Effects of Medicinal Cake-Separated Moxibustion on Plasma 6-Keto-PGF1α and TXB2 Contents in the Rabbit of Hyperlipemia

Hyperlipemia rabbit models established with high cholesterol and fat diet were treated with direct moxibustion and medicinal cake-separated moxibustion. The post-treatment plasma 6-keto-prostaglandin F1α (6-keto-PGF1α) and thromboxane B2 (TXB2) contents were determined by radioimmunoassay. Results indicated that the plasma 6-keto-PGF1α content significantly increased, the TXB2 level decreased (P＜0.05)and the TXB2 /6-keto-PGF1α ratio also decreased (P＜0.01) in the medicinal cake-separated moxibustion group as compared with those in the model group respectively, but there was no significant difference between the medicinal cake-separated moxibustion group and the direct moxibustion group (P＞0.05),suggesting that both the medicinal cake-separated moxibustion and direct moxibustion can regulate the plasma 6-keto-PGF1α and TXB2 contents, and the TXB2/6-keto-PGF1α ratio with similar actions, and have a certain protective action on endothelial cells of the aorta in the rabbit of hyperlipemia.     

Changes of thromboxane and prostacyclin in the pathogenesis of multiple organ failure in severe burns

Fifty-seven severely burned patients were divided into 2 groups:16 withmultiple organ failure(MOF),and 41 without MOF.It was found that the levelof thromboxane B_2(TXB_2)and the ratio between TXB_2 and 6-keto-prostaglandinF_(1α)(TBX_2/6-keto-PGF_(1α))in plasma and in visceral tissues were increased and re-mained significantly high in the first 5～7 d postburn in patients with MOF but notso in those without MOF.The circulatory platelet aggregate ratio(CPAR)wasmarkedly decreased in the same period in MOF group.Myocardial enzymes(CPK,LDH,and GOT)were markedly increased in the first 3d and remainedsignificantly high within 7 d postburn.Degeneration,structural destruction,edema,hemorrhage and thrombosis were revealed in cardiac,pulmonary,renal andhepatic tissues succumbing to functional failure.Thirteen out of the 16 cases de-veloped MOF during the 3rd to 7th day posthurn and 11 died in that period.These findings substantiate that persistent increase of thromboxane andthromboxane/prostacyclin ratio is closely related to the origin and development ofMOF after burn injury.     

Interstitial nephritis and interstital nephropathy

930154 Plasma thromboxane B2 and 6-keto-prostaglandin F1 alpha in diabetic nephropathyWANG Yao(王尧),et al,Dept Endocrinol,1stAffil Hosp,Nanjihng Railway Med Coll.210009.Chin J Nephrol 1992;8(5):275-277.The plasma levels of TXB_2 and 6-keto-PGF_1were measured by RIA in 14 diabetics with and49 diabetics without nephropathy and 35 normalsubjects.The results showed that the levels ofplasma TXB_2 and the ratio of TXB_2/6-keto-PGF_.were increased both in diabetics with or     

An experimental and clinical study on TXA_2 and PGI_2 content after head injury

The correlation of secondary brain injury with thromboxane A_2(TXA_2) and prostacy-clin (PGI_2) levels following head injury was studied in rats and patients.Thromboxane B_2(TXB_2) and 6-keto-PGF_(1α) in rat brain homogenate and TXB_2 in cerebral spinal fluid (CSF) ofpatients with severe head injury were determined by RIA.The effects of ligustrazini hydrochlo-rioi were also tested.The results showed that the concentration of TXB_2 and 6-keto-PGF_(1α) inrat brain was progressively increased within 3h after injury (P<0.01),and the ratio betweenTXB_2 and 6-keto-PGF_(1α)(T/K value) increased during the early stage after injury,along withthe tissue damage aggravation.After the use of ligustrazini hydrochlorioi,TXB_2 content inbrain decreased sharply accompanied with a drop in T/K value.Moreover,TXB_2 content inCSF increased within 7 d after injury (P<0.01),and it reached its peak value on the 3rd day.This could indicate that the metabolic imbalance between TXA_2 and PGI_2 might be one of theimportant factors in the development of secondary brain injury,and ligustrazini hydrochlorioiproved to have a protective effect on the brain tissue by normalizing the metabolism of TXA_2and PGI_2.In addition,the metabolic disorders of TXA_2 in the brain tissue of head injured pa-tients have much to do with the outcome of the patients.     

Clinical Implication of the Changes of cAMP, TXA_2 and PGI_2 in CSF of Asphyxiated Newborns

Summary:To evaluate the changes of 3',5'-cyclic adenosine monophosphate(cAMP),thromboxane A_2(TXA_2)and prostacyclin(PGI_2)in cerebrospinal fluid(CSF)in the asphyxiated newborn andexplore their roles in hypoxic-ischamic brain damage(HIBD).Thirty-six full term newborns were di-vided into 3 groups,including 12 with moderate-severe hypoxic-ischaemic encephalopathy(HIE),13with mild HIE,11 without HIE(control group).The levels of cAMP,TXB_2(TXA_2 metabolite)and6-keto-PGF_(1α)(PGI_2 metabolite)in CSF and plasma were measured 36—72h after birth by RIA.andthe concentrations were expressed as nM/L(cAMP),ng/L(TXB_2 and 6-keto-PGF_(1α)).The infantswere followed-up at 6 and 12 month of age and Mental Development Index(MDI)and PsychomotorDevelopment Index(PDI)were measured using Bayley Scales of Infant Development(BSID).TheCSF cAMP level in moderate-severe HIE group was 8.60±2.40,significantly lower than that of themild HIE group(14.83±2.84)and the control group(24.43±2.39)(for both P<0.01).The lev-els of TXB_     

Effects on the Tumor Specific Growth Factor and Tumor Necrosis Factor α in Rats' Precancerous Lesion of Primary Hepatocellular Carcinoma by Direct Moxibustion at Ganshu(BL 18) Acupoint

Objective: To investigate the effect of direct moxibustion at Ganshu(BL18) on the serum concentrations of tumor specific growth factor(TSGF) and tumor necrosis factor α(TNF-α) in a rat model with precancerous lesion of primary hepatocellular carcinoma(HCC), so as to explore the mechanism of moxibustion underlying improvement of HCC. Methods: Sixty male Wistar rats were randomly divided into control group(n=10), model group(n=20), prevention group 1(n=15) and prevention group 2(n=15). The normal rats were injected with physiological saline as blank control. At the same time, the rats of other three groups were injected with diethylnitrosamine to establish the HCC model. Direct moxibustion with grain-sized moxa was applied to bilateral Ganshu acupoint of the rats in the prevention group 1(1 treatment course, 20 days) and prevention group 2(2 treatment courses, 40 days), 5 doses for each acupoint, 0.5 mg/dose, once every other day. At each time point(before model establishment, the end of 1st course prevention, the end of 2nd course prevention and the end of model establishment), serum levels of TSGF and TNF-α were detected using enzyme-linked immunosorbent assay. Results: Compared with the control group, there was a remarkably increase of serum TSGF and TNF-α contents in the model group at the end of the experiment(P0.05). At the end of the 1st course of direct moxibustion, the contents of serum TSGF and TNF-α of rats in the prevention group 1 were significantly increased compared with that of the model group(P0.05). At the end of the 2nd course of direct moxibustion, serum TSGF and TNF-α levels of rats in the model group were higher than the normal group with significantly difference(P0.05), and the levels of TSGF and TNF-α in the prevention group 2 were significantly reduced in comparison with the model group(P0.05). Conclusion: It was possible that direct moxibustion could inhibit precancerous lesion and postpone hepatocarcinogenesis, and the therapeutic effect of two courses were better than one course.     

Effects of the Chinese Drugs for Activating Blood Circulation on Plasma TXB2 and 6-Keto-PGF1α Contents in Rabbits withGlucocorticoid-induced Femoral Head Necrosis

In the femoral head necrosis model rabbit induced by endotoxin combined with methylprenisolone, the dynamical changes of plasma TXB2 and 6-keto-PGF1αcontents were observed. As a result, microscopic examination showed that in the model group, bone trabeculae became fine, empty bone lacunae increased,and osteoblasts in number decreased; the ratio of TXB2 and 6-keto-PGF1α was out of balance, and these changes worsened gradually with the lapse of time; Compound Sheng Mai Cheng Gu Capsules (复方生脉成骨胶囊) could reverse femoral head necrosis, protect the vascular endothelial cells, recover the balance of TXB2 and 6-keto-PGF1α. It is concluded that the hormone-induced femoral head necrosis is closely related with blood stasis, and that the Chinese drugs for activating blood circulation and removing blood stasis can prevent the femoral head from development of necrosis.     

Effects of thromboxane and prostacyclin imbalance on hemodynamics and hemorrheology in severely burn patients

Fifty-seven bum patients were dosdy observed for 28 d postburn.In general,plasma levelof thromboxane B_2(TXB_2),6-keto-PGF_(lx) and TXB_2/6-keto-PGF_(lx) ratio all rose up abruptly tothe peak in the first half day after burns and then declined gradually.However the patterns of theirchanges were different:6-keto-PGF_(lx) returned to the control level in the 2nd day postburn,remained in a higher level than the control even in the 5th day postburn,and the increase ofTXB_2/6-keto-PGF_(lα) ratio was especially pronounced in the first 3 d postbum.It was also shownthat the changes of hemodynamics and hemorrheology occurred simultaneously with the imbalanceof thromboxane and prcstacyclin in the early postburn stage.The extent of the imbalance accordedwith the severity of hemodynamical and hemorrheological changes and was closely correlated withthe changes with the stroke volume,cardiac output,systemic vascular resistance,circulatory plateletaggregate ratio,platelet count and blood vinery.These findings suggest that the imbalance be-tween thromboxane and prostacyclin plays an important role in the changes of hemodynamics andhemorrheology in severe burn cases.     

Clinical study of applying fructose-1,6-diphosphate and captopril to enhance the protective effects of cardioplegia solution on ischemic myocardium

In the present experiment,fructose-1,6-diphosphate(FDP)and captopril(Cap)wereadded to the cold potassium cardioplegia solution and the levels of malondialdehyde(MDA),cre-atine phosphokinase MB(CPK-MB),thromboxane B(TXB_2)and 6-keto-PGF_(1α) in plasma weremeasured during open-heart surgery.Quantitative study of myocardial ultrastructure and obser-vation of cardiac resuscitation were also undertaken.The findings suggested that FDP,especiallywhen combined with Cap could significantly strengthen the protective effects of cold potassiumcardioplegia solution on ischemic myocardium.     

RADIOIMMUNOASSAY OF URINARY TXB2 AND 6-KETO-PGF_(1α) AND ITS CLINICAL SIGNIFICANCE

This article describes a procedure of radioimmunoassay (RIA), which used the acetone deprotein method for pretreatment of urinary samples and for measuring TXB2, 6-keto-PGF_(1α) in urine. It presents the results obtained in 103 normal controls and in various diseases. We find that the controls' values of both U-TXB2 and U-6-keto-PGF_(1α) are normal in distribution. In nephritic group, both of them are not significantly different from controls. In nephrotic group, U-6-keto-PGF_(1α) is increased and U-TXB2 is decreased. In uremic group, both U-TXB2 and U-6-keto-PGF_(1α) are decreased. In primary hypertension group, U-TXB2 is increased and U-6-keto-PGF_(1α) is decreased. In hepatorenal syndrome group, U-TXB2 is very markedly increased. Their clinical significance was discussed.     

Effects of the Chinese Drugs for Activating Blood Circulation on Plasma TXB2 and 6-Keto-PGF1α Contents in Rabbits with Glucocorticoid-induced Femoral Head Necrosis

In the femoral head necrosis model rabbit induced by endotoxin combined with methylprenisolone, the dynamical changes of plasma TXB2 and 6-keto-PGF1αcontents were observed. As a result, microscopic examination showed that in the model group, bone trabeculae became fine, empty bone lacunae increased,and osteoblasts in number decreased; the ratio of TXB2 and 6-keto-PGF1α was out of balance, and these of TXB2 and 6-keto-PGF1α. It is concluded that the hormone-induced femoral head necrosis is closely related with blood stasis, and that the Chinese drugs for activating blood circulation and removing blood stasis can prevent the femoral head from development of necrosis.     

先兆早产患者宫颈粘液6—酮—PGF1α,TXB2变化与硫酸镁疗效的关系

为观察先兆早产患者硫酸镁治疗前后宫颈粘液６－酮－ＰＧＦ１α,ＴＸＢ２变化与疗效的关系,探讨其能否作为判断早产疗效及预后的指标,采用放射免疫法检测２８例先兆早产患者与硫酸镁治疗前后宫颈粘液６－酮－ＰＧＦ１α和ＴＸＢ２水平。结果：治疗成功的患者其宫颈粘膜６－酮－ＰＧＦ１α与ＴＸＢ２水平较治疗前明显下降,６－酮－ＧＰＦ１α与ＴＸＢ２比值明显升高;治疗失败的患者其宫颈粘液６－酮－ＰＧＦ１α和ＴＸＢ２水平较     

隔药饼灸对子宫内膜异位症大鼠雌激素相关因子表达的影响

目的:探索隔药饼灸对子宫内膜异位症大鼠雌激素相关因子表达的影响。方法:采用自体移植法造模,成功后随机将SD大鼠分为模型组、隔药饼灸组和达那唑组,另设未造模的大鼠为空白对照组。空白对照组和模型组大鼠常规固定、暴露关元穴,不做其他处理;其余两组分别给予隔药饼灸法治疗、达那唑悬浊液灌胃,共2个疗程。待治疗结束后,用HE染色观察子宫内膜组织及异位内膜组织形态学变化;ELISA法检测血清E2水平;RT-PCR检测内膜组织芳香化酶(P450arom)和环氧化酶-2(COX-2)的mRNA表达。结果:HE染色结果显示,模型组的异位内膜上皮细胞呈柱状增生,间质细胞密集,局部纤维化,血供丰富;隔药饼灸组的异位内膜上皮细胞萎缩,呈矮立方状,间质细胞萎缩,血供减少,局部呈纤维化。与空白对照组比较,模型组大鼠血清E2水平和异位内膜组织P450arom mRNA、COX-2 mRNA的表达量显著升高(P0.05);与模型组比较,隔药饼灸组和达那唑组大鼠血清E2水平和异位内膜组织P450arom mRNA、COX-2的mRNA表达量明显降低(P0.05);与隔药饼灸组比较,达那唑组血清E2水平和异位内膜组织P450arom mRNA、COX-2 mRNA表达量明显降低(P0.05)。结论:隔药饼灸可能通过改善血液循环,降低子宫内膜异位症大鼠血清E2和异位内膜组织中P450arom、COX-2的表达,从而抑制雌激素的生成代谢、抑制异位病灶的生长。     

Effects of Ligusticum wallichii on the plasma levels of beta-thromboglobulin, platelet factor 4, thromboxane B2 and 6-keto-PGF1 alpha in rabbits under acute experimental cerebral ischemia

By occluding the bilateral carotid arteries of rabbits to produce bilateral partial cerebral ischemia, and by using RIA and ELISA to measure the levels of Beta-thromboglobulin (beta-TG), platelet factor 4 (PF4), thromboxane B2 (TXB2) and 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) in plasma, the authors found that the levels of beta-TG, PF4 and TXB2 in plasma had significantly increased (P less than 0.01), but the level of 6-keto-PGF1 alpha in plasma showed no change (P greater than 0.05) after cerebral ischemia appeared. The results of the Ligusticum wallichii (Ligusticum) pre-treatment to the test-group showed that the levels of beta-TG, PF4 and TXB2 in plasma had significantly decreased (P less than 0.01), and the level of 6-keto-PGF1 alpha in plasma had significantly increased (P less than 0.05). This suggested that the Ligusticum treatment could effectively inhibit the platelet activation in vivo and correct the TXA2-PGI2 imbalance in blood after cerebral ischemia. In this study, some new approaches were explored to explain the mechanisms of Ligusticum for preventing and treating cerebral ischemia.     

参附注射液对脑缺血再灌注大鼠MDA、SOD、TXB2及6-keto-PGF1a的影响及意义?

  目的观察参附注射液对局灶性脑缺血-再灌注大鼠脑组织中超氧化物歧化酶（SOD）活性及丙二醛（MDA）、血栓素B2(TXB2)、6-酮-前列腺素F1a（6-keto-PGF1a）含量的影响,探讨参附注射液对脑的保护机制。方法清洁级雄性SD大鼠60只,随机分为3组假手术组（Sham组,n=20）、脑缺血再灌注组（IR组,n=20）、参附注射液预处理组（SFI组,n=20）。采用线栓法制备大鼠大脑中动脉闭塞后再灌注(MCAOR) 模型, 观察大鼠MCAOR 时神经功能状态,TTC 染色测脑梗死面积, 同时测定血浆中MDA、SOD、TXB2、6-keto-PGF1a的变化。结果参附注射液能有效改善MCAOR大鼠神经功能缺失症状,明显减小梗死灶。IR组与假手术组比较,血清中SOD活性降低,MDA、TXB2含量增加,6-keto-PGF1a含量降低（P<0.05）;参附治疗组与IR组比较,SOD活性增高,MDA、TXB2含量下降,6-keto-PGF1a含量增高（P<0.05）。结论参附注射液对脑缺血再灌注损伤引发的自由基损伤有保护作用,并且能够纠正TXB2/6-keto-PGF1a平衡,达到对脑缺血再灌注大鼠的脑保护作用。     

大鼠脑缺血再灌注后川芎嗪对其血浆TXB2及6-Keto-PGF1α含量的影响

目的本实验旨在揭示大鼠脑缺血再灌注后川芎嗪对其血浆血栓素B2（TXB2）及6-酮-前列腺素F1α（6-Keto-PGF1α）含量的影响。方法线栓大鼠左侧大脑中动脉制作大鼠脑缺血再灌注模型,15只健康sD大鼠随机分为假手术组、模型组和川芎嗪组。观察川芎嗪治疗7天后血浆TXB2和6-Keto-PGF1α含量的变化。结果模型组术后血浆TXB2含量和TXB2／6-Keto-PGF1α比值明显高于假手术组（P〈0.01）。经川芎嗪治疗后,血浆TXB2含量和TXB2／6-Keto-PGF1α明显降低（P〈0.01）,与假手术组比较无统计学差异（P〉0.05）。结论川芎嗪能通过降低血浆TXB2及TXB2／6-Keto-PGF1α达到对脑缺血再灌注大鼠的神经保护作用。     

PGI2,TXA2与先兆早产的关系研究

为探讨前列环酸（ＰＧＩ２）和血栓素（ＴＸＡ２）与先兆早产的关系，采用放射免疫法分别测定了先兆早产和足月临产孕妇血及宫颈粘液中６－酮－ＰＧＦ１α、ＴＸＢ２和孕酮的浓度，并与相对应孕周的孕妇进行比较。结果：与对照组相比，先兆早产组及足月临产组的血浆ＴＸＢ２和６－酮－ＰＧＦ１α水平明显升高（Ｐ〈０．００１），６－酮－ＰＧＦ１α／ＴＸＢ２比值降低（Ｐ〈０．００１），而孕酮水平则无显著性差异（Ｐ〉０．０５）     

Effects of nifedipine and Paeonia lactiflora on plasma TXB2 and 6-Keto-PGF1 alpha in cholesterol-fed rabbits

The authors examined the influences of nifedipine and Paeonia lactiflora (PL) on plasma LPO, TXB2 and 6-keto-PGF1 alpha in cholesterol-fed rabbits. In this study, oral administration of nifedipine (15 mg/kg per day) and PL (0.5 g/kg per day) with 2% cholesterol diet for 15 weeks caused 60.75% and 74.24% reduction in the lesion area of aorta respectively. The levels of plasma LPO, TXB2, cholesterol, phospholipid and calcium of the intimalmedia of the aorta in the treated groups were significantly lower than those in the control group, but the level of 6-keto-PGF1 alpha in the treated groups was significantly higher. The durations of TXB2 elevation and 6-keto-PGF1 alpha reduction were delayed. The ratio of TXB2/6-keto-PGF1 alpha tended to balance. The ratio of TXB2/6-keto-PGF1 alpha was significantly positive correlation with the percentage of lesion area of the aorta. It is demonstrated that calcium metabolism plays an important role in thromboxane, prostaglandin, and LPO synthesis. In conclusion, the inhibition of LPO production and the regulation of TXA2-PGI2 balance may be one of the mechanisms of anti-atherogenesis of calcium antagonists and PL.     

灯盏花素治疗重症急性胰腺炎临床研究

目的：观察灯盏花素治疗重症急性胰腺炎（Severe acute pancreatitis,SAP）的作用。方法：将40例SAP患者随机分为A组（20例）和B组（20例）,同时以健康志愿者20例作为正常对照组。A组采用常规治疗,B组采用常规治疗＋灯盏花素注射液治疗,以放射免疫法测定血浆ET、TXB2、6-keto-PGF1α浓度及TXB2/6-keto-PGF1α比值,测定A、B两组APACHEⅡ评分及胰腺Balthazar CT分级评分。结果：（1）血浆6-keto-PGF1α、ET、TXB2及TXB2/6-keto-PGF1α比值：SAP患者血浆6-keto-PGF1α浓度显著低于正常对照组,而血浆ET、TXB2浓度及TXB2/6-keto-PGF1α比值显著高于正常对照组（P〈0.01）;B组入院后8天与B组入院时及A组入院后8天比较,血浆6-keto-PGF1α浓度显著上升,而血浆ET、TXB2浓度及TXB2/6-keto-PGF1α比值,均显著下降（P〈0.01～0.05）。（2）APACHE II评分和胰腺Balthazar CT分级评分：A、B两组入院后8天与入院时比较,两评分均显著下降（P〈0.01～0.05）;B组入院后8天与A组入院后8天比较,两评分均显著降低（P〈0.01～0.05）。结论：灯盏花素可能通过改善SAP患者的微循环障碍,从而对SAP起治疗作用。