Separation of alpha-amylase isoenzymes using cellulose acetate electrophoresis

alpha-Amylase isozymes were separated by electrophoresis in cellulose acetate to detect the isoforms of the chromogenic substrate manufactured by Lachema, Czechoslovakia. In a group of 20 normal subjects aged 25 to 45, alpha-amylase pancreatic isozyme activity prevailed. Patients with acute myocardial infarction developed, during 36 hours after the onset of the anginal attack, a reduction in the ratio of pancreatic amylase/salivary amylase activities in both increased and normal total alpha-amylase activities. The suggested modified technique of electrophoretic separation of alpha-amylase isozymes may become an effective method for differential diagnosis. Use of this method will help locate the source of hyperamylasemia in various diseases and will thus specify the diagnosis, ruling out the useless therapy for pancreatitis.     

The use of o-dianisidine for serum haptoglobin electrophoresis using cellulose acetate.

1. Due to the carcinogenicity of benzidine, a method by which o-dianisidine is used to stain serum haptoglobin is described. Serum haptoglobin is determined by electrophoresis using cellulose acetate as the medium. 2. A comparison of the two staining systems demonstrates good agreement. 0-Dianisidine can be substituted for benzidine without loss of specificity.     

Apparatus for electrophoresis on cellulose acetate films]

Type EFPA-30 apparatus for electrophoresis on cellulose acetate films was developed at the Specialized Design Office for Biophysical Equipment of the Moscow Research and Production Association BIOFIZPRIBOR. Three autonomic separation cameras are united in one block, that permits separation of 12, 24, and 36 samples; the apparatus is supplied with time transducer, acoustic and visual indication. Clinical trials of the apparatus were carried out at the Institute of Biological and Medical Chemistry of the USSR Academy of Medical Sciences, its commercial production is to be started. The apparatus is intended for protein separation and may be used for studies of hemoglobins, serum proteins, lipoproteins, enzymes, and other biologic compounds. Methods for hemoglobin and serum protein separation are presented.     

Convenient immunofixation electrophoresis on cellulose acetate membrane.

Applying polyspecific antiserum onto cellulose acetate membrane after electrophoretic separation can result in a simultaneous fixation of multiple antigens as discrete bands with a resolving power of at least 30 ng protein per band.     

Haptoglobin type determination by cellulose acetate zone electrophoresis

Conditions have been presented for the determination of blood haptoglobin type by cellulose acetate zone electrophoresis. The method, which requires only 10 μl of plasma or serum, is useful for routine screening procedures.