电子麻醉对电刺激诱发猫二腹肌动作电位的影响

目的：判断ＹＤＭ－ １ 牙科电子麻醉仪是否通过中枢发挥镇痛作用。方法：电刺激猫尖牙和下牙槽神经干，观察二腹肌肌电的变化。结果：ＹＤＭ－ １ 牙科电子麻醉仪可以同时抑制电刺激牙齿和下牙槽神经干诱发的二腹肌肌电的电位。结论：ＹＤＭ－１ 牙科电子麻醉仪可以通过中枢发挥镇痛作用。     

YDM—1牙科电子麻醉仪诱发Vc内Fos表达

目的：探讨面部ＴＥＮＳ后,三叉神经脊束核尾侧亚核（Ｖｃ） 内兴奋细胞的分布。方法：利用免疫组织染色化学方法观察正常以及面部ＴＥＮＳ后１ 、２、４ｈ 大鼠Ｖｃ 内Ｆｏｓ 免疫阳性神经元的分布。结果：ＴＥＮＳ后１ｈ,Ｆｏｓ 免疫阳性神经元集中分布在Ｖｃ 浅层背侧区,ＴＥＮＳ后２ｈ ,内层也出现少量Ｆｏｓ 免疫阳性神经元,ＴＥＮＳ后４ｈ ,Ｖｃ 各层内可见Ｆｏｓ 免疫阳性神经元的分布。结论：Ｖｃ 浅层和内层的神经元均参与ＴＥＮＳ反应,早期主要是Ｖｃ 浅层背侧区神经元。     

经皮穴位电刺激对不可逆性牙髓炎患者下牙槽神经阻滞的影响

目的 评估应用经皮穴位电刺激(TEAS)对下颌磨牙不可逆性牙髓炎行开髓治疗时下牙槽神经阻滞(IANB)成功率、疼痛及焦虑状况的影响.方法 将2020年10月至2021年1月来自徐州市中心医院口腔科的48例因不可逆性牙髓炎需行开髓治疗患者使用Stata 12.0按1:1比例随机分为实验组(24例)和对照组(24例),将组...     

rhBMP-2和FGF-2对成骨细胞矿化及ENPP1、ANK、TNAP表达的影响

目的:研究重组人骨形态发生蛋白(rhBMP-2)和重组人碱性成纤维细胞生长因子(FGF-2)对成骨细胞(MC3T3-E1 Subclone 14)矿化及焦磷酸合成酶(ENPP1)、跨膜蛋白(ANK)和组织非特异性碱性磷酸酶(TNAP)表达的影响,探讨生长因子影响细胞矿化的机制。方法:将MC3T3-E1 Subclone 14细胞分成3组:成骨细胞诱导液(OS)成骨诱导培养组(对照组),OS与rhBMP-2培养组(rhBMP-2组),OS与FGF-2培养组(FGF-2组)。培养12 d后进行ALP活性检测及茜素红染色,实时荧光定量PCR检测矿化相关基因ENPP1、ANK和TNAP表达的差异。结果:rhBMP-2组ALP活性以及钙化结节明显高于对照组,ENPP1、ANK和TNAP均高表达;FGF-2组ALP活性以及钙化结节明显低于对照组,ENPP1和ANK呈高表达,TNAP低表达。结论:rhBMP-2和FGF-2通过调节ENPP1、ANK和TNAP的表达变化来影响骨的矿化。     

超前镇痛对阻生牙微创拔牙术后镇痛效果及MDAS评分的影响

目的：探讨超前镇痛对阻生牙微创拔牙术后镇痛效果及改良牙科焦虑量表（Modified Dental Anxiety Scale,MDAS）评分的影响。方法：选择2021年7月—2022年5月南通市如东中医院收治的阻生牙患者100例,采用随机数字表法分为试验组（微创拔牙术联合超前镇痛治疗,n=50）和对照组（微创拔牙术,n=50）。比较2组患者手术时间、术中血压和心率、术后疼痛视觉模拟量表评分（VAS评分）、术后MDAS评分和不良反应。采用SPSS 22.0软件包对数据进行统计学分析。结果：试验组手术时间显著少于对照组（P<0.05）,术后5、10和24 h疼痛VAS评分显著低于对照组（P<0.05）;2组治疗后MDAS评分均低于治疗前,且试验组显著低于对照组（P<0.05）;2组治疗后不良反应发生率均较低,无显著差异（P>0.05）。结论：超前镇痛对阻生牙微创拔牙术后镇痛效果较好,可改善患者焦虑状况,且不良反应少,值得推广使用。     

影响自体牙移植的相关因素分析

目的:探讨年龄、性别、是否根管治疗、术区应用浓缩生长因子(CGF)及受牙区牙位等因素对自体牙移植的成功率及存活率的影响.方法:收集2016年1月～2018年1月行自体牙移植73例患者临床资料,分析自体牙移植成功率的影响因素,采用χ2检验和Logistic回归分析移植牙的3年累积存活率和成功率.结果:73例自体牙移植患者...     

增龄因素对鼠正畸牙牙周组织骨保护素及其配体表达的影响

目的:比较幼年鼠和成年鼠在正畸牙移动中牙周组织骨保护素(OPG)及其配体(OPGL)表达的不同比值,探讨增龄因素对正畸骨改建影响的分子机制。方法:制备大鼠正畸牙齿移动模型,于牙齿移动1d、3d、5d、7d、10d、14d及14d后去除正畸力1周后取材,免疫组化检测牙周组织OPG和OPGL的表达。结果:①.牙齿移动3d时,幼年鼠压力侧OPGL的表达明显增强;而成年鼠此时OPGL的表达没有幼年鼠明显。②.牙齿移动5d和7d时,幼年鼠和成年鼠OPGL的表达均成强阳性,破骨细胞多。③.牙齿移动10d、14d时,幼年鼠和成年鼠OPGL的表达逐渐减弱。④.14d时去除正畸力至21d时发现幼年鼠和成年鼠OPGL均已成弱阳性表达,幼年鼠可见部分成骨细胞。结论:在正畸力的作用下,OPGL与OPG的表达比值与年龄关系密切,增龄因素引起的牙周组织内OPGL/OPG表达变化可能是导致成年正畸特点的分子机制之一。     

增龄因素对鼠牙周组织骨保护素表达的影响

目的 比较幼年鼠和成年鼠牙周组织骨保护素(Osteoprotegerin,OPG)信使RNA(mRNA)表达的不同,以探讨增龄因素影响牙周组织改建的分子机制。方法 以4周(幼年鼠)和24周(成年鼠)雄性大鼠右上第一磨牙的牙周膜和邻近牙槽骨为实验对象,利用OPG多相寡核苷酸探针原位杂交检测实验部位OPG mRNA的表达。结果 1.幼年鼠近牙周膜的牙槽骨面可见破骨细胞和相应的骨陷窝;2.成年鼠牙周膜内细胞的OPG mRNA表达高于幼年鼠;3.鼠牙槽骨组织中,成年鼠和幼年鼠骨髓腔内均有OPGmRNA表达阳性的细胞,但成年鼠中的表达明显高于幼年鼠。结论 增龄因素使牙周组织内OPG表达明显增强,提示幼年的牙周组织较成年的牙周组织有较强的骨改建能力。     

种植体骨结合稳定性测量及相关因素分析

目的:测量种植体骨结合稳定系数(ISQ),分析影响稳定性的相关因素。方法:对39名种植术后3~6个月后要进行修复的患者(男25,女14),102枚ITI种植体骨结合稳定性进行共振频率(RFA)测量。结果:ITI种植体骨结合ISQ为:76.29±5.25;ITI种植体稳定性主要受种植体骨质影响,密质骨(I、II)种植体稳定性(78.73±3.5)要高于松质骨(III、IV)的稳定性(74.27±5.63)(P<0.05);上颌与下颌的种植体稳定性差异不明显(P>0.05),上颌后牙区稳定性(77.57)要高于前牙区(73.65,P<0.05),下颌前后牙区无明显差别(P>0.05);不同长度不同直径种植体之间稳定性无明显差异(P>0.05)。结论:种植体稳定性与种植体周围骨质关系密切,种植体ISQ可以协助判断上部结构的修复时机,缩短种植修复的周期。     

PRF 及所含三因子对大鼠脂肪干细胞增殖和黏附的影响

目的：研究富血小板纤维蛋白（PRF）及其所含三因子 TGF-β1、PDGF-AB、VEGF 分别对大鼠脂肪干细胞（ADSCs）增殖和黏附的影响。方法：将 ADSCs 培养在含有 PRF 膜和不同浓度的 PDFG-AB、TGF-β1、BEGF 中,细胞黏附实验检测培养2 h 后细胞黏附能力,CCK-8法检测培养1～7 d 细胞增殖。结果：黏附实验显示,PRF 组的黏附细胞数显著高于阴性对照组（P ＜0．05）;不同浓度的 PDGF-AB 组内黏附细胞数的差异无统计学意义（P ＞0．05）;不同浓度的 TGF-β1、VEGF 组内黏附细胞数的差异有统计学意义（P ＜0．05）。CCK-8实验表明,PRF 组的细胞增殖在各个时间点（1、3、5、7 d）显著高于阴性对照组（P ＜0．05）;不同浓度的 PDGF-AB、VEGF 组内细胞的增殖差异有显著性（P ＜0．05）;不同浓度的 TGF-β1组细胞增殖的差异无显著性（P ＞0．05）。结论：PRF 能提高 ADSCs 的增殖和黏附的能力。PDGF-AB 和 VEGF 可促进 ADSCs 的增殖;TGF-β1和 VEGF 均可促进 ADSCs 的黏附,并呈一定的量效正相关。     

An experimental evaluation of regionally induced analgesia in dentistry.

A method for the assessment of induced regional dental analgesia in experimental animals is described. It is based on the validity of the electrodentally elicited digastric electromyogram as a pain index. This method has been applied to study the effectiveness of one local anesthetic and of "electroacupuncture" to suppress pain regionally.     

Acupuncture analgesia and its application in cranio-maxillofacial surgical procedures.

BACKGROUND: The present study investigated the use of acupuncture analgesia in maxillofacial surgery. PATIENTS AND METHODS: Acupuncture analgesia was applied in 120 patients. In 20 of these, surgery was carried out under general anaesthesia in combination with acupuncture analgesia. In 100 patients, acupuncture analgesia was applied in addition to traditional postoperative analgesia. In case of troublesome postoperative pain, it was necessary to carry out additional sessions (1-4) of acupuncture stimulation. RESULTS: When acupuncture analgesia was used, the pulse rate and blood pressure during surgery generally remained stable. In the present study serum cortisol was also measured and showed only minor elevation. DISCUSSION: The present study demonstrated favourable results when the first variant of the brake method of acupoint stimulation was used for 40-50 min with additional manual twirling of the needles. This involved inserting needles into the acupoints and, using slow, rolling manual movements increasing the amplitude step-by-step provoking simultaneous especially strong patient sensations. CONCLUSION: Acupuncture analgesia can be a useful adjunct to conventional anaesthesia in maxillofacial surgery.     

Freeway space measurement using mandibular kinesiograph and EMG before and after TENS

This investigation expands on a previous pilot study of the effects of TENS on the resting position of the mandible. The tendency is for an increase in freeway space, but the variability of results makes individual evaluation essential.     

The organization of the inferior alveolar nerve and its relation to local analgesia

An electrical stimulator was used to assess the onset of analgesia after inferior alveolar block injections. The findings were considered in relation to the anatomy of an inferior alveolar nerve, which was sectioned at three levels. It was found that lip and pulpal analgesia was achieved over a similar period, and the commonly held belief that lip anaesthesia occurs first is due to a lack of awareness of early pulpal sensory changes.     

Granulocyte colony-stimulating factor induced reduction in pulpal necrosis

Aim  To examine the effect of recombinant granulocyte colony-stimulating factor (G-CSF) on the number and function of neutrophils and on the histopathology of pulpal inflammation in normal and neutropenic rats.
Methodology  The effect of G-CSF on changes in pulpal tissue was investigated at 2, 4, 7, and 10 days after pulpal exposure of the mandibular first molar of normal rats and of those with methotrexate-induced neutropenia. The area of pulpal necrosis was measured. The neutrophil count in peripheral blood was determined, and their phagocytosis and chemotactic reaction were also examined. Statistical significance was examined by use of the two way analysis of variance.
Results  In untreated rats, G-CSF significantly ( P  < 0.05) increased the number of peripheral neutrophils and their chemotactic reaction, but did not affect pulpal inflammation. In methotrexate-induced neutropenic rats, the phagocytosis and migration of neutrophils reduced, and the area of pulpal necrosis enlarged. After the G-CSF injection, the decreases in neutrophil count and their functions significantly ( P  < 0.05) reversed, and the enlargement of pulpal necrosis inhibited.
Conclusions  These findings indicate that G-CSF prevented the reduction in neutrophil function and reduced the pulpal necrosis observed in the neutropenic rats, and suggest that neutrophils defend against bacterial invasion in pulpal tissue.