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??OBJECTIVE To prepare inclusion complex of SBE₇-??-CD with trimethoprim(TMP) and optimize the preparation process, to evaluate the products by structural characterization and molecular simulation. METHODS The TMP/SBE₇-??-CD inclusion complex was prepared by the ultrasound-freeze-dry method and the preparation process was optimized by Box-Behnken Design-response surface method(BBD-RSM). Inclusion complex was characterized by FT-IR, DSC, XRPD and ¹H-NMR. Molecular docking method was used to simulate 3-dimensional conformations of the inclusion complex and the binding energy was calculated. The dissolution and stability were tested. RESULTS The optimum conditions of TMP/SBE₇-??-CD inclusion complex were: temperature(52 ??), time(45 min), and the ratio of SBE₇-??-CD and TMP(mol/mol, 1.7??1). All characterizations(FT-IR, DSC, XRPD and ¹H-NMR) indicated the formation of TMP/SBE₇-??-CD inclusion complex. The best 3-dimensional docking conformation was consistent with the characterizations, and the binding energy was -9.015 kcal??mol^-1. The TMP dissolution rate of the inclusion complex increased significantly, the hygroscopicity is strong. CONCLUSION The preparation process of TMP/SBE₇-??-CD inclusion complex optimized by BBD-RSM is reasonable and feasible. The characterizations of inclusion complex are reliable. The molecular simulation is corresponded to the characterized results and provided reliable theoretical basis for inclusion experiments.     

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??OBJECTIVE To investigate the rheological property of Kappa carrageenan and the effects of standing time, concentration, and temperature on the viscosity of carrageenan solution.METHODS The fluid type was determined by fitting the rheological profiles to Power law model.The effect of temperature on the rheological behavior was investigated according to the viscous flowactivation energy(E??) which was calculated by the Arrhenius formula. RESULTS The viscosity of carrageenan solution decreased with the increase of shear rate and temperature,whereas it increased with the concentration. There existed a positive correlation between E?? and concentration. Furthermore, the effects of different cations on the viscosity were also studied and the results indicated that the viscosity increased significantly with the ionic concentration, especially that of potassium chloride.CONCLUSION Carrageenan was inferred to be pseudo-plastic fluid which hasthe character of shear thinning effect and its viscosity is significantly affected by cationic species.     

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??Positron emission tomography(PET) is a nuclear imaging technology, which can accurately determine the local function and the biochemical course of organs or tissues of human body, so PET technology has an important role in the diagnosis of tumors and other diseases. ¹⁸F-FDG has been used in oncology for differentiating of benign tumors from malignant tumors, detecting and staging of malignant tumors. However, ¹⁸F-FDG has low specificity, no uptake in some tumor cells but appreciable uptake in inflammation. Therefore designing and synthesizing tumor imaging agents with high specificity and sensitivity is necessary and exigent. In view of the fact that the metabolism of protein is also increased in tumor tissues in addition to the metabolism of glucose, developing new amino acid imaging agents labeled by positron emission nuclide may have quite large potential.     

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??OBJECTIVE ??-Conotoxin LtIA (??-CTX LtIA, LtIA) is a specific inhibitor of ??3??2 nicotinic acetylcholine receptors (nAChRs) from Conus litteratus, a marine snail native to Hainan. The aim of this study was to evaluate the analgesic activity of ??-CTX LtIA. METHODS The analgesic effect of ??-CTX LtIA on pain models was evaluated using mice hot-plate and tail-flick models by intracerebroventricular (icv) injection. RESULTS In tail-flick test, the maximum analgesia percentage (PMAP) was 37.74% at 15 min after LtIA administration by icv injection with dose of 0.2 nmol per mouse. While in hot-plate test, PMAP was 48.81% at 60 min after LtIA administration by icv injection with same dose of 0.2 nmol per mouse. ??-CTX LtIA showed good analgesic activity in two pain models. CONCLUSION ??-CTX LtIA exhibits good analgesic activity by specific interaction with ??3??2 nAChRs subtype. These RESULTS have great significance for the research and development of LtIA painkiller in the future.     

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??OBJECTIVE To rapidly identify the related substances in doxycycline hyclate tablets and investigate the possible degradation pathways of doxycycline hyclate solution by 2D-LC-IT-TOF/MS. METHODS Firstly,the chromatography was carried out using the 1stD InertSustain^TM C₁₈(4.6 mm??150 mm,5 ??m) column and a mobile phase containing a mixture of buffer solution(0.25 mol??L^-1 ammonium acetate solution-0.1 mol??L^-1 ethyldiaminetetraacetic acid disodium: triethyiamine=100??10??1)-acetonitrile(85??15). Solution pH was adjusted to 8.8 with glacial acetic acid. Then a InertSustain^TM C₁₈(2.1 mm??50 mm,2 ??m) column was used with 0.1% formic acid-water as mobile A and 0.1% formic acid-acetonitrile as mobile B in a gradient elution mode in 2ndD. Electrospray ionization (ESI) source was tested in both positive and negative ion modes. Nebulized gas flow was 1.5 L?? min^-1. Dry gas flow was 10 L?? min^-1. The desolvation tube temperature was kept at 200 ??. Related substances were characterized according to multi-level MS behaviors. The 2D-LC-IT-TOF/MS method was employed to identify the structures of impurities in forced degradation solutions and illuminate the degradation pathways of doxycycline hyclate solution. RESULTS A total of eight related substances were detected in doxycycline hyclate tablets. Four of them had a content of more than 0.1%, and their structures were identified to be 4-epidoxycycline, metacycline, ??-epidoxycycline and 2-acetyl-2-decarbamoyldoxycycline. The solution of doxycycline hyclate easily degraded under alkaline condition and generated an open loop compound taken off the keto group. The solution was sensitive to heat, and generated 4-epidoxycycline with a little amount of 4-epi-6-epidoxycycline; but the solution was stable under illumination and acidic condition. CONCLUSION The established method is suitable for rapid identification of impurities in doxycycline hyclate, which can be applied as a useful analytical tool for quality control and drug process optimization of doxycycline hyclate.     

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??OBJECTIVE To evaluate the compatibility of 3-layer coextrusion (powder-liquid) infusion bag with lactose azithromycin powder and sodium chloride injection. METHODS The sterile powder of lactose azithromycin was dispensed in different infusion bags and the turbidities were observed after the accelerated test. The dissolve degree of packaging materials and additives, including infusion membrane and inner cap, in different acid, alkali and polar solvent were studied. The extent of migration of packaging material components into the drugs were researched after the lactose azithromycin powder and sodium chloride injection were dispensed in the 3-layer coextrusion (powder-liquid) infusion bag. RESULTS Compared with the four kinds of common membrane, the membrane material we chosed was the best and the turbidities of liquid were meet the specification. In the extraction studies of different test solution, the antioxidant was not checked out, and the content of both Mg and Al was less than 0.05 ??g??mL^-1. In the security evaluation of migration, the maximum migration, the biggest one-day intake value and the accumulated maximum intake of additives in the special infusion bag were far lower than its toxicology statistics. CONCLUSION The quality of 3-layer coextrusion (powder-liquid) infusion bag is good. The migration and adsorption degree of of packaging material are tested to make sure the packaging material could ensure the drug quality and stability. The compatibility of 3-layer coextrusion (powder-liquid) infusion bag with drug is nice.     

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??OBJECTIVE To interrogate differential sensitivity of ??-conotoxin TxID on stoichiometry of ??3??4 nicotinic acetylcholine receptors(nAChRs). METHODS Oocytes of Xenopus laevis were used to express rat ??3??4 nAChRs with different stoichiometries by altering ??3:??4 RNA injection ratios of 1:1, 1:10 or 10:1. Sensitivity of ??-conotoxin TxID on these different stoichiometry receptors were evaluated and compared. RESULTS The three stoichiometry receptors of ??3??4 nAChRs were expressed in oocytes successfully. ??-Conotoxin TxID showed differential sensitivity on ??3??4 nAChR stoichiometries. Inhibition of 1:10 injection ratio by TxID was similar with regular 1:1 ??3??4 nAChRs within 2-fold difference. While potency of 10:1 injection ratio by TxID decreased 5-fold significantly comparing with 1:1 ??3??4 nAChRs. CONCLUSION ??-Conotoxin TxID exhibits distinct sensitivity on different stoichiometry of ??3??4 nAChRs, which could reflecting different stoichiometries of ?? and ?? subunits. The RESULTS would be helpful for elucidation of structure and physiology function of ??3??4 nAChRs.     

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??OBJECTIVE To establish an HPLC-ELSD method for determination of mesaconine.METHODS Gemini C₁₈ 110A column (4.6 mm??150 mm,5 ??m) was adopted with the mobile phase consisting of methanol-0.1%TFA (25:75) at the flow rate of 0.6 mL??min^-1. The detector was Alltech ELSD 2000ES, with the drift tube temperature of 90 ?? and the airflow velocity of 2.3 L??min^-1. RESULTS Under this conditions, mesaconine and the related substances were well separated. The linear range of mesaconine was 0.40-0.60 mg??mL^-1 (r=0.999 6). RSD of injection precision and repeatability test were 0.04% (n=5) and 0.7% (n=6), the intra day and inter day precision RSD were 0.4% and 0.6%, respectively. The average recovery was 101.0% (RSD=1.1%, n=9). CONCLUSION The established method was specific, simple, accurate, and suitable for determination of mesaconine.     

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??OBJECTIVE To study the flavonoid glycosides of Urena lobata. METHODS Compounds were isolated and purified using various column chromatographies such as D101 macroporous adsorption resin, silica gel, Sephadex LH-20, and prep HPLC. Their structures were identified on the basis of their physicochemical properties and various spectroscopic experiments, including HRESIMS, ¹H-NMR, ¹³C-NMR, HSQC, and HMBC. RESULTS Ten flavonoid glycosides were obtained from the n-BuOH extract of U. lobata including quercetin-3-O-??-D-glucopyranosyl-(1??2)-??-D-galactopyranoside(1), kaempferol-3-O-??-D-glucopyranosyl-(1??2)-??-D-glucopyranosyl-7-O-??-L-rhamnopyranoside(2), quercetin-3-O-??-D-apiofuranosyl-(1??2)-??-D-glucopyranosyl-7-O-??-L-rhamnopyranoside(3), kaempferol-4'-O-??-D-apiofuranosyl-3-O-??-D-glucopyranosyl-7-O-??-L-rhamnopyranoside(4), kaempferol-3-O-??-D-apiofuranosyl-(1??2)-??-D-glucopyranosyl-7-O-??-L-rhamnopyranoside(5), quercetin-3-O-??-D-glucopyranosyl-7-O-??-L-rhamnopyranoside(6), quercetin-3-O-??-D-glucopyranosyl-(1??2)-??-D-glucopyranoside(7), kaempferol-3-O-??-L-rhamnopyranosyl-(1??6)-??-D-glucopyranosyl-(1??2)-??-D-glucopyranoside(8), kaempferol-3-O-??-D-glucopyranosyl-(1??2)-[??-L-rhamnopyranosyl-(1??6)]-??-D-glucopyranoside(9) and kaempferol-3-O-??-D-glucopyranosyl-(1??2)-??-D-glucopyranoside(10). CONCLUSION Compounds 1-3 and 6-10 are firstly obtained from U. lobata.     

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??OBJECTIVE To investigate the mechanism and the protective effect of arginyl-fructosyl-glucose(AFG) on the kidney of diabetic nephropathy(DN) mice and the correlation between TGF-??1 and collagen ??. METHODS Twelve mice were randomly selected as control group; all others were induced by streptozotocin (STZ). The mice were randomly divided into 5 groups, each of which has 12 mice, DN model group, metformin group, 10,20,40 mg??kg^-1 AFG group; each group was treated for 5 weeks, during when mice were weighed weekly and blood glucose concentration were measured. At the end of 5 weeks, the final body weight and the renal index of the mice was calculated; 24 h urinary protein and blood biochemical indexes were recorded (TC, TG, HDL-C, SCr, BUN, SOD, GSH, CAT, T-AOC); the pathological changes of the kidney was observed using HE staining. The expressions of TGF-??1 and collagen ?? mRNA in kidneys were detected by real-time fluorescence quantitative PCR (RT-PCR). RESULTS The levels of urinary protein, TC, TG and HDL-C in the AFG group were significantly lower than those in the control group. The contents of SOD, GSH, CAT and T-AOC in the AFG-treatment group were significantly higher than those in the control group. Pathological condition was relieved; the mRNA expression of TGF-??1 and collagen ?? in the kidneys of each dose group was significantly lower than that of the control group. CONCLUSION AFG protects the kidneys by reducing the total amount of 24 h urinary protein, decreasing renal pathological damage and decreasing the expression of TGF-??1 and collagen ?? genes in kidney.     

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??OBJECTIVE To study the effects of fibroblast growth factor-21(FGF-21) on acute inflammation induced by lipopolysaccharide (LPS)and its mechanism. METHODS Mouse model of acute inflammation was established by injection of LPS and treated with FGF-21 at high, medium and low doses, the pathological changes were detected with HE staining, the expression level of TNF-??, IL-1??, IL-6 and IL-17 in serum and peritoneal macrophage were determined by ELISA and Real-time PCR. NF-??B p65 in macrophage cells was analyzed by confocal laser scanning microscope and Western-blotting. RESULTS FGF-21 treatment reduced lung damage and inflammatory cell infiltration and decreased the expression level of TNF-??, IL-1??, IL-6 and IL-17 in both serum and peritoneal macrophage. The results of confocal laser scanning microscope and Western-blotting both showed that FGF-21 could inhibit NF-??B transferring to the nucleus. CONCLUSION FGF-21 could regulate the immune system by acting on macrophage. Relieving the inflammatory response in mice through NF-??B signal pathway may be one of the mechanisms FGF-21 regulating immune system.     

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??OBJECTIVE To investigate antagonistic activities of three isomers of ??-conotoxin TxIB on rat and human ??6/??3??2??3 nicotinic acetylcholine receptors (nAChRs). METHODS Three disulfide bond isomers were synthesized using Fmoc chemistry, which were identified by ultra performance liquid chromatography (UPLC)and confirmed by MALDI-TOF mass spectrometry. Rat and human ??6/??3??2??3 nAChRs were expressed in oocytes of Xenopus laevis, which were used to test the antagonistic abilities of the 3 isomers. RESULTS The three isomers of ??-conotoxin TxIB were synthesized successfully.The retention time of each isomer of ??-conotoxin TxIB was different each other significantly. The observed molecular masses of three isomers were the same, which were consistent with their theoretical molecular mass.Their hydrophilicity orders were globular > ribbon> bead. Both rat and human ??6/??3??2??3 nAChRs were expressed in oocytes well. Inhibition of three isomers of ??-conotoxin TxIB on rat and human ??6/??3??2??3 nAChRs were evaluated respectively. Among the three isomers of TxIB, the activity of the globular isomer was the most potent one, which had almost same activity at rat and human ??6/??3??2??3 nAChRs with corresponding IC₅₀ of 28.2 and 32.0 nmol??L^-1respectively. However, the other two isomers, ribbon and bead isomers displayed little antagonistic effect on both rat and human ??6/??3??2??3 nAChRs only with an IC₅₀ of ??10 ??mol??L^-1. CONCLUSION The synthesized globular isomer of ??-conotoxin TxIB in this work has a high selectivity and potent antagonistic activity on rat and human ??6/??3??2??3 nAChRs, which would be helpful for its new drug development.     

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??OBJECTIVE To investigate the effect of ??-secretase inhibitor DAPT in inflammation-induced brain white matter injury in neonatal mice.METHODS Sixty C57BL/10J neonatal mice are randomly divided into control group, control+DAPT (10 mg??kg^-1) group, inflammation (LPS) group, LPS+DAPT group (inflammation exposure after 10 mg??kg^-1 DAPT treatment). All neonatal mice were killed and brain was removed to the following observation and detection:at P5, the mRNA expression variation of IL-1??, IL-8,TNF-??,Hes1 and NICD by Real-time PCR methods. Oligodendrocytes were identified by immunofluorescence staining. Myelin basic protein (MBP) protein expression was detected by Western blot assay.RESULTS LPS group showed brain injury characterized by inhibition of brain development. There were significant differences in mRNA expression of IL-1??, IL-8, TNF-??, Hes1 and NICD between LPS+DAPT group and LPS group (P<0.05), and the mRNA expression of IL-1??, IL-8,TNF-??,Hes1 and NICD in inflammation-treated were significantly increased than control group (P<0.05). The results showed more expression of MBP in LPS+DAPT group compared with LPS group (P<0.05). Compared with the blank control group, which was obviously decreased after 48 h of inflammation (P<0.05).CONCLUSION Inflammation leads to abnormal of notch signal expression in neonatal mice, and which is shows inflammation involved in brain damage.Its mechanism is probably associated with the maturation of oligodendrocytes.     

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??OBJECTIVE To develop an high-performance liquid chromatography (HPLC) method with diode array detector (DAD) for determination of acarbose in acarbose tablets. METHODS An Alltima C₁₈ column (4.6 mm??250 mm,5 ??m) was used for the separation, with acetonitrile-10 mmol??L^-1 ammonium dihydrogen phosphate (containing 0.04% sodium 1-octanesulfonate, adjusted pH to 3.3 with H₃PO₄) (15??85) as the mobile phase at the flow rate of 0.8 mL??min^-1. The detection wavelength was set at 200 nm and the column temperature was maintained at 30 ??. RESULTS The method showed good linearity with a correlation coefficients (r) of 1.000 0. The specificity study demonstrated satisfactory resolutions between acarbose, other ingredients in the drug and forced degradation products. The precision and stability were satisfactory with the relative standard deviations (RSDs) of 0.088% and 0.22%, respectively. The average spiked recovery was 98.49% with RSD of 0.24% (n=9). The content of acarbose in four batches of acarbose tablets was 51.69, 51.75, 51.72 and 51.62 mg??tablet^-1, respectively. CONCLUSION The established method is accurate, simple and rapid, and can be utilized for determination of acarbose in acarbose tablets.     

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??OBJECTIVE To prepare cholesterol-PEG-modified amphotericin B liposomes and amphotericin B liposomes, and evaluate their encapsulation efficiency and pharmacokinetics. METHODS It was employed to prepare both kinds of liposomes with the film-evaporation, the dynamic light scattering techniques and Ultrafree-CL were used to determine the mean diameter and the encapsulation efficiency, respectively. Pharmacokinetics was evaluated in Wista rats. RESULTS Mean diameter of the liposomes was (115??20) nm,and the encapsulation efficiency was over 99.9%. Moreover, the pharmacokinetic study in rats indicated that cholesterol-PEG-modified liposomes could significantly increase the ??_max and AUC. CONCLUSION Amphotericin B liposomes prepared by film-evaporation method have high encapsulation efficiency, and cholesterol-PEG modification can prolong the circulation time of amphotericin B in vivo.     

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??OBJECTIVE To investigate the effects of interferon-??(IFN-??) and all-trans retinoic acid(ATRA) on multidrug resistance reversal effect and mechanism of human leukemia K562/ADM cells. METHODS The cytotoxicity and reversal times of IFN-?? and ATRA were detected by CCK-8 method. Apoptosis rate and cell cycle were detected by flow cytometry. PI3K, Akt and Bad mRNA were detected by RT-PCR method. Western blot method was used to detect the expression of PI3K, AKt, P-AKt and Bad protein.RESULTS The drug resistance of K562/ADM cells to adriamycin(ADM) was 54 times. ADM, respectively, with IFN-??, ATRA or combined application, the drug resistance of K562/ADM cells to ADM was 1.24, 2.34 and 8.14, respectively. The apoptosis rate of K562/ADM cells was significantly increased by using ADM 4 mg??L^-1alone or in combination with IFN-?? 2.5??10⁶ U??L^-1, ATRA 7.5 ??mol??L^-1, and the cell cycle was blocked in G₀/G₁ phase. PI3K mRNA and protein expression were significantly lowered, Akt mRNA and protein has no obvious change, Bad mRNA and protein expression are raised, phosphorylated Akt protein expression decreased, the expression is more obvious when the two drug combination. CONCLUSION IFN-?? and ATRA can reverse the multidrug resistance of K562/ADM cells, its mechanism may be the inhibition of the PI3K/Akt pathway.     

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??OBJECTIVE To study the chemical constituents from the aerial parts of Paris polyphylla var. chinensis. METHODS The compounds were isolated and purified from the 75% ethanol extract by chromatography on HPD100 macroporous resin, silica gel, and Sephadex LH-20 as well as semi-preparative HPLC. Their structures were elucidated on the basis of spectral data. RESULTS Eleven compounds were isolated and identified as corchionoside C (1), ??-ecdysterone (2), coronatasterone (3), kaempferol-3-O-??-D-galactopyranoside (4), astragalin (5), isorhamnetin-3-O-??-D-glucopyranoside (6), kaempferol-3-O-??-D-glucopyranosyl-(l??2)-??-D-galactopyranoside(7), isorhamnetin-3-O-??-D-glucopyranosyl-(l??2)-??-D-galactopyranoside (8), kaempferol-3-O-??-D-glucopyranosyl-(l??2)-??-D-glucopyranoside (9), isorhamnetin-3-O-??-D-galactopyranosyl-(l??6)-??-D-glucopyranoside (10), and isorhamnetin-3-O-??-D-gentiobioside (11). CONCLUSION Compounds 1 and 3-11 are isolated from this plant for the first time and compounds 1, 3-5 and 8-10 are isolated from Paris plants for the first time.     

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??OBJECTIVE To synthesize a novel cationic cholesterol derivative, N-(N??,N??-dimethyl)propyl succinic mono-cholesteryl mono-amide(DMAPA-CHEMS), and evaluate its feasibility as a none-viral gene vehicle. METHODS DMAPA-CHEMS and phospholipid were employed to produce liposomes by thin membrane dispersion method, and the morphology of liposomes was observed under transmission electron microscope. The average diameter and Zeta potential were determined by laser particle size analyzer. The combination to and protection for DNA were investigated via agarose gel electrophoresis. The uptake of FITC-labelled oligonucleotides into HepG2 cells was determined by flow cytometry, while the transfection promotion of liposomes for GFP-plasmid DNA was observed by inverted fluorescence microscope. RESULTS The obtained liposomes showed regular shape and uniform size, with a mean diameter of 94.0 nm and Zeta potential of 24 mV. The liposomes could combine DNA completely and protect DNA from the degradation of DNase I when the charge ratio of cationic cholesterol to DNA was more than 4. The cationic liposomes had low cytotoxicity. Although the uptake efficiency of liposomes made of DMAPA-CHEMS was lower than that of DC-Chol, the gene expression efficiency was higher. CONCLUSION DMAPA-CHEMS is a novel gene carrier with high transfection efficiency and low cytotoxicity, which has a great potential for application.     

N,N-���׻�-[N��,N��-˫(Ӳ֬����-1-�һ�)]1,3-�������ĺϳɡ����ʼ���Ϊ�װ����������Ӧ��

??OBJECTIVE To synthesize a novel cationic lipid,N,N-dimethyl-[N??,N??-di-(stearoyl-1-ethyl)] 1,3-diaminopropane (DMSP),and evaluate its feasibility as methotrexate(MTX) carrier. METHODS DMSP and phosphatidylcholine were employed to prepare liposomes by reverse phase evaporation method,and then MTX was entrapped by physical mixing. The entrapment efficiency was determined by ultracentrifugation,and its release ratio was evaluated by dialysis. The morphology of liposomes was observed under transmission electron microscope. The average diameter and Zeta potential were determined by laser particle size analyzer. MTT test was used to evaluate the cytotoxicity of liposomes as drug carrier and the inhibition of cancer cells growth. RESULTS The obtained liposomes showed regular shape and uniform size,with a mean Zeta potential of +(36.26??4.77)mV and average diameter of 120 nm. The liposomes had low hemolytic activity and cytotoxicity. With the help of DMSP the cationic liposomes achieved a very high entrapment efficiency for the hydrophilic drug MTX (91.50??1.02)%. The inhibition of the MTX liposomes on cancer cells growth was much higher than that of MTX solution. CONCLUSION DMSP is a novel cationic lipid with low cytotoxicity and high entrapment efficiency,which has a great application potential in drug delivery system.     

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??OBJECTIVE To determine the contents of six oligosaccharides, i.e. glucose, fructose, sucrose, 1-kestose, nystose, 1^F-fructofuranosylnystose, in the roots of Morinda officinalis in different growing periods by HPAEC-PAD method. METHODS The separation was performed on Hamilton RCX-10 column(4.1 mm??250 mm, 7 ??m) with gradient elution using mobile phase composed of 100 mmol??L^-1 NaOH(A)and mixture of 100 mmol??L^-1 NaOH and 500 mmol??L^-1 NaOAc (B) at a flow rate of 0.8 mL?? min^-1 and the temperature was kept at 35 ??. The injection volumn was 25 ??L. The gradient elution program was as follows: 0-5 min,1%B;5-15 min,1%-9% B;15-20 min,9%-12% B;20-25 min,12%-16% B;25-40 min,16%-50% B;40-43 min,50% B;43-46 min,50%-1% B. RESULTS With the increase of growing periods, the content of monosaccharide progressively reduced while that of sucrose gradually increased. The contents of oligosaccharides with polymerization degree of 3 or greater, including 1-kestose, nystose, and 1^F-fructofuranosylnystose, slowly increased during 1-5 years, then slowed down. CONCLUSION The contents of six oligosaccharides in the roots of Morinda officinalis vary greatly in different growing periods, indicating that the biosynthesis of oligosacchride in Morinda officinalis is related to growing periods. The result provides a reference for determination of appropriate collection time.