E-钙黏素在鳞癌、Bowen病和脂溢性角化病中的表达

目的: 检测E-钙黏素(E-cadherin)在皮肤鳞状细胞癌(SCC)、Bowen病(BD)、脂溢性角化病(SK)中的表达.方法: 用免疫组化染色方法检测10例正常皮肤,20例SCC、20例BD、20例SK中E-钙黏素的表达.结果: E-钙黏素在SK表皮中的表达与正常表皮相似,在SCC中表达显著减弱或完全无表达,而在Bowen病表皮正常区域表达正常或下调.结论: E-钙黏素在恶性皮肤癌中表达异常,可能与表皮肿瘤的侵袭和转移有关.     

皮肤肿瘤

971106鳞癌及角化棘皮癌中P53表达的研究/李东霞…∥中国皮肤性病学杂志.1966,10(5).-267采用DO-1单克隆抗体免疫组化方法检测13例鳞癌(SCC)及角化棘皮瘤(KA)中P53的表达.结果SCC的P53标本阳性率为61.5%,P53阳性肿瘤细胞分布于肿瘤组织全层,KA中P53标本阳性率为15.4%,P53阳性细胞散在于肿瘤组织周边部位.13例SCC按病理学分级,其P53标本阳性率分别为1级25.0%,Ⅱ级71.4%,Ⅲ级100%,显示P53阳性率与SCC的分化程度呈负相关.提示突变型P53基因表达可能与SCC恶性程度有关.P53表达对鉴别SCC与KA有一定意义.表2参7(张国平)971107 婴幼儿颜面出血管癌和血管畸形的诊断与手术疗效评价/黄建华…∥湖南医科大学学报.-1996,21(5).-450根据临床生物学行为和内皮细胞特征将其分为血     

Survivin和p53在皮肤癌中的表达及其临床意义

为检测凋亡抑制基因Survivin和p53基因在皮肤鳞状细胞癌(SCC)和基底细胞癌(BCC)中的表达及临床意义.用免疫组织化学SP法对40例BCC,30例SCC,18例脂溢性角化(SK),16例正常皮肤组织石蜡标本进行Survivin和p53蛋白表达的检测和分析.(1)Survivin在BCC、SCC中的表达阳性率分别为70%和80%,明显高于正常皮肤和SK组(P＜0.01).(2)p53在BCC和SCC中的阳性表达率分别为:45%和56.67%,明显高于正常皮肤和SK组(P＜0.01).(3)BCC和SCC中Survivin与p53的表达呈正相关(r=0.373,r=0.404,P＜0.05).凋亡抑制基因Survivin可能参与BCC和SCC的发生发展,Survivin和p53的检测对BCC和SCC的诊断和进一步治疗提供有效的依据.     

RUNX3蛋白在日光性角化病、鲍恩病及鳞状细胞癌中的表达及意义

目的:探讨RUNX3蛋白在日光性角化病(SK)、鲍恩病(BD)及鳞状细胞癌(SCC)组织中的表达及其作用机制和临床意义。方法:采用免疫组化(SP法)对正常皮肤组织(正常对照组)(10例),SK(30例)、BD(30例)及高(30例)、中(30例)、低分化的皮肤SCC(30例)组织分别对RUNX3进行染色,并采用Image-Pro Plus(IPP)6.0图像分析软件定量分析每个视野阳性表达的平均吸光度(A)值。结果:1RUNX3蛋白在正常对照组中平均A值为0.1841±0.0022;SK组为0.1922±0.0025;BD组为0.1926±0.0019;高分化SCC组为0.1943±0.0025,中分化SCC组为0.1945±0.0028,低分化SCC组为0.1946±0.0033。各组RUNX3蛋白的阳性表达差异有统计学意义(P0.05)。2正常对照组分别与SK组、BD组及高、中、低分化SCC组比较,差异均有统计学意义(P均0.05);SK组和BD组分别与高、中、低分化SCC组比较,差异亦均有统计学意义(P均0.05);而SK组与BD组比较,差异无统计学意义(P0.05);高、中、低分化SCC组各组之间两两比较,差异无统计学意义(P0.05)。结论:RUNX3蛋白在SCC中的表达高于BD及SK,正常皮肤低表达或不表达,可以看出RUNX3蛋白在皮肤SCC中有可能参与了肿瘤发生及发展,可能有致瘤的作用;RUNX3是否可能成为皮肤肿瘤早期诊断、鉴别诊断、肿瘤恶性程度分级及预后评估的生物学指标之一,有待进一步研究。     

论著：皮肤良恶性肿瘤中p16、p21^ras和ki-67蛋白表达研究

目的探讨细胞周期依赖性激酶抑制蛋白、癌基因蛋白和细胞增殖指数在皮肤基底细胞癌(BBC)、鳞状细胞癌(SCC)和脂溢性角化病(SK)中的表达,并就其结果进行分析.方法采用DAKO EnVision免疫组化法检测了18例BCC、10例SCC皮肤恶性肿瘤和17例SK皮肤良性肿瘤组织中p16、p21ras及ki-67蛋白的表达.结果BCC肿瘤组织中p16、p21ras及ki-67阳性表达率分别为61.1%、77.8%、94%;SCC肿瘤组织中阳性表达率分别90%、50%、90%;SK组织中阳性表达率分别为70.5%、88.2%、94.1%.BCC中p16阳性率明显低于SCC(P＜0.01);SCC中p21ras阳性率明显低于SK(P＜0.01);三组ki-67蛋白均呈高表达,它们之间无统计学差异.结论p16、p21ras、ki-67基因与肿瘤的发生发展及增殖程度密切相关.p16、p21ras基因蛋白的缺失程度或超表达在临床上可作为对良恶性肿瘤预后判断的参考值,它们的表达可能与肿瘤的分化程度有关.     

皮肤肿瘤

20120310转录因子Oct-4在皮肤鳞状细胞癌、脂溢性角化病的组织表达及其意义/李毓阳(海南农垦三亚医院皮肤科),戴永江…//南方医科大学学报.-2011,31(5).-917 ～918采用免疫组化法检测Oct-4在35例鳞状细胞癌(SCC),21例脂溢性角化病(SK)及15例正常对照皮肤组织中的表达.结果:Oct-4在正常皮肤组织表达为阴性,在SCC和SK表达差异有统计学意义(P＜0.05),间接反映SCC和SK的恶性程度不同.提示SCC和SK中表达Oct-4基因的细胞很可能是SCC和SK的干细胞,这将为其最终分离和鉴定人SCC和SK干细胞提供重要基础,有助于揭示SCC和SK的肿瘤起始细胞,从而对预防和治疗SCC和SK有重大意义.     

皮肤肿瘤

20131596 hTERT在皮肤鳞状细胞癌中的表达/王松芬(山东日照市皮防所),王桂伟,郑成斌…∥中国麻风皮肤病杂志.-2013,29(5).-307~309采用免疫组织化学PV-6000法检测50例皮肤鳞状细胞癌(SCC)、20例Bowen病(BD)、15例日光角化病(DK)及10例正常对照皮肤组织中hTERT蛋白的表达水平。结果:hTERT蛋白的表达在对照组和SK组、BD组、SCC组之间,SK组和SCC组之间,高分化组和中低分化组之间,无淋巴结转移组和有淋巴结转移组之间比较,差异均有统计学意义(P均<0.05)。认为hTERT的异常表达可能与皮肤鳞状细胞癌的发生及演     

Survivin和Ki-67在皮肤鳞癌和基底细胞上皮瘤的表达及相关性分析

目的观察Survivin和Ki-67在皮肤鳞癌(SCC)及基底细胞上皮瘤(BCE)的表达,并探讨其意义。方法采用免疫组化法检测15例皮肤SCC,20例BCE中Survivin和Ki-67的表达情况,并以16份整形手术切除的正常组织作为对照。结果Survivin在皮肤SCC和BCE中的阳性表达率显著高于正常对照组,且在皮肤SCC的阳性表达率也显著高于BCE,差异均有统计学意义(P均<0.05)。Ki-67在皮肤SCC和BCE中的阳性表达也显著高于正常对照组(P<0.05),但在皮肤SCC和BCE组织中的表达比较,差异无统计学意义(P>0.05)。皮肤SCC和BCE组织中Survivin和Ki-67的阳性细胞表达呈正相关(r=0.77,P<0.05)。结论Survivin与Ki-67在皮肤SCC和BCE组织中的表达均升高,且呈正相关,提示两基因有可能共同参与肿瘤的发生和发展。     

突变型P53基因产物在皮肤鳞状细胞癌和基底细胞癌中的表达

应用免疫组化技术检测皮肤鳞状细胞癌(SCC)和基底细胞癌(BCC)中P53基因突变产物。结果显示:正常皮肤粘膜组织中无P53蛋白表达。而在肿瘤标本中,36例SCC中55.6％(20/36),33例BCC 45.5％(15/33)P53蛋白过度表达,两者无显著性差异(χ~2=0.33,P>0.05)。源于肛门——生殖器周围的SCC中67％(8/12)P53免疫组化阳性。P53基因产物可以在肿瘤组织和/或其表皮中表达。结果提示P53基因突变在皮肤恶性肿瘤发生、发展过程中起重要作用,P53免疫组化对皮肤恶性肿瘤有一定诊断价值,但不能鉴别BCC和SCC。SCC组织学分化程度可能与P53基因突变无关。     

组织蛋白酶B,Ki67在皮肤鳞状细胞癌、基底细胞癌和脂溢性角化病的表达

目的观察组织蛋白酶B(CB)、细胞增殖核抗原(Ki67)在皮肤鳞状细胞癌(SCC)、基底细胞癌(BCC)和脂溢性角化病(SK)等组织中的表达,并分析其表达差异的意义。方法用免疫组化SP染色法检测CB,Ki67在15例SCC,15例BCC,14例SK及10例正常对照皮肤组织中的表达。结果 CB在正常皮肤组织表达为阴性,在SK,BCC,SCC瘤组织中表达依次升高,差异有显著性(P0.05);Ki67在SK,BCC和SCC三者表达差异亦有显著性(P0.05)。结论 CB,Ki67在SK,BCC和SCC瘤组织中的阳性表达率依次升高,可能与SCC侵袭和转移有关。     

环氧化酶-2在表皮肿瘤中的表达

目的 探讨环氧化酶-2在不同表皮肿瘤中的表达及意义。方法 选择鳞状细胞癌8例、基底细胞上皮瘤10例、Bowen病8例和脂溢性角化病12例,运用免疫组化方法观察肿瘤细胞中环氧化酶-2的表达。结果 与正常表皮相比,环氧化酶-2在鳞状细胞癌、Bowen病、基底细胞上皮瘤中的表达明显上调,尤其以鳞状细胞癌中的表达最强。而环氧化酶-2在脂溢性角化病中的表达与正常人皮肤的表达近似。结论 环氧化酶-2表达的上调可能在表皮肿瘤的发生发展中发挥一定作用。     

Immunohistochemical expression of cyclooxygenase-2 in skin cancers

BACKGROUND: Cyclooxygenase (COX), also known as prostaglandin endoperoxide synthase, catalyses the conversion of arachidonic acid to prostanoids. There are two different isoforms of COX, referred to as COX-1 and COX-2. Overexpression of COX-2 has been demonstrated in various neoplasms, such as experimentally promoted tumors, gastrointestinal cancers and breast tumors. METHODS: In this study, we used immunohistochemistry to investigate COX-2 expression in a series of basal cell epitheliomas (BCE), Bowen's disease, squamous cell carcinomas (SCC) and metastatic tumors of the skin. RESULTS: Four of 16 BCE showed a positive reaction for COX-2 and the adenoid type of BCE was the most strongly positive. In Bowen's disease, the extent of positive staining for COX-2 was even higher than that in BCE. Eleven of 15 SCC showed a positive reaction for COX-2 and the pattern of staining was heterogeneous with more intense staining in the center of the tumor nests. In metastatic tumors, the percentage of COX-2-positive tumor cells and the intensity of their staining was low compared with Bowen's disease and SCC. CONCLUSIONS: These results indicate that the intensity of COX-2 staining and its heterogeneous distribution are related to the degree of cellular differentiation and the various phenotypes of tumor cells, but the extent of COX-2 staining did not correlate with the degree of malignancy.     

Immunohistochemical study of cyclooxygenase-2 and p53 expression in skin tumors

Overexpression of cyclooxygenase-2 (COX-2) has been demonstrated in various cancers, including experimentally promoted tumors, gastrointestinal cancers, breast tumors and skin tumors. The mechanism that controls COX-2 expression is not yet clear. Currently, it is reported that COX-2 expression is frequently associated with mutated p53 genes. The goal of this study was to evaluate the expression patterns of COX-2 and p53 in several skin tumors and their correlation. An immunohistochemical method was used to investigate the expression of COX-2 and p53 proteins on formalin-fixed, paraffin-embedded tissue specimens of squamous cell carcinomas (SCC), basal cell carcinomas (BCC), Bowen's disease (BD), actinic keratosis (AK) and porokeratosis. The expression of COX-2 increased in 50% (5/10) of SCC, 80% (8/10) of BCC, 40% (4/10) of BD, 50% (5/10) of AK, and 20% (2/10) of porokeratosis cases. The expression of p53 increased in 90% (9/10) of SCC, 70% (7/10) of BCC, 70% (7/10) of BD, 50% (5/10) of AK, and 40% (4/10) of porokeratosis cases. COX-2 positivity rates of the p53-positive skin tumors were 56%, 100%, 57%, 80% and 25% in SCC, BCC, BD, AK and porokeratosis, respectively. However, the correlation between p53 and COX-2 expression in skin tumors was not statistically significant ( P  > 0.05). Our results indicate that skin COX-2 and p53 may play roles in skin tumors, but that there is no apparent correlation between the two markers.     

p73蛋白在正常人皮肤和表皮肿瘤中的表达

目的 探讨p73蛋白在正常人皮肤和不同表皮肿瘤皮损中的表达及意义。方法 应用免疫组化方法检测19例脂溢性角化病、16例基底细胞癌、11例Bowen病、5例鳞状细胞癌及10例正常人皮肤p73、p53、Ki67的表达。结果 在正常人表皮基底层、毛囊外毛根鞘最外层基底样细胞和皮脂腺生发细胞有p73的表达;在基底细胞癌和脂溢性角化病的基底样细胞、Bowen病中异形性明显的瘤细胞p73呈高表达,鳞状细胞癌和脂溢性角化病中的鳞状细胞呈弱阳性或不表达。脂溢性角化病、Bowen病、基底细胞癌、鳞状细胞癌之间p73蛋白表达差异有统计学意义(H=12.71,P<0.01),其中基底细胞癌的表达最强。Ki67在皮肤肿瘤之间差异也有统计学意义(H=14.12,P<0.01),但p53差异无统计学意义(H=2.058,P>0.05)。在各组样本中,p73的表达与p53、Ki67无显著相关性(P>0.05)。结论 p73蛋白可能在皮肤分化中起重要作用。     

Stromal CD10 expression, as well as increased dermal macrophages and decreased Langerhans cells, are associated with malignant transformation of keratinocytes

Background:  It has become evident that resident stromal cells, such as fibroblasts and inflammatory cells, are involved in the metastatic process, including proliferation or migration of malignant neoplasms. We analyzed CD10+ stromal cells, dermal macrophages and Langerhans cells (LCs) in skin tumors.
Methods:  Immunohistological staining was performed with markers for macrophages (CD68), LC (CD1a), stromal fibroblasts (CD10) and cell proliferation (Ki67) in 12 normal skins (NSs) and 15 cases each of seborrheic keratosis (SK), actinic keratosis (AK), keratoacanthoma (KA), Bowen's disease (BD) and squamous cell carcinoma (SCC).
Results:  All SCCs showed weak to strong stromal CD10 expression, while all NS, SK and AK were negative. Weak CD10 expression was observed in only 2 of 15 samples in both BD and KA. The number of CD68+ cells and Ki67 labeling index in SCC and BD were significantly higher than that in KA, AK and SK. In contrast, the number of LC was lower in SCC and BD. The stromal CD10 expression was significantly correlated with the Ki67 labeling indices and CD68+ cells and negatively correlated with decreased LC.
Conclusions:  The stromal CD10 expression is associated with malignant transformation of keratinocytes together with infiltration of dermal macrophages and loss of LC.     

Expression of bcl-2, p53 and Ki-67 in arsenical skin cancers

To investigate the regulation of apoptosis and proliferation in arsenic-induced skin cancers, we examined the expression of bcl-2. p53, and Ki-67 using immunohistochemical staining. Thirty patients with Bowen's disease (BD), ten with basal cell carcinoma (BCC), eight with squamous cell carcinoma (SCC) and eleven of perilesional normal skin (PLN) of the non-sun exposure sites from endemic area were examined. The results showed that: 1) bcl-2 was expressed in all of the BCC homogeneously, in none of the SCC, and in 12/30 of the BD focally or homogeneously; 2) p53 was expressed in all of the arsenical skin cancers with a labelling index of 75±14% of BD, 50±17% of BCC. 61±15% of SCC, and also in all of the perilesional normal skin with a labelling index of 55±24%; 3) Ki-67 was expressed in all of the skin cancers with labelling index of 58±17% of BD. 12±7% of BCC, 47±21% of SCC, and in 9/11 of PLN with a labelling index of 41±24%. Expression of bcl-2 in BCC or BD is related to the phenotype of germinative basal cell. The constant expression of bcl-2 i early dysplastic cells of BD and the earliest expression of P53 in the basal cells of perilesional normal skin indicate that the initial step of arsenic-induced carcinogenesis is from the basal germinative cells. There is no mutual relationship between bcl-2, p53 or Ki-67 expression in any type of the arsenical skin cancers, but there is a positive correlation between p53 and Ki-67 expression identified in perilesional normal skin. BD had the highest labelling index of p53 and Ki-67.     

组织蛋白酶D在皮肤鳞状细胞癌、基底细胞癌和脂溢性角化病的组织表达及其意义

目的：观察组织蛋白酶D（cathepsinD，CD）在皮肤鳞状细胞癌（SCC）、基底细胞癌（BCC）、脂溢性角化病（SK）的组织表达，分析其表达差异及其意义。方法：用免疫组化SP染色法检测CD在15例SCC、15例BCC、14例SK及10例正常对照皮肤组织中的表达。结果：CD在正常皮肤组织表达为阴性．在SK、BCC、SCC瘤组织中表达依次升高，在SCC、SK之间表达有显著性差异（P〈0．05）；CD在SK、BCC、SCC间质细胞表达阳性率分别为85．7％、66，7％、33．3％。结论：CD的表达水平可能与SCC侵袭和转移有关。     

Cyclooxygenase-2 expression and angiogenesis in squamous cell carcinoma of the skin and its precursors: a paired immunohistochemical study of 35 cases

BACKGROUND: Cyclooxygenase (COX)-2 expression and tumour-induced angiogenesis appear to be increased in squamous cell carcinoma (SCC) of the skin. In other cancers, COX-2 is a pro-angiogenic factor. The association between angiogenesis and COX-2 has not been studied in skin cancer. OBJECTIVES: To assess the onset of increased COX-2 expression and angiogenesis in the multistage carcinogenesis of SCC as well as the correlation between those two parameters. PATIENTS/METHODS: We performed a retrospective paired immunohistochemical analysis of normal skin, actinic keratosis (AK), Bowen's disease (BD) and SCC among 35 individuals. Specimens were considered COX-2 immunopositive when 5% or more of the tumour cells showed clear evidence of immunostaining. To quantify active angiogenesis, we used a Ki-67-CD34 double-labelling immunohistochemical stain and calculated the fraction of proliferating endothelial cells. The Chalkley method was used to determine the microvessel density. To detect hypoxia, a carboanhydrase IX immunostain was used. RESULTS: Compared with normal epidermis (0%), AK (31%), BD (22%) and SCC (40%) were significantly more likely to be COX-2 immunopositive (P < 0.01). The fraction of proliferating endothelial cells and the Chalkley scores paralleled multistage carcinogenesis (P < 0.05 between different stages). COX-2 immunopositivity was fairly correlated with hypoxia and higher proliferating endothelial cell fractions but not with Chalkley counts. CONCLUSIONS: Induction of COX-2 expression and angiogenesis are both early events in the development of SCC. In addition to ultraviolet light, hypoxia and COX-2 may be involved in skin tumour angiogenesis.     

P16 is overexpressed in cutaneous carcinomas located on sun-exposed areas

BACKGROUND: Recently, an increased expression of P16, a cell cycle regulatory tumor suppressor protein, has been demonstrated in cervical squamous neoplasms as a marker of malignancy. In contrast, studies performed in skin carcinomas led to contradictory results. OBJECTIVES: Our first aim was to evaluate P16 expression in different types of non-melanoma skin cancers compared with normal skin and benign tumors. The second aim was to evaluate the relationship between P16 expression and the location of skin tumors (i.e. exposed versus non exposed sites). Finally, we also studied Ki67 expression in skin carcinomas and control biopsies. METHODS: Skin biopsy specimens with typical histologic features of squamous cell carcinoma (SCC; n = 30), Bowen's disease (BD; n = 17), basal cell carcinoma (BCC; n = 10), seborrheic keratosis (SK; n = 10) and normal human skin (NHS; n = 9) were obtained from 76 patients seen at our institution between 2001 and 2003. In all cases, P16 and Ki67 expression were evaluated by immunohistochemistry and image analysis. RESULTS: P16 overexpression was observed in 58% of cutaneous carcinomas (SCC: 60%; BD: 58%; BCC: 50%) versus 0% of SK or NHS (0%) (p = 0.006). Ki67 expression in over 5% of tumour cells was observed in 69% of cutaneous carcinomas (SCC: 54%; BD: 76%; BCC: 80%) versus 16% in the group including SK (30%) and NHS (0%) (p = 0.04). Overexpression of P16 was associated with a high rate of Ki67 positive tumour cells in 23/57 malignant skin tumors (40%). Both P16 was associated and Ki-67 were negative in 7/57 cases (12%). Sixty-eight percent of tumors located on sun-exposed areas versus 23% of those located on non sun-exposed areas overexpressed P16 (p = 0.02). CONCLUSION: Our study demonstrated that the expression of P16 and Ki67 is associated with skin carcinomas. No difference was observed according to histological types of carcinomas, suggesting that P16 and Ki67 expression did not correlate with the degree of proliferation and malignancy. Within cutaneous carcinoma specimens, P16 overexpression was significantly associated with the location on sun-exposed areas, suggesting a possible induction of P16 overexpression by UV radiation.     

桥粒芯糖蛋白1在不同表皮肿瘤中表达的研究

目的：了解桥粒芯糖蛋白1与表皮肿瘤的病理及生物学行为之间的关系。方法：采用过氧化物酶标记的链霉卵白素免疫组织化学染色方法，检测了桥粒芯糖蛋白1(Dsgl)在鳞状细胞癌、基底细胞癌、Bowen病、日光角化病、角化棘皮瘤、脂溢性角化病及正常皮肤中的表达。结果：Dsgl在正常表皮中显著表达；在鳞状细胞癌和基底细胞癌的肿瘤组织中表达显著减弱或消失；在Bowen病和日光角化病细胞间变区域无表达；在绝大多数角化棘皮瘤、脂溢性角化病中的表达为强而连续的胞膜染色，与正常表皮中的表达相似。结论：皮肤恶性肿瘤中Dsgl的表达显著减弱或消失，可能与肿瘤的侵袭性和转移有关，Dsgl可能对表皮良、恶性肿瘤的鉴别诊断具有一定价值。