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����գ� κ������ �ƺ�ΰ�� �������� ������ ������ ���»� 《中国药学杂志》2016,51(20):1803-1806
??OBJECTIVE To discuss the role of proficiency testing program for pharmaceutical dissolution determination in the capacity building of relevant laboratories. METHODS Based on the results of the two proficiency testing programs carried out in recent years, analyze the difference of the quality management and the level of dissolution determination among the participating laboratories and discuss on the way that the proficiency testing programs carried out in future. RESULTS There is difference among the laboratories in both the quality management and the level of dissolution determination, the "unsatisfactory" laboratories mainly concentrated in local drug control institutes and pharmaceutical manufacturers. The three "unsatisfactory"laboratories in the first program, took corrective and preventive measures, participated in the later program, and achieved "satisfactory" results. CONCLUSION The proficiency testing program is effective in improving the dissolution determination capacity of the participating laboratories and is recommended to be conducted continually in the pharmaceutical industry. 相似文献
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??OBJECTIVE To investigate the protective effect of epalrestat on right ventricular remodeling in a rat model of monocrotaline-induced pulmonary arterial hypertension(PAH). METHODS PAH rats were induced by a single injection of monocrotaline(60 mg??kg-1, sc) and were administered epalrestat(50 or 100 mg??kg-1) for 4 weeks. At the end of experiment, the right ventricular systolic pressure(RVSP) and mean pulmonary artery pressure(mPAP) were monitored via the right jugular vein catheterization into the right ventricle. Right ventricle(RV) and left ventricle(LV)+septum(S) were isolated and weighed, and ratios of RV/(LV+S)and RV to tibial length were calculated. Right ventricular morphological change was observed by HE staining. Masson??s trichrome stain was used to demonstrate collagen deposition. The total antioxidative capacity(T-AOC) and malondialdehyde(MDA) levels in right ventricle were determined according to the manufacturer??s instructions. The expression of collagen I, collagen ??, AR and NOX4 were analyzed by immunohistochemisty, real-time PCR or Western blot. RESULTS The results showed that epalrestat treatment for 4 weeks attenuated RVSP, mPAP and right ventricular remodeling index(RV/LV+S and RV/Tibial length) of PAH rats induced by monocrotaline. Furthermore, monocrotaline-induced right ventricular collagen accumulation and collagen I and collagen III expression were both significantly suppressed by epalrestat. The expressions of AR, NOX4 and MDA content were obviously decreased, while the T-AOC was significantly increased in right ventricular from PAH rats with epalrestat treatment. CONCLUSION These results suggest that epalrestat ameliorates right ventricular remodeling of PAH induced by monocrotaline in rats through its down-regulating of AR and NOX4 expression and collagen accumulation. 相似文献
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??OBJECTIVE To investigate the cardioprotective effect of exogenous zinc (Zn2+)on the mitochondrial pathway, and to explore its possible mechanism. METHODS Rat heart tissue-derived H9c2 cardiac cells were cultured, and then randomly divided into control group, ZnCl2 group (1-20 ??mol??L-1, 20 min), ZnCl2 plus inhibitor group [PI3K inhibitor LY294002, 10 ??mol??L-1 and mitochondrial ATP sensitive potassium channel (mKATP) inhibitor 5-HD, 0.5 mmol??L-1, inhibitors treated cells for 10 min and then ZnCl2 20 min] and inhibitor group (10 min). The mitochondrial permeability transition pore (mPTP) opening was evaluated by measuring mitochondrial membrane potential (????m). Tetramethylrhodamine ethyl ester (TMRE) diacetate fluorescence images were obtained with laser scanning confocal microscopy. GSK-3?? and AKT phosphorylation were determined with Western blot. The cells were subjected to simulated ischemia/reperfusion injury, cell viability were determined with flow cytometry. Cells were transfected with constitutively active GSK-3??-S9A(GSK-3??-S9A)plasmid by Fugene 6 transfection kit, mPTP opening was evaluated by confocal microscopy. RESULTS Compared with the normal, exposure of cells to H2O2 for 20 min caused a marked decrease in TMRE fluorescence, treatment of cells with different dose of Zn2+ prevented the loss of TMRE fluorescence caused by H2O2 with the peak at 10 ??mol??L-1. Western blot showed that Zn2+significantly enhanced the GSK-3?? and AKT phosphorylation, the effect that was significantly reversed by LY294002, but not 5-HD. Compared with the normal, ischemia/reperfusion markedly reduced cell viability. Zn2+ applied at ischemia did not increase the cell viability, but significantly increased the cell viability when given at reperfusion. Zn2+could mimic the specific mPTP inhibitor cyclosporin A (1 ??mol??L-1) and prevent the mPTP opening, which was again reversed by LY294002 but not 5-HD. Zn2+ was not able to exert protection in cells transfected with the GSK-3??-S9A. CONCLUSION Zn2+ can induce myocardial mitochondrial protective effect by modulating the mPTP opening through the inactivation of GSK-3?? via PI3K/AKT pathway. mKATP may not be involved in the action of Zn2+. 相似文献
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??OBJECTIVE To investigate the apoptosis of the auction and mechanism of the hepatoma BEL-7402 cells induced by the ginseng polysaccharides(GPS). METHODS The hematoma cells BEL-7402 were incubated with GPS, cell viability was measured by CCK8, cell cycle distribution was assessed by fluorescence-activated cell sorting(FACS), the cell morphological changes were traced with TUNEL and scanning electron microscope, TNFR1, BCL-2 and Bax protein expression and ERK phosphorylation are tested by Western blot. RESULTS CCK8 results showed that GPS inhibited cells growth of BEL-7402 in dose-dependent and time-dependent. Flow cytometry found that S phase arrest was increased upon GPS concentrations. Obviously apoptotic sub-g peak was also found. And the peak was gradually enhanced with the concentration increased. TUNEL staining and SEM results showed that GPS led to significant cell morphology changes in hepatoma cells BEL-7402.And the apoptosis effect was increased upon the GPS concentrations. Western blot showed that level of apoptosis related protein Bax was increased, the expression of apoptosis-antagonizing protein Bcl-2 was decreased and the expression of death receptor TNFR1 appeared with GPS concentrations increased gradually,raise ERK phosphorylation. CONCLUSION GPS can induce apoptosis of hepatoma cells BEL-7402 by the mitochondrial pathway and the death receptor dependent pathway and ERK pathway. 相似文献
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??The rapid development of modern analytical technology provides many methods of detecting drugs and their metabolites. LC-MS/MS technology has become one of the most commonly used methods in drug metabolites analysis with its characteristics of simpleness and high efficiency. In this paper, based on the published articles of in vivo drug metabolites study by LC-MS/MS in recent years, the processes of data acquisition and processing, metabolites detecting and identification and software-assisting were summarized. This article provides references for research on drug metabolism using LC-MS/MS. 相似文献
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??OBJECTIVE To examine the expression of 15-hydroxyprostaglandin dehydrogenase(15-PGDH) in human multidrug-resistant breast cancer line MCF-7/ADR and to explore the reversal effect and mechanism of 15-PGHD induction drugs on MCF-7/ADR cells. METHODS The RT-PCR and Western blot were used to detect 15-PGDH, COX-2 mRNA and protein expression in MCF-7 and MCF-7/ADR cells. PGE2 levels in supernatant of cells were determined by ELISA assay. Anti-proliferation effect and chemotherapy sensitivity to ADM of 15-PGDH induction drugs (indomethacin, ibuprofen and pioglitazone, dexamethasone) on breast cancer cells were assayed by MTT method. Cell apoptosis was detected by Hochest 33258 stain assay. RESULTS Compared with MCF-7 cells, the 15-PGDH expression was significantly decreased, COX-2 expression was significantly increased and PGE2 levels in cell supernatant were increased in MCF-7/ADR cells. 15-PGDH induction drugs (indomethacin, ibuprofen and pioglitazone, dexamethasone) increased 15-PGDH expression or both reduced COX-2 expression, and finally reduced PGE2 levels in MCF-7/ADR cells. Effect of chemosensitivity and apoptosis induction of ADM was enhanced and multidrug resistance was partially reversed when co-treated with 15-PGDH induction drugs. CONCLUSION The expression of 15-PGDH is decreased in human multidrug-resistant breast cancer line MCF-7/ADR. 15-PGDH induction drugs could increase chemosensitivity, promote apoptosis and reverse resistance of MCF-7/ADR cell, the mechanism might related to the influence of PGE2 level by regulated the expression of 15-PGDH and COX-2. 相似文献
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??OBJECTIVE To investigate the therapeutic effects metabolic mechanism of Schisandra chinensis polysaccharide (SCP) on chronic fatigue syndrome (CFS). METHODS CFS rat model was established in a variety of ways such as the bondage, excessive exercise, crowded and noise environment. The Morris water maze test, the open-field test and the tail-suspension test were performed to evaluate the CFS model. The gas chromatography-mass spectrometry (GC-MS) method was conducted to screen the different metabolites in rat urine and analyze the metabolic pathway. RESULTS The body weight of rats were increased and their space exploration and memory ability were strengthened after SCP supplement. The eleven diversity urine metabolites were detected and the involved metabolic pathways were the tricarboxylic acid cycle and the alanine, aspartic and glutamic acid metabolic pathways. CONCLUSION SCP could relieve the chronic fatigue syndrome. The metabolic mechanism is relative to the improvement of SCP on the tricarboxylic acid cycle and the alanine, aspartic and glutamic acid metabolic pathways. 相似文献
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??OBJECTIVE To establish an improved reversed-phase high performance liquid chromatography method coupled with pulsed electrochemical detection for determining the related substances of netilmicin sulfate injection. METHODS Agilent Proshell 120 SB-C18 column (4.6 mm×150 mm,2.7 μm)and gradient elution were used. Mobile phase A was 0.2 mol·L-1 trifluoroacetic acid in 0.1 mol·L-1 sodium hydroxide solution-acetonitrile (97:3), mobile phase B was 0.1% pentafluoropropionic acid-acetonitrile (97:3), and the flow rate was 0.8 mL·min-1. A pulsed electrochemical detector was adopted, and the temperatures of detector and column were kept at 35 ??. The working electrode was a gold electrode with diameter of 3 mm and a quadruple-potential waveform (QPW)was selected as detection waveform. The injection volume was 25 μL. NaOH solution of 0.8 mol·L-1 was added post-column at a flow rate of 0.3 mL·min-1. RESULTS A total of 28 impurities could be detected and effective separation was achieved in the typical sample and most of which could not be separated in the method of Ch.P 2015. The linearity of the calibration curve for netilmicin ranged from 0.25 to 15 μg·mL-1 with a coefficient of determination equal to 0.999 1. The LOD and LOQ of netilmicin were found to be 0.25 ng and 1.25 ng, respectively. The repeatability RSD(n=6) of the single largest impurity and total impurities were 0.9% and 0.8%, respectively. The sample solution was stable within 24 h. CONCLUSION Compared with previously published investigations, the improved method shows higher sensitivity, better separation ability and good reproducibility, especially for differentiating the origin of bulk drug for netilmicin sulfate injection, thus is more suitable for the determination of related substances of netilmicin sulfate injection. 相似文献
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?? Zingiber officinale has a long application history in China, it is used as medicine, also condiment, food and drinks. The chemical constituents of Zingiber officinale include volatile oil, gingerol,diaryl-heptnaoids and so on. Scientific research showed that Zingiber officinale is widely used in anti-nausea, resisting gastric ulcer, anti-bacterial, anti-phlogistic, analgesia, antioxidant, resisting motion sickness, anti-tumor, hypoglycemic,antilipidemic,and improving cardiocerebral vascular system and so on.Zingiber officinale is recommended as a healthy food by doctor of traditional Chinese medicine in all times. In summary, it's worth to be researched and developed in detail. 相似文献
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??OBJECTIVE To explore the effects of luteolin on the proliferation and migration of the endothelial cells co-cultured with cancer cells and its molecular mechanism. METHODS Human umbilical vein endothelial cells (HUVECs) were primarily isolated and identified by the expression of VE-cadherin. Cancer-endothelial cell co-culture model was established using the Transwell system. The effects of luteolin at different concentrations (0 [Co-culture control], 20 and 50 ??mol??L-1) on the proliferation and migration of HUVECs in the co-culture system were determined. A HUVECs control group removed of prostate cancer PC3 cells was also included. Human angiogenesis antibody array kit was used to assay the secretion levels of various protein factors in each group. RESULTS VE-cadherin was expressed on all the cultured HUVECs. Increased proliferation ability was found in the HUVECs co-cultured with PC3 cells compared with that in HUVECs control group (P<0.01). Treatment with 20 or 50 ??mol??L-1 luteolin significantly inhibited the proliferation ability of the HUVECs in co-culture system (both P<0.01). Increased migration ability was found in the HUVECs co-cultured with PC3 cells compared with that in HUVECs control group (P<0.01). Treatment with 20 or 50 ??mol??L-1 luteolin significantly inhibited the migration ability of the HUVECs in co-culture system (both P<0.01). Secretion levels of multiple angiogenesis-related proteins in the cultural supernatant of co-culture system were significantly increased compared with those in HUVECs control group. Treatment with 20 ??mol??L-1 luteolin significantly inhibited the secretion levels of interleukin-8 (IL-8), vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1(MCP-1) in the cultural supernatant of co-culture system (all P<0.01). CONCLUSION Luteolin may inhibit the proliferation and migration of endothelial cells via suppressing the secretions of IL-8, VEGF and MCP-1 in cancer-endothelial co-culture system. 相似文献
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目的:研究白藜芦醇苷(polydatin)在体外对乳腺癌细胞MCF-7增殖的作用,进一步探索其潜在的机制。方法:设定空白组及白藜芦醇苷(0.2,0.4,1.2,2.0,2.8 mg·L~(-1))组,5-氟尿嘧啶组,采用噻唑蓝(MTT)比色法测定不同质量浓度白藜芦醇苷对MCF-7细胞的生长抑制率;应用细胞克隆集落形成实验验证白藜芦醇苷对MCF-7细胞增殖的抑制作用;通过实时荧光定量PCR(Real-time PCR)检测5-氟尿嘧啶(1.0μg·L~(-1))和白藜芦醇苷(0.2,0.4,1.2 mg·L~(-1))对DNA聚合酶δ催化亚基P125(P125),P125编码基因(POLD1),p53基因(p53),细胞周期依赖性蛋白激酶抑制因子p21 mRNA表达水平的影响;应用蛋白质免疫印迹法(Western blot)检测白藜芦醇苷(0.2,0.4,1.2 mg·L~(-1))对P125,p53,p21蛋白表达水平的影响。结果:与空白组比较,白藜芦醇苷(0.2,0.4,1.2,2.0,2.8 mg·L~(-1))在体外对MCF-7细胞具有明显的抑制作用(P0.05,P0.01),并且这种抑制作用呈浓度依赖性。与空白组比较,白藜芦醇苷(0.2,0.4,1.2 mg·L~(-1))均能明显抑制细胞集落的形成(P0.05,P0.01)。与空白组比较,5-氟尿嘧啶组和白藜芦醇苷(0.4,1.2 mg·L~(-1))能够显著抑制POLD1 mRNA表达水平,同时显著抑制P125 mRNA和蛋白表达水平,明显升高p53,p21 mRNA和蛋白表达水平(P0.05,P0.01)。与5-氟尿嘧啶组比较,白藜芦醇苷(1.2 mg·L~(-1))对POLD1,P125,p53,p21 mRNA和蛋白表达水平影响均无显著差异。结论:白藜芦醇苷在体外能够显著抑制MCF-7细胞的增殖,其潜在机制可能与提高p53表达水平,抑制POLD1表达,促进p21表达有关。 相似文献
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目的:观察人参多糖注射液对人肝癌细胞BEL-7402和HpeG2增殖抑制作用,并探讨其机制。方法:将不同浓度的人参多糖注射液与人肝癌细胞BEL-7402和HpeG2作用,采用cell counting kit-8(CCK8)检测细胞的增殖抑制,通过倒置显微镜观察用药前后细胞形态、数量变化,透射电镜观察细胞坏死、凋亡等形态学变化,蛋白免疫印迹法(Western blot)检测肿瘤坏死因子受体1(TNFR1)蛋白表达情况。结果:人参多糖注射液对人肝癌细胞BEL-7402和HpeG2的增殖有明显抑制作用(P0.05),并且与浓度和时间呈正相关。60,80,100 g·L~(-1)人参多糖注射液作用于人肝癌细胞BEL-7402和HpeG2 24,48,72h后,可显著抑制其增殖(P0.01),40 g·L~(-1)人参多糖注射液作用于人肝癌细胞BEL-7402和HpeG2 24,48,72 h后可明显抑制其增殖(P0.05)。人参多糖注射液作用人肝癌细胞BEL-7402和HpeG2 48 h后,细胞出现细胞核边集、凋亡小体等凋亡形态变化,72 h后部分细胞出现空泡样变等形态学变化。与空白组比较,40,60,80 g·L-1人参多糖注射液组作用人肝癌细胞BEL-7402和HpeG2后,TNFR1蛋白相对表达量明显升高(P0.05,P0.01)。结论:人参多糖注射液对人肝癌细胞BEL-7402和HpeG2的增殖有明显的抑制作用;诱导细胞凋亡可能是抑制作用的机制。 相似文献
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目的 探讨不同浓度葛根素对大鼠肾小球系膜细胞增殖的影响及其作用机制。方法 采用体外细胞培养技术,采用MTT检测不同浓度葛根素对大鼠肾小球系膜细胞增殖的量效和时效关系。分别采用RT-PCR和ELISA,检测不同浓度葛根素作用大鼠肾小球系膜细胞后,结缔组织生长因子mRNA和蛋白表达水平的变化。结果 葛根素可抑制大鼠GMCs增殖(P<0.05),该增殖抑制作用呈浓度依赖模式。不同浓度葛根素作用于大鼠GMCs后,结缔组织生长因子mRNA和蛋白表达水平均随药物浓度增加而逐渐减弱(P<0.05)。结论 葛根素对大鼠肾小球系膜细胞增殖有明显的抑制作用,其作用机制可能与下调结缔组织生长因子基因转录和表达水平有关。 相似文献
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目的探讨戊地昔布对小鼠肠癌clone 26细胞的生长抑制作用及机制。方法用噻唑蓝(MTT)法检测戊地昔布对clone 26细胞生长的作用。用流氏细胞仪检测clone 26细胞凋亡率和细胞周期分布。用Western blot检测clone 26细胞caspase-3,PCNA和COX-2的表达。结果①戊地昔布可抑制clone 26细胞生长并呈时间和浓度依赖性。②50~400μmol·L-1戊地昔布可明显提高clone 26细胞的凋亡率,从对照的3.1%提高到4.4%~11.0%。给予戊地昔布后,细胞的增殖指数,S期的细胞比例和G2/M期细胞有下降趋势,但只有在400μmol·L-1戊地昔布组时才有统计学意义。③给予戊地昔布后,细胞PCNA的表达降低,caspase-3的表达升高,COX-2的表达没有变化。结论戊地昔布通过诱导凋亡和细胞周期停滞而抑制clone 26细胞生长,小剂量时主要与凋亡有关,大剂量时与诱导凋亡和细胞周期停滞有关。Caspase-3参与戊地昔布诱导的凋亡。 相似文献
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独角莲含药血清对肿瘤细胞的增殖抑制和诱导凋亡作用的研究 总被引:2,自引:0,他引:2
目的:观察独角莲含药血清在体外对K562白血病细胞增殖及凋亡的影响。方法:采用血清药理学方法制备独角莲含药血清,以不同浓度的含药血清处理体外培养的K562白血病细胞,采用MTT比色法观察独角莲含药血清对K562细胞增殖的影响;采用流式细胞仪(FCM)及DNA片段凝胶电泳观察和检测细胞凋亡情况。结果:不同浓度独角莲含药血清对K562细胞增殖具有抑制作用,并呈剂量依赖关系。当含药血清作用96h后,其抑制作用开始减弱。流式细胞仪检测显示,独角莲含药血清能够诱导K562细胞的凋亡,且凋亡率随着剂量的增加而增大,与空白对照组比较(P〈0.01);DNA琼脂糖凝胶电泳谱可见典型的DNA梯形带形成。结论:独角莲含药血清具有抑制K562白血病细胞增殖、促进细胞分化和诱导细胞凋亡的作用,其作用强度与时间-浓度呈正相关。独角莲含药血清对K562细胞发生细胞毒作用可能与诱导K562细胞凋亡有关。 相似文献
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目的:探讨牛蒡子苷元(ARG)对子宫内膜癌HEC-1B细胞的增殖抑制作用及机制。方法:HEC-1B细胞分为观察组和对照组,观察组用终浓度为10、20、30、40、60、100μmol/L的ARG干预,对照组用0.5%二甲基亚砜(DMSO)干预。48 h后,用四甲基偶氮唑盐微量酶反应比色法(MTT法)检测胞增殖情况;流式细胞仪检测细胞凋亡及细胞周期;蛋白免疫印迹法(WB)检测细胞NF-κB信号通路相关蛋白表达。结果:10、20、30、40、60、100μmol/L ARG干预48 h,HEC-1B细胞的相对生存率分别为(92.36±0.52)%、(89.59±0.74)%、(78.49±0.68)%、(56.47±0.59)%、(40.12±0.69)%、(37.52±0.58)%,且随着ARG作用浓度的升高,HEC-1B细胞的相对生存率呈逐渐降低趋势,差异有统计学意义(P0.05),干预48 h HEC-1B细胞50%抑制浓度(IC50)为50μmol/L。对照组和观察组(50μmol/L ARG)细胞凋亡率分别为(2.89±0.56)%、(26.58±3.26)%,观察组细胞凋亡率明显高于对照组,差异有统计学意义(P0.05)。但观察组细胞周期分布与对照组比较,差异无统计学意义(P0.05)。观察组(50μmol/L ARG)细胞p65、p-IκB-α蛋白表达量明显低于对照组,差异有统计学意义(P0.05),2组间IκB-α蛋白表达量差异无统计学意义(P0.05)。结论:ARG可抑制HEC-1B细胞增殖,促进其凋亡,其作用机制与可能与降低NF-κB通路相关蛋白表达水平,抑制NF-κB通路活化有关。 相似文献
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目的本研究旨在研究去氢骆驼蓬碱体外诱导Jurkat细胞凋亡及其可能机制。方法采用MTT法测定去氢骆驼蓬碱对Jurkat细胞的生长抑制作用;用光学显微镜检、琼脂糖凝胶电泳检测去氢骆驼蓬碱诱导Jurkat细胞凋亡;应用蛋白印迹法检测去氢骆驼蓬碱诱导Jurkat细胞凋亡过程中bcl-2与ICAD的表达变化情况。结果去氢骆驼蓬碱对Jurkat细胞有明显的生长抑制作用,其增殖抑制作用与诱导细胞凋亡有关;bcl-2与ICAD在Jurkat细胞中均有表达,且随药物浓度的增高表达量逐渐降低。结论去氢骆驼蓬碱可诱导Jurkat细胞凋亡,bcl-2与ICAD在去氢骆驼蓬碱诱导Jurkat细胞凋亡的信号传导途径中可能是重要的调控因子。 相似文献
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目的:研究青蒿抗热应激作用及其机理。方法:利用高温高湿环境造成家兔热应激模型,观察青蒿总香豆素对家兔肛温上升及血液、肺组织磷脂酶A2活性和循环内皮细胞数目的变化,并测定其对脑、心脏、肾组织钠泵活性及小鼠抗脑缺氧能力的影响。结果:青蒿总香豆素可明显降低热应激家兔体温上升速度,并伴随血清、肺组织磷脂酶A2活性降低,循环内皮细胞减少,显著抑制脑、心、肾组织钠泵活性,延长断头小鼠张口喘气时间几近1倍。结论:香豆素部位可能是青蒿祛暑功效的活性成分群之一。 相似文献
20.
目的:探究黄芩苷对人前列腺癌细胞(PC3)细胞增殖和侵袭能力的影响及其机制。方法:通过CCK-8检测黄芩苷对PC3细胞增殖的作用,Transwell实验检测黄芩苷对PC3细胞侵袭能力的作用,Western blot检测黄芩苷处理后PC3细胞E-钙黏蛋白和促凋亡基因bax,caspase-3,抗凋亡基因bcl-xl以及自噬相关基因beclin-1和LC3B-Ⅱ的表达。结果:黄芩苷抑制PC3细胞的增殖和侵袭能力,且呈浓度依赖趋势;黄芩苷能促进PC3细胞E-钙黏蛋白表达;黄芩苷能上调PC细胞促凋亡基因bax和caspase-3的表达,抑制抗凋亡基因bcl-xl表达。黄芩苷能促进PC3细胞中自噬相关基因beclin-1和LC3B-Ⅱ的表达。结论:黄芩苷可以通过促进凋亡和自噬发生,增加E-钙黏蛋白的表达,抑制PC3细胞的增殖和侵袭能力。 相似文献