细胞因子体外扩增脐血CD^＋34细胞移植SCID鼠人类免疫功能的 …

ObjectiveTo explore the hematopoietic function of cytokine-expanding CD⁺₃₄ cells from umbilical cord blood in vitro.MethodCord blood cells expanded with FLT3(FMS-like tyrosine kinase 3)-ligand and thrombopoietin were transplanted in SCID(severe combined immune-deficient)mice. Human mature cell surface markers were detected with flow cytometry and used as indicator of succeeding transplantation.ResultAfter 2 and 3 weeks culture,the number of CD⁺₃₄ cells increased approximately 10 and 37 folds. After transplantation with 6.6×10⁴ to 6.9×10⁵ CD⁺₃₄ cells,respectively the percentage of human CD⁺₄₅ cells in the bone marrow of all 10 SCID mice is 0.2%～4.0%. The percentage of human CD⁺₃ cells in the peripheral blood and spleen of 6 SCID mice is 0.1%～2.3%. The percentage of human CD⁺₁₉ cells in the peripheral blood and spleen of 3 SCID mice are 0.3%～1.3%.ConclusionCD⁺₃₄ cells expanded with FLT3-ligand and thrombopoietin are potential engraft used for reconstitution of both hematopoietic and immunologic function of SCID mice.     

Catheter ablation of atrial fibrillation in elderly population

Background Although elderly patients have been included in published series of catheter ablation for atrial fibrillation (AF), clinical benefit and safety remain still less defined in this population. A retrospective analysis of the results of catheter ablation for AF in a large volume center focused on comparison of elderly patients with the rest of the patient cohort was conducted in this study. Methods Consecutive patients who underwent catheter ablation for AF between January 2001 and December 2016 were analysed. A total population of 3197 patients was dichotomized by the age of 70 years (394 elderly vs. 2803 younger subjects). Patients were followed in terms of arrhythmia status and sur?vival for a median period of 18 vs. 21 and 35 vs. 57 months, respectively. Results Elderly patients were more frequently females (49% vs. 29%, P < 0.0001), had a history of hypertension (79% vs. 57%, P < 0.0001), diabetes (16% vs. 11%, P < 0.01), stroke (9% vs. 6%, P < 0.01), coronary/peripheral artery disease (14% vs. 8%, P < 0.0001), and CHA2DS2-VASc score (3.1 ± 1.3 vs. 1.5 ± 1.2 s, P < 0.0001). Major complications were more frequent in elderly (5.3% vs. 3.2%, P = 0.03); however, this difference was driven by vascular complications (3.6% vs. 1.9%, P = 0.04). There were comparable rates of cerebrovascular (0.3 vs. 0.3%) or nonvascular complications (1.8 vs. 1.2%). Good arrhythmia control was inferior in elderly patients as compared with the rest of the cohort, both without and with antiarrhythmic drugs: 44.2% vs. 58.2% (P < 0.0001) and 78.2 vs. 83.2% (P < 0.01), respectively. Poor arrhythmia control was associated with relative risk of all-cause mortality of 2.7 (95% CI: 1.1–6.4) in elderly patients and 1.4 (95% CI: 0.9–2.0) in younger subjects. Conclusions Catheter abla?tion for AF in elderly patients is safe although somewhat less effective. Good arrhythmia control is associated with better survival, especially in elderly patients.     

内地高辛、钠、钾、镁与高血压病胰岛素抵抗的相关性

目的 :探讨内地高辛及钠、钾、镁在高血压病胰岛素抵抗病理机制中的作用。方法 :测定 30例高血压病患者(EH组 )的空腹血浆葡萄糖 (FPG)、胰岛素 (FINS)水平 ,同时测定血清内地高辛 (EDX)及细胞内 Na+、 K+、 Mg2 +含量并与 2 6例正常血压对照者 (NC组 )作对照。结果 :EH组 FPG、FINS、EDX、Na+水平显著高于 NC组 (P<0 .0 5 ,<0 .0 1,<0 .0 1、 <0 .0 0 1) ,胰岛素敏感性指数 (ISI)、 K+、 Mg2 +显著低于 NC组 (P<0 .0 0 2 ,<0 .0 1,<0 .0 0 1) ;EH组 FINS与体重、体重指数 (BMI)、臀围、 EDX呈显著正相关 (r=0 .5 2 ,0 .35 ,0 .45 ,0 .41;P<0 .0 0 5 ,<0 .0 5 ,<0 .0 1,<0 .0 5 ) ,ISI与体重、BMI、臀围呈显著负相关 (r=- 0 .5 8,- 0 .42 ,- 0 .5 7;P<0 .0 0 1,<0 .0 5 ,<0 .0 1)。结论 :高血压患者血胰岛素水平与内地高辛、肥胖呈显著正相关 (P<0 .0 5～ <0 .0 0 5 ) ;ISI与肥胖呈显著负相关。     

我国伊蚊族蚊类记录的校订及新分类系统的建议（双翅目∶蚊科）

本文根据国外蚊虫分类经验,对我国伊蚊族蚊类以往记录171种的分类地位作了校订,其中160种可归入我国伊蚊族新分类系统的29个属中,另外11种地位未定,暂按旧分类地位列名表供查考。我国伊蚊族新分类系统包括伊蚊属Aedes、阿蚊属Armigeres、艾蚊属Ayurakitia、博蚊属Bothaella*、布蚊属Bruceharrisonius*、环喙蚊属Christophersiomyia*、科蚊属Collessius*、丹蚊属Danielsia*、唐蚊属Downsiomyia*、箭阳蚊属Edwardsaedes*、纷蚊属Finlaya*、弗蚊属Fredwardsius*、贾蚊属Gilesius*、领蚊属Heizmannia、喜蚊属Himalaius*、霍金蚊属Hopkinsius*、呼蚊属Hulecoeteomyia*、连蚊属Jihlienius*、奈蚊属Kenknightia*、陆蚊属Luius*、霉蚊属Mucidus*、新黑蚊属Neomelaniconion*、骚扰蚊属Ochlerotatus、花蚊属Phagomyia*、盾蚊属Scutomyia*、覆蚊属Stegomyia*、田中蚊属Tanakaius*、尤蚊属Udaya和奇阳蚊属Verrallina等29属,其中22属（*）为我国新记录。此外,还对《中国动物志,昆虫纲第8卷,双翅目：蚊科上卷》记载的4种伊蚊作了重要订正。安图伊蚊Ae. （Edw.） antuensis应是平坝箭阳蚊Ed. pingpaensis的同物异名。滇西伊蚊Ae. （Sin.） occidentayunnanus、黄背伊蚊Ae. （Och.） flavidorsalis和亚同伊蚊Ae. （Fin.） subsimilis分别订正为滇西领蚊Hz. （Mat.） occidentayunnana、白色骚扰蚊Oc. albineus和亚同尤蚊Ud. subsimilis。     

State of knowledge of helminth fauna of freshwater fishes of Poland

A total 89 fish and lamprey species has been recorded from Polish freshwater habitats. Twenty-seven of them (30.3%) have not been surveyed for parasitic helminthes. Some of the latter fishes are either rare or not easily accessible. Other live only in specific habitats in scattered localities. An important obstacle for studying parasite faunas of some fishes may be their status on an endangered species. Among the non-surveyed fishes, are those which have been relatively recently introduced to Poland or migrated there on their own. The present paper attempts to review all hitherto not studied helminthologically fish species, their habitats, localities and current protection status.     

Lack of correlation of degree of villous atrophy with severity of clinical presentation of coeliac disease

BACKGROUND AND AIM: Both the clinical presentation and the degree of mucosal damage in coeliac disease vary greatly. In view of conflicting information as to whether the mode of presentation correlates with the degree of villous atrophy, we reviewed a large cohort of patients with coeliac disease. PATIENTS AND METHODS: We correlated mode of presentation (classical, diarrhoea predominant or atypical/silent) with histology of duodenal biopsies and examined their trends over time. RESULTS: The cohort consisted of 499 adults, mean age 44.1 years, 68% females. The majority had silent coeliac disease (56%) and total villous atrophy (65%). There was no correlation of mode of presentation with the degree of villous atrophy (p=0.25). Sixty-eight percent of females and 58% of males had a severe villous atrophy (p=0.052). There was a significant trend over time for a greater proportion of patients presenting as atypical/silent coeliac disease and having partial villous atrophy, though the majority still had total villous atrophy. CONCLUSIONS: Among our patients the degree of villous atrophy in duodenal biopsies did not correlate with the mode of presentation, indicating that factors other than the degree of villous atrophy must account for diarrhoea in coeliac disease.     

高血压降压治疗目标的再认识

根据传统的高血压水平的定义,1993年WHO高血压治疗指南提出血压控制目标为<140/90mm Hg(1mm Hg=0.133kPa),但是并非所有患者都必须将血压降至同一水平,而应根据患者情况进行个体化治疗。Framingham进行的一项长达10～12年的心血管事件研究发现,第5年后,正常上限血压[收缩压(SBP