胃癌及肠上皮化生组织bcl—2,c—erbB—2和p53表达及其临床意义

目的 研究ｂｃｌ－２,ｃ－ｅｒｂＢ－２,ｐ５３蛋白表达在胃癌发生中的作用。方法 免疫组织化学方法。结果 ｂｃｌ－２,ｃ－ｅｒｂＢ－２,ｐ５３蛋白表达阳性率分别为６０％,２２％和３８％。３种蛋白表达之间无显著相关性,且与胃癌临床病理参数亦无显著相关性。肠化组织ｂｃｌ－２表达阳性率为５３．３％。结论三种蛋白在胃癌的发生中可能起重要作用,检测３种蛋白表达可能有助于预测胃粘膜癌变。     

胃癌p53和c—erbB—2基因产物的表达及其与病理临床的相关性研究

用免疫组化ＡＢＣ法对９２例胃癌的石蜡包埋标本进行了ｐ５３和ｃ－ｅｒｂＢ－２基因蛋白表达的研究。结果显示：９２例胃癌ｐ５３、ｃ－ｅｒｂＢ－２蛋白表达的阳性率分别为３８．０％和１４．１％；ｐ５３表达主要位于细胞核内，而ｃ－ｅｒｂＢ－２表达主要位于细胞膜，ｐ５３及ｃ－ｅｒｂＢ－２表达与胃癌患者的性别、年龄、原发灶部位、淋巴结及远处转移、肿瘤分期以及病理类型、分级均无明显关系。ｐ５３阳性表达的胃癌患者生存期明显低于ｐ５３阴性者，而ｃ－ｅｒｂＢ－２表达与胃癌患者的生存期无关；ｐ５３与ｃ－ｅｒｂＢ－２二者阳性表达之间亦无关。５例ｐ５３及ｃ－ｅｒｂＢ－２表达的阳性者预后极差，提示，癌基因的异常表达对预后的估计具有辅助意义。     

中期胃癌nm23、p21和c-erbB-2基因的表达及其临床意义

目的：探讨ｎｍ２３、ｐ２１和ｃ－ｅｒｂＢ－２基因在中期胃癌的表达及其临床意义。方法：应用免疫组化（ＳＰ）方法，检测手术切除标本癌组织中ｎｍ２３、ｐ２１和ｃ－ｅｒｂＢ－２的表达。结果：（１）６９例中期胃癌标本中的ｎｍ２３低表达、ｐ２１及ｃ－ｅｒｂＢ－２过表达率分别为７１．０％（４９／６９）、６０．９％（４２／６９）及６６．７％（４６／６９）；（２）无淋巴结转移及Ⅰ＋Ⅱ期胃癌的ｎｍ２３表达率（８７．５     

乳腺乳头状瘤病及其癌变组织c-erbB-2及p53蛋白表达的免疫组化研究

目的探讨ｃ－ｅｒｂＢ－２及ｐ５３蛋白在乳腺乳头状瘤病及其癌变过程不同发展阶段的表达水平及其对早期癌变的诊断价值。方法对乳腺乳头状瘤病及其癌变不同发展阶段手术标本存档蜡块１８４例进行ｃ－ｅｒｂＢ－２及ｐ５３蛋白免疫组化检测。结果乳头状瘤病自轻度、中度、重度至癌变整个过程，均有不同程度的ｃ－ｅｒｂＢ－２蛋白表达，阳性率逐渐升高，分别为４．８％、２５．０％、３２．４％及５０．０％。ｐ５３蛋白在乳头状瘤病自轻度至重度均无表达，至乳头状瘤病癌变只出现少数表达，阳性率３．５％，而至浸润性癌达高峰，阳性率为２５．０％。结论ｃ－ｅｒｂＢ－２蛋白表达对早期发现重度乳头状瘤病癌变具有重要参考价值。ｐ５３蛋白在这些病变中，未见或甚少表达，对乳头状瘤病早期癌变不具诊断参考意义。     

膀胱癌多基因和增殖细胞核抗原的表达及其临床意义

目的：研究癌基因和抑癌基因蛋白产物在膀胱移行细胞癌组织中的异常表达与其病理分级、临床分期的关系。方法应用免疫组织化学方法检测９６例膀胱移行细胞癌中ｐ５３、ｎｍ２３、ｂｃｌ－２、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２和ＰＣＮＡ的表达水平。结果９６例膀胱移行细胞癌中ｐ５３、ｎｍ２３、ｂｃｌ－２、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２和ＰＣＮＡ的阳性表达率分别为６０．４％、７２．３％、６８．８％、８１．３％、６６．７％和     

胃癌内镜活检组织p53,c—erb—B2和nm23蛋白检测的临床意义

胃癌内镜活检组织ｐ５３、ｃ┐ｅｒｂ┐Ｂ２和ｎｍ２３蛋白检测的临床意义魏子白吴云林翟祖康王瑞年关键词胃肿瘤内窥镜术基因产物，ｐ５３ｃ┐ｅｒｂ┐Ｂ２基因产物，ｎｍ２３胃癌临床分期的确定一直依赖于术中探查和术后病理检查，这就限制了合理治疗方案的选择，因此应...     

胃粘膜异型增生和癌的c—erbB—2,p53癌基因产物的表达

对６５例胃粘膜异型增生和５４例胃癌进行免疫组化研究，结果显示Ⅰ级，Ⅲ级异型增生无ｃ－ｅｒｂＢ－２和ｐ５３蛋白表达，Ⅲ级中表达ｃ－ｅｒｂＢ－２７例，ｐ５３８例，胃癌中ｃ－ｅｒｂＢ－２阳性Ⅰ级６０．０％，Ⅱ级４２．９，Ⅲ级２５．０，Ⅰ－Ⅱ级癌的ｃ－ｅｒｂＢ－２阳性率与Ⅲ级比较差异有显著性，ｐ５３慢白阳性Ⅰ级９０．０％，Ⅱ级５０．０％，Ⅲ级４５．０％，Ⅰ－Ⅱ级癌ｐ５３蛋白阳性率与Ⅲ级比较差异有显著性。     

乳腺癌组织蛋白酶D,c—erbB—2蛋白,nm23蛋白表达与淋巴结转？…

目的 探讨乳腺癌组织蛋白酶Ｄ（ＣａｔｈｅｐｓｉｎＤ）,ｃ－ｅｒｂＢ－２３,ｎｍ２３的表达与淋巴结转移的关系。方法 应用免疫组织化学Ｓ－Ｐ法,检测１０８例乳腺癌Ｃａｔｈ－Ｄ、ｃ－ｅｒｂＢ－２、ｎｍ２３的表达情况。结果 Ｃａｔｈ－Ｄ、ｃ－ｅｒｂＢ－２、ｎｍ２３阳性表达率分别为５８．３％、５３．６％,４８．１％。Ｃａｔｈ－Ｄ,ｃ－ｅｒｂＢ－２阳性表达率与乳腺癌腋窝淋巴结转移之间呈正相关（Ｐ〈０．０５）,     

c—erbB—2,p53,p21基因在乳腺癌,胃癌和大肠癌中过度表达的临床 …

对９０例乳腺癌、胃癌和大肠癌同时检测癌肿同及转移淋巴结的ｃ－ｅｒｂＢ－２、ｐ５３、ｐ２１的过度表达。结果显示乳腺癌、胃癌和大肠癌转移淋巴中ｃ－ｅｒｂＢ－２、ｐ５３及ｐ２１的阳性率分别与原发癌肿内的阳性率之间无显著性差异（Ｐ〉０．０５）。转移淋巴结与原发癌肿之间ｃ－ｅｒｂＢ－２、ｐ５３及ｐ２１过度表达与否的平均符合率分别为７３．１％、６５．４％、７３．１％。显示转移淋巴结中基因过度表达与否同原发癌肿     

c-erbB-2、p53、p21基因在乳腺癌、胃癌和大肠癌中过度表达的临床意义

对９０例乳腺癌、胃癌和大肠癌同时检测癌肿内及转移淋巴结的ｃ－ｅｒｂＢ－２、ｐ５３、ｐ２１的过度表达。结果显示乳腺癌、胃癌和大肠癌转移淋巴中ｃ－ｅｒｂＢ－２、ｐ５３及ｐ２１的阳性率分别与原发癌肿内的阳性率之间无显著性差异（Ｐ＞０．０５）。转移淋巴结与原发癌肿之间ｃ－ｅｒｂＢ－２、ｐ５３及ｐ２１过度表达与否的平均符合率分别为７３．１％、６５．４％、７３．１％。显示转移淋巴结中基因过度表达与否同原发癌肿基本一致。在原发癌肿内ｃ－ｅｒｂＢ－２、ｐ５３及ｐ２１基因的过度表达（除乳腺癌中的ｐ２１表达）分别与癌肿的预后有一定关系，但在不同癌肿中所表示的预后指标有较大差异。９０例中多种基因蛋白过度表达占５２．２％，且提示上述癌肿预后较差。同时检测ｃ－ｅｒｂＢ－２、ｐ５３和ｐ２１过度表达对癌肿的预后判断明显优于单个基因蛋白的检测。     

Prognostic significance of p53, nm23, PCNA and c-erbB-2 in gastric cancer

BACKGROUND: Although the TNM stage is the most important prognostic factor for gastric cancer, there is a need for new prognostic and predictive factors, because the prognosis varies among patients of the same stage. The purpose of this study was to clarify the relationship of p53, nm23, proliferating cell nuclear antigen (PCNA) and c-erbB-2 with the clinicopathological parameters and the survival results. METHODS: For 841 patients who had undergone gastrectomy for gastric cancer at Seoul National University Hospital from July 1996 to December 1997, the expression levels of p53, nm23, PCNA and c-erbB-2 in gastric cancer tissues were examined immunohistochemically. Also, the clinicopathological parameters such as gender, age, operation type, TNM stage and size of the tumor, histology and Lauren classification were analyzed retrospectively. RESULTS: There were 568 males and 273 females (2.07:1) with a mean age of 56 years (range:25-82 years). The percentages of positive expression of p53, nm23 and c-erbB-2 were 43, 74 and 17%, respectively; 59% of tumors expressed PCNA index > or =50. p53 expression was associated with age, gender, tumor size, histology, Lauren classification, stage, nm23 expression, PCNA index >or =50 and c-erbB-2 expression. nm23 expression was associated with age, tumor size, Borrmann type, histology, Lauren classification and stage. PCNA index > or =50 was associated with age, gender, tumor size, Borrmann type, histology, Lauren classification and c-erbB-2 expression. c-erbB-2 expression was associated with gender, Borrmann type, histology and Lauren classification. p53 and nm23 were related with poor prognosis in univariate analysis. nm23 was related with poor prognosis of stage III and diffuse-type gastric cancer in univariate subgroup analysis. However, in a multivariate study, these prognostic impacts were not maintained. CONCLUSION: The expression of p53 and nm23 seems to be related with poor prognosis of gastric cancer patients who have undergone gastrectomy. However, the prognostic significance was not revealed by a multivariate analysis.     

The clinical significance of tumor angiogenesis and invasiveness-related gene expressions in gastric cancer

Gastric cancer is a malignant tumor with the highest morbidity and mortality in China. Despite improvement of surgical treatment, postoperative metastasis remains the leading cause of death. Therefore, searching for biological indicators, which can predict the metastatic potential of gastric cancer, and treating the high risk cases individually have become one of the important subjects in the current research. With the deepening of the research on molecular biology, some biological indicators …     

p53和c-erbB-2表达与胃癌进展的关系

目的探讨p53和c-erbB-2表达与胃癌进展的关系。方法收集139例胃癌手术切除标本,包括黏膜内癌23例、浸润至黏膜下层31例、浸润至肌层47例、浸润至浆膜或浆膜外38例,切片采用免疫组化S-P法染色。结果进展期胃癌中p53和c-erbB-2阳性表达率均明显高于早期胃癌（P值分别为0.010、0.000）。c-erbB-2表达与p53表达存在相关性（P=0.023〈0.05）。c-erbB-2阳性表达是胃癌发展的1个危险因素,p53表达阳性和阴性的OR值比较无统计学意义（P〉0.05）。结论抑癌基因p53突变有利于癌基因c-erbB-2的表达;c-erbB-2阳性表达是胃癌发展的1个危险因素,与胃癌的发展密切相关。     

nm23蛋白与C—erbB—2蛋白在乳腺癌中的表达及临床意义

目的：探讨乳腺癌中抑制转移基因ｎｍ２３的表达及其与Ｃ－ｅｒｂＢ－２、ＥＲ、ＰＲ以及肿瘤组织学分级、淋巴结转移等临床病理特性的相互关系。方法：应用免疫组化ＳＰ法检测７０例乳腺癌中ｎｍ２３蛋白、ＣｅｒｂＢ－２蛋白、ＥＲ、ＰＲ的表达。结果：乳腺癌中ｎｍ２３蛋白阳性率为６２．８６％,ｎｍ２３蛋白的表达与组织学分级、细胞核分级、淋巴结转移以及临床分期均有相关性（Ｐ〈０．０１）,但与Ｃ－ｅｒｂＢ－２蛋白、ＥＲ     

胃癌组织中p53、c-erbB-2、EGFR、nm23、E-cadherin基因表达及预后价值

寻找胃癌临床病理特征和预后的可靠性分子指标,建立一种能被临床普遍应用的简易方法。方法：应用免疫组化法检测了具有随 １７１例胃癌标本中ｐ５３、ｎｍ２３、ｃ－ｅｒｂＢ－２、ＥＧＦＲ和Ｅ－钙粘蛋白（Ｅ－ｃａｄｈｅｒｉｎ,简写为Ｅ－ｃａｄ）的表达。结果：（１）Ｃｏｘ比例风险模型多因素分析表明,ｐ５３,ｃ－ｅｒｂＢ－２,ＥＧＦＲ和Ｅ－ｃａｄ是影响胃癌预后的因子;ｐ５３、ｃ－ｅｒｂＢ－２及ＥＧＦＲ表达的胃癌病     

Usefulness and limitation of multiple endoscopic biopsy sampling for epidermal growth factor receptor and c-erbB-2 testing in patients with gastric adenocarcinoma

BACKGROUND: Our objective was to examine the utility of endoscopic biopsy specimens in judging the status of epidermal growth factor receptor (EGFR) and c-erbB-2 genes and proteins in the entire tumor. METHODS: Endoscopic biopsy specimens and specimens of whole representative cut surfaces of corresponding surgically resected tumors were obtained from 14 patients with gastric carcinoma, and immunohistochemistry and fluorescence in situ hybridization were then performed to determine the protein expression and gene amplification profiles, respectively, of EGFR and c-erbB-2 in these biopsy and surgical specimens. RESULTS: Among the eight endoscopic biopsy specimens obtained from three gastric carcinomas in which EGFR protein overexpression and gene amplification were judged to be positive in the corresponding surgically resected tissue specimens, EGFR overexpression was detected in three specimens (38%), but EGFR amplification was not detected (0%). Among the 19 endoscopic biopsy specimens obtained from five gastric carcinomas in which c-erbB-2 protein overexpression and gene amplification were judged to be positive in the corresponding surgically resected tissue specimens, c-erbB-2 overexpression and amplification (c-erbB-2/CEP17 ratio) were detected in 14 (74%) and 16 (84%) specimens, respectively. All three cases with EGFR overexpression and all five cases with c-erbB-2 overexpression showed intratumor heterogeneity with regard to their EGFR and c-erbB-2 status, respectively. CONCLUSIONS: The c-erbB-2 status could be adequately assessed not only by examining surgically resected materials, but also by examining multiple endoscopic biopsy specimens. On the other hand, to assess the EGFR status accurately, the use of surgically resected samples appeared to be more reliable than the use of multiple endoscopic biopsy samples.     

原发性输尿管癌p53、c-erbB-2和nm23表达与临床病理特征的关系

目的　研究输尿管癌 p5 3、c- erb B- 2、nm2 3基因的不同表达与其临床病理因素的关系。方法　应用免疫组化 S- P法检测 p5 3、c- erb B- 2、nm2 3等基因蛋白在 2 4例原发性输尿管癌中的表达情况 ,并对其结果与临床病理各参数的关系进行分析。结果　正常输尿管上皮三种基因蛋白均呈阴性 ,在输尿管癌中 p5 3、c- erb B- 2、nm2 3的阳性表达率分别为 41.6 % (10 /2 4)、5 4.1% (13/2 4)和 6 6 .7% (16 /2 4)。 p5 3、c- erb B- 2高表达与输尿管癌分化程度低、浸润至浆膜或浆膜外、生存期短等几项临床病理特征相关。nm2 3的表达与输尿管癌病理分级、浸润深度、临床预后无密切关联。结论　 p5 3、c- erb B- 2和 nm2 3基因参与了输尿管癌的发生发展过程 ,p5 3、c- erb B- 2蛋白表达可以作为输尿管癌生物学行为和预后判断的参考指标 ,两指标同时检测预示作用更可靠     

Oncogenes in male breast cancer

The objective of this study was to assess the degree of expression and prognostic significance of c-erbB-2, p53, and bcl-2 in male breast cancer (MBC). Thirty male patients with the diagnosis of adenocarcinoma of the breast were studied retrospectively. All patients underwent surgery; c-erbB-2, p53, and bcl-2 were immunohistochemically stained on sections from formalin-fixed, paraffin-embedded tissues. Seventeen (56.7%) of the 30 cases of MBC were bcl-2 positive. Few specimens were found positive for c-erbB-2 (6.7%) and p53 (6.7%). The 5-year survival rate was marginally better for those patients with tumors staining positively for bcl-2 (p = 0.05). It was impossible to estimate the association between survival rate and p53 and c-erbB-2 expression because of the small number of positively stained specimens. In this study, only bcl-2 showed marginal association to other tumor parameters and a trend toward a better 5-year survival rate. At present there is inadequate evidence to support the use of molecular markers as independent prognostic markers in MBC.     

胃癌和癌前病变中幽门螺杆菌感染和增殖细胞核抗原,p53,c—erbB— …

目的 探讨幽门螺杆菌感染在胃癌发生发展中的作用。方法 对７０胃癌、６２例癌前病变和１６例对照组,应用改良Ｗａｒｔｈｉｎ－ｓｔａｒｒｙ法检测Ｈｐ,免疫酶组化ＳＰ法检测增殖细胞核抗原（ＰＣＮＡ）、ｐ５３、ｃ－ｅｒｂＢ－２等蛋白。结果 ＨＰ杂率在胃癌和癌前病变组均高于对照组,而前两组间差异无显著性,肠型和约漫型胃癌间ＨＰ感染率差异亦无显著性;胃癌和癌前病变组中,ＨＰ感染与ＰＣＮＡ表达、ｃ－ｅｒｂＢ－２过     

乳腺癌组织中nm23、c-erbB-2、p53表达与临床病理特征的关系

目的　研究乳腺癌组织中nm 2 3、c erbB 2、p5 3基因蛋白的表达与临床病理特征的关系。方法　采用免疫组化S -P法 ,分别检测 76例乳腺癌、10例乳腺良性病变组织中nm 2 3、c erbB 2、p5 3基因蛋白的表达。 结果　c erbB 2、p5 3蛋白在乳腺良恶性病变之间的表达率有显著性差异 (P <0 .0 5 ,P <0 .0 1)。nm 2 3、c erbB 2蛋白表达与乳腺癌的组织学分级有关 (P <0 .0 5 ,P <0 .0 1)。 3种基因蛋白的表达均与腋窝淋巴结转移相关 (P <0 .0 1) ,与乳腺癌的组织学类型、患者年龄及肿瘤大小无关 (P >0 .0 5 )。在乳腺癌组织中 ,nm 2 3与c erbB 2、nm 2 3与 p5 3的表达呈均负相关 (P <0 .0 1,P <0 .0 5 ) ,c erbB 2与p5 3的表达则呈正相关 (P <0 .0 5 )。结论　nm 2 3、c erbB 2、p5 3基因参与乳腺癌的发生发展过程。联合检测nm 2 3、c erbB 2、p5 3基因蛋白对预测乳腺癌淋巴结转移及判断预后有重要意义