斑秃患者外周血T淋巴细胞亚群及CD4~+CD25~+调节T细胞的检测

目的检测斑秃(AA)患者外周血T淋巴细胞亚群及CD4+CD25+调节性T(Tr)细胞数量变化,分析AA的可能病因。方法利用流式细胞仪和单克隆荧光抗体技术,测定重度和局限性AA各40例患者外周血中T淋巴细胞亚群占T淋巴细胞的比率及CD4+CD25+Tr细胞在CD3+CD4+T淋巴细胞中的比率。结果重度AA患者外周血中CD4+CD25+Tr细胞占CD3+CD4+T细胞的比率为(1.43±0.74)%,显著低于正常对照组(2.25±0.97)%(P<0.01),重度AA患者的CD4+T占T淋巴细胞的比率为(31.42±6.66)%,略高于正常对照组(30.69±7.47)%(P>0.05),差异无显著性,而CD8+T占T淋巴细胞的比率为(25.86±4.35)%,明显高于正常对照组(22.42±6.10)%(P<0.01);局限性AA患者的三项指标分别为(2.14±0.87)%,(32.60±10.27)%和(21.59±5.24)%,与对照组差异无显著性(P>0.05)。结论AA患者外周血中CD4+CD25+Tr明显低于正常对照组,CD8+T比率明显高于正常对照组,可能是导致重度AA发病的主要免疫机制。     

散发型白癜风患者外周血CD4~+/CD8~+T细胞比值及CD4~+CD25~+T细胞的检测

目的检测散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+调节性T细胞水平,探讨其与散发型白癜风发病的关系。方法散发型白癜风患者29例,男13例,女16例。通过流式细胞仪对散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+调节性T细胞水平进行检测,并与20例健康人相比较。结果与健康对照组相比,散发型白癜风患者外周血中CD4+/CD8+T细胞比值的差异无统计学意义(P0.05),而CD4+CD25+调节性T细胞水平明显减少,差异有统计学意义(P0.05),但在不同病程的患者中CD4+CD25+调节性T细胞数量的差异无统计学意义(P0.05)。结论散发型白癜风患者外周血中存在CD4+CD25+调节性T细胞水平下降,可能与散发型白癜风的发生发展有一定关系。     

银屑病和白癜风患者外周血CD4+CD25+调节性T细胞的检测

目的: 检测CD4+CD25+调节性T细胞(Treg)在白癜风和银屑病患者外周血中的表达.方法: 用流式细胞术检测19例白癜风患者、18例银屑病患者及19名正常人外周血中CD4+CD25+Treg的表达水平.结果: 白癜风患者的CD4+CD25+Treg表达率为(1.056±0.662)%,低于正常对照组(2.022±0.98)%,差异有统计学意义(P＜0.05);银屑病患者的CD4+CD25+Treg为(1.761±1.396)%,与正常人无显著性差异(P＝0.177).结论: CD4+CD25+Treg细胞在白癜风患者外周血中数量明显减少.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

目的 检测不同病期白癜风患者外周血CD4+CD25+调节性T细胞水平,探讨其与白癜风发病的关系.方法 白癜风患者34例,进展期19例,稳定期15例.通过流式细胞仪对不同病期白癜风患者外周血CD4+、CD4+CD25+T细胞水平进行检测,并与20例正常人比较.结果 进展期患者外周血中CD4+CD25+调节性T细胞占外周血淋巴细胞的表达率低于正常对照组(P<0.05);稳定期患者与正常对照组比,差异无统计学意义(P＞0.05);进展期患者低于稳定期患者,差异有统计学意义(P<0.05).进展期患者CD4+CD25+调节性T细胞占外周血淋巴细胞表达率与皮损面积呈负相关(P<0.05),稳定期则无相关性(P＞0.05).进展期与稳定期患者CD4+CD25+调节性T细胞占外周血淋巴细胞水平与病程均无明显相关性(P＞0.05).结论 白癜风患者外周血中存在异常比例的cD4+CD25+调节性T细胞,可能与白癜风的发病有关.     

寻常型银屑病患者外周血CD4~+CD25~+及CD8~+CD25~+细胞的检测

目的研究进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞的数量变化及其在银屑病免疫病理学发病机制中的作用。方法应用流式细胞术对进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞进行检测。结果进展期寻常型银屑病外周血CD4+CD25+细胞及CD8+CD25+调节性T细胞数量与正常对照组相比,均显著降低(P<0.05,P<0.005),而CD4+CD25+/CD8+CD25+比值无显著性差异(P>0.05)。结论寻常型银屑病的发病与CD4+CD25+和CD8+CD25+调节性T细胞的同步降低有关,与二者的比值无关。     

斑秃患者皮损中CD4+和CD8+T细胞的检测

斑秃病因未明．细胞免疫可能参与了斑秃的发病．CD4^ 和CD8^ T细胞是两种重要的免疫细胞，在免疫调节中起重要作用。为了研究这两种细胞在斑秃发病中的作用．笔者采用免疫组化的方法对斑秃应损和正常头皮中CD4^ 和CD8^ T细胞的分布情况进行了检测．现报告如下。     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者表皮内郎格罕细胞观察

用改良的Ｊｕｈｌｉｎ－ＳｈｅｌｌｅｙＡＴＰ酶细胞化学染色法检查了１３例白癜风（进展期）患者的白斑、白斑边缘区及对侧相应正常皮肤中郎格罕细胞情况，结果白斑部位表皮郎格罕细胞数目较对应正常皮肤表皮变化不大，而白斑边缘部皮肤郎格罕细胞数目较正常皮肤显著增多（Ｐ＜０．００５）。在白斑及白斑边缘区还可见到印格罕细胞的形态学变化：胞体变大、深染、胞突消失以及细胞积聚现象，在白斑边缘部尤其明显。本研究显示郎格罕细胞数目和形态学的变化与病情活动有关。提示郎格罕细胞在白癜风发病机制中起一定的作用。     

白癜风患者外周血CD_4,CD_8检测及免疫调节剂对其影响

目的观察白癜风患者外周血CD4,CD8细胞的变化及免疫调节剂在白癜风综合治疗中的作用。方法采用流式细胞术分别对符合条件的局限型白癜风患者及泛发型白癜风患者各20例进行外周血CD4,CD8测定,并与正常对照组比较;对泛发型白癜风患者给予免疫调节剂等综合治疗。结果局限型白癜风患者CD4,CD8基本正常,泛发型白癜风患者CD4下降,CD8升高,使用免疫调节剂治疗后CD4细胞有不同程度升高,CD8细胞下降。结论泛发型白癜风患者多数存在细胞免疫功能低下,使用免疫调节剂治疗可以有效提高患者的细胞免疫功能。     

银屑病患者体外系统血液固有免疫反应中CD4~+ CD25~+ T细胞及CD4~+ CD25~(high) T细胞的变化与意义

目的用体外系统血液固有免疫反应体系研究银屑病患者外周血CD4+ CD25+T细胞及CD4+ CD25highT细胞的变化,并探讨其在银屑病发病机制中的作用。方法将灭活大肠杆菌悬液(3×108/mL)0.2mL作为免疫原激活剂加入枸橼酸抗凝的全血细胞悬液0.2mL和血浆0.3mL中,37℃水浴1h,用流式细胞仪测定CD4+ CD25+T细胞及CD4+ CD25highT细胞的比例。结果在加入大肠杆菌的银屑病全血细胞组(银屑病实验组)CD4+ CD25highT细胞比例(1.88%)明显高于银屑病对照组(1.41%)(P﹤0.01),CD4+ CD25+T细胞及CD4+ CD25highT细胞的比例(16.86%,1.88%)明显低于加入大肠杆菌的健康实验组(24.26%,2.81%)(P﹤0.01);银屑病对照组CD4+ CD25+T细胞及CD4+ CD25highT细胞的比例(15.97%,1.41%)较健康对照组明显降低(21.75%,2.17%)(P﹤0.01);健康实验组CD4+ CD25+T细胞及CD4+ CD25highT细胞的比例群(24.26%,2.81%)均明显高于健康对照组(21.75%,2.17%),(P﹤0.05)。结论银屑病患者CD4+ CD25+T细胞及CD4+ CD25highT细胞比例降低,外来抗原刺激后比例升高,但与健康正常人存在差异。这可能与其复杂的系统免疫学发病机制相关。     

梅毒患者外周血CD4~+、CD8~+调节性T细胞表达的临床意义

目的:监测梅毒患者外周血CD4~+、CD8~+调节性T细胞的表达水平,考察其在临床的参考价值。方法:将2014年12月至2015年9月,我院收治并确诊的32例梅毒患者作为研究对象。其中,Ⅰ期、Ⅱ期、潜伏期梅毒患者分别有11例、15例和6例。采用细胞免疫芯片检测(淋巴细胞CD4~+、CD8~+绝对数检测)技术检测并计算患者外周血中的调节性T细胞CD4~+、CD8~+表达水平及CD4~+、CD8~+的比值。并与同期就诊我科的30例非梅毒患者的相应指标数据进行比较。结果:与非梅毒患者比较,梅毒患者外周血中CD4~+、CD8~+细胞绝对值计数均显著下降(P0.05);CD4~+、CD8~+比值显著低于非梅毒患者组(P0.05)。在Ⅰ期、Ⅱ期、潜伏期梅毒患者间CD4~+、CD8~+绝对值计数均值水平的差异有统计学意义(P0.05)。结论:在梅毒患者外周血中CD4~+、CD8~+表达水平及CD4~+、CD8~+比值均显著降低,而在梅毒感染的不同疾病发展时期CD4~+、CD8~+表达水平也有着显著的差异。     

系统性红斑狼疮患者CD4~+ CD25~+调节性T细胞及其细胞因子的改变

目的探讨系统性红斑狼疮(SLE)患者CD4+CD25+调节性T细胞(Tr)及其细胞因子的改变。方法用流式细胞仪直接免疫荧光法检测SLE患者外周血CD4+CD25+T细胞的百分率,FoxP3+分子表达,细胞因子IL-10,TGF-β1的表达。结果 SLE患者活动期组、稳定期组、健康对照组CD4+CD25+T细胞占CD4+T细胞的百分率分别是(3.57±1.45)%,(5.14±1.63)%,(6.03±1.96)%。活动期患者明显低于稳定期患者和健康对照组,差异均有统计学意义(P均<0.05)。CD4+CD25+FoxP3+T细胞、CD4+CD25-FoxP3+T细胞占CD4+T细胞的百分率,活动期患者明显高于稳定期患者和健康对照组,差异均有统计学意义(P<0.05)。CD4+CD25+FoxP3+T细胞与疾病的活动指数(DAI)正相关(r=0.659,P<0.05);而CD4+CD25-FoxP3+T细胞与DAI无统计学相关性(r=0.184,P>0.05)。活动期患者CD4+CD25+IL-10+细胞明显高于稳定期患者和健康对照组,差异均有统计学意义(P均<0.05)。然而CD4+CD25-TGF-β+细胞在活动期患者、稳定期患者和健康对照组之间比较差异无统计学意义(P均>0.05)。结论 SLE患者外周血中Tr细胞数量减少,分泌的IL-10增加,TGF-β减少,可能导致细胞免疫抑制功能减弱,自身免疫耐受平衡被打破,出现激活的自身反应性T细胞增多;此外,T淋巴细胞激活增多,还可辅助B细胞产生更多相关抗体,表现出多种临床症状。     

HIV/AIDS患者CD4~+ T细胞中CD25和CD8~+ T细胞中CD28的表达

目的探讨外周血CD4+ T细胞中CD25表达率和CD8+ T细胞中CD28表达率在HIV/AIDS患者发病中的作用。方法应用流式细胞仪荧光染色技术检测35例HIV/AIDS患者CD4+ T细胞中CD25表达和CD8+ T细胞中CD28的表达,以41名健康体检人员做对照。结果HIV/AIDS患者和健康对照组之间CD4+ T细胞中CD25表达率(27.51±4.23)%,(44.41±9.17)%,CD4+25+ T细胞占淋巴细胞的比例(2.00±1.42)%,(16.62±4.60)%,CD4+25- T细胞占淋巴细胞的比例(5.16±3.37)%,(21.03±6.19)%,CD8+ T细胞中CD28中的表达率(25.12±6.33)%,(44.24±8.61)%,CD8+28- T细胞占淋巴细胞的比例(36.85±8.98)%,(13.33±4.58)%的差异均有显著性(P<0.01),CD8+ 28+ T细胞占淋巴细胞的比例(12.31±4.14)%,(10.51±3.71)%差异无显著性(P>0.05)。结论HIV/AIDS患者CD25在CD4+ T细胞与CD28在CD8+ T细胞的表达率降低可能与HIV感染后引起的免疫缺陷有关,CD8+ CD28- T细胞的增加有助于促进HIV/AIDS患者的炎性反应和免疫激活。     

系统性硬皮病CD4+T细胞中CD70mRNA及蛋白表达水平的观察

目的:研究系统性硬皮病(SSc)患者外周血CD4+T细胞中CD70的表达水平.方法:应用密度梯度离心法分离17例SSc患者(女性12例,男性5例)和13例对照者(女性8例,男性5例)的外周血单个核细胞,磁珠分选CD4+T细胞.RT-PCR检测CD4+T细胞中CD70 mRNA的表达水平;流式细胞术检测CD70蛋白在CD4+T细胞的表达水平.结果:与对照组相比,SSc患者CD4+T细胞中CD70 mRNA和CD70蛋白表达均明显升高(P=0.001;P=0.007).结论:CD70在SSc的发生发展中可能起着重要作用.