女性泌尿生殖道沙眼衣原体感染临床分析

目的探讨女性泌尿生殖道沙眼衣原体感染的病因诊断。方法2006年1月至2009年1月对本院妇产科女性泌尿生殖道沙眼衣原体感染患者进行临床分析,病因探讨,治疗观察。结果96例患者治愈,7例无效继续治疗。结论女性泌尿生殖道沙眼衣原体感染的病因主要为不洁性行为,要根治的重要方法是配偶双方治疗。     

Hep-2细胞培养法检测泌尿生殖道沙眼衣原体

沙眼衣原体 (ＣＴ) ,是性传播疾病和眼结膜炎的主要病原微生物。在西方国家 ,5 0 %以上的非淋球菌性尿道炎 (ＮＣＵ)和脓性宫颈炎由ＣＴ引起。它也是盆腔炎、不育症的致病菌[1] 。为了摸索一种可靠而较易实用的检测方法 ,我们对 83例疑为泌尿生殖道沙眼衣原体感染的病人 ,男性取其尿道分泌物 ,女性取其阴道分泌物 ,用Ｈｅｐ 2细胞培养法和ＰＣＲ法进行检测 ,现将结果报道如下。1　材料与方法1 1　材料ＲＰＭＩ16 40 (ＬＩＦＥＴＥＣＨＮＯＬＧＩＥＳ ,ＩＮＣＵＳＡ)按说明书配制 ,加庆大霉素 10 0ＩＵ ｍｌ,Ｇ6滤器过滤除菌 ,加小牛血清 (成…     

血清中沙眼衣原体抗体检测对泌尿生殖道感染的诊断价值

临床上所见的尿道口发红、不适、有粘性分泌物及出现尿频、尿痛的患者 ,在其他常规检查均无明显异常的情况下 ,给临床医师的诊断带来一定困难。本组用免疫金标准方法对上述疑难病例进行了血清中沙眼衣原体抗体的检测和分析 ,以进一步阐明其对泌尿生殖道感染的诊断价值。1　资料与方法1 1　材料　病例来源 :试验组与阳性对照组病例均来源于我院性病门诊患者 ,男 6 2例 ,女 18例 ,年龄 2 1～5 2岁 ,其中以 2 3～ 4 2岁多见。所有患者临床除外眼部沙眼衣原体感染。试验分组 :试验组为本人有临床症状 ,除沙眼衣原体抗体检查外 ,其它各项指标均为…     

一步法免疫层析法检测泌尿生殖道沙眼衣原体感染61例

目的　探讨沙眼衣原体的检验方法。方法　采用美国 ABI公司的沙眼衣原体抗原检测免疫层析法。结果　对 6 1例 (男 37例 ,女 2 4例 )非淋菌性尿道炎的患者尿道分泌物检测 ,有 2 3例沙眼衣原体阳性 ,阳性率达37.7% ,其中男性 15例 ,阳性率 40 .5 % ,女性 8例 ,阳性率 33.37% ,男女之比差异有显著性 (χ2 =0 .32 ,P<0 .0 5 ) ,同国内其它有关沙眼衣原体的报道一致。结论　 Surestep免疫层析法检测沙眼衣原体不仅具有快速、准确、特异性高等特点 ,而且操作简便、无需特殊设备 ,值得在临床加以推广。     

乳糖酸阿奇霉素治疗泌尿生殖道沙眼衣原体感染的疗效

目的探讨乳糖酸阿奇霉素治疗泌尿生殖道沙眼衣原体感染的临床疗效。方法我院在2010年2月至2012年7月期间收治的148例泌尿生殖道沙眼衣原体感染的患者,随机分为治疗组和对照组两组,每组74例,分别行乳糖酸阿奇霉素静脉滴注和强力霉素盐酸盐静脉滴注,密切观察两组患者的临床疗效。结果乳糖酸阿奇霉素治疗泌尿生殖道沙眼衣原体感染的痊愈率(67.57%)和总有效率(90.54%)均显著高于强力霉素对照组的痊愈率(48.65%)和总有效率(70.27%),对泌尿生殖道沙眼衣原体感染有较好的疗效,且未出现严重不良反应。结论乳糖酸阿奇霉素可作为安全有效治疗泌尿生殖道沙眼衣原体感染的首选药物。     

连接酶链反应检测沙眼衣原体诊断泌尿生殖系感染

目的：研究应用连接酶链反应方法（LCR）检测沙眼衣原体（CT）在诊断泌尿生殖道感染中的临床意义。方法：应用LCR对4024份患的尿标本进行CT的检测，其中有47份尿道拭子或宫颈拭子同时进行培养法和LCR的检测，并对两种方法的检测结果进行了比较分析，结果：LCR法检测4024份尿标本，CT阳性率为17．50％（704／4024），417份拭子标本LCR检测阳性率为20．62％（86／417），培养法检测阳性率为8．39％（35／417），LCR法与培养法之间差异有极显性（P＜0．005），结论：LCR法与培养法比较具有敏感，快速和特异的特点，用于检测拭子标本和尿标本在检测CT诊断泌尿生殖道感染中有着重要的意义。     

沙眼衣原体与泌尿生殖系感染

沙眼衣原体与泌尿生殖系感染昆明医学院第二属医院泌尿外科（６５０１０１）杨德林，王剑松综述徐鸿毅审校近１５年来，沙眼衣原体（ＣｈｌａｍｙｄｉａＴｒａｃｈ－ｏｍａｔｉｓ，简称ＣＴ）已被认为是性传播疾病（ＳＴＤ）的一个重要病原体［１］。由ＣＴ引起的泌尿生殖...     

女性生殖道沙眼衣原体的检测与治疗

近年来国外对泌尿生殖造沙眼衣原体的研究较多，其结果表明沙眼衣原体可引起宫颈炎、输卵管炎、盆腔炎而导致不孕或异位妊娠，直接或间接影响生育功能与身体健康，已引起国内、外学者的重视。本文检测100例，报告如下。临床资料一、检测对象：收集1996年期间，来我院妇科门诊就诊的患者100例，常规作宫颈刮片，检测沙眼衣原体、同时作霉菌、滴虫检查。100例患者中，年龄最小22岁、最大58岁，平均年龄324岁。均为已婚妇女，主诉以白带多、外阴搔痒者58例。二、检测方法：按常规作宫颈刮片，固定于95％酒精内。15～20分钟后取出干燥，用1％冰…     

泌尿生殖道衣原体局部抗体的检测

目前衣原体被认为是常见的性病病原体 ,据国外报道5 0 %非淋菌性尿道炎以及 6 6 %急性附睾炎均由衣原体感染所致。本文采用 1983年在国内首次建立衣原体间接血凝试验 ,检测了 788例泌尿生殖道衣原体局部抗体 ,现报道如下。1　材料与方法1.1　标本来源男性患者组 2 14例 ,年龄 2     

In vitro antimicrobial susceptibilities of oxacillin-resistant coagulase negative staphylococci

Minimum inhibitory (broth microdilution) and bactericidal concentrations determined on 101 oxacillin-resistant coagulase-negative staphylococci against netilmicin, oxacillin, vancomycin, Lauricidin and minoxidil. All isolates were susceptible to vancomycin. Netilmicin was the next most active agent, inhibiting 90% of the strains at 8 mcg/ml.     

In vitro activity of GAR-936 against Chlamydia pneumoniae and Chlamydia trachomatis

We evaluated the in vitro activity of GAR-936, a novel glycylcycline antibiotic, against ten isolates of Chlamydia pneumoniae and five strains of C. trachomatis. Susceptibility testing was done in HEp-2 cells. The MIC90s and MBC90s of GAR-936, doxycycline, ofloxacin and clarithromycin against C. pneumoniae were 0.125, 0.25, 0.25 and 0.06 mg/l, respectively. The MICs and MBCs of GAR-936, doxycycline, ofloxacin and clarithromycin against C. trachomatis were 0.03-0.125, 0.25, 0.25-0.5 and 0.06 mg/l, respectively. GAR-936 had excellent activity against both chlamydial species and may have a potential role in the treatment of human chlamydial infection.     

In vitro susceptibility of Pseudomonas maltophilia to antimicrobial agents

Reports on the isolation and pathogenicity of some species of Gram-negative and glucose-nonfermentative bacilli have appeared in the literature since 1962. The infections caused by those organisms have become important problems as an opportunistic infection in immunosuppressed patients. Pseudomonas maltophilia is a glucose-nonfermentative aerobic Gram-negative rod widely dispersed in nature. The role of Pseudomonas maltophilia as an opportunistic pathogen is not thoroughly established, but a few papers on clinical infections associated with P. maltophilia have been published. P. maltophilia is considerably less susceptible to certain of the most commonly-used antibiotics. We have reviewed the susceptibilities of P. maltophilia to antimicrobial agents currently available and yearly changes of the strains in resistance to the antibiotics that have been extensively used.     

Clinical studies on doxycycline treatment of urogenital Chlamydia trachomatis infections

Nineteen patients of male NGU and 8 patients of female NGC were treated with doxycycline (DOXY, 200 mg/day for 14 days). C. trachomatis positive rate of male NGU was 12/19 (63%), and that of female NGC was 4/8 (50%). C. trachomatis positive rate of male NGU after treatment of DOXY for 3 days, 7 days and 14 days, was 83%, 17% and 0%, respectively. Serous discharge after treatment of DOXY for 3 days, 7 days, 14 days, was continued in 100%, 33%, 0%. Administration of DOXY (200 mg/day for 14 days) was considered good treatment for male C. trachomatis positive NGU.     

In vitro activity of antimicrobial agents against mycobacteria

The aims of this study were to investigate the possible effects of new antimicrobial agents, the conventional antituberculosis drugs and several combinations of these agents against 190 clinical isolates of Mycobacterium tuberculosis and 30 of M. avium.     

In vitro antimicrobial susceptibilities of 349 methicillin-resistant Staphylococcus aureus isolates from veterans

Three hundred and forty-nine methicillin resistant Staphylococcus aureus (MRSA) isolates from veterans were tested (by disc agar diffusion) for their in vitro activity against 18 antimicrobial agents. At least 90% of the isolates were susceptible to bacitracin, nitrofurantoin, hydrogen peroxide, novobiocin, netilmicin and vancomycin. We feel that the aminoglycoside, netilmicin, might provide an alternative agent (to intravenously administered vancomycin) for treating multiply-antimicrobial resistant MRSA. In addition, hydrogen peroxide exhibited very good activity against the test isolates and may have some use as a topical agent for reduction of MRSA on skin and some mucous membranes. This study suggests that further evaluation of netilmicin and hydrogen peroxide (topical only) might be useful.     

In vitro activity of antimicrobial agents against Acinetobacter calcoaceticus

The aim of this study was to evaluate the in vitro activity of several new microbial agents against 96 Acinetobacter calcoaceticus isolates. Among several new beta-lactams, imipenem, a new, broad-spectrum, highly potent penem, was the most active drug in vitro against these strains, with a geometric mean MIC of about 0.3 mg/l. Ceftazidime and ceftizoxime also demonstrated good in vitro activity with geometric mean MICs of 6 mg/l and 7 mg/l respectively. Among new quinolones, ciprofloxacin, ofloxacin and pefloxacin were substantially active in vitro: geometric means were 0.8, 0.9 and 1.5 mg/l respectively. A progressive increase in resistance to aminoglycosides has been observed and 80 to 90% of isolates were resistant to all but amikacin, tobramycin and habekacin, which showed geometric mean MICs of 7.0, 6.0 and 1.2 mg/l.     

In vitro susceptibility of four serotypes of enterohaemorrhagic Escherichia coli to antimicrobial agents

We evaluated the in vitro susceptibility of four serotypes of enterohaemorrhagic Escherichia coli (E. coli 026, E. coli O111, E. coli O157, and E. coli O165) with diverse DNA patterns to antimicrobial agents. The minimum inhibitory concentrations (MIC) determined in a total of 83 strains using Mueller-Hinton agar under aerobic and anaerobic conditions were 0.015-0.12 microg/ml for ciprofloxacin, 0.06-1 microg/ml for norfloxacin, 2-64 microg/ml for fosfomycin without glucose-6-phosphate (G-6-P), 0.25-32 microg/ml for fosfomycin with G-6-P, 2- > or = 256 microg/ml for kanamycin, 0.125-2 microg/ml for cefoperazone, and 0.06-1 microg/ml for ceftazidime. The MIC of ciprofloxacin, norfloxacin, cefoperazone, and ceftazidime were low in all strains examined.