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??OBJECTIVE To discuss the effect and mechanism of supplementing qi and nourishing yin(SQNY) decoction on intestinal microbiome of mice with Lewis lung carcinoma after treated with cisplatin. METHODS The model of C57BL/6 mice with lung carcinoma xenograft was established by subcutaneous seeding of Lewis cells and randomly divided into 4 groups (n=16), including saline group (NC group), cisplatin group (DDP group), CAP group (CAP group) and SQNY decoction combined with cisplatin group (DDP+SQNY group). The tumor sizes, weights and intestinal microbiota were detected. The serum levels of IL-6, IL-10, and TNF-?? were detected by ELISA. The dendritic cell (DC) and macrophage subtype in spleen of mice models were detected by flow cytometry. RESULTS ??The mean tumor sizes of mice in DDP group, CAP group, DDP+SQNY groups were all smaller than NC group (P<0.05). And the mean tumor size and weight in DDP+SQNY group were less than the other three groups (P<0.05). The mean animal weights in NC or DDP+SQNY group were higher than those in DDP group and CAP group (P<0.05). ??The mean levels of serum IL-6, IL-10 and TNF-?? in DDP+SQNY group were less than those in the other three groups (P<0.05). ??The mean microbiota richness in DDP+SQNY group were less than those in the other three groups (P<0.05). The proportion of fecal microbiota was significantly changed in DDP+SQNY group, including upregulation of Firmicutes phylum and Bifidobacterium Genus, and downregulation of Bacteroidetes phylum(P<0.05). ??The amount of DC and M1/M2 macrophage subtype in spleen of DDP+SQNY group mice models were more than those in the other three groups (P<0.05). CONCLUSION It??s suggested that SQNY decoction effectively enhances the anticancer effect of cisplatin by increasing the immune function and changing the intestinal microbiota.     

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??OBJECTIVE To design and synthesize a series of hybrids of anilinopyrimidines and cinnamic acids and to find powerful anti-non-small cell lung cancer(NSCLC) drug. METHODS Compounds 3a-3k were synthesized by combining anilinopyrimidines scaffolds and cinnamic acid derivatives through amide bonds, then the anti-NSCLS activity of these compounds was studied by MTT. RESULTS Their structures were confirmed by MS and ¹H-NMR. Most of target compounds displayed higher anti-proliferative activity on EGFR-mutant H1975 cells(IC₅₀=0.83-3.31 ??molL^-1) than gefitinib(IC₅₀=8.59 ??molL^-1). CONCLUSION Compound 3b has the best inhibitory effect on H1975 cells. Therefore, 3b may be a potential anti-NSCLC agent for further investigation.     

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??OBJECTIVE To investigate the effects of nitronyl nitroxide(HPN)on the myocardial hypoxia- and the expression of apoptosis-associated proteins in hypobaric hypoxia condition mice. METHODS Sixty BALB/c mice were divided into normal control group,hypoxia model group,acetazolamide group and HPN group randomly. After single intraperitoneal injection of HPN for 30 min,the mice were exposed to a simulated high altitude of 8 000 m for 12 h. After hypoxic exposure,mice were sacrificed and the content of lactate(LD) and lactate dehydrogenase(LDH) activity in heart were determined. HIF-1,VEGF,caspase-3,Bax and Bcl-2 were detected by immunohistochemistry. RESULTS Compared with normal control group,the LD level and LDH activity in hypoxia model group increased significantly. In addition,the expression of HIF-1??,VEGF,caspase-3 and Bax were increased,Bcl-2 and Bc1-2/Bax ratio was decreased. Pre-treated with HNP the LD level and LDH activity,the expression of HIF-1??,VEGF,caspase-3 and Bax were decreased effectively,the expression of Bcl-2 and Bc1-2/Bax ratio was increased. CONCLUSION HPN has protective effect on heart injury induced by hypobaric hypoxia in mice. Its mechanism may be related to the amelioration of energy metabolism,alleviation of oxidative stress as well as inhibition of myocardial apoptosis.     

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??OBJECTIVE To evaluate the benefits and risks of different durations of dual anti-platelet therapy after percutaneous coronary intervention with drug-eluting stent in patients with coronary disease. METHODS PubMed, Embase, Cochrane Library, CBM, CNKI, VIP, Wanfang Data, Clinical Trials and ICTRP were searched for randomized controlled trials(RCTs) comparing different DAPT durations after PCI with DES in patients with coronary disease. Risk of bias was assessed with the tool recommended by Cochrane Collaboration. Network Meta-analysis was performed with WinBUGS 1.4.3 and STATA 12.0. RESULTS A total of 3 230 articles were found and 10 RCTs including 31 643 patients were systematically reviewed. Network Meta-analysis showed there was no significantly difference among the 6 different durations in the rates of stent thrombosis, myocardial infarction, all-cause mortality and major bleeding, except that the durations of 6 months [OR 10.56,95%CI(1.62, 9.17)] and 12 months [OR 4.05,95%CI(1.01, 11.46)]were different with 30 months in the rate of stent thrombosis. Ranking of cumulative probabilities showed that 30 months and 36 months of DAPT had the lowest risk of stent thrombosis and myocardial infarction, meanwhile duration of 3 months and 6 months had the lowest risk of all-cause mortality and major bleeding. CONCLUSION Clinicians should assess the risks of stent thrombosis and bleeding according to patient??s individual condition and determine the optimal duration of DAPT. It is considered feasible to appropriately extend the duration of DAPT in patients at lower bleeding risk.     

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??OBJECTIVE To study the effects of ganoderma spore oil(GSO) on behavior of the mice with chronic unpredictable mild stress (CUMS) and its possible neurophysiology mechanisms. METHODS Thirteen different kinds of chronic unpredictable mild stress were given to the male BALB/C mice for establishing the mouse model of depression. The mice were treated with GSO at 3 doses (850, 283, 141.5 mg??kg^{- 1}??d^-1) or vehicle [(oil) or fluoxetine (10 mg??kg^{- 1}??d^-1)] by oral administration from the 3rd week. After 2 weeks administration, the mice was evaluated by behavioral tests, and the contents of hippocampal glutamate (GLU) and ??-amino butyric acid (GABA) were analyzed by reversed phase high performance liquid chromatography (RP-HPLC). The contents of hippocampal brain derived neurotrophic factor (BDNF) were measured by ELISA kit. RESULTS Compared with model group, GSO increased the body weight, sucrose preference rate and open field test score, shortened the immobility time in the tails suspension test and forced swimming test in the depression mice (P<0.05 or P<0.01). Meanwhile, GSO significantly decreased the contents of GLU (P < 0.01 ) and increased the contents of BDNF(P<0.01), and the contents of GABA did not changes (P>0.05) in the hippocampus. CONCLUSION GSO shows obvious anti-depressant effect on depressant model mice. The antidepressant effect of GSO may be related to decreasing GLU contents and increasing BDNF contents.     

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??To review the research progress of small molecule tyrosine kinase inhibitors (TKIs). Literatures on tyrosine kinase inhibitors which have already been successfully marketed or are in development were sorted and summarized according to the targets and related cancers. Through binding to catalytic domain of intracellular tyrosine kinase, small molecule TKIs inhibit the catalytic activity specifically and block cell proliferation signals, and some of them have already been used in cancer therapy or are in different stages of clinical development, showing good prospects.     

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??Berberine belongs to the quaternary ammonium type isoquinoline alkaloid known as berberinum. In traditional Chinese medicine, it has long been used to treat infections of the gastrointestinal tract disease caused by bacteria.Recent studies found that berberine has a good anti-tumor effect,but it is difficult pass through the cell membrane due to its poor fat-soluble,which limit its clinical application. By modifing chemical structure,some researchers have investigated the relationship between activity effects and structures of berberine and its derivatives, and have found a series of anti-tumor activity enhanced berberine derivatives.In this review, the recent research progress of the anti-tumor effects and their mechanisms of berberine and its derivatives are focused, which may provide reference for the design and development of anticancer drugs based on the structure of berberine     

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??OBJECTIVE To investigate the effects of microwave radiation on learning and memory abilities in mice,and to study pilose antler peptide??s intervention. METHODS Fifty mice were divided into five groups randomly, designated as control group, radiation group, pilose antler peptide (25, 50, and 100 mg??kg^-1) groups. Learning and memory impairment model in mice was established by microwave radiation of 2 450 MHz average surface power, 10.0 mW??cm^-2 for 90 min every day for 28 d .The radiation rats were treated with low-, mid-, and high-dose (25, 50, and 100 mg??kg^-1) pilose antler peptide by sc injection for 28 d. The learning and memory ability of mice was determined by avoiding darkness experiment and Y maze experiment.The contents of S100B, tumor necrosis factor-??(TNF-??), interleukin-10(IL-10), malondialdehyde (MDA), and nitric oxide(NO) in the brain of mice were determined respectively after the behavioral experiments. RESULTS Compared with control group, radiation group could shorten the latency of avoiding darkness experiments, increase the numbers of errors both in avoiding darkness experiment and in Y maze experiment. Radiation group could rise the contents of S100B ,TNF-??, IL-10, MDA and NO in the brain of mice (P<0.05 or P<0.01). Compared with radiation group, pilose antler peptide (50, 100 mg??kg^-1) groups could lengthen the latency of avoiding darkness experiments, significantly shorten the numbers of errors both in avoiding darkness experiment and in Y maze experiment, and reduce the contents of S100B ,TNF-??, MDA and NO, increase the content of IL-10 in the brain of mice (P<0.05 or P<0.01). CONCLUSION Pilose antler peptide could significantly perfect the learning and memory ability of mice exposed to microwave radiation. The mechanism may be related to its anti-oxidative actions by anti-inflammatory action , further lowering neurotoxic effects of NO.     

大黄酚对铅中毒小鼠学习记忆能力的影响

目的:研究大黄酚对铅中毒小鼠学习记忆能力及组织内铅水平的改善作用,并探讨其可能的作用机制.方法:采用连续8 dip7 mg?kg-1醋酸铅造成铅中毒小鼠模型,采用跳台实验,观察ip大黄酚(10.0,1.0,0.1 mg? kg-1)对铅中毒模型小鼠记忆能力的改善作用,并于大黄酚治疗14 d后测定小鼠心、肝、脾、肾内铅水平,同时测定小鼠肝、肾组织内丙二醛(MDA)含量及超氧化酶歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活力.结果:连续8 dip7 mg?kg-1醋酸铅造成铅中毒小鼠模型学习记忆障碍,使小鼠心、肝、脾、肾内铅水平显著升高,小鼠肝、肾内SOD和GSH-Px活性显著降低,使MDA含量增加;而连续ip大黄酚14 d治疗后,可不同程度提高小鼠铅中毒后学习记忆能力,与模型组相比大黄酚治疗组均可显著降低心、肝、脾、肾铅含量(P＜0.01);与模型组相比大黄酚10.0,1.0 mg?kg-1治疗组可显著升高肝、肾内SOD和GSH -Px的活性,降低MDA含量(P＜0.01),而大黄酚0.1 mg?kg-1治疗组可显著升高肾内SOD和GSH-Px的活性,降低MDA含量(P＜0.01),对肝内SOD,GSH-Px的活性及MDA含量无显著影响.结论:大黄酚可显著改善铅中毒小鼠学习记忆能力,降低铅中毒小鼠各组织的铅水平,提高小鼠肝、肾组织内抗氧化酶的活性,改善铅中毒造成的脂质过氧化.     

CTX对雄性小鼠生殖功能的影响及杨梅素的干预研究

目的:观察环磷酰胺(Cyclophosphamide,CTX)对雄性小鼠生殖功能的影响,研究杨梅素(Myricetin)的保护作用及作用机制。方法:腹腔注射环磷酰胺50 mg/kg连续7 d,建立雄性小鼠生精障碍模型,造模第2 d始,分别灌胃杨梅素100 mg/kg、200 mg/kg、400 mg/kg组,连续30 d。观察雄性小鼠的睾丸指数、精子密度、精子畸变率,血清中丙二醛(MDA)、睾酮(T)、一氧化氮(NO)的含量,睾丸组织匀浆的Na~+-K~+-ATP酶、碱性磷酸酶(AKP)、琥珀酸脱氢酶(SDH)、还原型谷胱甘肽(GSH)、NO等指标活力。结果:与正常对照组比较,模型对照组睾丸指数、精子密度、Na~+-K~+-ATP酶、AKP、SDH活性、GSH、T含量明显降低,精子畸形率、MDA、NO含量明显增高。与模型组比较,杨梅素200 mg/kg、400 mg/kg组的精子密度明显增加、精子畸变率明显降低,睾丸组织中Na~+-K~+-ATP酶、AKP、SDH及GSH水平明显增加,NO含量明显降低,杨梅素100 mg/kg、200 mg/kg、400 mg/kg组血清中MDA、血清和睾丸组织中NO含量均明显降低、血清中T含量增高,杨梅素400 mg/kg组睾丸指数明显升高。结论:杨梅素对环磷酰胺引起的小鼠生精功能障碍有一定防治作用,作用机制与抗氧化、降低NO水平进而维持睾酮水平有关。     

六味地黄汤及其加味组方对生精障碍模型大鼠的促生精作用研究

目的:考察六味地黄汤及其加味组方对生精障碍模型大鼠的促生精作用。方法:用醋酸棉酚造成大鼠生精障碍模型后,以分煎和合煎的六味地黄汤及其加味方灌胃治疗,以市售六味地黄丸和甲基睾酮分别为对照和阳性对照,考察实验各组精子的数量和质量。结果:六味地黄汤分煎组、合煎组、市售组、加味组方各组及阳性对照组的精子数与模型组有显著性差异(P<0.05),各治疗组的精子质量(活力)也显著优于模型组。结论:六味地黄汤及其加味组方对生精障碍大鼠均有显著的促进生精作用,并可显著提高大鼠精子的质量。     

蕨菜黄酮对环境胁迫因子(铅)引起的小鼠细胞氧化的影响

目的:研究饲喂蕨菜黄酮对小鼠在铅胁迫下细胞氧化的影响。方法:采用硫代巴比妥酸法(TBA法)测定小鼠肝组织和血清中MDA的相对含量以及改良的邻苯三酚自氧化法测定肝组织中SOD活性。结果:铅胁迫下,小鼠肝组织中SOD活性显著降低(P<0.01),肝组织和血清中MDA相对含量显著升高(P<0.01)。饲喂蕨菜黄酮组小鼠肝组织SOD活性回升,当饲喂蕨菜黄酮量为150mg/kg体重时,肝组织SOD活性上升明显(P<0.05),饲喂蕨菜黄酮量达到300mg/kg体重时,肝组织SOD活性上升显著(P<0.01),并且达到正常水平;饲喂蕨菜黄酮组小鼠肝组织和血清中MDA的相对含量均降低。肝组织中,Pb 50组与单独铅胁迫组相比,MDA的相对含量下降显著(P<0.05),Pb 150组与单独铅胁迫组相比MDA的相对含量下降极显著(P<0.01),饲喂蕨菜黄酮量继续加大时(300mg/kg体重),肝组织MDA的相对含量持续下降(P<0.01),比较Pb 50组与Pb 150组,显示后者MDA含量下降幅度大(P<0.01),而Pb 150组与Pb 300组相比,后者MDA含量下降幅度大(P<0.01)。血清中,Pb 50组与单独铅胁迫组相比,MDA的相对含量下降但不显著(P>0.05),Pb 150组与单独铅胁迫组相比MDA的相对含量下降显著(P<0.05),Pb 300组与单独铅胁迫组相比MDA的O.D值下降极显著(P<0.01),比较Pb 300组与Pb 150组,Pb 300组血清中MDA含量进一步下降,二者差异达到极显著水平(P<0.01)。结论:提示饲喂蕨菜黄酮的量与小鼠肝组织和血清中MDA含量的下降呈一定程度的正相关。说明在本实验条件下,饲喂蕨菜黄酮有明显改善小鼠机体代谢合成,促进肝组织细胞的正常生长,具有清除体内氧自由基功能以及增强机体抗氧化功能等保健作用。     

温肾生精饮治疗生精障碍的实验研究

目的：临床上利用温肾生精饮治疗男性生精障碍,已取得了显著疗效,本实验利用生精障碍小鼠模型探讨温肾生精饮促进精子生成的可能作用机制。方法：利用环磷酰胺对昆明小白鼠建立生精上皮损伤模型,对造模后的小鼠分别灌服温肾生精饮或等量生理盐水,灌服20天后检测小鼠附睾尾精子质量,并进行睾丸组织学检查和超微结构观察。结果：中药组小鼠附睾活精子的百分率和精子密度显著高于模型组;中药组小鼠睾丸生精小管结构规则,生精上皮排列整齐,上皮层数较模型组显著增多;中药组小鼠睾丸的超微结构与模型组相比,间质细胞胞质内脂滴较少,线粒体较多,溶酶体较多,间质细胞显示出旺盛的分泌能力。结论：温肾生精饮提高了间质细胞的分泌功能,维持着睾丸生精小管内高水平睾酮,以增加生精上皮层数,促进精子发生,提高正常精子的比率。     

鳗鱼油胶囊对三氯化铝致小鼠记忆障碍的改善作用

目的：研究鳗鱼油对三氯化铝致小鼠脑记忆障碍模型的改善作用。方法：以跳台、避暗、Y型迷宫和日式迷宫试验,对三氯化铝致脑记忆障碍模型小鼠的学习和记忆进行检测。结果：鳗鱼油能明显减少跳台试验中三氯化铝（AlCl3)脑记忆障碍模型小鼠受电击次数,延长24h后的潜伏期,减少错误次数和错误频率;在避暗试验中,明显延长进入暗室的潜伏期;在Y型迷宫试验中,显著缩短到达安全区所需时间;在日式迷宫试验中,可明显缩短小鼠到达终点的时间,同时减少错误次数。结论：与模型组相比较,鳗鱼油能明显改善AlCl3致小鼠脑记忆障碍,对其学习和记忆的获得、巩固和再现均有明显的增强作用,且低、中、高剂量组间呈现明显的量效关系。     

升白丸对环磷酰胺致小鼠白细胞减少症的影响

目的：观察升白丸对环磷酰胺致小鼠白细胞减少症的影响。方法：利用环磷酰胺ip造成小鼠白细胞减少症模型，口服升白丸进行治疗，对模型小鼠外周血白细胞总数、骨髓有核细胞数及胸腺、脾脏指数进行检测。结果：升白丸可明显升高模型小鼠的外周血白细胞总数、骨髓有核细胞数及胸腺、脾脏指数。结论：升白丸具有明显升白作用。     

刺玫果对烹饪油烟引起小鼠骨髓PCE微核率增高的影响

本文研究了刺玫果对烹饪油烟引起的小鼠骨髓嗜多染红细胞微核率的影响。结果表明,烹饪油烟各实验组的小鼠骨髓嗜多染红细胞微核率明显高于阴性对照组(P<0.01),且呈现剂量-反应关系,刺玫果明显降低烹饪油烟诱发的微核率。     

三康胶囊对环磷酰胺所致免疫功能低下小鼠的保护作用

目的:观察三康胶囊对环磷酰胺所致免疫低下小鼠免疫功能的影响。方法:用环磷酰胺(100 mg/kg,ip)制备小鼠免疫低下模型,灌胃给予不同剂量的三康胶囊(250 mg/kg,500 mg/kg)和阳性对照药贞芪扶正胶囊(500mg/kg),观察小鼠外周血白细胞计数、免疫器官重量、单核吞噬细胞吞噬指数、血清溶血素水平和脾脏淋巴细胞转化指数。结果:对免疫功能低下小鼠,三康胶囊能增加外周血白细胞数量及免疫器官指数,增强单核吞噬细胞的吞噬能力,提高血清溶血素水平和脾脏淋巴细胞转化指数。结论:三康胶囊能够增强环磷酰胺所致免疫功能低下小鼠非特异性免疫和特异性免疫功能,对免疫失衡机体具有保护作用。