不同处理方法对胚胎移植受体绵羊同期发情效果的影响

本实验利用CIDR PMSG对绵羊进行同期发情处理。并分别用不同LH剂量对86只受体绵羊进行了诱发排卵试验:方法一100IU,方法二200IU,方法三300IU的。结果表明,CIDR PMSG法同期发情率为:95.3%,第1d的发情率(61%)显著高于第2d的发情率(39%)(P<0.01)。方法一的优质黄体率(55.2%)显著高于方法二的优质率(26.9%)(P<0.05),方法一(55.2%)与方法三(34.6%)之间的优质率差异不显著,但方法一比较节省药品。因此,方法一的效果最好。     

和田羊不同方法同期发情效果比较

本试验于2011年3月进行,选取240只健康的和田羊,利用CIDR＋PG和CIDR＋PMSG对其进行同期发情处理效果的试验。结果显示：实施CIDR＋PG和CIDR＋PMSG同期化处理,同期发情率分别为96.7%和99.2%,情期受胎率分别为85.3%和84.9%,两者之间差异不显著（P〉0.05）。     

非繁殖季节不同品种绵羊同期发情试验研究

在全舍饲条件下,对处于非繁殖季节的56只道赛特羊、86只萨福克羊、158只特克赛尔羊随机分成两组,一组采用CIDR+PMSG同期处理,另一组采用孕酮海绵+PMSG同期处理,并对发情羊及时进行常温人工受精.结果表明:①A组(CIDR+PMSG法)与B组(孕酮海绵栓+PMSG法)两种方法比较,同期发情率差异显著[P<0.05];情期受胎率差异不显著(P>0.05].②A组处理羊中:道赛特羊、萨福克羊、特克赛尔羊同情发情率、情期受胎率差异不显著[P>0.05];B组处理羊中:道赛特羊同期发情率显著高于萨福克样和特克.赛尔样[P<0.05];B组处理羊中,萨福克羊情期受胎率显著高于道赛特羊和特克赛尔羊[P<0.05].③两组方法产羔率差异不显著[P>0.05];A组处理羊中:三品种产羔率差异不显著[P>0.05];B组处理羊中:道赛特羊产羔率显著高于萨福克样和特克赛尔样[P<0.05).     

非繁殖季节绵羊诱导发情试验

对处于非繁殖季节的300只阿勒泰大尾羊采用CIDR PMSG法进行诱导发情处理,结果表明CIDR埋植12d和14d没有显著差异,较大剂量的PMSG可以提高母羊产羔率。     

东北马鹿同期发情技术的研究

试验采用三种发情方案对东北马鹿进行同期发情处理,第一组,采用自制海绵栓加PMSG法处理;第二组,采用新西兰进口CIDR PMSG法处理;第三组,采用PG PG PMSG法处理。本试验中,三种方法处理后的同期发情率,自制海绵栓组为81.8%(36/44)、进口叉栓组92%(46/50)、PG PG组42.1%(8/19),两阴道栓组之间差异不显著(P>0.05),两阴道栓组极显著高于PG PG组。     

辽宁绒山羊同期发情效果的研究

将45只辽宁绒山羊平均分为3个组:A、B、C,按如下程序进行同期发情处理,A:CIDR PMSG FSH PG(CIDR埋植12d);B:CIDR FSH PG(CIDR埋植12d);C:CIDR PMSG FSH PG(CIDR埋植16d)。结果表明:(1)超排时加注250IUPMSG撤栓后48h内的同期发情率显著提高(试验组A:86.7%VS试验组B:73.3%,P<0.05);(2)撤栓后48h内的同期发情率埋栓16d组高于埋栓12d组,但两组间差异不显著(试验组A:86.7%VS试验组C:93.3%,P>0.05)。     

胚胎移植技术在纯种德国美利奴羊选育中的应用研究

本研究对 2 8只纯种德国肉用美利奴供体羊进行超排处理 ,供体羊共计排卵 453枚 ,平均每只羊 1 6 .1 8± 4.0 4枚 ,共回收可用胚胎 385枚 ,平均每只羊 1 3.75± 4.0 1枚。将 385枚胚胎移植给 32 6只受体羊 ,产羔率达到 77.6 1 %。结果表明 :①供体羊的年龄对超数排卵效果没有明显的影响 ;对性成熟不久的供体羊 ,初次诱发排卵时适当降低FSH剂量可与成年经产母羊一样有较好的超排效果。排卵点分别为1 6 .86± 4.6 1个 (育成羊 )、1 5.6± 3.40个 (成年羊 ) ,可用胚 1 4.1 5± 4.45枚 (育成羊 )、1 3.4± 3.56枚 (成年羊 ) ;②合理的饲养管理是受体羊胚胎移植后受胎率的关键 ,补饲条件下受体羊产羔率 ( 81 % )显著高于典型草原放牧型受体羊的产羔率 ( 76 .1 % ) ;③供受体羊的发情同步差对受体羊的产羔率有重要的影响 ,同步差 0h、± 1 2h的受体羊产羔率 ( 76 .54 % )显著高于同步差± 2 4h的产羔率 ( 52 .54 % )。     

非繁殖季节绵羊同期发情试验

为评估绵羊在非繁殖季节同期发情效果,近三年来,我们连续在绵羊的非繁殖季节,利用同期发情、人工授精技术,对绵羊繁殖生产进行了调控试验,并与同年度繁殖季节绵羊同期发情繁殖效果进行了对照比较.结果对指导今后绵羊秋冬羔生产和高频繁殖具有重要意义.     

用孕马血清促性腺激素处理的多胎羊排卵率、胚胎成活率和血浆孕酮含量

由47头和51头Finnish Landrace×Dorset Horn母羊组成两组,在用500国际单位孕马血清(PMSG)处理后D组和A组分别在12月6日和4月8日同期发情时以Suffolk公羊配种。妊娠50—145天将母羊屠宰。D组和A组母羊的排卵率分别为4.5±0.24和5.0±0.32。这些母羊均为诱发发情,它们相应的羔羊数为2.8±0.15和2.8±0.18。在早期妊娠(P<0.001)和晚期妊娠(P<0.05)期间,D组外周血浆中的孕酮平均含量高于A组。排卵率的增加(达到4个或5个)与羔羊平均数的增加有关。     

高寒牧区柯尔克孜羊同期发情和人工授精试验

为了提高柯尔克孜羊的繁殖率,本试验选择处于繁殖季节的600只柯尔克孜羊,随机分为试验组和对照组,试验组母羊采用一次PG方法处理;对照组为母羊自然发情,不做任何处理。观察第一情期的发情情况和发情后卵巢状态,统计同期发情率、受胎率和产羔率。结果显示:试验组同期发情率60%(120/200)显著高于对照组20%(80/400),差异显著(P0.05)。试验和对照组处理后发情羊的卵巢状态相同,试验组和对照组受胎率分别为75%和78.7%,差异均不显著(P0.05),产羔率分别为94.4%和95.2%,差异均不显著(P0.05)结论:通过在高寒牧区使用一次PG法对处于繁殖季节的柯尔克孜羊进行处理不仅达到促进母羊发情同期化程度、集中进行人工授精的目的,而且取得了预期的同期发情率、受胎率及产羔率,值得广泛推广。     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.