抗心磷脂抗体与偏头痛的关系

采用酶联免疫吸附法（ＥＬＩＳＡ）检测８９例偏头痛患者和６２例健康人血清中ＩｇＧ、ＩｇＡ和ＩｇＭ３种类型抗心磷脂抗体（ＡＣＬ）。结果表明患者组３种类型ＡＣＬ指数均显著高于对照组（Ｐ＜０．０５，Ｐ＜０．０１），其中３５例具有一种类型以上ＡＣＬ阳性（３９．３％），以ＩｇＧ型阳性为主。而且女性患者ＡＣＬ阳性率显著高于男性。提示偏头痛的发病与ＡＣＬ生成有一定关系。     

抗心磷脂抗体与偏头痛的关系

用ＥＬＩＳＡ法分别检测６５例偏头痛患者和健康人血清中ＩｇＧ，ＩｇＭ型抗心磷脂抗体（ＡＣＬ）结果偏头痛组ＡＣＬ阳性率显著高于对照组（Ｐ〈０．０１），另外还发现ＡＣＬ阳性的偏头痛患者有性别差异，提示偏头痛的发病与ＡＣＬ生成有关系。     

84例脑梗塞病人抗心磷脂抗体的研究

８４例脑梗塞病人和 ５０例对照者进行了抗心磷脂抗体（Ａｎｔｉｃａｒｄｉｏｌｉｐｉｎ ａｎｔｉｂｏｄｉｅｓ，ＡＣＡ）检测，结果发现。脑梗塞病人组２２例ＩｇＧ—ＡＣＡ和ＩｇＭ－ＡＣＡ滴度均高于正常人（Ｐ分别＜０．０１、０．０５），脑梗塞组ＡＣＡ阳性率为２６％，其中ＩｇＧ—ＡＣＡ阳性１２例、ＩｇＧ和ＩｇＭ—ＡＣＡ均为阳性６例，ＩｇＭ—ＡＣＡ阳性４例，提示ＡＣＡ与脑梗塞的发病有一定关系。     

抗心磷脂抗体与偏头痛关系的研究

用ＥＬＩＳＡ法分别对７５例偏头痛患者和正常人测定血清抗心磷脂抗体（ＡＣＡ），阳性率分别为３２％和２．６７％，差异显著（Ｐ＜０．０１）；另外还发现ＡＣＡ阳性的偏头痛患者无性别差异。结果说明ＡＣＡ与偏头痛有关，并对ＡＣＡ激发偏头痛的发病机制做了探讨。     

抗心磷磷抗体与脑血管病相关性研究

目的：研究抗心磷脂抗体（ＡＣＡ）与脑血管病的关系，为脑卒中的预测提供临床依据。方法：采用前瞻性研究方法，对９１例脑梗死（ＣＩ）、４２例脑出血（ＣＨ）患者及３０例健康者血清中的抗心磷脂抗体（ＡＣＡ）进行检测。结果：ＣＩ及ＣＨ组ＡＣＡ总阳性率、ＩｇＧ型ＡＣＡ阳性率均显著高于对照组。ＡＣＡ阳性卒中容易复发，病灶常为多个。ＡＣＡ在脑卒中发病一周内阳性率最高，且抗体分型与ＩｇＧ型为主。结论：ＡＣＡ是ＣＩ及Ｃ     

复发性脑梗塞与抗心磷脂抗体,血脂关系的研究

目的探讨复发性脑梗塞与抗心磷脂抗体、血脂的关系。方法采用酶联免疫吸附（ＥＬＩＳＡ）等方法检测了３７例复发性脑梗塞（ＲＣＩ）和６８例首发性脑梗塞（ＦＣＩ）患者的血清抗心磷脂抗体（ＡＣＬＡ）及血脂７项指标，并与５０例对照组比较。结果ＲＣＩ组的ＡＣＬＡ阳性率明显高于ＦＣＩ及对照组；ＴＧ、ＴＣ、ＬＤＬ－ｃ及ＡＰＯＢ血清平均水平ＲＣＩ和ＦＣＩ组均明显高于对照组，而血脂水平两组比较差异无统计学意义。结论ＡＣＬＡ与ＲＣＩ的发生有密切关系，血脂代谢对ＲＣＩ和ＦＣＩ的影响无差别。     

抗心磷脂抗体与脑卒中关系的研究及其临床意义

报告用ＥＬＩＳＡ法对脑血栓１０４例、ＴＩＡ和脑供血不足６８例，脑出血３３例，进行了ＡＣＡ检测，并与１００例健康者相对照。结果发现，脑血栓组ＡＣＡ阳性率为３９．４％，脑出血组阳性率为１５．１％，ＴＩＡ和脑供血不足组阳性率为８．８％，对照组为６％，揭示脑血栓组与对照组比较有极显著性差异（Ｐ（０．０１）。在脑血栓组中，测定显示ＡＣＡ中ＩｇＧ型比ＩｇＭ型和ＩｇＡ型阳性率为高（９２％对１７％和１４％），提示ＩｇＧ与临床密切有关。另外，还发现ＡＣＡ阳性与青壮年卒中关系更为密切，且ＡＣＡ阳性者的脑卒中以多发性梗塞更为多见，结果说明，ＡＣＡ可做为脑梗塞危险性增加的指标。     

血管性痴呆患者31例血清抗磷脂抗体水平的测定

抗磷脂抗体 (ａｎｔｉｐｈｏｓｐｈｏｌｉｐｉｄａｎｔｉｂｏｄｙ ,ａＰＬ)是循环血清中的多克隆免疫球蛋白 (ＩｇＧ、ＩｇＭ、ＩｇＡ或其复合物 )。它包括抗心肌磷脂抗体 (ａｎｔｉｃａｒｄｉｏｌｉｐｉｎａｎｔｉｂｏｄｙ ,ａＣＬ)和狼疮抗凝物(ｌｕｐｕｓａｎｔｉｃｏａｇｕｌａｎｔ,ＬＡ)两大类。关于抗磷脂抗体与血管性痴呆的关系 ,目前报道尚不多见。我们检测了 31例血管性痴呆患者的血清ａＣＬ ,以探讨ａＣＬ与血管性痴呆的关系。资料 :分组 :(1)血管性痴呆 (ｖａｓｃｕｌａｒｄｅｍｅｎｔｉａ ,ＶＤ)组 :31例 ,男 2 0例 ,女 11例 ,…     

中风病人抗心磷脂抗体的测定与临床研究

报告用ＥＬＩＳＡ法对２０５例脑卒中及脑供血不足病人，进行了抗心磷脂抗体（ＡＣＡ）检测，并与１００例健康者相对照。结果发现，脑血栓组ＡＣＡ阳性率为３９．４％，脑出血组阳性率为１５．１％，ＴＩＡ与脑供血不足组阳性率为８．８％，对照组６％，揭示脑血栓组与对照组比较有极显著性差异（Ｐ＜０．０１）。在脑血栓组中测定显示ＡＣＡ中ＩｇＧ型比ＩｇＡ型阳性率为高（９２％对１７％和１４％），提示ＩｇＧ与临床密切有关。另外，还发现ＡＣＡ阳性与青壮年卒中关系更为密切，且以多发性梗塞更为多见。结果说明ＡＣＡ可做为脑梗塞危险性增加的指标。     

中青年脑梗塞患者的抗磷脂抗体研究

以酶联免疫吸附法对８５例脑梗塞患者（＜５０岁３７例，≥５０岁４８例），２１例非缺血性脑血管病患者及１４名正常人的血清抗心肌磷脂抗体（ａＣＬ）进行测定，结果＜５０岁和≥５０岁脑梗塞患者的ａＣＬ阳性率均明显高于对照组（均Ｐ＜０．０５）；而＜５０岁脑梗塞患者的ａＣＬ阳性率明显高于≥５０岁患者（Ｐ＜０．０５）。提示临床上无任何自身免疫疾病的中青年脑梗塞患者与ａＣＬ相关更为密切。     

Migraine and headache in systemic lupus erythematosus and their relationship with antibodies against phospholipids

Summary It has been reported that migraine is common in systemic lupus erythematous (SLE) and an association with phospholipid antibodies has been suggested. The incidence of migraine and non-migrainous headache was prospectively studied in 90 patients with SLE and 90 age-and sex-matched controls. A history of migraine was commoner in SLE patients than in controls [31(34%) vs 15(16%);P<0.05], and the mean age of onset was higher in the SLE group (26.8 vs 17.2 years). Within the SLE group an association was found between migraine and SLE disease activity. Non-migrainous headaches were also more common (non-significant) in the SLE group, and there was a close temporal relationship between onset of both headache and SLE in many patients. Both migraine and non-migrainous headaches in SLE patients often responded to specific SLE treatment. No association was found between migraine or other headaches and antibodies to phospholipids.     

Possible pathogenic link between migraine and urotensin-II

Our aim was to determine the levels of human urotensin-II (hU-II) in the plasma of migraine patients and controls, to ascertain if there were a difference in the pathogenesis of migraine. A total of 27 patients who suffer from migraines and 27 controls were included in the study. Venous blood samples were drawn twice both from migraine patients and controls to measure hU-II plasma levels. The average levels of hU-II during migraine episode, between episodes, and controls were found to be 0.483, 0.493, and 0.737 pg/mL, respectively. The levels of hU-II in the controls were higher significantly. When comparisons were made according to sex, age groups, and types and durations of migraine, there was no significant difference in the levels of hU-II in the patients. The low levels of hU-II in the plasma of migraine patients compared with controls may be an indicator of its role in the pathogenesis.     

Occurrence of factor V Leiden mutation (Arg506Gln) and anticardiolipin antibodies in migraine patients

The occurrences of factor V Leiden mutation (Arg506Gln) and antiphospholipid antibodies (APA) in migraine patients have been reported, but the findings are controversial. We investigated the presence of factor V Leiden and the serum level of anticardiolipin antibodies (aCL) in a consecutive series of 70 migraine patients (47 women; mean age, 34.1 years). Of these, 40 patients had migraine with aura. A matched sample of 70 healthy people was considered as the control group. Heterozygous genotype for factor V Leiden mutation was detected in 4 (5.7%) migraine patients (of which 2 had migraine with aura) and in 2 (2.8%) subjects of the control group. Although proportionally more migraine patients harbored the factor V Leiden mutation, this difference was not statistically significant, perhaps due to the small number of patients involved. We found normal serum levels of aCL in all migraine patients. Further studies and a long-term follow-up are warranted to determine the significance of this genetic abnormality in migraine. Received: 5 October 2002 / Accepted in revised form: 22 January 2002     

Brain MRI white matter lesions in migraine patients: is there a relationship with antiphospholipid antibodies and coagulation parameters?

Brain magnetic resonance imaging (MRI) studies in migraine patients have demonstrated lesions consisting of focal regions of increased signal intensity within the white matter. Antiphospholipid antibodies are known to have a role in many diseases including migraine. The aim of the present study was to ascertain the relationship between MRI-visualized cerebral focal hyperintense lesions and serum antiphospholipid antibody levels, as well as blood coagulation parameters in migraine patients. One hundred and two (77 females, 25 males, mean age 33.8 ± 11.1) consecutive migraine patients and a control group of 94 (70 females, 24 males, mean age 33.2 ± 10.8) healthy subjects were enrolled. All individuals underwent brain MRI. Complete blood examinations, autoantibodies, antiphospholipids antibodies including anticardiolipin and lupus anticoagulant (aCL, LAC), antithrombin III, Protein C and S serum levels were ascertained in the subjects who presented white matter lesions on MRI. Twenty-seven (26.4%) migraine patients and six (6.3%) healthy subjects in the control group showed focal regions of increased intensity signal within cerebral white matter (odds ratio 5.3, 95% CI: 1.98–16.36). In migraine patients with white matter lesions, antiphospholipid antibodies were not detected and serum levels of antithrombin III, and proteins C and S were normal. White matter lesions in migraine patients are fairly common. This finding is not associated with antiphospholipid antibodies or abnormal coagulation parameters. The significance of such lesions at present remains unclear.     

Catecholamine levels in plasma and CSF in migraine.

There is clinical and pharmacological evidence of the existence of sympathetic dysfunction in migraine. Adrenaline and noradrenaline concentrations were determined in plasma and CSF of patients during attacks of common or classic migraine, comparing them with controls suffering from stress. Plasma noradrenaline levels were significantly lower in the patients with common migraine than in controls (p < 0.05). Other catecholamine levels in plasma and CSF in both migraine groups were only slightly lower than in controls. Our results suggest that central sympathetic dysfunction exists in patients with migraine.     

偏头痛、抗磷脂抗体与脑梗塞三者关系的临床研究

目的 :研究和探讨偏头痛与缺血性卒中的相关性以及抗磷脂抗体在偏头痛相关性卒中中的作用。方法 :运用问卷调查方式统计普通人群及脑梗塞患者偏头痛的患病率 ,将脑梗塞患者按有否偏头痛病史分为两组 ,用 EL ISA方法分别测定其血清抗心磷脂抗体 (ACA)水平。结果 :(1)脑梗塞患者偏头痛的患病率与普通人群偏头痛的患病率分别为 2 0 %和 5 .95 % ,二者之间有显著性差异 (P<0 .0 5 )。 (2 )脑梗塞伴有偏头痛史者和无偏头痛史者 ,其 ACA阳性率分别为 4 1.6 7%和 14 .5 8% ,二者具有显著性差异 (P<0 .0 5 )。结论 :偏头痛病史与脑梗塞具有明显相关性。抗磷脂抗体可能参与偏头痛所相关的脑梗塞的发病机制     

Neuroexcitatory plasma amino acids are elevated in migraine

To investigate the role of glutamic (Glu) and aspartic acid (Asp) in migraine, we measured the plasma amino acids in migraine patients with and without aura, between and during attacks, and compared the profiles with the plasma amino acid profiles of tension headache patients and healthy controls. Between attacks, migraineurs (notably with aura) had substantially higher plasma Glu and Asp levels than did controls and tension headache patients. In addition, patients with migraine without aura showed low plasma histidine levels. During migraine attacks, Glu (and to a lesser extent Asp) levels were even further increased. The results suggest a defective cellular reuptake mechanism for Glu and Asp in migraineurs, and we hypothesize a similar defect at the neuronal/glial cell level, predisposing the brain of migraineurs to develop spreading depression.     

The importance of cytokines, chemokines and nitric oxide in pathophysiology of migraine

The certain etiology migraine is unknown. The study was aimed at determining to the efficiency of cytokines, chemokines and nitric oxide (NO) to the pathophysiology of migraine. The levels of cytokines, chemokines and NO in serum of 25 patients with migraine during attacks and attack-free periods and 25 healthy controls were investigated. The levels of cytokines and chemokines were determined by enzyme-linked immunosorbent assay. NO concentrations were determined by a nitrate/nitrite colorimetric assay kit. In attack groups, IL-10 levels were found higher than in attack-free groups and healthy controls (p<0.05). IL-6 levels in migraine patients were significantly higher than in healthy controls. The levels of RANTES were high in attacks groups. There was an increase NO concentrations in migraine attacks. The study's results reflect that the etiology of migraine is multifactorial and probably related to immunological changes.     

Increased fibrinogen,d-dimer and galectin-3 levels in patients with migraine

There are limited studies evaluating the fibrinogen levels in patients with migraine. It remains unknown whether the levels of the haematological marker of thromboembolism, d-dimer, and the levels of galectin-3, which plays an important role in inflammation as a proinflammatory mediator, change during the attacks in patients with migraine. The present study aims to compare galectin-3, fibrinogen and d-dimer levels in patients with migraine during the attacks and interictal periods, and to compare galectin-3, fibrinogen and d-dimer levels between patients with migraine and healthy controls to investigate the role of these parameters in the pathogenesis of migraine. Fifty-nine patients with migraine and 30 age-gender matched healthy control subjects were enrolled in the study. Blood galectin-3, fibrinogen and d-dimer levels were measured in patients with migraine. Patients with migraine had higher levels of galectin-3, fibrinogen and d-dimer compared to the healthy controls (p < 0.05). No statistically significant difference was found between galectin-3 and fibrinogen levels during the attacks and interictal period in the migraine group (p > 0.05). Migraine patients had higher d-dimer levels during the attacks compared to the patients in the interictal period in the migraine group (p = 0.05). In conclusion, we found increased levels of fibrinogen, d-dimer and galectin-3 in patients with migraine compared to the healthy control group. Furthermore, we showed increased galectin-3 levels in patients with migraine, and higher d-dimer levels during migraine attacks compared to the interictal periods for the first time. These findings may be associated with the hypercoagulability and neurogenic inflammation during migraine headaches.