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随着甲基叔丁基醚(MTBE)的广泛使用,其致突变效应及遗传毒性已引起关注。本实验采用单细胞凝胶电泳(SingleCelgetAsay;SCG)技术测试国产MTBE对人的外周血有核细胞的DNA损伤作用。方法(1)样品MTBE1:国产,纯度97%;MTB... 相似文献
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经GPMT法检测,25%异佛尔酮二异氰酸酯(IPDI)对豚鼠的致敏率为50%,属中等致敏物。IPDI对小鼠脾脏抗体生成细胞有抑制作用,但可刺激豚鼠T淋巴细胞及淋巴细胞转化功能。Ames试验阴性,166mg/kgIPDI可诱导大鼠骨髓染色体畸变、小鼠骨髓嗜多染红细胞微核率增高、小鼠骨髓细胞SCE增加及UDS试验阳性。IPDI53~424mg/L对小鼠骨髓细胞DNA合成有明显抑制作用,表明IPDI对体细胞具有断裂剂活性和损伤DNA作用。小鼠精子畸形试验和睾丸染色体畸变试验表明对生殖细胞无明显损伤作用。大鼠致畸试验说明IPDI为不致畸物。职业接触IPDI人群调查表明,接触者有明显的粘膜和呼吸道刺激症状,高浓度接触者肺功能(FVC、FEV1.0)较对照组有下降趋势,其他心、肝、肾功能、外周血淋巴细胞亚群检查、染色体畸变分析、内科、神经系统检查均与对照组无显著性差异。 相似文献
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甲基叔丁醚及其代谢产物对HL—60细胞的遗传毒性测定 总被引:19,自引:0,他引:19
应用单细胞凝胶电泳法(SCGE)测定新型汽油添加剂甲基叔丁醚(MTBE)及其代谢产物叔丁醇(TBA)、α-羟基异丁酸(HIBA)和甲醛引起的人白血病细胞(HL-60细胞)DNA损伤,以乳酸脱氢酶的释放评价其细胞毒性。结果显示,MTBE、TBA和HIBA在1~30mmol/L浓度范围内能够引起DNA损伤并有明显的剂量反应关系;另一代谢产物甲醛在浓度5μmol/L时即可产生DNA损伤,但浓度升高时出现DNA迁移减少。提示MTBE及其代谢产物可能具有遗传毒性。然而在引起DNA损伤的剂量范围内却没有观察到MTBE、TBA和HIBA的细胞毒性,而甲醛则表现明显的细胞毒性作用 相似文献
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应用单细胞凝胶电泳技术检测辐射致外周血淋巴细胞DNA损伤 总被引:4,自引:0,他引:4
应用单细胞凝胶电泳试验 (SCGE) 分别检测了受γ射线照射后小鼠及从事X 射线探伤工人的外周血淋巴细胞DNA 单链断裂(ssb) , 结果发现: 0-5Gy 和5-0Gy 的γ射线照射组小鼠, 外周血淋巴细胞彗星百分率显著高于对照组; 从事X 射线探伤工人的外周血淋巴细胞彗星百分率也显著升高。提示SCGE 检测的DNAssb 可作为射线对健康影响的早期生物学指标。 相似文献
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猕猴桃汁抗环磷酰胺致突变作用的机理 总被引:12,自引:1,他引:11
目的用大鼠外周血双核淋巴细胞微核测试法(CBMNT),在哺乳动物整体水平,研究猕猴桃汁抗环磷酰胺(CP)的致突变作用以及生物转化Ⅱ相酶的作用。方法测定大鼠外周血双核淋巴细胞微核细胞率及肝组织中总谷胱甘肽硫转移酶(GST)、尿苷二磷酸葡萄糖醛酸转移酶(UDPGT)、谷氨酰胺转肽酶(γGT)活性。结果猕猴桃汁对CP诱发的大鼠外周血双核淋巴细胞微核细胞率有显著抑制作用,能明显诱导大鼠肝脏总GST、UDGTP活性,但对γGT活性无显著影响。大鼠外周血双核淋巴细胞微核细胞率与总GST、UDGTP活性呈明显负相关。结论猕猴桃汁抗CP致微核形成作用的机理可能是通过诱导机体外来化合物代谢解毒酶系,加速CP的代谢灭活 相似文献
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微囊藻毒素LR对小鼠肝脏和淋巴细胞的损伤效应 总被引:6,自引:0,他引:6
[目的]研究不同剂量微囊藻毒素LR(MCLR)经灌胃途径对小鼠肝细胞及外周血淋巴细胞细胞的损伤效应。[方法]生化法测定血清中谷丙转氨酶(ALT),谷草转氨酶(AST),碱性磷酸酶(AKP),γ-谷氨酸转移酶(γ-GT)和乳酸脱氢酶(LDH)含量,单细胞凝胶电泳试验(彗星试验)测定外周血淋巴细胞DNA迁移长度。[结果]MCLR在1μg/L浓度下即可引起ALT,LDH的显著升高;在达到100μg/L浓度时可引起AKP明显升高;但各剂量组AST,γ-GT与对照组相比无显著差异;不同剂量MCLR均可引起外周血淋巴细胞DNA迁移长度增加,且有剂量-反应关系。[结论]MCLR在1μg/L低浓度下对小鼠肝细胞及外周血淋巴细胞可产生一定损伤作用。 相似文献
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酸角对Brdu和MNNG致突变作用的影响 总被引:2,自引:0,他引:2
用人外周血淋巴细胞进行姐妹染色单体互换(SCE)和微核(MN)试验,研究酸角抗Brdu和MNNG的诱变作用。结果酸角能降低Brdu诱发的SCE,试验组的SCE频率随酸角剂量增加而降低,与对照组比有统计学意义(P〈0.05);酸角能明显降低MNNG诱发的SCE和MN,试验组的SCE频率和MN发生率与阳性对照组比较差异非常显著(P〈0.01),并有明确的剂量-反应关系,SCE抑制率为62.38 ̄94. 相似文献
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吸烟与温石棉单独及联合作用对人胚肺细胞DNA链断裂的影响 总被引:10,自引:1,他引:9
目的研究吸烟与温石棉对细胞的遗传毒性。方法采用单细胞凝胶电泳技术(SCGE),在体外实验中测定了香烟烟雾溶液(CSS)与温石棉(CH)单独及联合作用于人胚肺(HEL)细胞时DNA链断裂的情况。结果CSS与CH单独与HEL细胞作用1小时,均可使DNA链断裂明显增多,当二者以3种不同的剂量组合联合作用时,均可协同增加DNA链断裂。结论CSS和CH单独作用均为肿瘤启动剂;当二者联合作用时可协同启动肿瘤的发生。 相似文献
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汞化合物对人体外淋巴细胞的DNA损伤的比较研究 总被引:1,自引:0,他引:1
惠秀娟 《中国公共卫生学报》1998,17(4):240-241
对人体外淋巴细胞用不同浓度的甲基氯化汞(MMC)和氯化汞(MC)处理1h,观察DNA损伤的程度,采用细胞琼脂糖凝胶电泳技术(SCGE)进行比较研究。这两种汞化物均不同程度的显示了剂量反应关系。DNA损伤的程度从10-5mol/L起与空白对照相比,呈现有意义的增加,细胞DNA断裂频率的增加与毒性呈现为线性增长的关系。剂量愈高,则DNA的彗尾链愈长,两种化合物在同样的实验条件下显示了相似的分裂剂的潜能,氯化汞表现出的遗传毒性更明显 相似文献
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Trends in meningococcal disease in Italy in 1988 总被引:1,自引:0,他引:1
Meningococcal disease in Italy decreased 15% in 1988 from the previous year (290 vs. 342 cases). The decline was particularly evident in military cases (1.7/100,000 in 1988 vs. 5/100,000 in 1987) reflecting the full coverage of bivalent serogroup (A + C) meningococcal polysaccaride vaccine in army recruits, achieved since January 1988. The highest proportion of cases was seen in people older than 25 years of age (25%). Serogroup C constituted 60% of the isolates, while 19% belonged to serogroup B. The proportion of strains resistant to sulphonamides was 45%, while 15% were resistant to Minocycline and none to Rifampin. Out of the five military cases, only one (due to serogroup C) was attributable to the vaccine failure. A single coprimary case, but no secondary cases occurred among civilians. These findings are consistent with the trends reported in Italy in the previous years. 相似文献
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C L Rümke 《Nederlands tijdschrift voor geneeskunde》1985,129(51):2469-2471
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Leon Braier Ashley Levy Klaus Dror Asher Pardo 《American journal of industrial medicine》1981,2(2):119-123
Determinations of benzene concentration in blood and of phenol in urine were made by head-space gas chromatography techniques on samples taken near the end of the work day from two groups of workers potentially exposed to low levels of benzene in the work-place atmosphere. Preliminary results suggest that benzene in blood is more reliable than phenol tests for assessing both exposure and uptake of benzene. Normal values of phenol in urine (10 mg/liter or less) were found in nearly all those cases in which benzene was detected in the blood. 相似文献
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Asthma is an inflammatory disorder of the airway. The airway inflammation of asthma is typically an allergic inflammation characterized by cells and mediators described as a "Th2" inflammatory response. There is a growing body of evidence describing changes in the function of immune cells upon aging, a phenomenon referred to as "immunosenescence". Several studies utilizing animal models and human subjects with asthma have begun to explore age-related effects on the airway inflammation in asthma. This review explores the existing data on the presence and effects of immunosenescence or age-related changes in immune function in asthma. 相似文献
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《Journal of food composition and analysis》2000,13(4):505-510
Results from a study conducted in Italy concerning the updating of composition data of beef consumed in Italy are reported here. The study required a very wide sampling plan with regard to both animals and cuts to analyse. Data related to lipid composition of the most widely used type of beef consumed in Italy (young bull, 16–22 months old) show a reduction in total lipid content (which, according to the cuts, ranges between 1.8 and 10.2%) and a substantial change in fatty acid composition, with a high % of polyunsaturated fatty acids (mean 20% of total fatty acids). Such data have been confirmed by other studies conducted in Italy, and are in contrast with data reported in the tables of food composition from other countries, reporting a fatty acid composition characterized by a high fatty acid saturation degree. The occurrence of long-chain polyunsaturated fatty acid is probably due to the high ratio of phospholipids: triglycerides, occurring when the content in intramuscular lipid is very low; to the young age of animals; to the reduced activity of the rumen, where saturation of fatty acids occurs. It may be concluded that a fatty acid composition, which is particularly rich in polyunsaturated fatty acids, seems typical for Italian products. 相似文献
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Nurses are known to be exposed to occupational stress. However, occupational stress is not well documented for nurses in psychiatric institutions in Taiwan. A cross-sectional study was conducted to explore the work-related stress and risk factors of nurses in psychiatric institutions in Taiwan. A structured questionnaire was distributed to nurses at five state-owned psychiatric hospitals in Taiwan in 2001. Demographic information, working environment, and personal health status were inquired. Occupational stress was assessed based on the Chinese version of Job Content Questionnaire (JCQ). General health status and mental health were evaluated by the International Quality of Life Assessment Short Form-36 (IQOLA SF-36). A total of 573 questionnaires were disseminated to nurses and 518 (90.4%) were satisfactorily completed by nurses, including 408 female full-time nurses who had been in their current work for more than 6 months. In the past one month, 17.2% of nurses reported being under significant stress often or always. Assault episodes were reported by 45.1% of nurses in the past 6 months. Among the nurses, 16.9%, 25.2%, 50.0%, and 7.8% belong to the "High strain", "Low strain", "Active", and "Passive" groups, respectively. Perceived occupational stress was associated with young age, widowed/divorced/separated marital status, high psychological demand, low workplace support, and threat of assault at work. Lower general health score was associated with low job control, high psychological demand, and perceived occupational stress. A lower mental health score was associated with low job control, high psychological demand, low workplace support, and perceived occupational stress. We concluded that nurses in psychiatric institutions are under significant stress related to work factors. 相似文献
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This historical and bibliographic study aimed to understand how Nursing was organized to support care in transplantation. The HISA, LILACS, BDENF, PERIENF and DEDALUS databases were consulted, and thirteen references were found, ten of which were scientific articles, two were master's dissertations and one was a doctoral thesis. The span of time chosen for study ranges from the date of the first kidney transplant in Brazil (1965), to the date of publication of the last scientific article found in the databases mentioned above (2003). After reading these articles, the ones that were similar in topic were grouped together, thus creating the thematic axis for the presentation of the results. The results showed that the Nursing profession has played an important and active role in transplants ever since the first procedure in 1965. 相似文献