Penetration of radiocalcium at the margins of resin and glass ionomer dentine bonding agents in primary and permanent teeth

OBJECTIVES: The purpose of this study was to compare the leakage of three resin dentine bonding agents (Prime and Bond, Scotchbond Multi-Purpose, Probond) and a glass ionomer dentine bonding agent (GC Fujibond LC), in cervical cavities prepared in primary and permanent molar teeth restored with a hybrid composite resin (Tetric). METHODS: Cervical cavities without a bevel at the cavo-surface margins were prepared in the buccal and lingual surfaces of extracted primary and permanent molar teeth. After being restored, the teeth were stored for 1 week in a saline solution at 37 degrees C and then thermally cycled between 5 and 55 degrees C. Marginal leakage was determined subsequently using a radioactive isotope containing 45Ca and an autoradiographic technique. RESULTS: The results revealed that there were no statistically significant differences in microleakage of the bond between permanent and primary teeth dentine and Fuji Bond LC and Probond dentine bonding agents. The difference between permanent and primary teeth groups for gingival values of the Prime and Bond 2.1 group U=22.5, p=0.0355 and the Scotchbond Multipurpose group U=24.0, p=0.0406 were statistically significant. There were no significant differences between the occlusal and gingival microleakage values in either primary or permanent teeth with Prime and Bond 2.1, Fuji Bond LC and Probond except the difference at Scotchbond Multipurpose in primary teeth. For primary teeth gingival margins, none of the bonding systems were significantly different from the control group. CONCLUSIONS: These results indicate that although no statistically significant differences were found between test and control group values, the use of Fuji II LC in cervical cavities with cementum margins in primary teeth would provide the best resistance to microleakage among the test materials while the use of Scotchbond Multi-Purpose would provide the best resistance to microleakage in cervical cavities with cementum margins in permanent teeth.     

Comparison of microleakage for three one-bottle and three multiple-step dentin bonding agents.

PURPOSE: This in vitro study compared the ability of several recently introduced 1-bottle adhesives to their preceding multiple-step dentin bonding agents in reducing microleakage around Class V composite restorations. MATERIAL AND METHODS: Facial and lingual Class V cavities were prepared in 48 molar teeth, which were randomly divided into 6 equal groups. Three groups used the fourth-generation multiple-step systems, and 3 used the fifth-generation single-bottle adhesives. All cavities were restored by composite and subjected to thermocycling and intermittent occlusal loading. After immersion in 0.5% basic fuchsin, the teeth were cut faciolingually in 5 consecutive sections and evaluated for dye penetration using a binocular stereomicroscope. RESULTS: There was no statistically significant difference in the degree of microleakage comparing the adhesive's generation and manufacturer and the location of the cavity margins. A significant interaction was found between margin location and manufacturer, which implied that the sealing capacity at the enamel and cementum margins was material-specific. CONCLUSION: Scotchbond Multi-Purpose and Single Bond adhesives provided the best seal for enamel margins, and One-Step and Optibond FL adhesives were best for cementum margins.     

Influence of flowable liner and margin location on microleakage of conventional and packable class II resin composites

This in vitro study evaluated gingival wall microleakage in packable and microhybrid conventional composite restorations with and without a flowable composite liner. Each group was evaluated with gingival margins situated in both enamel and cementum/dentin. Two hundred and forty Class II cavities were prepared in extracted third molars, half with gingival margins in enamel and half with margins in dentin/cementum. In groups of 30, restoration was undertaken with packable alone (3M Filtek P60), conventional alone (3M Z250), packable plus flowable liner (3M Filtek Flow) and conventional plus flowable liner. All used 37% phosphoric acid etch and Scotchbond 1 (3M) as the bonding system. After restoration, the teeth were thermocycled (between 5 degrees C, 37 degrees C and 60 degrees C) 1,500 times, soaked in 0.1% methylene blue, sectioned and microleakage from the gingival margin scored. Statistical analysis was performed using Kruskal Wallis and Mann-Whitney U tests. There was no significant difference between systems in terms of leakage scores when gingival margins were situated in enamel (p=0.70). All restorations with margins in cementum/dentin leaked significantly more than those with margins in enamel (p<0.001). There was no significant difference between leakage scores of 3M Z250 and Filtek P60 with cementum/dentin gingival margins (p=0.68). Use of a flowable composite liner (3M Filtek Flow) against cementum/dentin was associated with increased microleakage (p<0.001). In this study, leakage scores suggest that gingival margins should be placed in enamel. The conventional and packable resin composites tested were not associated with differences in microleakage. Leakage data do not support the use of flowable resin composite linings in Class II resin composite restorations.     

Marginal permeability of self-etch and total-etch adhesive systems

This study evaluated microleakage in vitro of self-etch and multi-step, total-etch adhesive systems. Ninety-six extracted non-carious human molars were randomly assigned to eight groups (n=12) and restored with different adhesive systems: Optibond Solo Plus, iBond, Adper Prompt L-Pop, Xeno III, Simplicity, Nano-Bond, Adper Scotchbond Multi-Purpose and Touch & Bond. Each group was treated following the manufacturer's instructions. Class V cavities were prepared on the facial or lingual surfaces of each tooth with coronal margins in enamel and apical margins in cementum (dentin). The teeth were restored with Z-100 resin composite. After polishing with Sof-Lex disks, the teeth were thermocycled for 1000 cycles and coated with nail varnish to within 1.0 mm of the restoration. The teeth were stained in 1% methylene blue dye for 24 hours and sectioned from the facial to lingual surface. Dye penetration (microleakage) was examined with a 20x binocular microscope. Enamel and dentin margin leakage was scored on a 0 to 3 ordinal scale. Data were analyzed using Kruskal-Wallis Analysis of Variance and Mann-Whitney U tests. Comparison of the adhesive groups at the enamel margin revealed: 1) Adper Scotchbond Multi-Purpose exhibited significantly less leakage than the other adhesive groups (except iBond); 2) among the self-etch adhesive groups, iBond exhibited significantly less leakage than Nano-Bond and 3) the other adhesive groups clustered intermediately. In contrast, there were no significant differences among the adhesive groups when the dentin margin was evaluated. A Wilcoxin signed rank test showed significantly less leakage at the enamel margins compared to the dentin margins of the eight adhesive systems tested. All data were submitted to statistical analysis at p<0.05 level of significance.     

A microleakage study of single-bottle adhesives applied to enamel and cementum and aged by both occlusal loading and thermocycling.

OBJECTIVE: To compare enamel and dentin microleakage of three single-bottle dentin-bonding agents (DBA) (One-Step, Single Bond, and Dentamed P&B) on teeth that were then subjected to thermocycling and occlusal loading. METHOD AND MATERIALS: Class 5 cavities were prepared on the buccal and lingual surfaces of 30 extracted human molars and divided into three groups. The occlusal margins were in enamel, and the cervical margins were in cementum. DBA were applied strictly according to the manufacturers' recommendations, and the preparations were restored with resin composite. Specimens were subjected to 2,000 intermittent occlusal loads and 3,000 thermocycles and then immersed in 0.5% basic fuchsin dye for 72 hours. Four consecutive sections were cut for each specimen and examined under a stereomicroscope. The extent of dye penetration was measured and recorded using a nonparametric scale from 0 to 4. RESULTS: The Mann-Whitney U test showed a significant difference at cementum margins between One-Step and Dentamed P&B, as well as between Single Bond and Dentamed P&B, and an insignificant difference between Single Bond and One-Step. There was no significant difference in dye penetration at enamel margins between the three DBA. CONCLUSION: Under combined use of occlusal loads and thermocycling, the sealing ability of single-bottle DBA at dentin margins ranged from good for One-Step to moderate for Single Bond to poor for Dentamed P&B. At enamel margins, all materials performed equally well.     

Microleakage of Composite Restorations Bonded with Three New Dentin Bonding Agents

ABSTRACT: This report evaluates the impact of two dentin bonding techniques on microleakage of Class V restorations bonded with three new dentin bonding systems. Forty-eight extracted human premolar and molar teeth were randomly assigned to four groups for bonding with EBS Bonding System (ESPE, Seefeld, Germany); Syntac Single-Component (Ivoclar-Vivadent, Schaan, Liechtenstein); Scotchbond 1 (3M Dental Products, St. Paul, Minnesota); and a control, Prime and Bond 2.1 (Dentsply, DeTrey, Konstanz, Germany). Cavities were cut in both the buccal and lingual surfaces. The coronal half of each preparation was in enamel, and the gingival half was in cementum or dentin. The cavities were restored with composite after the application of dentin bonding agents, using two different drying techniques for each material. The teeth were stored in distilled water for 6 days at 37°C and then thermocycled. The restorations were examined microscopically for leakage, using Procion brilliant red as a marker. All groups showed microleakage at both the enamel and dentin margins. At the gingival margin, there was no significant difference between any of the experimental materials and the control for either wet bonding (Kruskal-Wallis: p .8920) or dry bonding (Kruskal-Wallis: p .9973); or between the two techniques for each material (Mann-Whitney U in all cases p > .05). Scanning electron microscopic examination confirmed that the zones of microleakage, as indicated by dye penetration, were principally resin-cohesive failures within the collagen-rich hybrid layer. Three water-based bonding agents were unable to prevent microleakage at either the enamel or dentin margins of Class V cavities regardless of which bonding technique was employed. All systems behaved equally.     

Microleakage of composite restorations bonded with three new dentin bonding agents.

This report evaluates the impact of two dentin bonding techniques on microleakage of Class V restorations bonded with three new dentin bonding systems. Forty-eight extracted human premolar and molar teeth were randomly assigned to four groups for bonding with EBS Bonding System (ESPE, Seefeld, Germany); Syntac Single-Component (Ivoclar-Vivadent, Schaan, Liechtenstein); Scotchbond 1 (3M Dental Products, St. Paul, Minnesota); and a control, Prime and Bond 2.1 (Dentsply, DeTrey, Konstanz, Germany). Cavities were cut in both the buccal and lingual surfaces. The coronal half of each preparation was in enamel, and the gingival half was in cementum or dentin. The cavities were restored with composite after the application of dentin bonding agents, using two different drying techniques for each material. The teeth were stored in distilled water for 6 days at 37 degrees C and then thermocycled. The restorations were examined microscopically for leakage, using Procion brilliant red as a marker. All groups showed microleakage at both the enamel and dentin margins. At the gingival margin, there was no significant difference between any of the experimental materials and the control for either wet bonding (Kruskal-Wallis: p = .8920) or dry bonding (Kruskal-Wallis: p .9973); or between the two techniques for each material (Mann-Whitney U in all cases p > .05). Scanning electron microscopic examination confirmed that the zones of microleakage, as indicated by dye penetration, were principally resincohesive failures within the collagen-rich hybrid layer. Three water-based bonding agents were unable to prevent microleakage at either the enamel or dentin margins of Class V cavities regardless of which bonding technique was employed. All systems behaved equally.     

The effect of different types of flowable restorative resins on microleakage of Class V cavities

This study evaluated the microleakage of Class V cavities restored with three different types of flowable resin restorative material and compared the effects of using their respective manufacturer's dentin adhesive or a different brand. Class V cavities with the occlusal margin in enamel and the gingival margin in dentin were prepared on the buccal and lingual surfaces of 48 non-carious human molars. The teeth were randomly assigned to three equal groups of 16. The first eight teeth in each group were restored with one of the flowable restorative materials (Filtek Flow, Dyract Flow, Admira Flow) using the manufacturer's recommended dentin adhesive (Single Bond, Prime & Bond NT, Admira Bond), and the remaining eight molars were restored using a different brand of dentin adhesive (Gluma Comfort Bond). The samples were thermocycled 200 times (5 degrees C-55 degrees C) with a one-minute dwell time. They were then immersed in a 2% basic fuchsin solution for 24 hours, sectioned and analyzed by stereomicroscopy. There was no statistically significant difference at the occlusal margins for either restoration used with its respective dentin adhesive. At the gingival margins, there was a significant difference among all groups. Flowable ormocer (Admira Flow/Admira Bond) displayed the least leakage at the gingival margins. When these flowable restoratives were used with a different brand of dentin adhesive, statistically significant differences were observed both on enamel and dentin. None of the restoratives tested fully prevented leakage at the gingival margins. No significant differences in microleakage were observed among the restorative materials used with respect to the manufacturer's dentin adhesive or a different brand except for Admira Flow restorative at the gingival margins. The gingival margins had significantly more microleakage than the occlusal margins (p < 0.05) except in the Admira Flow group, where microleakage at the occlusal and gingival margins was almost equal.     

Microleakage of Class V restorations using two different compomer systems: an in vitro study

This study compared the marginal microleakage of Class V cavities restored with Dyract-AP and F2000. Forty Class V cavity preparations were performed on extracted human teeth. As a negative control, twenty teeth were used without Class V preparations. The apical foramina of the teeth were sealed with a layer of varnish and amalgam restorations. Class V cavity preparations with occlusal margins in enamel, and gingival margins in cementum or dentin that measured approximately three millimeters in width (gingival-occlusal) and length (mesial-distal), were prepared on the buccal surface of the teeth. Samples were divided randomly into 2 groups of 15, and restored per manufacturer's instructions using experimental primer/conditioner (PCC, D/C) and Single Bond Adhesive (3M). All restorations were polished with an abrasive finishing kit. After storage in 37 degrees C water for 24 hours, all specimens were thermocycled between 5 degrees C and 55 degrees C for 500 cycles with a 30-second dwell time, followed by immersion in 0.2% basic fucsine for 24 hours. Teeth were then embedded in cold cure acrylic resin, sectioned longitudinally, and the dye penetration at the enamel and cementum margins were scored at 30x magnification. Evaluations were rated from 0 to 3 (0 = no leakage; 1 = dye penetration up to one-half of the preparation depth; 2 = dye penetration more than one-half preparation depth, but less than the axial wall; 3 = dye penetration along the axial wall). Both F-2000 and Dyract-AP indicated no leakage in the enamel margins. Dyract-AP showed no leakage at either the enamel or dentin margins. Fisher's Exact Test revealed that this difference in dentin margins was statistically significant (p < 0.05). Under the given conditions, Dyract-AP and F-2000 demonstrated resistance to microleakage in enamel, and showed Dyract-AP to be more resistant to microleakage in dentin than F-2000.     

The effect of flexural load cycling on the microleakage of cervical resin composites

Although several in vitro studies have attempted to investigate the microleakage of Class V resin composites under loading, the effect of load cycling on marginal seal is still unclear. This may be due to the fact that axial loads were applied to the specimens. This study investigated the effect of flexural loads on marginal sealing of cervical resin composites. One hundred and fifty cervical wedge-shaped cavities were restored with Clearfil Photo Bond, Clearfil Liner Bond 2, Scotchbond Multi-Purpose with 10% maleic acid, Scotchbond Multi-Purpose with 35% phosphoric acid or Mac-Bond 2 according to the manufacturers' instructions. After the restorations were finished, 10 specimens from each group were immersed in 0.5% basic fuchsin solution to examine microleakage. Prior to dye solution immersion, 20 specimens were subjected to flexural load cycling (1 mm labio-lingual or linguo-labial displacement at the incisal edge, 10,000 cycles, 1 cycle/second). The data were analyzed using the Kruskal-Wallis test and the Mann-Whitney U-test (p<0.05). When the flexural loads were not applied, both the incisal and apical margins showed good marginal sealing, regardless of the adhesive system used. Labio-lingual loading significantly deteriorated the marginal integrity at the incisal enamel margins, except for those restored with Clearfil Photo Bond. However, only Clearfil Photo Bond demonstrated a significant increase in microleakage along the apical dentin margins. Linguo-labial loading had no significant effect on the marginal seal.     

Influence of luting agent on the microleakage of all-ceramic crowns

PURPOSE: In this in-vitro study, microleakage of all-ceramic crowns was evaluated at enamel and dentin margins. MATERIALS AND METHODS: Forty maxillary central incisors were randomly divided into 4 groups (n = 10). While buccal and palatal margins were placed on enamel, mesial and distal margins were placed below the cementoenamel junction. In groups 1 to 3, IPS Empress 2 crowns were luted with Variolink 2/Syntac Classic (group 1), Bifix DC/Solobond Plus (group 2) and Calibra/Prime & Bond NT combinations (group 3), respectively. In the control group (group 4), porcelain-fused-to-metal crowns were luted with a zinc-phosphate cement. All specimens were subjected to 5000 thermocycles (at 5 degrees C to 55 degrees C; 30-s dwell time). After immersion in India ink for 48 h at 37 degrees C, the specimens were sectioned both buccolingually and mesiodistally. Each section was evaluated for microleakage under a stereomicroscope at 24X magnification. RESULTS: According to the Krukal-Wallis test, in all groups, there were significant differences in microleakage at the enamel margins (p = 0.001). Nevertheless, the margins finished in dentin showed no significant differences (p = 0.163). According to the Mann-Whitney U-test, statistically significant differences were observed in microleakage between groups 1 and 3 (p = 0.049), groups 1 and 4 (p = 0.001), groups 2 and 4 (p = 0.002), and between groups 3 and 4 (p = 0.045) at the enamel margin. In group 1, significantly greater microleakage was observed at the dentin margin compared to the enamel margin (p = 0.007). CONCLUSION: The adhesive luting technique demonstrated an excellent ability to minimize microleakage of all-ceramic crowns at the enamel margins. Water-based dentin bonding systems showed less microleakage than the water-free acetone-based dentin bonding system at the enamel margin.     

Effects of potassium oxalate on knoop hardness of etch-and-rinse adhesives

The objective of this study was to determine whether the hardness of etch-and-rinse adhesives may be affected by the pretreatment of acid-etched dentin with potassium oxalate desensitizer. Unerupted human third molars were cut into crown segments by removing the occlusal enamel and roots. The pulp chamber of these crown segments was connected to a syringe barrel filled with phosphate-buffered saline so that the moisture of dentin was maintained during the bonding procedures. Three etch-and-rinse adhesives-two two-step systems (Adper Single Bond 2 [SB], One-Step [OS]) and one three-step system (Adper Scotchbond Multi-Purpose [MP])-were applied to acid-etched dentin that had been treated (experimental groups) or not (control groups) with potassium oxalate (BisBlock). The Knoop hardness (KHN) of adhesives was taken at different sites of the outer surface of the adhesive-bonded dentin. The KHN of the three tested adhesives applied to acid-etched dentin treated with potassium oxalate was significantly lower than that exhibited by the respective controls (not treated with oxalate; p<0.05). Regardless of the adhesive, the treatment with potassium oxalate reduced the adhesives' KHN (p<0.05), with the OS system exhibiting the lowest KHN compared with the MP and SB systems.     

Microleakage of direct and indirect composite restorations with three dentin bonding agents

This study evaluated the marginal sealing ability of direct and indirect (inlay) resin composite restorations with three dentin bonding systems. Forty-eight freshly extracted bovine incisor teeth were randomly assigned to four groups for bonding with Syntac Single-Component, Excite (Vivadent, Liechtenstein), ScotchBond Multi-Purpose Plus (3M Dental Products, St Paul, MN 55144, USA) and a control with no bonding agents. Class V cavities were cut in both buccal and lingual surfaces. The coronal half of each preparation was in enamel and the gingival half was in cementum or dentin. Half of the specimens in each group were restored with direct and the remainder with indirect technique. The teeth were stored in 37 degrees C water for 30 days, then thermocycled. After immersion in 0.5% basic fuchsin, the teeth were cut faciolingually and evaluated for dye penetration using a binocular stereomicroscope. There was no significant difference among the bonding systems for either the direct or indirect technique or between the two techniques used for each system, however, the indirect technique showed significantly (p = 0.001) less microleakage than the direct technique in control groups. All groups showed more leakage at the cementum margins except Excite with direct technique, where microleakage at the incisal and gingival margins was almost equal.     

An in vitro comparison of marginal microleakage of alternative restorative treatment and conventional glass ionomer restorations in extracted permanent molars

PURPOSE: The objective of this study was to compare the marginal leakage of cervical restorations made using alternative restorative treatment (ART) and conventional glass ionomer restorations. METHODS: Sixteen permanent maxillary and mandibular first and second molars extracted for periodontal reasons with Class V carious dentin on the buccal surfaces were prepared using ART while a second set of 29 noncarious molars had Class V preparations made with a high-speed handpiece. The occlusal margin was located in the enamel, and the gingival margin was located in the dentin/cementum. All teeth were restored with glass ionomer cement (GIC). The teeth were thermally stressed for 300 cycles and stained with methylene blue. Samples were sectioned and evaluated for microleakage. RESULTS: One-way analysis of variance on ranks revealed no significant difference in leakage at both the dentin and enamel margins between the conventional and ART groups. The microleakage at the dentin margin, however, was significantly greater (P < .001) than at the enamel margins in the conventional group. CONCLUSION: Alternative restorative treatment with GIC provides enamel and dentin margins that show comparable marginal leakage to conventionally restored permanent teeth. For the conventional restorations, leakage at the dentin margins occurs to a significantly higher extent than at the enamel margins.     

Microleakage at the composite-repair interface: effect of different adhesive systems

The purpose of the present study was to evaluate the microleakage at the composite-repair interface using different bonding systems. Composite resin specimens (Filtek Z250 - 3M-ESPE) were divided into five groups (n=20) according to the following bonding mechanism: C - control - etching with 35% phosphoric acid; SB1 - etching and application of one coat of Single Bond (3M-ESPE); SB2 - etching and application of two coats of Single Bond (3M-ESPE); SMP1 - etching, application of Scotchbond Multi-Purpose primer (3M-ESPE) followed by the adhesive and, SMP2 - etching, application of Scotchbond Multi-Purpose adhesive (3M-ESPE) without the primer. Thereafter, all groups received new resin application. Samples were thermocycled (500 cycles / 5oC - 55oC [±2]) and immersed for 4h in 2% methylene blue buffered dye solution (7.0 pH). Three examiners measured the extent of microleakage in a stereoscope microscope, using four representative scores. For all experimental groups, no significant difference in microleakage at the repair was identified by Kruskal-Wallis test (p > 0.05). Therefore, different types of bonding systems presented the same effect on the dye penetration along the repair interface.     

Effect of dentin deproteinization on microleakage of Class V composite restorations

The role of the collagen fibers in dentin adhesion has not clearly been established. Therefore, this laboratory study evaluated the microleakage at resin-dentin and resin-enamel interfaces of Class V composite restorations after etching enamel and dentin with phosphoric acid (H3PO4) or after etching with H3PO4 followed by deproteinization with 5% sodium hypochlorite (NaOCl) to prevent the formation of a hybrid layer. Ten extracted human molars were used to prepare standardized Class V cavities on both buccal and lingual surfaces. The teeth were randomly divided in two groups: 1) Class V cavities that were etched with H3PO4 for 15 seconds; b) Class V cavities that were etched with H3PO4 for 15 seconds followed by collagen removal with 5% NaOCl for two minutes. The cavities were restored using the Prime & Bond 2.1 bonding system and TPH resin composite. The specimens were stored in water for 24 hours at 37 degrees C and thermocycled 500 times between water baths kept at 5 degrees C and 55 degrees C. After thermocycling, specimens were immersed in a 0.5% aqueous solution of basic fuchsin for 24 hours. Three longitudinal sections of each restoration were obtained and examined with a stereomicroscope for qualitative evaluation of microleakage. The data were statistically analyzed by Mann-Whitney U and Wilcoxon matched pairs signed ranked tests. Extra specimens were analyzed with the scanning electron microscope (SEM). Occlusal margins (enamel margins) resulted in statistical lower degree of leakage than gingival margins (dentin/cementum margins) in both treatment groups. For each type of margin, there were no statistically significant differences between the etched and the etched and deproteinized groups. Under the SEM, occlusal surfaces showed no detachment between enamel and dentin, while dentin/cementum resulted in gap formation.     

Effect of insertion technique and adhesive system on microleakage of Class V resin composite restorations

PURPOSE: To evaluate the effect of different insertion techniques and adhesive systems on microleakage of Class V composite resin restorations. MATERIALS AND METHODS: Forty-eight human molars were randomly assigned to four groups (n = 12). Standardized mixed Class V cavities (enamel and dentin margins) were prepared at the CEJ. A total-etch (Adper Scotchbond MultiPurpose) and a self-etching (iBond) adhesive system were evaluated using either an incremental or single-step (bulk) insertion technique. The preparations were restored with Esthet*X micromatrix hybrid composite. The teeth were thermocycled 1000 cycles, immersed in 1% methylene blue dye for 24 h, and invested in acrylic resin. The specimen blocks were then sectioned longitudinally, with dye penetration (microleakage) examined with a 20X binocular microscope. Enamel and dentin margins were scored separately for microleakage using an ordinal ranking system. Results were analyzed using non-parametric tests at a p < 0.05 level of significance. RESULTS: Significance was exhibited between the groups (adhesive material/insertion technique) at the coronal and apical margins. At the coronal margin, the total-etch adhesive/incremental insertion group exhibited significantly less leakage than the other groups, while at the apical margin, the total-etch adhesive/incremental insertion group showed significantly less leakage than the self-etching adhesive/bulk insertion group. Significantly less leakage was found at the coronal margins compared to the apical margins of the material/technique groups. CONCLUSION: The use of a total-etch adhesive system and incremental insertion of composite significantly reduced microleakage at the coronal and apical margins of Class V composite restorations.     

The role of cavity preparation and conditioning in the leakage of restorations

PURPOSE: Restoration microleakage is thought to be determined by the method and location of cavity preparation, enamel etching, and dentin conditioning, as well as the restorative material. This study compared the microleakage of composite restorations placed in preparations cut with carbide and diamond burs and those treated with different bonding/conditioning agents. MATERIALS AND METHODS: Class V preparations (3 x 2 x 2 mm) were cut wholly in enamel or in enamel and cementum in 100 human premolars. Twenty teeth were prepared with carbide burs and the preparations etched and conditioned with Prime&Bond NT but not restored. A second set of 20 teeth had enamel-only preparations cut with carbides (n = 10) or diamonds (n = 10), and the preparations etched, conditioned (Prime&Bond NT) and restored with Prisma TPH. The other 60 teeth were divided into 3 groups of 20 teeth each with enamel-only (n = 10) or enamel/cementum preparations (n = 10). The 3 groups of teeth were conditioned with Optibond Solo, Clearfil SE Bond or Prompt-L-Pop prior to restoration with Prisma TPH. Two mm of root was resected from all teeth, pulpal tissue removed, and insulated copper wires inserted via the root canals to contact with the pulp chamber roof before the tooth-wire interfaces and root surfaces were sealed. The teeth were immersed in 0.9% NaCl and leakage assessed over 30 d by iR drop across a resistor in series with a DC source and stainless-steel counter electrode. RESULTS: Differences (p < 0.05) in leakage were found for enamel preparations cut with carbides and diamonds, and the relationship of leakage vs time was linear. Enamel/cementum preparations showed greater leakage, and the relationship of leakage vs time was sigmoidal. Conditioned-only preparations showed the same leakage as those conditioned and restored, while preparation leakage varied with the conditioning agent. CONCLUSION: Cavity preparation location, method of cutting, and the conditioning agent markedly affect leakage behavior.     

Microleakage of seven adhesive systems in enamel and dentin

AIM: The aim of this study was to evaluate the microleakage of seven adhesive systems on two substrates (enamel and dentin). METHODS AND MATERIALS: Class V cavities were performed in buccal and lingual surfaces of 56 bovine incisors. The cervical margin was located in dentin and the incisal margin in enamel. The specimens were randomly divided into seven groups (n=16), according to the adhesive system employed: Single Bond; Excite; One Step Plus; Gluma One Bond; Magic Bond; One Up Bond F; and One Coat Bond. The cavities were incrementally filled with a hybrid composite Filtek Z250 and polymerized with a XL 3000 light curing unit. After polishing, the specimens were submitted to thermal cycling followed by dye immersion. Leakage was evaluated under magnification (40X) based on a standard ranking. Data were subjected to statistical analysis (Kruskal-Wallis). RESULTS: Enamel margins exhibited lower leakage than dentin margins (p<0.01). The majority of the specimens were leakage-free and materials performed similarly. Conversely, in dentin most of the specimens exhibited the highest leakage degree and significant differences among materials (p<0.05) were found, with Excite exhibiting the lowest leakage degree. It was concluded enamel provided better sealing and the adhesive system was a significant factor only in dentin.     

Technical note: fluoride pre-treatment effects on microleakage of a resin bonding agent

This pilot study reports the effect of fluoride pre-treatment of dentin and enamel on microleakage of a resin bonding agent. Pairs of circular class V cavities involving 50% enamel and 50% cementum were cut in 12 extracted third molars. Each tooth contained a control and a treatment cavity (matched-pair design). A stannous fluoride solution (0.717% SnF2, Gel-Kam, Scherer Laboratories, Dallas, TX) was applied to the entire treatment preparation of each tooth. A dentin resin bonding system (Scotchbond 2 with Scotchprep, a dentin primer, 3M Dental Products Div., St. Paul, MN) was applied per manufacturer's instructions. Silux resin (3M) was placed over the resin bonding agent, light-cured, and finished. The teeth were subjected to 500 temperature cycles between 5 and 55 degrees C. A silver nitrate staining technique was used for evaluation of microleakage. The mean leakage for the control was 2235 +/- 1146 microns, and 2105 +/- 1052 microns for the fluoride treatment sites. It is concluded, from this pilot study with small sample size, that pre-treatment with fluoride of dentin and enamel prior to the placement of a resin bonding agent produced no significant change in microleakage (Student's t test for paired samples, p = 0.6492).