Clinical Application of Moxibustion on Guanyuan （CV 4）

Guanyuan （CV 4） is located 3 cun below the umbilicus, on the midline of abdomen. It is the Front-Mu Point of the Small Intestine, a crossing point of Three Yin Meridians of Foot and Conception Vessel. It can warm the kidney to invigorate yang, tonify the Yuan-Primordial qi, dredge and regulate Thoroughfare and Conception Vessels,     

Clinical Study on Treatment of Primary Dysmenorrhea with Qihuang Herbal Warm Bag

目的观察"歧黄"养生温灸包对原发性痛经患者的影响.方法以原发性痛经患者32例为观察对象,采用温灸包外敷的方法,治疗2个月经周期后观察临床疗效,并检测患者治疗前后的前列腺素F2a(PGF2a)及β内啡肽(β-EP)的变化.结果温灸包对不同程度的痛经患者均有疗效,可使血中PGF2a含量下降及β-EP升高.结论温灸包治疗原发性痛经有效.温灸包能够降低血清PGF2a含量,增加血清β-EP含量.     

Effects of Hegu （LI 4） and Taichong （LR 3） on Vascular Dilation and Constriction in Migraine Patients

Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmonize yin and yang, regulate qi and blood, and balance ascending or descending. This paper attempts to study the effects of Si Guan point on the vascular dilation and constriction in migraine patients from the perspective of its feature.     

Risk of Ovarian Cancer in Women with Pelvic Inflammatory Disease： A Population-based Study

Background: Ovarian cancer is commonly fatal and incidence has persistently risen in Taiwan over the past 20 years. Prevention strategies, however, are limited. Pelvic inflammatory disease (PID) has been suggested to increase the risk of developing ovarian cancer, but the     

Effect of a comprehensive therapy plus Gushenyutai plaster admin- istered at Guanyuan （CV 4） on male infertility associated with se- men non-liquefaction

OBJECTIVE： To observe the clinical effect of Gushenyutai plaster administered at the Guanyuan（CV4） acupoint on male infertility associated with semen non-liquefaction.METHODS： Sixty-two male patients with infertility caused by semen non-liquefaction were random-ized into a treatment and control group. The control group received comprehensive therapy, which included oral administration of clarithromycin sustained release tablets, hip bath with Zhongyaoxiaoyan granules, prostate massage, and transurethral microwave treatment. The treatment group was administered Gushenyutai plaster in addition to the comprehensive therapy. Both groups were treated for 8 weeks. After treatment, the clinical effect and pregnancy rate were assessed and compared between the two groups.RESULTS： The effective rate of the treatment group was significantly higher than that of the control group（96.77% vs 70.97%, P〈0.05） and the pregnancy rates of the treatment group and control groups were 38.71% and 16.13%, respectively（P〈0.05）.CONCLUSION： The effect of Gushenyutai plaster plus comprehensive therapy was better than that of the comprehensive therapy alone on male infertility induced by semen non-liquefaction.     

Comparative Study on the Effect of Baihui （GV 20）, Shuigou （GV 26） and Shenmen （HT 7） on Cognition of Patients with Vascular Dementia

目的:探讨百会、水沟、神门改善血管性痴呆(VD)患者认知功能的相对特异性.方法:50例血管性痴呆患者,随机分为5组,分别治以常规穴位针刺,常规穴位加用百会穴,加用水沟穴,加用神门穴,加用百会、水沟和神门3穴.治疗前后运用老年认知功能量表(SECF)进行测评.结果:常规穴位加用百会、水沟、神门等穴位治疗后,SECF总分有显著提高;加用百会穴治疗后,VD患者的定向、瞬间记忆、长回忆、动物名、划销和计算、分类与类同等方面评分有明显提高;加用水沟穴治疗后,VD患者的定向、划销和计算、分类与类同等方面评分有明显提高;加用神门穴治疗后,VD患者的数字广度、动物名、分类与类同等方面评分有明显提高.结论:百会、水沟、神门能够改善VD患者认知功能,三穴虽然分别有各自的相对特异性,但联合运用效果最佳.     

Effect of Pressed Moxibustion at Baihui （GV 20） on Thromboxane A2 and Prostacyclin in Treating Vertebral Artery Type of Cervical Spondylosis

Aim: To explore the curative ettect ot pressed moxibustion mainly at the Baihui (GV 20) on vertebral artery type of cervical spondylosis (CSA). Methods: Totally 34 CSA patients were randomly divided into two groups: treatment group (n=17) and control group (n=17). All the patients were treated with local electroacupuncture, and besides, those in the treatment group received pressed moxibustion at Baihui (GV20), The clinical curative effects were observed,     

Effect of Acupuncture at Acupoints Neiguan （PC 6） and Ximen （PC 4） on the Left Cardiac Function in the Patients with Coronary Heart Disease

目的：探讨针刺内关与郄门穴对冠心病患者每博心输出量及左心室排血的峰值流速的影响。方法：60例冠心痛患者分为内关组和郄门组，分别在运针3min后和留针20min后测试每博心输出量，左心室排血的峰值流速。再与针刺前得测试结果相比较。结果：46例患者针刺3min后Q值和VP值较针刺前上升。留针20min后，34例患者Q值和VP值全部上升。结论：针刺内关和郄门可增强左心室壁的收缩能力，使心脏的每博输出量增加，改善冠心病患者的心肌缺血状态。     

基于代谢组学探讨艾灸关元穴对老年大鼠肾能量代谢的影响＊

目的 通过UHPLC-Q-TOF-MS代谢组学探讨艾灸关元穴对老年大鼠肾代谢物的影响，进而为艾灸关元穴的作用机制提供参考。方法 将8月龄SD雄性大鼠设为成年对照组（8只），21月龄SD雄性大鼠随机分为老年对照组（8只）、老年金匮肾气丸组（7只）、老年艾灸组（8只）。老年金匮肾气丸组每日按体重给药，老年艾灸组每日艾灸关元穴15 min，均每周5天。实验持续13周后检测大鼠肾组织线粒体呼吸耗氧速率、琥珀酸脱氢酶（SDH）活性以及血清肾功能指标，观察肾脏病理变化，结合UHPLC-Q-TOF-MS技术对大鼠的肾组织进行代谢轮廓分析，筛选代谢差异物并进行鉴定。结果 与老年对照组比较，老年艾灸组大鼠肾线粒体的呼吸耗氧速率和SDH酶的活力显著提高（P<0.01）。代谢组学结果显示，肾组织中筛选出13个共同差异化合物，分别是丁酸十二烷基酯、亚油酰胺、5-甲基四氢叶酸、PC（16∶0/22∶5（7Z，10Z，13Z，16Z，19Z））、6，8-二羟基嘌呤、1，2，3-丙烷三羧酸、3-（4-甲氧基苯基）-2-氧代丙酸、吲哚-3-乙酰甘氨酸、亚麻油酸、9，10-环氧十八烷酸、二十二碳五烯酸（22n-6）、牛磺胆酸、LysoPS （18∶0/0∶0）。结论 艾灸关元穴可通过调控老年大鼠的牛磺酸和亚牛磺酸代谢、α-亚麻酸代谢、亚油酸代谢、甘油磷脂代谢来调节肾的能量代谢。     

运用fMRI观察电针关元及中脘穴相对特异性的脑功能网络

目的:比较电针关元和中脘穴激活的脑功能区及其功能区网络连接的异同。方法:21名健康志愿者参加电针关元穴、中脘穴时的fMRI配对试验,在针刺前静息状态、留针、电针(15 Hz,3次30 s,间以1 min)、拔针后静息态采集fMRI脑功能数据,并记录电针任务后的针感。采用t检验及卡方检验分析针感,SPM 2、短程和长程"中心度"法分析脑激活功能区及其之间的功能网络连接。结果:关元穴显示更强的胀满感。电针两穴均产生了额叶内侧部的明显负激活,留针及电针能改变脑静息默认功能模式,额叶内侧回、扣带前回前下部及海马区(脑边缘叶-旁边缘叶-新皮层系统)脑功能网络出现了明显的即时针刺后效应。两穴位脑效应存在较小的差异。结论:针刺关元、中脘穴均具有对脑边缘叶-旁边缘叶-新皮层网络的调制作用,但也存在较小的差异,显示了穴位脑效应相对特异性。     

现行《玉龙歌》中关元穴之误辨析

本文通过考查历代医籍关于《玉龙歌》及对关元穴相关内容的记载,发现了千百年来《玉龙歌》中关元穴记载的失误。现行《玉龙歌》中"关元兼刺大敦穴"句中的关元穴实非任脉上的关元穴,应是经外奇穴阑门穴。其失误原因主要是由于字形相近而致误抄作"閣門"関门"蘭門"闗元"閉悶"。     

针刺足三里、关元穴对脓毒症大鼠胸腺细胞凋亡的影响

目的 观察电针足三里、关元穴对脓毒症大鼠胸腺细胞凋亡的影响.方法 将大鼠随机分成正常对照组、模型组、针刺组.采用盲肠结扎穿孔(CLP)致腹腔感染脓毒症模型,应用流式细胞仪以Annexin-PI双染色法及光镜、透射电镜观察CLP致腹腔感染后36h胸腺淋巴细胞的凋亡情况,应用放射免疫法测定脑垂体和外周血血管活性肠肽(VIP)含量的变化.结果 CLP致腹腔感染可导致胸腺细胞凋亡增多,电镜和光镜显示其典型的形态学特征,模型组、针刺组的胸腺细胞凋亡率高于正常对照组;而针刺组的胸腺细胞凋亡率低于模型组.CLP致腹腔感染后脑垂体和外周血中VIP含量下降,模型组、针刺组脑垂体VIP含量低于正常对照组;而针刺组脑垂体VIF-含量高于模型组.模型组、针刺组外周血中VIP含量低于正常对照组;而针刺组外周血中VIP含量高于模型组.结论 电针足三里、关元穴可以减轻CLP致腹腔感染脓毒症大鼠胸腺细胞凋亡,其机理可能与VIP的合成和释放增多有关,并通过这些免疫递质对神经-内分泌-免疫调节网络发挥作用.     

近5年敏化态与非敏化态关元穴临床主治规律的文献计量学分析＊

目的 总结近5年来临床随机对照试验（RCTs）中敏化态与非敏化态关元穴的主治范围与规律。方法 应用计算机检索中国期刊全文数据库（CNKI）、维普数据库（VIP）、中国生物医学光盘数据库（CBM）、万方数据库（WanFang Data）以及PubMed数据库，筛选出符合纳入标准的文献，以文献计量学的方法对纳入文献进行统计，归纳和分析出两种状态关元穴的主治疾病和治疗相关情况。结果 非敏化态关元穴共纳入370篇文献，其文献发表数量呈现先增加再逐渐减少的趋势，治疗的疾病有121种，尿潴留和原发性痛经文献数量最多，其次为多囊卵巢综合征、失眠症等，共纳入15707例患者；作用于关元穴的治疗方式有20种，以单纯针刺和艾灸为主；针刺角度以直刺为主，深度多在1-1.5寸之间，平补平泻法和补法是其常用补泻方法；常见病治疗频次主要为1次/日，不同疾病的配穴和疗程各有不同。敏化态关元穴共纳入24篇文献，文献发表数量趋势无明显变化，主治疾病有16种，共纳入919例患者，治疗方式主要为热敏灸，以热敏化形式为主。结论 非敏化态关元穴的主治疾病范围较广，且治疗方式多样，具体治疗时间、配穴和疗程因疾病种类不同而有所区别；敏化态关元穴有较好的临床疗效，但研究较少，有待进一步探索。     

电针正常人足三里和关元穴中枢效应的fMRI脑功能成像比较

目的:利用fMRI脑功能成像,比较电针时位于肢体的足三里穴及腹部的关元穴激活的脑功能区及其功能区网络连接的异同。方法:21名健康志愿者参加电针足三里穴、关元穴时的fMRI试验。分别在针刺前静息状态、留针、电针(15Hz,3次30s,间隔1min)、拔针后静息态采集fMRI脑功能数据,并记录电针任务时针感。采用t检验及卡方检验分析针感,SPM 2、短程和长程"中心度"法分析脑激活功能区及其之间的功能网络连接。结果:在电针电流无显著性差异条件下,电针足三里穴较关元穴诱导出了更强的胀满感及麻木感。电针两穴均产生了相似的前下扣带回-额叶内侧回网络的显著负激活效应,留针及电针能改变脑静息态脑功能默认功能模式,于额叶内侧回、扣带前回前下部短程脑功能网络出现了明显的即时针刺后效应。足三里穴较关元穴产生了更强的短程脑功能网络连接。结论:针刺足三里、关元穴具有显著相似的对脑边缘叶-额叶内侧皮层脑网络的调制作用,但也存在小的差异,体现穴位脑效应的相对特异性。     

Antioxidant effect of indirect moxibustion on healthy subjects: a pilot study

Objective

Moxibustion has long been thought to promote vital energy or immunity through clinical experience. This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulation in a normal population.

Methods

Twenty-five healthy volunteers with no objective or subjective disorders were recruited. The participants were treated with indirect moxibustion on acupoints Guanyuan (CV 4) and Shenque (CV 8) three times per week for 4 weeks. The serum levels of reactive oxygen species (ROS) and malondialdehyde (MDA), the total antioxidant capacity (TAC), the activities of catalase and superoxide dismutase (SOD), and the total glutathione content were determined before and after the 12th moxibustion. Lymphocyte subpopulations and 42 cytokines in the peripheral blood were analyzed using flow cytometry and antibody array, respectively.

Results

Compared with the initial time point, the serum levels of ROS and MDA were significantly lowered by moxibustion, while TAC was increased (P<0.01 for all). A significant increase was observed in catalase activity (P<0.05), but not in SOD or total glutathione. There were no significant changes in lymphocyte subpopulations or cytokines in the peripheral blood. Fifteen of 25 participants reported at least one symptom in which they felt subjective improvement after moxibustion.

Conclusion

Indirect moxibustion on acupoints CV 4 and CV 8 improved the antioxidant defense system, which may be a mechanism explaining the clinical effects of moxibustion.     

Observation of influence of mild moxibustion by different intervals on therapeutic effects of patients with primary hyperlipidemia

目的：观察不同间隔时间温和灸对原发性高脂血症患者疗效的影响。方法：将63例患者随机分为A组（32例）和B组（31例。两组均采用温和灸神阙和双侧足三里穴,A组每天灸治1次,B组隔天灸治1次,12星期后统计两组疗效。结果：两组对降低总胆固醇（totalcholesterol,TC）有极其显著性意义（P〈0．001）;降低甘油三酯（triglyceride,TG）、低密度脂蛋白胆固醇（low densitylipoproteincholesterin,LDL—C）和升高高密度脂蛋白胆固醇（highdensitylipoproteincholesterin,HDL—C）都有显著性意义（P〈0．05）;对载脂蛋白AI（apolipoproteinAI,ApoAI）、载脂蛋白B（apolipoproteinB,ApoB）和脂蛋白[lipoproteina,Lp（a）]的改善不明显,但apoAI有较明显的升高趋势,同样apoB和Lp（a）也有较明显的降低趋势。组间比较没有统计学意义。结论：温和灸治疗原发性高脂血症可以选用隔日1次的方案。     

艾灸"神阙"穴对肾阳虚家兔血清SOD的影响

目的:探讨艾灸神阙穴对衰老的影响和作用机制.方法:将48只健康家兔随机分成8组,即Ⅰ组(空白对照组)、Ⅱ组(模型组)、Ⅲ组(神阅预防组)、Ⅳ组(神阙加足三里预防组)、Ⅴ组(神阙加命门预防组)、Ⅵ组(神阙治疗组)、Ⅶ组(神阙加足三里治疗组)、Ⅷ组(神阙加命门治疗组).采用羟基脲片灌胃法对后7组进行肾阳虚家兔造模,从预防及治疗两方面观察艾灸对各组家兔血清超氧化物歧化酶(SOD)活性的影响.通过单用"神阙"与"神阙"加"足三里"、"神阙"加"命门"等穴位组合进行比较,观察不同穴位配伍对以上指标影响的差异.结果:造模后,Ⅱ、Ⅵ、Ⅶ、Ⅷ组出现明显的肾阳虚症状,SOD活性降低(均P<0.01);经艾灸治疗后,Ⅵ、Ⅶ、Ⅷ组肾阳虚症状明显改善(均P<0.01),出现明显的SOD活性升高(均P<0.01);Ⅲ、Ⅳ、Ⅴ组造模后出现少许肾阳虚症状,造模前后SOD活性改变不明显(均P>0.05);艾灸"神阙"、"神阙"加"足三里"、"神阙"加"命门"3种方法组间比较差异不明显(均P>0.05).结论:艾灸"神阙"、"神阙"加"足三里"、"神阙"加"命门"3种穴位配伍均可以有效调节肾阳虚家兔血清SOD的活性,对肾阳虚家兔具有明显的预防及治疗作用;单用"神阙"穴和"神阙"加其他穴位相比,其作用差异不明显,艾灸"神阙"穴延缓衰老的机制与调节血清SOD活性关系密切.