CA组细胞共转染miR-152-3p inhibitor质粒和si-PIK3CA,转然后继续培养24～48 h;采用Western blot法检测各组MCF-7细胞中PIK3CA、细胞外信号调节激酶(ERK)、c-Jun氨基末端激酶(JNK)、p38和E-cadherin蛋白表达,四甲基偶氮唑盐法检测各组MCF-7细胞增殖活性,划痕实验检测各组MCF-7细胞迁移能力,Transwell侵袭实验检测各组MCF-7细胞侵袭能力。结果乳腺癌组织中miR-152-3p相对表达量显著低于正常乳腺组织,PIK3CA mRNA相对表达量显著高于正常乳腺组织(P <0. 05)。MCF-7细胞中miR-152-3p相对表达量显著低于MCF-10A细胞,PIK3CA mRNA相对表达量显著高于MCF-10A细胞(P <0. 05)。与空白组和阴性对照组比较,miR-152-3p mimic组和si-PIK3CA组MCF-7细胞中PIK3CA、p38、ERK、JNK和E-cadherin蛋白相对表达量显著降低,miR-152-3p inhibitor组MCF-7细胞中PIK3CA、p38、ERK、JNK和E-cadherin蛋白相对表达量显著升高(P <0. 05)。miR-152-3p inhibitor+si-PIK3CA组与空白组和阴性对照组MCF-7细胞中PIK3CA、p38、ERK、JNK和E-cadherin蛋白相对表达量比较差异无统计学意义(P>0. 05)。细胞增殖能力实验结果显示,48、72、96 h时,miR-152-3p mimic组和si-PIK3CA组MCF-7细胞增殖能力显著低于空白组和阴性对照组,miR-152-3p inhibitor组MCF-7细胞增殖能力显著高于空白组和阴性对照组(P <0. 05); 48、72、96 h时,miR-152-3p inhibitor+si-PIK3CA组与空白组和阴性对照组MCF-7细胞增殖能力比较差异均无统计学意义(P> 0. 05)。划痕实验和Transwell侵袭实验结果显示,空白组与阴性对照组MCF-7细胞迁移和侵袭能力比较差异无统计学意义(P> 0. 05);与空白组和阴性对照组比较,miR-152-3p mimic组和si-PIK3CA组MCF-7细胞迁移和侵袭能力显著降低,miR-152-3p inhibitor组MCF-7细胞迁移和侵袭能力显著增强(P <0. 05); miR-152-3p inhibitor+si-PIK3CA组与空白组和阴性对照组MCF-7细胞迁移和侵袭能力比较差异无统计学意义(P> 0. 05)。结论 miR-152-3p在乳腺癌组织中低表达,miR-152-3p可能参与了乳腺癌的发生与发展,miR-152-3p可能通过抑制PIK3CA表达而抑制MAPK信号通路的激活,进而抑制乳腺癌细胞的生长、增殖及侵袭转移。     

miR-145对人乳腺癌MCF-7/ADR细胞耐药逆转的作用机制研究

目的:探讨miR-145对人乳腺癌阿霉素耐药细胞株MCF-7/ADR阿霉素耐药的影响及可能的作用机制。方法:采用实时定量PCR方法检测miR-145在乳腺癌细胞株MCF-7及MCF-7/ADR中的表达差异;脂质体转染法将构建好的miR-145 mimics,miR-145inhibitor成功转染进MCF-7/ADR细胞中,MTT法检测转染后MCF-7/ADR细胞对阿霉素的敏感性,流式细胞仪检测耐药细胞对阿霉素诱导凋亡的影响,Western blot检测转染前后抗凋亡蛋白Bcl-2、多药耐药基因MDR1表达蛋白P-gp的表达差异。结果:miR-145在人乳腺癌细胞株MCF-7/ADR中表达下降;上调miR-145可以增强MCF-7/ADR细胞对阿霉素的敏感性,显著抑制MCF-7/ADR细胞增殖,并促进阿霉素诱导的细胞凋亡,同时显著抑制了耐药细胞中Bcl-2和P-gp的表达。结论:miR-145通过抑制Bcl-2和P-gp蛋白的表达来增加MCF-7/ADR细胞对阿霉素的敏感性和凋亡。     

MiR-671-3p靶向DEPTOR而抑制乳腺癌细胞的增殖与侵袭

目的 研究miR-671-3p对乳腺癌细胞增殖、侵袭生物学功能的影响及其可能的作用机制。方法 以实时荧光定量PCR方法检测正常乳腺上皮细胞（MCF-10A）与乳腺癌细胞（MCF-7、MDA-MB-231、SK-BR3）中miR-671-3p表达情况;检测miR-671-3p 在MCF-7细胞中的转染效率;通过CCK-8法检测miR-671-3p对MCF-7细胞增殖的影响;通过Transwell实验、划痕实验检测miR-671-3p对MCF-7细胞侵袭迁移能力的影响;Targetscan预测miR-671-3p的靶基因,并通过双荧光素酶法和Western blot法对靶基因DEPTOR进行验证,并检测DEPTOR的表达。结果 miR-671-3p在乳腺癌细胞系MCF-7、MDA-MB-231、SK-BR3的表达量低于乳腺正常上皮细胞MCF-10A的表达。通过mimics、inhibitor分别促进、抑制miR-671-3p在MCF-7细胞中的表达后,结果显示miR-671-3p对乳腺癌细胞的增殖有抑制作用（P<0.05）,对肿瘤细胞的侵袭和迁移有抑制作用（P<0.05）。双荧光素酶结果 显示DEPTOR蛋白为miR-671-3p的直接作用靶点,过表达miR-671-3p可直接抑制DEPTOR蛋白的表达。结论 miR-671-3p直接靶向作用于DEPTOR蛋白从而抑制乳腺癌细胞的增殖和侵袭。     

miR-574-3p在三阴性乳腺癌细胞紫杉醇药物敏感性中的作用

目的 研究miR-574-3p在三阴性乳腺癌细胞紫杉醇（PTX）药物敏感性中的作用。方法 构建稳定转染细胞株,根据细胞转染情况分为未转染组、miR-阴性对照（miR-NC）组和miR-574-3p过表达组,细胞计数试剂盒8(CCK-8)法检测不同浓度紫杉醇作用下细胞增殖情况（未转染组设置亚组：空白组和对照组）,实时荧光定量聚合酶链式反应（PCR）法检测色素框同源物2(CBX2) mRNA的表达水平,Western blot法检测CBX2蛋白的表达水平,Transwell检测细胞侵袭迁移力,划痕实验检测细胞迁移能力。结果 CCK-8法检测结果显示,紫杉醇对各组三阴性乳腺癌MDA-MB-231细胞的生长抑制呈一定的浓度依赖性,但不同浓度紫杉醇（0.05、0.15、0.45、1.35、4.05、8.10μmol/L）作用下,miR-574-3p过表达组生长抑制作用均高于对照组与miR-NC组（P<0.05）;实时荧光定量PCR结果显示,2.10μmol/L紫杉醇作用下,miR-574-3p过表达组细胞中CBX2 mRNA表达水平低于未转染组和miR-NC组（P<0.05）;Wes...     

微RNA-342-3p/末端尿苷酰转移酶1/凝血酶敏感素-2通路在胰腺导管腺癌中的作用及机制

目的 探讨微RNA(miR)-342-3p/末端尿苷酰转移酶1(TUT1)/凝血酶敏感素-2(THBS2)通路在胰腺导管腺癌中的作用及miR-342-3p、TUT1、THBS2 mRNA表达与患者临床病理学参数的关系。方法 选择2008年5月至2021年3月在遂宁市中心医院行根治性手术切除的胰腺导管腺癌标本(n=80)和对应癌旁正常组织标本(n=20)为研究对象。采用实时荧光定量聚合酶链式反应法检测胰腺导管腺癌组织和癌旁正常组织中miR-342-3p、TUT1、THBS2 mRNA的表达。将对数生长期人胰腺导管腺癌细胞株PANC-1细胞分为对照组(转染miR-NC)、miR-342-3p过表达组(转染miR-342-3p mimic)、miR-342-3p抑制剂组(转染miR-342-3p inhibitor)、Vector组(转染pCS4-HA vectors)、TUT1组(转染pcDNA3-Flag-TUT1)、阴性对照组(转染sh-NC慢病毒载体)、TUT1沉默组(转染pGCshL-TUT1载体)、miR-342-3p过表达+TUT1组(同时转染miR-342-3p mimic和...     

MiR-4443通过抑制PEBP1的表达促进乳腺癌细胞的迁移和侵袭

目的 探讨miR-4443对乳腺癌转移的影响。方法 使用高通量测序技术测定检测3例乳腺癌患者原位癌组织及其配对的癌旁组织中miR-4443的表达。使用TCGA数据库验证miR-4443在乳腺癌组织以及正常乳腺组织中的表达水平。以MCF-7细胞（低侵袭性乳腺癌细胞）和MDA-MB-231细胞（高侵袭性乳腺癌细胞）为研究对象,采用RT-qPCR验证miR-4443在这两种细胞株中的表达水平。通过电转染法将miR-4443 mimics（miR-4443模拟物）、mimics-NC（miR-4443模拟物的对照物）或miR-4443inhibitors（miR-4443抑制物）、inhibitors-NC（miR-4443 抑制物的对照物）转染至不同的细胞株,利用RT-qPCR检测miR-4443在转染前后的表达水平,使用流式细胞分析仪分析 miR-4443 表达水平的变化对乳腺癌细胞凋亡的影响,使用划痕实验和Transwell侵袭实验检测miR-4443表达水平的变化对乳腺癌细胞迁移和侵袭能力的影响。使用生物信息学软件预测miR-4443潜在的靶基因,并进一步使用双荧光素酶报告基因系统验证。采用RT-qPCR和Western blot分别检测不同细胞株中PEBP1（重组人磷脂酰乙醇胺结合蛋白1）在mRNA和蛋白水平的表达。结果 高通量测序技术检测发现在乳腺癌组织中miR-4443的表达远高于癌旁组织（P<0.01）。TCGA数据分析显示,与正常乳腺组织想比,miR-4443在乳腺癌组织中的表达升高（P<0.01）。RT-qPCR显示,与MCF-7细胞相比,MDA-MB-231细胞中miR-4443的表达升高（P<0.01）。与转染mimics-NC相比,MCF-7细胞转染miR-4443 mimics后,miR-4443的表达上调（P<0.01）;而转染miR-4443 inhibitors的MDA-MB-231细胞,其表达下调（P<0.01）。转染miR-4443 mimics的MCF-7细胞的凋亡率与阴性对照和空白对照差异无统计学意义（P>0.05）,并且转染miR-4443 inhibitors的MDA-MB-231细胞的凋亡率与阴性对照和空白对照亦差异无统计学意义（P>0.05）。划痕实验及Transwell侵袭实验显示转染miR-4443 inhibitors的MDA-MB-231细胞的迁移及侵袭能力减弱（P<0.01）。相反的,转染miR-4443 mimics的MCF-7细胞迁移及侵袭能力加强（P<0.01）。生物信息学软件预测发现PEBP1可能是miR-4443的潜在靶基因且与转移的相关程度最高。进一步行双荧光素酶报告基因实验,验证PEBP1是miR-4443的靶基因（P<0.01）。RT-qPCR和Western blot显示,MDA-MB-231细胞中PEBP1在mRNA及蛋白中的水平均低于MCF-7细胞（P<0.01）。转染miR-4443 mimics的MCF-7细胞中,PEBP1 在mRNA及蛋白水平均低于对照（P<0.01）;转染miR-4443 inhibitors的MDA-MB-231细胞中,PEBP1 在mRNA及蛋白水平均高于对照（P<0.01）。结论 miR-4443通过抑制PEBP1的表达水平促进乳腺癌细胞的迁移和侵袭能力,而抑制乳腺癌细胞中miR-4443的表达可能是未来潜在的治疗方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.