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1.
Background Micro RNAs have recently been considered as biomarkers in several different cardiovascular diseases,however,so far there are no circulating miRNAs data about hypertension.Therefore,the aim of the present pilot study was to identify circulating miRNAs for hypertension biomarkers.Methods Using an Agilent microarray,plasma miRNAs were profiled from plasma samples of 10 patients with untreated essential hypertension and 10 healthy controls.Candidate biomarkers identified in the profiles were subjected to validation by using quantitative PCR in an independent sample set of 20 patients with untreated essential hypertension and 20 healthy controls.Then,we assessed the selected miRNAs for the detection and diagnosis of hypertension from plasma samples of 70 patients with untreated essential hypertension and 20 healthy controls.The Spearman correlation coefficient was used to assessed the selected miRNAs correlations with blood pressure.The area under the receiver operating characteristic curve(AUC) was used to evaluate diagnostic accuracy.Results The expressions of selected 8 miRNAs were investigated independently in plasma samples from 10 hypertension patients and 10 healthy subjects.The levels of circulating miR-30c-5p,miR-133 b,miR-29b-3p,miR-29a-3p,miR-29c-3p,miR-30a-3p,miR-let7b-3p expression were significantly down regulated in hypertension group compared with healthy group and the level of hsa-miR-92b-3p was significantly unregulated between the groups.We used q RT-PCR assay to confirm the expression of 8 candidate miRNAs,miR-30c-5p(P 0.001),miR-29b-3p(P 0.001),miR-29a-3p(P = 0.027),miR-29c-3p(P 0.001),miR-92b-3p(P = 0.003),miR-30a-3p(P = 0.704),miR-133b(P =0.346),andmiR-let7b(P = 0.161).The diagnostic accuracy of miR-30c-5p,miR-29b-3p,miR-29a-3p,miR-29c-3p and miR-92b-3p,as measured by AUC,were 0.897,0.90,0.829,0.825 and 0.832,respectively,with all P 0.001.Conclusions The plasma levels of miR-30c-5p,miR-29b-3p,miR-29a-3p,miR-29c-3p and miR-92b-3p associated with hypertension which provide an impetus for future evaluations of hypertension development,progression,therapeutic efficacy and prognosis.  相似文献   

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目的探讨结核潜伏感染者全血miR-144-3p、miR-146a-5p的表达及其与活动性肺结核患者的差异。方法收集结核潜伏感染者30例,活动性肺结核患者30例。收集全血,提取总RNA,利用反转录-荧光定量PCR方法检测miR-144-3p、miR-146a-5p的表达。两组间比较采用t检验。结果结核潜伏感染者全血中miRNA144-3p的表达(2.014±1.48)比活动性肺结核患者(1.056±0.746)明显提高,差异有统计学意义(P<0.05),而结核潜伏感染者全血中miR-146a-5p的表达(1.937±1.109)与活动性肺结核患者(1.469±0.693)相似,差异无统计学意义(P>0.05)。结论 miR-144-3p可能成为诊断结核潜伏感染者的标志物。  相似文献   

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Background:To investigate the relationship between the expression level of hsa-miR-34a-5p and liver injury and to further explore its regulatory signaling pathwaysMethods:Liver tissue and blood were collected from 60 patients undergoing hepatectomy. We constructed a rat HIRI model and treated it with an intraperitoneal injection of agomir-miR-34a-5p or agomir-normal control (NC) for 7 days after the surgery. The pathological changes of agomir-miR-34a-5p or agomir-normal control (NC) groups were compared. 7702 and AML12 cells were transfected with mimics NC or miR-34a-5p mimics and then treated with H2O2 for 6 hours. Cell apoptosis was detected by flow cytometry, Western blot, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, respectively. Furthermore, the target genes of miR-34a-5p were identified by luciferase reporter gene assay and were verified in vitro.Results:The relatively high miR-34a-5p expression group revealed a lower level of alanine aminotransferase and aspartate aminotransferase compared with the relatively low miR-34a-5p expression group. HIRI+agomir-miR-34a-5p rats exhibited significantly higher miR-34a-5p expression, lower serum alanine aminotransferase, aspartate aminotransferase, alleviated hepatic necrosis, reduced hepatocyte apoptosis, and decreased expression of apoptosis-related proteins, when compared with HIRI+agomir-NC rats (P < .05). After hydrogen peroxide treatment, alpha mouse liver-12 cell (AML-12) and normal liver cell line LO2 (LO2) cells transfected with miR-34a-5p mimics had significantly lower apoptosis rate compared with miR-34a-5p mimics NC group (P < .05). Hepatocyte nuclear factor 4α was identified as a miR-34a-5p target gene. Hepatocyte nuclear factor 4α expression was significantly downregulated in AML12 and HL-7702 (7702) cells transfected with miR-34a-5p (P < .05). Moreover, AML12 and 7702 cells transfected with miR-34a-5p significantly showed higher c-Jun N-terminal kinase (JNK), P38, cleavage cas-3, and BCL2 associated X (Bax) protein levels compared with AML12 and 7702 cells transfected with agomir-NC.Conclusion:miR-34a-5p possibly protected the liver from I/R injury through downregulating Hepatocyte nuclear factor 4α to inhibit the JNK/P38 signaling pathway.  相似文献   

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BackgroundAtrial fibrillation (AF) is a type of cardiac arrhythmia which is caused by irregular electrical activities in the atria.ObjectiveTo identify serum microRNA (miRNA) biomarkers at three durations (duration since diagnosis of AF) of AF.MethodsThis study included 14 patients with AF and 8 healthy subjects. The blood sample was collected from each patient at baseline (time of diagnosis) and 12-month and 24-month follow-up periods. The serum was used for miRNA sequencing. The differentially expressed miRNAs (DEMs) between the 3 AF and control groups were independently compared. The predicted target genes of DEMs were subjected to functional enrichment and protein-protein interaction network analyses. Additionally, the miRNA-target gene networks were constructed for the 3 AF groups and miRNA time series analysis was performed. The expression of several key miRNAs was verified by real-time quantitative polymerase chain reaction (qRT-PCR).ResultsIn total, 28, 22, and 24 DEMs were identified in the baseline, 12-month, and 24-month groups, respectively. miR-483-5p was the common DEM in the 3 AF groups. In the baseline and 12-month groups, the miR-200b-3p and miR-125b-5p target genes were significantly enriched in the Wnt signaling and several cancer-related pathways, respectively. In the 12-month group, the miR-34a-5p target genes were enriched in the cancer-related pathways. In the miRNA-target gene network, miR-34a-5p regulated the highest number of target genes. The time series analysis revealed that 7 miRNAs, which were downregulated in the control group, were upregulated in the AF groups. The qRT-PCR analysis revealed that the 24-month group exhibited a significant upregulation of miR-483-5p (p < 0.05), whereas the baseline group exhibited significant a downregulation of miR-125b-5p (p < 0.05).ConclusionIn patients with AF, miR-125b-5p and miR-483-5p can be potential biomarkers of the baseline and 24-month periods, respectively.  相似文献   

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Background: Increasing evidence shows that microRNA-7a-5p (miR-7a-5p) plays an important role in regulating the inflammatory process in inflammatory bowel disease (IBD). How miR-7a-5p contributes to this process is poorly defined. The purpose of this study was to examine whether miR-7a-5p regulates 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced inflammatory responses via the JNK pathway.MethodsColitis was induced in male mice by intracolonic administration of TNBS; mice were divided into 3 groups: normal control (NC), TNBS, and miR-7a-5p antagomir-treated group. Inflammatory responses were estimated by disease activity index (DAI) and histological scores. The relative expressions of miR-7a-5p and tight junction protein, ZO-1, were detected by RT-qPCR. Western blot assays were used to estimate the level of JNK pathway proteins and ZO-1. After miRNA-antagomir injection, the extent of colonic tissue injury and expression levels of ZO-1 and JNK in intestinal tissue were compared.ResultsmiR-7a-5p and p-JNK expression were higher in the intestinal tissue of the TNBS group as compared to NC. Inhibition of the expression of miR-7a-5p resulted in significantly decreased expression of p-JNK but increased expression of ZO-1 and promoted the recovery of intestinal mucosa.ConclusionThis work demonstrates a correlation between the JNK pathway and miR-7a-5p in TNBS-induced experimental colitis in mice, which may provide a new research direction for the treatment of IBD.  相似文献   

6.
Objective

Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate this, a set of well-characterized RA families originating from northern Sweden were analyzed for differential expression of a selected set of microRNAs.

Method

MicroRNA was isolated from frozen peripheral blood cells obtained from 21 different families and included 26 RA patients, 22 FDRs, and 21 HCs. Expression of the selected microRNAs miR-22-3p, miR-26b-5p, miR-34a-3p, miR-103a-3p, miR-142-3p, miR-146a-5p, miR-155, miR-346, and miR-451a was determined by a two-step quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis including clinical variables was applied.

Results

Out of the nine selected microRNAs that previously have been linked to RA, we confirmed four after adjusting for age and gender, i.e., miR-22-3p (p?=?0.020), miR-26b-5p (p?=?0.018), miR-142-3p (p?=?0.005), and miR-155 (p?=?0.033). Moreover, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. In addition, analysis of the effect of corticosteroid use showed modulation of miR-103a-3p expression.

Conclusions

We confirm that microRNAs seem to be involved in the development of RA, and that the expression pattern in FDR is partly overlapping with RA patients. The contribution of single microRNAs in relation to the complex network including all microRNAs and other molecules is still to be revealed.

Key Points
? Expression levels of miR-22-3p, miR-26b-5p, miR-142-3p, and miR-155 were significantly altered in RA patients compared to those in controls.
? In first-degree relatives, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs.
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目的 分析血清微小核糖核酸-92a-1-5p ( miR-92a-1-5p)、 miR-92a-2-5p 表达水平与老年卒中后抑郁的关系.方法 选取2018年2月至2020年10月胶州中心医院收治的129例老年卒中患者为研究组,另选取110名同期健康体检者为对照组,均检测血清miR-92a-1-5p、miR-92a-2...  相似文献   

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目的探讨达格列净对2型糖尿病(T2DM)早期糖尿病肾病(DKD)患者尿外泌体微RNA(miRNA)的影响。方法选取2018年9月至2019年9月于徐州医科大学附属淮安医院内分泌科住院的T2DM早期DKD患者60例。将纳入的患者按照尿微量白蛋白与尿肌酐比值(UACR)进行分组,其中30 mg/g1c)及血脂等生化指标。计算体质指数(BMI)。运用实时聚合酶链反应、芯片法测定尿外泌体miRNA水平。组间比较采用t检验、单因素方差分析、χ2检验、Wilcoxon秩和检验或Mann-Whitney U检验。结果DAP组患者治疗后与治疗前相比,体重、BMI、FPG及餐后2 h血糖水平均降低,差异有统计学意义(P<0.05)。DKD组患者治疗后与治疗前相比,体重、BMI、HbA1c、FPG、餐后2 h血糖及UACR水平均降低,差异有统计学意义(P<0.05)。与对照组相比,传统药物治疗组中5种miRNA(miR-let-7b-5p、miR-21-5p、miR-182-5p、miR-200c-3p和miR-423-5p)表达上调,差异具有统计学意义(P<0.05);DKD组与传统药物治疗组相比,上述5种miRNA表达亦上调,差异具有统计学意义(P<0.05)。DKD组治疗后上述5种miRNA的表达较治疗前明显降低,差异有统计学意义(P<0.05)。结论T2DM早期DKD患者存在多种尿外泌体miRNA表达的失调,达格列净可下调细胞外泌体miRNA的表达。  相似文献   

11.
The shift of the tumour immune microenvironment to a suppressive state promotes not only the development and progression of the disease in multiple myeloma (MM) but also the development of resistance to immunotherapy. We previously demonstrated that myeloma cells can induce monocytic myeloid-derived suppressor cells (M-MDSCs) from healthy peripheral blood mononuclear cells (PBMCs) via the concomitant secretion of CC motif chemokine ligand 5 (CCL5) and macrophage migration inhibitory factor (MIF), but an unknown mediator also promotes M-MDSC induction. This study demonstrates that miR-106a-5p and miR-146a-5p delivered by tumour-derived exosomes (TEXs) from myeloma cells play essential roles in M-MDSC induction in MM. MiR-106a-5p and miR-146a-5p upregulate various immunosuppressive/inflammatory molecules in PBMCs, such as IDO1, CD38, programmed death-ligand 1, CCL5 or MYD88, which are involved in interferon (IFN)-α response, IFN-γ response, inflammatory response, tumour necrosis factor-α signalling and Interleukin-6-JAK–STAT3 signalling. These molecular features mirror the increases in myeloid cellular compartments of PBMCs when co-cultured with myeloma cells. MiR-106a-5p and miR-146a-5p have a compensatory relationship, and these two miRNAs collaborate with CCL5 and MIF to promote M-MDSC induction. Collectively, novel therapeutic candidates may be involved in TEX-mediated sequential cellular and molecular events underlying M-MDSC induction, potentially improving the efficacy of immunotherapy.  相似文献   

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目的探讨相关microRNA(miRNA)与冠心病氯吡格雷低反应的相关性及其作为生物标志物的诊断价值。方法筛选158例稳定型冠心病患者作为研究对象,采用光比浊法检测二磷酸腺苷(ADP)诱导的最大血小板聚集率(MPAR),将其中MPAR≥65%的35例患者纳入氯吡格雷低反应组(低反应组),并按年龄、性别配对将35例MPAR<65%的患者纳入氯吡格雷正常反应组(正常反应组)。采集患者外周血单核细胞并提取总RNA,利用实时荧光定量PCR技术检测miR-34a-5p、miR-370-3p、miR-432-5p和miR-495-3p在两组患者中的表达差异,并通过相关性分析和受试者工作特征(ROC)曲线分析评估差异miRNA对氯吡格雷低反应的诊断价值。结果与正常反应组相比,低反应组患者的miR-34a-5p和miR-495-3p表达显著升高,且差异倍数大于2(P<0.05);而miR-370-3p和miR-432-5p的表达差异不显著(P>0.05)。miR-34a-5p和miR-495-3p的表达与冠心病患者MPAR均呈显著正相关(r=0.709,P<0.01;r=0.5...  相似文献   

15.
Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus, responsible for an atypical pneumonia that can progress to acute lung injury. MicroRNAs are small non-coding RNAs that control specific genes and pathways. This study evaluated the association between circulating miRNAs and lung injury associated with COVID-19. Methods: We evaluated lung injury by computed tomography at hospital admission and discharge and the serum expression of 754 miRNAs using the TaqMan OpenArray after hospital discharge in 27 patients with COVID-19. In addition, miR-150-3p was validated by qRT-PCR on serum samples collected at admission and after hospital discharge. Results: OpenArray analysis revealed that seven miRNAs were differentially expressed between groups of patients without radiological lung improvement compared to those with lung improvement at hospital discharge, with three miRNAs being upregulated (miR-548c-3p, miR-212-3p, and miR-548a-3p) and four downregulated (miR-191-5p, miR-151a-3p, miR-92a-3p, and miR-150-3p). Bioinformatics analysis revealed that five of these miRNAs had binding sites in the SARS-CoV-2 genome. Validation of miR-150-3p by qRT-PCR confirmed the OpenArray results. Conclusions: The present study shows the potential association between the serum expression of seven miRNAs and lung injury in patients with COVID-19. Furthermore, increased expression of miR-150 was associated with pulmonary improvement at hospital discharge.  相似文献   

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Background:Reported studies have shown that expression levels of microRNAs (miRNAs) are related to survival time of patients with heart failure (HF). A systematic review and meta-analysis were conducted to study circulating miRNAs expression and patient outcome.Methods:Meta-analysis estimating expression levels of circulating miRNAs in HF patients from January 2010 until June 30, 2018, through conducting online searches in Pub Med, Cochrane Database of Systematic, EMBASE and Web of Science and reviewed by 2 independent researchers. Using pooled hazard ratio with a 95% confidence interval to assess the correlation between miRNAs expression levels and overall survival.Results:Four relevant articles assessing 19 circulating miRNAs in 867 patients were included. In conclusion, the meta-analysis results suggest that HF patients with low expression of serum miR-1, miR-423-5p, miR-126, miR-21, miR-23, miR-30d, miR-18a-5p, miR-16-5p, miR-18b-5p, miR-27a-3p, miR-26b-5p, miR-30e-5p, miR-106a-5p, miR-233-3P, miR-301a-3p, miR-423-3P, and miR-128 have significantly worse overall survival (P<.05). Among them, miR-18a-5p, miR-18b-5p, miR-30d, miR-30e-5p, and miR-423-5p are strong biomarkers of prognosis in HF.  相似文献   

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目的 探讨miR-302b-3p靶向丝/苏氨酸蛋白激酶(AKT)1调节皮肤成纤维细胞衰老的作用及其分子机制.方法 建立复制性细胞衰老模型后,采用real-time PCR法检测细胞miR-302b-3p的表达情况;采用生物信息学软件分析miR-302b-3p的靶基因;分别将miR-302b-3p模拟物(mimic)及抑制剂(inhibitor)转染皮肤成纤维细胞后,β-半乳糖苷酶染色分析细胞衰老情况,qRT-PCR法检测AKT1 mRNA表达水平,Western印迹法检测AKT1蛋白表达情况.结果 与对照组比较,复制性衰老皮肤成纤维细胞中miR-302b-3p显著升高.转染miR-302b-3p mimic后,细胞衰老阳性染色细胞数目显著增加(P<0.01).AKT1为miR-302b-3p的预测靶基因.当细胞上调miR-302b-3p表达时,靶基因AKT1 mRNA及蛋白水平显著降低(P<0.05).反之,当细胞下调miR-302b-3p表达时,靶基因AKT1 mRNA及蛋白水平显著升高(P<0.05).结论 miR-302b-3p可能通过靶向抑制AKT1表达调节皮肤成纤维细胞的衰老.  相似文献   

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目的 探讨大黄酸(Rhein)对人胃癌细胞(SGC-7901)增殖、迁移、侵袭、凋亡的影响及其机制.方法 通过qRT-PCR检测大黄酸处理后细胞中miR-29c-3p的表达以及miR-29c-3p的转染效率;miR-29c-3p mimics组、NC mimics组、Rhein+miR-29c-3p inhibitor...  相似文献   

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ObjectiveExplorations have been progressing in decoding the mechanism of non-small cell lung cancer (NSCLC). However, long noncoding RNA small nucleolar RNA host gene 5/microRNA-181c-5p/chromobox protein 4 (SNHG5/miR-181c-5p/CBX4) axis-oriented mechanisms in NSCLC is still in infancy. Therein, this study is proposed to probe this axis in NSCLC progression.MethodsSamples of 86 NSCLC patients were collected and SNHG5, miR-181c-5p and CBX4 expression was detected in NSCLC tissues and cells. NSCLC cells were transfected with plasmids to change SNHG5, miR-181c-5p or CBX4 expression, after which cell functions and phosphorylated (p)-nuclear factor (NF)-κB protein expression were evaluated. The relationships among SNHG5, miR-181c-5p and CBX4 were validated. Tumor xenografts were implemented to verify the roles of SNHG5, miR-181c-5p and CBX4 in tumor growth.ResultsLow miR-181c-5p and high SNHG5 and CBX4 levels were found in NSCLC tissues and cells. Restoration of miR-181c-5p or knockdown of SNHG5 or CBX4 restrained NSCLC cell progression and inactivated the NF-κB pathway. Upregulated CBX4 abolished the effects of miR-181c-5p on reducing NSCLC cell progression. SNHG5 regulated the interaction between miR-181c-5p and CBX4. In vivo, restoration of miR-181c-5p or knockdown of SNHG5 or CBX4 retarded the tumor growth.ConclusionThis study has delineated that SNHG5 induces the NF-κB pathway by regulating the miR-181c-5p/CBX4 axis to promote NSCLC progression, which may pave a novel path for NSCLC treatment.  相似文献   

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摘要 目的:探讨急性脑梗死(ACI)患者血浆中微小RNA(miR)-181a-5p的表达水平及临床价值。方法:收集148例ACI患者(ACI组)的临床资料,根据美国国立卫生研究院卒中量表(NIHSS)评分将其分为:轻度72例、中度35例、重度41例。ACI患者发病4.5h内用阿替普酶静脉溶栓治疗。根据出院后第90天的预后情况,将患者分为预后良好组(102例)和预后不良组(46例)。另选取同期健康体检者100例为对照组。用逆转录-聚合酶链反应(RT-PCR)检测血浆miR-181a-5p表达水平;酶联免疫吸附试验(ELISA)检测血浆中凋亡分子B淋巴细胞瘤-2基因(Bcl-2)和含半胱氨酸的天冬氨酸蛋白水解酶(caspase-1)表达水平。相关性检验用Pearson分析;用Logistic多因素回归分析影响预后的因素;绘制受试者工作特征(ROC)曲线,分析血浆miR-181a-5p预测预后的价值,计算曲线下面积(AUC)、灵敏度及特异性。结果:与对照组比较,ACI组血浆miR-181a-5p和caspase-1表达水平较高,而Bcl-2表达水平较低(P均<0.05)。miR-181a-5p与caspase-1呈正相关(r=0.176,P=0.032),与Bcl-2呈负相关(r=-0.209,P=0.011)。轻度、中度和重度ACI患者血浆miR-181a-5p表达水平分别为(1.35±0.32)、(1.79±0.20)、(2.34±0.59),经单因素方差分析,组间比较差异有统计学意义(F=32.001,P<0.001),病情越重,血浆miR-181a-5p表达水平越高。与预后良好组比较,预后不良组年龄更大,入院时NIHSS评分、血浆miR-181a-5p和caspase-1水平较高,而Bcl-2水平较低(P均<0.05)。多因素分析显示,年龄、入院时NIHSS评分及血浆miR-181a-5p水平是ACI患者预后不良的独立影响因素(OR=1.532、1.832、3.111,P均<0.05)。血浆miR-181a-5p预测不良预后的AUC为0.856(95%CI:0.815~0.886,P<0.01),灵敏度为89.56%,特异性为70.12%。结论:ACI患者血浆中miR-181a-5p表达水平明显升高,对ACI病情和预后的早期评估有一定价值。  相似文献   

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