Study on Lymphokine-Activated Killer Cells Induced from Cord Blood

ThemethodofInducingl仰phoklne—activatedkiller（LAK）cellsfromcordbloodlpophocytes,thekillingabilityof叭K比115IOin＊01皿M1血edISK562血dR叭thecomparisonoftheXillingactivitytotumorsubculturecellsamongLAK优11sfromcordbloodlymphocytes,adult’sPenPheralbloodls     

Immunomagnetic Indirect Positive Sorting of Precartilaginous Stem Cells from Neonatal Rat

The repair of bone and cartilage defects has been a tough problem in the clinical practice and it is difficult because of the lack of precursors that are able to prolif-erate in large quantity. Use of precartilaginous stem cells can solve this problem. A simple method is urgently needed to obtain high-purity precartilaginous stem cells effectively and efficiently. In this study, we used immu-nomagnetic beads to separate precartilaginous stem cells from neonatal rats with monoclone antibody of …     

Comparative Potency of Seven Compounds Isolated from TripterygiumWilfordii on Cultred Rat Leydig Cells and Sertoli Cells

The damaging effect of seven compounds isolated from Triptervgium Witfordii Hook. F, were tested on the rat Leydig cells and Sertoli cells in culture. The results showed that 10μg/ ml orthosphenic acid (TW2), 10μg/ ml 3β,22α-dihydroxy-△^12 ursen-30-oic acid(TW5), 5.0 μg/ ml 3β,22α -dihydroxy-△^12-oleanen-29-oieaeid(TW6), 5.0μg/ ml salaspermic acid(TW7), 0.5μg/ mt eelastrol(TW27) and 1.25μg/ml polpunonic acid (TW28) were all detrimental to Leydig cells and Sertoti eells. However, 30μg/ ml wilJbrlideA (TW1) did not affect the viability of Leydig cells and Sertoli cells significantly. The most toxic compounds was TW27, and the least, TW1. These results suggested that the -COOH at the C-20 site of these triterpenoids mat, be tbe funetional chemical group responsible for the damaging effect on the Leydig cells and Sertoli cells.     

Effect of Propofol on Glutamate and γ-aminobutyric Acid Release from Rat Hippocampal Synaptosomes

To investigate the effect of propofol on the release of glutamate and γ-aminobutyric acid （GABA） from rat hippocampal synatosomes, synaptosomes was made from hippocampus and incubated with artificial cerebrospinal fluid （aCSF）. With the experiment of Ca^2＋-dependent release of glutamate and GABA, dihydrokainic acid （DHK） and nipectic acid were added into aCSF. For the observation of Ca^2＋-independent release of glutamate and GABA, no DHK, nipectic acid and Ca^2＋ were added from aCSF. The release of glutamate and GABA were evoked by 20 μmol/L veratridine or 30 mmol/L KCh The concentration of glutamate and GABA in aCSF was measured by using high-performance liquid chromatography （HPLC）. 30, 100 arid 300 μmol/L propofol significantly inhibited veratridine-evoked Ca^2＋-dependent release of glutamate and GABA （P〈0. 01 or P〈0. 05）, However, propofol showed no effect on elevated KCl-evoked Ca^2＋-dependent release of glutamate and GABA （P〉0, 05）, Veratridine or elevated KCI evoked Ca^2＋-independent release of glutamate and GABA was not affected significantly by propofol （P〉0.05）. Propofol could inhibit Ca^2＋- dependent release of glutamate and GABA, However, it has no effect on the Ca^2＋-independent release of glutamate and GABA,     

The Origin of Neointimal Smooth Muscle Cells in Transplant Arteriosclerosis from Recipient Bone-marrow Cells in Rat Aortic Allograft

In order to investigate the origin of neointimal smooth muscle cells in transplant arterio- sclerosis in rat aortic allograft, sex-mismatched bone marrow transplantation was performed from male Wistar rats to female Wistar rats. Four weeks after transplantation, the aortic transplant model was established by means of micro-surgery in rats. The recipients were divided into 4 groups: female Wistar-female Wistar aortic isografts, female SD-female Wistar aortic allografts, male SD-male Wis- tar aortic allografts, female SD-chimera Wistar aortic allografts. Eight weeks after transplantation, aortic grafts were removed at autopsy and processed for histological evaluation and immunohisto- chemistry. The results indicated that excessive accumulation of α-SMA-positive smooth muscle cells resulted in significant neointima formation and vascular lumen stricture in rat aortic allografts. Neointima assay revealed that the neointimal area and NIA/MA ratio of transplanted artery were sig- nificantly increased in all of aortic allograft groups as compared with those in aortic isograft group (P<0.01). Neointimal smooth muscle cells were harvested from cryostat sections of aortic allograft by microdissection method. The Sry gene-specific PCR was performed, and the result showed that a dis- tinct DNA band of 225 bp emerged in the male-male aortic allograft group and chimera aortic al- lograft group respectively, but not in the female-female aortic allograft group. It was suggested that recipient bone-marrow cells, as the origin of neointimal smooth muscle cells, contributed to the pathological neointimal hyperplasia of aortic allograft and transplant arteriosclerosis.     

Experimental Study on Apoptosis Induced by Ursolic Acid Isolated from Asparagusin HL-60 Cells

Experimental Study on Apoptosis Induced by Ursolic Acid Isolated from Asparagusin HL-60 Cells@黄镜@孙燕@陆士新@苏涛@焦顺昌     

Effect of Amygdalin on the Proliferation of Hyperoxia-exposed Type Ⅱ Alveolar Epithelial Cells Isolated from Premature Rat

The pathogenesis of hyperoxia lung injury and the mechanism of amygdalin on type 2 alveolar epithelial cells (AEC2) isolated from premature rat lungs in vitro were investigated. AEC2 were obtained by primary culture from 20-days fetal rat lung and hyperoxia-exposed cell model was established. Cell proliferating viability was examined by MTT assay after treatment of amygdalin at various concentrations. DNA content and the proliferating cell nuclear antigen (PCNA) protein expression of AEC2 were measured by using flow cytometry and immunocytochemistry respectively after 24 h of hyperoxia exposure or amygdalin treatment. The results showed that hyperoxia inhibited the proliferation and decreased PCNA protein expression in AEC2 of premature rat in vitro. Amygdalin at the concentration range of 50--200 μmol/L stimulated the proliferation of AEC2 in a dose-dependent manner, however, 400 μmol/L amygdalin inhibited the proliferation of AEC2. Amygdalin at the concentration of 200 μmol/L played its best role in facilitating proliferation of AEC2s in vitro and could partially ameliorated the changes of proliferation in hyperoxia exposed AEC2 of premature rat. It has been suggested that hyperoxia inhibited the proliferation of AEC2s of premature rat, which may contribute to hyperoxia lung injury. Amygdalin may play partial protective role in hyperoxia-induced lung injury.     

Influence of Radix Isatidis on the Endotoxin-induced Release of TNF-α and IL-8 from HL-60 Cells

Radix Isatidisis one of the most commonlyused antipyretic and detoxicant agents in tradition-al Chinese medicine.Its original source was identi-fied to be the dried roots ofIsatidis indigoticaFort.(Cruciferae).The antipyretic and detoxicantagents in traditional Chinese medicine are a type ofsynthetic therapeutic medicine.It can comprehen-sively activate the ability of anti-infection and re-pair of the human body.There are many pharma-cological effects such as antibacteria,antivirus,antipyre…     

Relationship between the Increase of Secretion of sTNF_α Induced by Lipopolysaccharides and the Enhanced Expression of TACE mRNA in HL-60 Cells and Adhesive Cells from Human Spleen

After immunoactive cells are stimulated bylipopolysaccharides (LPS) they secretdefined type ofcytokinessuch as IL- 6 ,IL- 1 ,TNFαand so on,whichinvolve in the inflammation response with some certainside- effects[1] . It was discovered that tumor necrosisfactor- α(TNF- α) exists in two types:secreted TNFα(s TNFα) and transmembrane- TNFα(m TNF) with themolecular weight being 1 7ku and 2 6 kurespectively[2 ] . The extra- membrane domain ofm TNFα is cleaved by a certain enzyme an…     

Study on Effects of Extracts from Salvia Miltiorrhiza and Curcurna Longa in Inhibiting Phosphorylated Extracellular Signal Regulated Kinase Expression in Rat＇s Hepatic Stellate Cells

Objective： To study the effect of salvianolic acid B （SAB） and curcumin, the extracts of Solvie Miltiorrhize and Curcume Longe, on the proliferation and activation of hepatic stellate cell （HSC）, and the extracellular signal regulated kinase （ERK） expression in it. Methods： Rat＇s HSC-T6 were cultured and treated by SAB or curcumin. The inhibitory effect on cell proliferation was determined by 3-（4,5-dimthyl-2- 2thiazoly）-2,5-diphenyl-2H-tetrazolium bromide （MTT） colorimetry, and the expression levels of a smooth actin （a-SMA）, collagen type Ⅰ , and ERK were determined by Western blot. Results： SAB and curcumin inhibited the proliferation and activation of rat＇s HSC-T6 in dose-dependent fashion and significantly reduced the expression level of a-SMA （ P〈0.01 ）. Curcumin significantly reduced the expression of collagen type Ⅰ （P〈0.05）. Both SAB and curcumin showed insignificant effect on the ERK expression level, but they could significantly reduce the level of phosphorylated-ERK expression, showing significant difference as compared with that in the control group （ P〈0.01 and P〈0.05 respectively）. Conclusion： SAB and curcumin could significantly inhibit the proliferation, activation of HSC, and the production of type Ⅰ collagen in HSC, the mechanism may be associated with their inhibition on ERK phosphorylation.     

The Effect of Ginkgo Biloba Extract on the Expression of PKCα in the Inflammatory Cells and the Level of IL-5 in Induced Sputum of Asthmatic Patients

To investigate the effect of the Ginkgo Biloba Extract （GBE） on the asthma and examine its possible mechanisms, 75 asthma patients were divided into 4 groups and the patients were respectively treated with fluticasone propionate for 2 weeks or 4 weeks, or treated with fluticasone propionate plus GBE for 2 weeks or 4 weeks. Fifteen healthy volunteers served as healthy controls. Sputum inhalation with inhaling hypertonic saline （4%-5%） was performed. Lung ventilatory function and forced expiratory volume in one second （FEVI） were measured. The numbers of different cells in induced sputum were calculated. The expression of PKCα in the cells was immunocytochemically detected and the percentages of positive cells in different cells were counted. Interleukin-5 （IL-5） in sputum supernatants was detected with enzyme-linked immunosorbent assay. The percentage of eosinophils, lymphocytes, PKCα positive inflammatory cells and the concentration of IL-5 in asthmatic patients were higher than those in the controls （P〈0.05）, and the eosinophils, lymphocytes, positive expression of PKCα and the level of IL-5 were significantly decreased in asthmatic patients after they were treated with fluticasone propionate or fluticasone propionate plus GBE. However, they were still significantly higher than those of the controls. Compared to the group treated with glucocorticosteroid for 2 weeks, no significant decrease was found in the percentage of eosinophils, lymphocytes, PKCα positive inflammatory cells and the IL-5 in the supernatant of induced sputum. Compared with the group treated with glucocorticosteroid for 2 or 4 weeks, significant decrease in the same parameters was observed in the group treated with fluticasone propionate and GBE for 4 weeks. The IL-5 level in the supernatant of induced sputum was positively correlated with the percentage of PKCα-positive inflammatory cells and the percentage of eosinophils in the induced sputum in asthma patient groups respectively （n=150, r=0.83, P〈0.01; n=150,