Detection of human papillomavirus-16 in ovarian malignancy

Human papillomavirus is the causal factor for cervical cancer. However, the role of HPV infection in ovarian cancer is unclear. This study aimed to determine the presence of human papillomavirus-16 (HPV-16) in ovarian cancer tissues. Archived human ovarian cancer tissues (N=54 cases, 50 are epithelial cancer, four are nonepithelial cancer) embedded in paraffin blocks were used. Controls are 30 nonmalignant ovarian tissue blocks. In situ hybridisation (ISH) and immunohistochemistry (IHC) were used to detect the presence of HPV-16 and p53 expression. In all, 52 or 36% of the epithelial ovarian tumours detected by ISH or IHC, respectively, were HPV-16 E6 positive. In contrast, only 6.7% of normal ovarian tissues were HPV-16 positive proved by ISH. Human papillomavirus-16 infection was significantly higher in cancer tissues compared to controls with an odds ratio of 16.7 (95% confidence interval [CI]=3.2-71.4, P<0.01). No significant correlation between HPV-16 infection and histological types of cancer was found (P>0.05). p53 gene expression was detected in 42% epithelial ovarian cancers. No correlation between p53 expression and HPV-16 infection was found. The results showed the presence of HPV-16 E6 in ovarian carcinoma, suggesting that HPV infection might play a role in ovarian carcinogenesis.     

微小病毒B19感染与甲状腺乳头状癌的关系

目的：探讨人类微小病毒B19感染（parvovirus B19，B19）与甲状腺乳头状癌（Papillary thyroid carcinoma,PTC）的关系。方法：对38例手术切除的PTC患者甲状腺组织（其中30例伴有周围正常甲状腺组织）及16例甲状腺腺瘤患者腺瘤旁正常甲状腺组织，分别用巢式聚合酶链反应（nested polymerase chain reaction，nPCR）、原位杂交（in situ hybridization，ISH）和免疫组织化学（immunohistochemistu，IHC）检测B19病毒DNA和病毒蛋白的表达。结果：nPCR扩增出173bp的B19特异性目的条带。ISH和IHC检测出B19病毒DNA和蛋白分别位于肿瘤细胞胞核和胞浆内在PTC中，nPCR、ISH和IHC阳性率分别为97.4％（37／38）、78.9％（30／38）和63.2％（24／38）．而腺瘤旁正常甲状腺组织中，阳性率分别为43．8％（7／16）、12．5％（2／16）和6．25％（1／16）：两者比较差异有统计学意义（3种方法均P〈0．001）：癌旁甲状腺组织ISH和IHC阳性率分别为23.3％（7／30）和10．0％（3／30），与之相对应的30例PTC（ISH：80．0％；IHC：60．0％）相比，差异有统计学意义（两者均P〈0.001）。结论：PTC患者甲状腺肿瘤组织中B19感染率明显高于腺瘤旁和癌旁正常甲状腺组织，提示B19感染在PTC的发生中可能起重要的作用。     

人乳头状瘤病毒16 型DNA 在乳腺癌组织中表达的研究

 目的　探讨人乳头状瘤病毒 (HPV) 1 6型感染与人乳腺癌病因学的关系。方法　采用原位分子杂交技术检测本地区人口女性乳腺癌和正常乳腺组织中的 HPV1 6型 DNA。结果　乳腺癌和正常乳腺组织中 HPV1 6型 DNA的阳性率分别为 52 .0 %和 2 0 .0 % ,多种组织学类型的乳腺癌组织中有 HPV1 6型 DNA的存在且以整合型感染为主。结论　本地区女性乳腺组织中有HPV1 6型 DNA感染的存在 ,HPV1 6型感染与本地区乳腺癌的发生、发展密切相关。     

二肽基肽酶Ⅳ与卵巢上皮性癌临床病理特征和预后的关系

目的 探讨卵巢上皮性癌组织中二肽基肽酶N(DPPIV)的表达及临床意义.方法 采用免疫组化法榆测378例卵巢上皮性癌组织中DPPIC蛋白的表达,采用原位杂交法检测86例卵巢上皮性癌组织中DPPIV mRNA的表达,42例正常卵巢组织作为对照.结果 DPPIV蛋白在卵巢上皮件癌组织中的总阳性表达率为92.9%(351/378),在正常卵巢组织中的总阳性表达率为59.5%(25/42),差异有统计学意义(P=0.001).DPPIV蛋门的表达与患者的发病年龄、肿瘤分化程度和FIGO分期无关(均P＞0.05),而与肿瘤的组织学类型有关(P=0.005).DPPIC蛋白的表达水平越高,患者的生存期越短(P=0.023).DPPIV mRNA在卵巢上皮性癌组织巾的总阳性表达率为97.7%(84/86),在正常卵巢组织中的总阳件表达率为64.3%(27/42),差异有统计学意义(P＜0.05).卵巢上皮性癌组织中DPPIV蛋白和mRNA的表达与定位一致,且二者旱止相关(P=0.001).结论 DPPIV在卵巢上皮性癌的发生发展中起着一定作用,其表达水平可能成为影响患者预后的因素.     

Prostate stem cell antigen (PSCA) expression in human prostate cancer tissues: implications for prostate carcinogenesis and progression of prostate cancer

OBJECTIVE: Prostate stem cell antigen (PSCA) is a recently defined homolog of the Thy-1/Ly-6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface antigens. The objective of the present study was to examine the expression status of PSCA protein and mRNA in clinical specimens of human prostate cancer (PCa) and to validate it as a potential molecular target for diagnosis and treatment of PCa. METHODS: Immunohistochemical (IHC) and in situ hybridization (ISH) analyses of PSCA expression were simultaneously performed on paraffin-embedded sections of 20 benign prostatic hyperplasia (BPH), 20 prostatic intraepithelial neoplasm (PIN) and 48 prostate cancer (PCa) tissues, including 9 androgen-independent prostate cancers. The level of PSCA expression was semiquantitatively scored by assessing both the percentage and intensity of PSCA-positive staining cells in the specimens. We then compared the PSCA expression between BPH, PIN and PCa tissues and analyzed the correlations of PSCA expression level with pathological grade, clinical stage and progression to androgen-independence in PCa. RESULTS: In BPH and low grade PIN, PSCA protein and mRNA staining were weak or negative and less intense and uniform than that observed in high grade PIN (HGPIN) and PCa. Moderate to strong PSCA protein and mRNA expression were noted in 8 of 11 (72.7%) HGPIN and in 40 of 48 (83.4%) PCa specimens examined by IHC and ISH analyses, and their statistical significance was compared with BPH (20%) and low-grade PIN (22.2%) specimens (P < 0.05). The expression level of PSCA increased with a higher Gleason grade, advanced stage and progression to androgen-independence (P < 0.05). In addition, IHC and ISH staining revealed a high degree of correlation between PSCA protein and mRNA overexpression. CONCLUSIONS: Our data demonstrate that PSCA as a new cell surface marker is overexpressed in a majority of cases of human PCa. PSCA expression correlates positively with adverse tumor characteristics, such as increasing pathological grade (poor cell differentiation), worsening clinical stage and androgen-independence and speculatively with prostate carcinogenesis. PSCA may possess prognostic utility and may be a promising molecular target for diagnosis and treatment of PCa.     

Mycoplasma hyorhinis in gastric cancer

Mycoplasmas are the smallest self-replicating organisms in the world. Although they belong to prokaryote, Mycoplasmas are distinguished phenotypically from bacteria by their minute size (100-800 nm) and total lack of a cell wall. Mycoplasmas are widespread in nature and usually exhibit organ and tissue specificity. As a low-virulence organism, infections with pathogenic Mycoplasmas are rarely of the fulminant type but rather follow a chronic course. The relationship between Mycoplasmas and c…     

Detection of Gene Amplification by Multiplex Ligation- Dependent Probe Amplification in Comparison with In Situ Hybridization and Immunohistochemistry

Gene amplification is an important mechanism in the development and progression of cancer. Currently, gene amplification status is generally determined by in situ hybridization (ISH). Multiplex ligation-dependent probe amplification (MLPA) is a PCR-based method that allows copy number detection of up to 50 nucleic acid sequences in one reaction. The aim of the present study was to compare results for HER2, CCND1, MYC and ESR1 gene amplification detected by MLPA with fluorescent in situ hybridization (FISH) and chromogenic in situ hybridization (CISH) as clinically approved methods. Tissue samples of 170 invasive breast cancers were collected. All were ER positive. Tissue samples had previously been tested for HER2 using immunohistochemistry. Amplification of the selected genes were assessed using MLPA, FISH and CISH and results were compared. HER2 MLPA and ISH results were also compared with HER2 immunohistochemistry (IHC) which detects protein overexpression. Amplification of HER2, CCND1, MYC and ESR1 by MLPA were found in 9%, 19%, 20% and 2% of samples, respectively. Amplification of HER2, CCND1, MYC and ESR1 by FISH was noted in 7%, 16%, 16% and 1% of samples, respectively. A high level of concordance was found between MLPA/ FISH (HER2: 88%, CCND1: 88%, MYC: 86%, ESR1: 92%) and MLPA/ CISH (HER2: 84%). Of all IHC 3+ cases, 91% were amplified by MLPA. In IHC 2+ group, 31% were MLPA amplified. In IHC 1+ group, 2% were MLPA amplified. None of the IHC 0 cases were amplified by MLPA. Our results indicate that there is a good correlation between MLPA, IHC and ISH results. Therefore, MLPA can serve as an alternative to ISH for detection of gene amplification.     

Assessing HER2 amplification in breast cancer: findings from the Australian In Situ Hybridization Program

In August 2006, the Australian government approved subsidized trastuzumab therapy for human epidermal growth factor receptor 2 (HER2)-positive early breast cancer, and it was mandated that HER2 testing should be performed using in situ hybridization (ISH) rather than immunohistochemistry (IHC). Here we review results of the first regulated, nationwide program to provide HER2 ISH testing for all newly diagnosed breast cancer patients, with a particular emphasis on cases where IHC and ISH results were discordant. Data from all laboratories participating in the program were collated. Cases with an equivocal ISH test result [by chromogenic ISH (CISH) or silver ISH (SISH)] were tested centrally by fluorescence ISH. Most laboratories also performed HER2 IHC, and 200 cases with discordant IHC and ISH results were selected for further analysis in a central laboratory. A total of 26 laboratories were involved and 53,402 tests were reported. Over a 4-year period the HER2 positivity rate decreased for primary cancers from 23.8 to 14.6 %, but remained relatively constant for samples from metastases. Average ISH reporting times were <5 days for all yearly reporting periods. Test-repeat rates decreased for CISH (8.9-3.6 %) and SISH (13.7-8.4 %). Only 12 of 196 cases remained discordant after retesting in a central laboratory. These findings demonstrate the successful implementation of a regulated, national program that continues to collect data on HER2 status. The results also highlight the differences in IHC interpretation between local laboratories and a central, more experienced, laboratory. This model could be used to establish future biomarker-testing programs in other countries.     

Role of Human Papilloma Virus in Oral Tongue Squamous Cell Carcinoma

Background: Human papilloma virus (HPV) is an important risk factor for head and neck cancer, specificallyoropharyngeal cancer, but its association with oral tongue squamous cell carcinoma (SCC) is uncertain. Theobjectives were to determine the HPV16 prevalence in oral tongue SCCs, its integration status and to correlatethe expression of oncogenic proteins with targets. Methods: In this case-control study with oral tongue SCC cases(n=60) and normal oral mucosa (n=46), HPV positivity was determined by polymerase chain reaction (PCR)using consensus and HPV 16 type specific primers and p16 immunohistochemistry (IHC). The viral integrationstatus was determined with primers specific to the E2 gene and in situ hybridization (ISH). Immunohistochemicalanalysis of HPV oncogenic proteins (E6, E7) and their target proteins (p53, pRb, cyclinD1, p16, Notch-1, EGFR)proteins was carried out in HPV positive cases. The data was analyzed with SPSS software (v 11.0). Survivalanalysis was carried out by the Kaplan-Meier method. Results: HPV16 was detected in 48% (n=29) of the casesand none of the controls by PCR assay (p<0.001) while p16 IHC, as a surrogate HPV marker, detected 33%(n=18) of the cases; 18% (n=10) were detected by both the methods. Integration was observed in 83% (n=24)by E2-PCR and 67% (n=18) by ISH. The E6-p53 pathway was active in 33% of the cases; E7-pRb in 52% andboth in 11%. HPV positivity was associated with well-differentiated cancers (p=0.041) and low recurrence rate(p=0.014). Conclusion: Our study confirms a positive correlation of HPV infection with oral tongue cancer.     

乳腺癌前哨淋巴结CK19 mRNA和蛋白的表达

目的 探讨CK19微转移分子在乳腺癌前哨淋巴结(SLN)的表达情况及意义.方法 采用免疫组化S-P法及原位杂交技术检测66例SLN中CK19微转移分子的表达.同时与常规病检法比较其检测敏感性.并比较转移组、微转移组、无转移组患者的临床资料.结果 66例SLN在常规病理检查阴性38例淋巴结中6例表达CK19蛋白15.8%(6/38),8例21.1%(8/38)表达CK19mRNA.原位杂交法与常规病检法转移的检出率相比较,差异有统计学意义(P＜0.05).CK19蛋白和mRNA表达之间差异无统计学意义(P＞0.05).同时乳腺癌转移组与微转移组患者在肿物大小与淋巴管浸润上有相似性,而同无转移组差异有统计学意义(P＜0.05).结论 免疫组化法和原位杂交法较常规病理检查更为敏感.通过免疫组化法和原位杂交法的联合使用,可明显提高乳腺癌SLN微转移的检出率,同时也证明原位杂交法是可靠的,比免疫组化更为敏感的技术.SLN微转移有可能作为肿瘤预后的指标.     

揭阳市食管癌p16基因表达与缺失的研究

 目的　了解食管癌高发区揭阳市食管癌患者 p16基因的表达与缺失情况。方法　采用免疫组化、PCR及原位杂交技术检测 6 0例食管癌标本中 p16蛋白表达、基因纯合性缺失及其mRNA信号表达情况。结果　 (1)有 2 6例 (43.3% ) p16蛋白表达阳性 ,且有淋巴结转移与无转移之间蛋白表达差别显著 (P =0 .0 4 3) ;(2 )PCR检测外显子 2缺失有 2 7例 (45 % ) ,外显子 1缺失有 15例 (2 5 % ) ;(3)原位杂交检测 10 / 13例与免疫组化结果一致。结论　p16基因表达缺失与该组食管癌的浸润、转移有相关 ;外显子 2的缺失可能是 p16基因产物丢失的主要机制。     

卵巢癌组织中P16的表达及其临床意义

 目的:探讨抑癌基因P¹⁶与卵巢癌生物学行为的关系及在卵巢癌中表达的临床意义。方法:采用S-P免疫组织化学方法,检测19例卵巢癌,10例正常卵巢组织中抑癌基因P¹⁶的表达,结果:19例卵巢癌,P¹⁶检出率为63.2%,3例晚期卵巢癌表达阴性,4例Ic期以上低分化卵巢癌表达弱阳性,正常对照组,80%阳性表达,10%弱阳性表达。研究提示:P¹⁶的突变与缺失可能与卵巢癌的发生发展密切相关,与临床分期、病理分级密切相关。     

Amplification and specific expression of T-bet gene in nasal NK/T-cell lymphoma

To define the specific genetic alterations in nasal natural killer/T-cell lymphoma (N-NK/T-L), the assay of restriction landmark genomic scanning (RLGS), a genome-wide method, was used to investigate a pair of genomic DNA from N-NK/T-L cells and peripheral blood leukocytes of the same patient. The intensified spots in displayed N-NK/T-L gel of RLGS were subjected to bioinformatic analysis by virtual genome scan (VGS) and one of candidate spots proved to be T-bet gene (T-box expressed in T-cell). The pair of samples was consequently analysed by Southern hybridization, revealing genomic amplification of T-bet but not different status of DNA methylation. The amplification of T-bet was also discovered in other cases of N-NK/T-L by dot blotting. The expression of T-bet was investigated by in situ hybridization (ISH) and immunohistochemistry (IHC). The results showed that T-bet predominantly expressed in N-NK/T-L (ISH: 90.0%, 18/20; IHC: 80%, 16/20), while few cases of B-cell lymphoma (ISH: 11.8%, 2/17; IHC: 17.6%, 3/17) or T-cell lymphoma (ISH: 33.3%, 2/6; IHC: 16.7%, 1/6) were positive. The difference of T-bet expression in either ISH or IHC between N-NK/T-L and B-cell lymphoma or T-cell lymphoma was statistically significant (P < 0.05). Moreover, the expression of T-bet was not detected in normal spleen tissue and chronic inflammatory nasal mucosa. The results suggested that the amplification of T-bet gene or resulting its over-expression of T-bet gene might be involved in the development of N-NK/T-L and thus it should be worth confirming whether the over-expression of T-bet be helpful to the diagnosis of N-NK/T-L in further study.     

卵巢癌中PKC和P-gp 的表达及其临床意义

 目的 探讨卵巢癌组织蛋白激酶C(PKC)和P-糖蛋白(P-gp)表达及其临床意义。方法 用免疫组化S-P法检测35例卵巢癌、20例卵巢良性肿瘤和20例正常卵巢组织中PKC和P-gp的表达，并进行相关临床因素分析。结果 (1)PKC、P-gp在卵巢恶性肿瘤中的表达明显高于在良性及正常组织中的表达；初治和复发病例PKC阳性表达有显著差异(P<0．05)。(2)卵巢癌PKC的表达与临床病理因素无直接关系。(3)卵巢癌中PKC和P-gp的表达有显著相关性(P<0．05)。(4)化疗对PKC表达阳性和阴性卵巢癌患者的有效率分别为23．5％、66．7％(P<0．05)。(5)PKC表达阴性患者的预后优于阳性者(P=0．039)。结论 PKC表达与P—gp的表达明显相关，可能在卵巢癌多药耐药中起重要作用。     

The association of human papillomavirus 16/18 infection with lung cancer among nonsmoking Taiwanese women

Lung cancer is the leading cause of cancer death in Taiwanese women since 1982. High lung cancer mortality ratio of male:female in Taiwan (2:1) was observed, although less than 10% of female lung cancer patients are smokers. Until now, the etiological factor remains unknown. We hypothesize that high-risk human papillomavirus (HPV) 16/18 may be associated with lung cancer development based on high prevalence of p53 negative immunostainings in female lung tumors compared with that of male lung tumors. In this study, 141 lung cancer patients and 60 noncancer control subjects were enrolled to examine whether HPV 16/18 DNA existed in lung tumor and normal tissues by nested PCR and in situ hybridization (ISH), respectively. The concordant detection of HPV 16 and 18 DNA between nested PCR and ISH method was 73 and 85.5%, respectively. Our data showed that 77 (54.6%) of 141 lung tumors had HPV 16/18 DNA compared with 16 (26.7%; P = 0.0005) of 60 noncancer control subjects. In addition, ISH data showed that HPV 16/18 DNA was uniformly located in lung tumor cells, but not in the adjacent nontumor cells. When study subjects were stratified by gender, age, and smoking status, nonsmoking female lung cancer patients who were older than 60 years old had significantly high prevalence of HPV 16/18 infection. The odds ratio of HPV 16/18 infection of nonsmoking female lung cancer patients is much higher at 10.12 (95% confidence interval, 3.88-26.38) compared with 1.98 (95% confidence interval, 0.84-4.76) of nonsmoking male lung cancer patients. This result strongly suggests that HPV infection is associated with lung cancer development of nonsmoking female lung cancer patients. The high prevalence of HPV 16/18 infection may explain to a certain extent why Taiwanese women nonsmokers had a higher lung cancer mortality rate.     

卵巢浆液性肿瘤中OCT4蛋白的表达及与化疗耐药的关系研究

目的:探讨OCT4蛋白在卵巢浆液性肿瘤组织中的表达与卵巢肿瘤发生发展及与化疗耐药的关系。方法:采用免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法（SP法）检测OCT4蛋白在10例正常卵巢组织、10例卵巢良性浆液性囊腺瘤、10例卵巢交界性浆液性囊腺瘤和67例卵巢浆液性腺癌（其中化疗耐药30例,化疗敏感37例）的石蜡病理切片中的表达情况。结果:OCT4蛋白的阳性表达率在正常卵巢组织、卵巢良性浆液性囊腺瘤、卵巢交界性浆液性囊腺瘤和卵巢浆液性腺癌组织中分别为0、40.00%、50.00%和62.69%,差异有显著统计学意义（P=0.002）。在卵巢浆液性腺癌化疗耐药和化疗敏感组织中分别为83.33%和45.95%,差异有显著统计学意义（P=0.002）。OCT4蛋白的阳性表达率与卵巢浆液性腺癌的临床病理特征无明显相关性（P＞0.05）。结论:OCT4蛋白可能在卵巢浆液性肿瘤发生发展和卵巢癌化疗耐药中发挥重要作用,可能作为卵巢癌化疗耐药预测的一个重要参考指标。     

P16抑癌基因在卵巢肿瘤中的表达

 目的:研究抑癌基因P16与卵巢癌的关系, 方法:用免疫组织化学及原位杂交方法检测T72例卵巢肿瘤组织中P16抑癌基因蛋白及其mRNA的表达情况。 结果:免疫组化显示:P16在卵巢癌、良性卵巢肿瘤、交界性肿瘤及正常卵巢组织中的检出率分别为:7.89％、60.00％、6.67％和83.30％, 各组与卵巢癌组相比有显著差异(P<0.01)。 原位杂交表明在上述不同组织中P16mRNA的阳性率分别为21.05％、70.00％、58.33％和75.00％, 各组与卵巢癌组相比有显著差异(P<0.05)。结论:P16在卵巢癌中的表达明显低于其它卵巢肿瘤及正常卵巢组织中, 表明P16作为一种重要的抑癌基因, 它的突变与缺失可能与卵巢癌的发生发展密切相关     

Tenascin-C表达与卵巢上皮性癌分化、浸润及转移的关系

目的:研究细胞外基质(extracellular matrix,ECM)中固生蛋白Tenascin-C (TNC)在卵巢上皮性癌中的表达,探讨各因素对TNC在卵巢上皮性癌中表达的影响.方法:采用免疫组织化学法检测70例上皮性卵巢癌组织、10例卵巢交界组织和10例正常卵巢组织内TNC的表达,观察TNC的表达与上皮性卵巢癌病理学分型、分化程度及临床分期之间的关系.结果:免疫组化结果显示,TNC在上皮性卵巢癌中的阳性表达率高于非卵巢癌组织(P＜0.05).TNC的表达与上皮性卵巢癌的分化程度、临床期别、网膜和淋巴的转移、术前CA125相关(P＜0.05),与年龄、病理类型无关(P＞0.05).结论:TNC在上皮性卵巢癌中高表达,与其分化程度低、临床期别晚、网膜转移阳性、淋巴转移阳性及术前CA125值增高成正相关,而与年龄、病理类型无关.     

应用组织芯片技术研究上皮性卵巢癌组织中VEGF的表达及意义

目的：检测人上皮性卵巢癌组织及卵巢良性肿瘤组织中血管内皮生长因子（vascular endothelial growthfactor,VEGF）的表达,探讨二者在卵巢癌演进和异质化过程中的特点及它们之间的关系及意义。方法：应用组织芯片技术和免疫组织化学染色方法对87例石蜡包埋的人上皮性卵巢癌组织和42例卵巢良性肿瘤组织进行VEGF蛋白检测。结果：在卵巢癌组织中VEGF的高表达率为73.6%,而在卵巢良性肿瘤组织中其高表达率为33.3%,两者差异有显著性统计学意义（P〈0.01）;有淋巴结转移者VEGF的高表达率为86.2%,无淋巴结转移者的高表达率为65.5%,两者VEGF的表达差异有统计学意义（P〈0.01）;在有淋巴结转移的肿瘤中MVD明显高于无转移组肿瘤MVD（P〈0.01）。结论：VEGF高表达与上皮性卵巢癌的发生及其病理特征和生物学行为有密切的关系。VEGF可能是影响上皮性卵巢癌中血管生成的重要因素之一。     

Human papillomavirus infection and papillary squamous cell carcinoma in the head and neck region

Papillary squamous cell carcinoma (PSCC) is a rare variant of SCC in the head and neck region. The role of human papillomavirus (HPV) infection in PSCC is still unclear. We retrospectively reviewed 11 PSCCs in our institute over a 21-year period and compared the HPV status of PSCCs with 26 squamous cell papillomas (SCPs). Polymerase chain reaction (PCR) amplification to detect HPV DNA and in situ hybridization (ISH) were performed to analyze the relationship between the papillary lesions and HPV infection. Immunohistochemical (IHC) staining for p16 protein expression was used to analyze the PSCC specimens. Nine of 11 (82 %), eight of 11 (73 %), and eight of 11 (73 %) PSCC samples were found to be HPV positive by PCR, ISH, and IHC staining for p16 protein expression, respectively. PSCC had a significantly higher rate of HPV infection than SCP by PCR (p?=?0.002) and ISH (p?=?0.001) analysis. This study presents different HPV status in two papillary neoplasms and may help to clarify the unique morphological and biological characteristics of head and neck PSCC.