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1.
膜内成骨是骨骼形成的重要方式,其受多条信号通路的调控,Notch信号通路是其中重要的一条。异常的Notch信号通路影响骨骼的形成。深入探讨Notch信号通路在膜内成骨中的作用机制,有利于揭示临床多种骨骼畸形,尤其是颅颌面骨发育异常的发生机制,提供新的治疗靶标。本文概述Notch信号通路对膜内成骨过程的调控。 相似文献
2.
目的 检测rhBMP-2/hTGF-β1 /胶原复合材料与rhBMP-2/hTGF-β1生物学活性的差别。方法 本研究于2010年5—12月在福建医科大学附属口腔医院实验室进行。分离培养的Beagle犬骨髓基质细胞中加入质量浓度相同的rhBMP-2/hTGF-β1或rhBMP-2/hTGF-β1 /胶原复合材料后继续培养4 d,通过细胞计数(MTT法)、碱性磷酸酶(ALP)活性检测,分别对rhBMP-2/hTGF-β1 /胶原复合材料和rhBMP-2/hTGF-β1的生物学活性进行检测,并比较其活性差别。结果 rhBMP-2/hTGF-β1/胶原复合材料和rhBMP-2/hTGF-β1均具有生物学活性,并且其生物学活性差异无统计学意义(P > 0.05)。结论 这种合成rhBMP-2/hTGF-β1 /胶原复合材料的方法不会改变生长因子的生物学活性。 相似文献
3.
目的探讨核转录因子一KB(NF—KB)/抑制因子KB(IKB)信号通路在调控牙龈卟啉单胞菌(Pgingivalis)感染诱导血管内皮细胞细胞间黏附分子-1(ICAM-1)高表达过程中的作用。方法本研究于2008年3月至2011年1月在中国医科大学中心实验室及中国医科大学口腔医学院中心实验室进行。已知P.gingivalis感染血管内皮细胞可诱导其ICAM-1基因和蛋白表达增高。采用Real-timePCR和Westernblot法检测P.gingivalis感染后血管内皮细胞ICAM-1mRNA和蛋白表达;应用5、10、20μmol/L的蛋白酶体抑制剂MGl32分别预处理血管内皮细胞15、30、60min,再将细胞与P.gingivalis共同培养8h,观察细胞ICAM-1mRNA和蛋白表达的变化,分析NF—KB/IKB信号通路对ICAM-1表达的调控作用。结果10txmol/LMGl32预处理30rain,Pgirtgivalis感染的血管内皮细胞ICAM—lmRNA表达具有下降趋势;20p~mol/LMGl32预处理15min,P.gingivalis感染的血管内皮细胞ICAM-1表达即开始下降,预处理时间延长至30、60min,ICAM-1表达下降更趋明显。结论阻断NF—KB/IKB信号通路可抑制P.gingivalis感染诱导的血管内皮细胞ICAM-1表达。 相似文献
4.
目的:探究七氟烷对人舌鳞状细胞癌Tca-8113细胞的凋亡机制.方法:用2~10 μmol/L七氟烷作用体外培养人舌鳞状细胞癌Tca-8113细胞.MTT实验检测细胞活力;Hochest/PI双染法以及流式细胞术检测细胞凋亡;Western blot法检测MAPK/STAT3信号通路中相关蛋白表达含量的变化以及通过加入... 相似文献
5.
目的:探讨热放疗对舌鳞癌Tca 8113细胞和耐药的Tca 8113/CBDEA细胞耐药性的影响。方法:实时定量逆转录PCR(RT—PCR)检测热放疗对Tca 8113细胞和耐药的Tca 8113/CBDEA细胞多药耐药基因1(MDR1)、多药耐药相关蛋白1基因(MRP1)和谷胱甘肽硫-转移酶π基因(GST-π)表达的影响和检测热放疗对细胞内阿霉素浓度的影响。结果:Tca 8113/CBDEA和Tca 8113细胞热放疗后耐药基因MDR1的表达在4h和24h无明显改变(P〉0.05)。MRP1基因的表达在4h和24h组均有明显下降(P〈0.05),GST-π基因表达在4h组Tca 8113和Tca 8113/CBDEA的表达无明显改变(P〉0.05),在24h组有明显下降(P〈0.05).热放疗以后肿瘤细胞内阿霉素(ADM)浓度有明显上升。结论:热放疗联合应用抑制了放疗造成的耐药基因表达上升,增加了细胞内的药物浓度,热放疗联用不会促使肿瘤细胞产生MDR。 相似文献
6.
目的 探讨环加氧酶(COX) -2抑制剂尼美舒利(NIM)对人舌鳞状细胞癌Tca8113细胞中ang-2基冈表达的影响及其意义.方法 以25、50、100和200 μmol·L-1浓度的NIM处理Tca8113细胞48 h,半定量RTPCR检测其ang-2 mRNA的表达.结果 Tca8113细胞中ang-2 mRNA... 相似文献
7.
目的:探讨三苯氧胺(Tamoxifen,TAM)对人舌鳞癌细胞系Tca8113的增殖抑制作用。方法:不同浓度TAM处理体外培养的人舌鳞癌细胞Tca8113,MTT法检测细胞增殖,倒置显微镜下观察细胞形态学变化。结果:在浓度为1、2、5、10μmol/L时,TAM呈现出剂量依赖性抑制人舌鳞癌细胞Tea8113的增殖作用。通过对三个不同浓度(2μmol/L、5μmol/L、10μmol/L)TAM实验组的抑制率进行比较,差异有统计学意义(P〈0.05),细胞抑制率与药物浓度呈正相关。细胞超微结构显示,药物浓度与细胞结构呈剂量依赖关系。结论:TAM可显著抑制人舌鳞癌细胞Tea8113的增殖,其作用呈剂量依赖关系。 相似文献
8.
Wei Zhong Li Xiao Yan Wang Zu Guo Li Jin Hua Zhang Yan Qing Ding 《Journal of oral pathology & medicine》2010,39(7):579-584
J Oral Pathol Med (2010) 39 : 579–584 Background: Overexpression of cyclooxygenase‐2 (COX‐2) is associated with carcinogenesis, invasiveness, and metastasis of malignant tumors. Inhibition of COX‐2 is one hot topic of research in prevention and treatment of malignant tumors. Because of the selective and specific inhibition on the activity of COX‐2, the roles of celecoxib in prevention and treatment of tumors have attracted broad attention in recent years. In this study, we investigated the inhibitory effect of celecoxib combined with cisplatin on the proliferation of human tongue squamous cell carcinoma cell line Tca8113 in vivo and in vitro. Methods: Human tongue squamous cell carcinoma tumor cells Tca8113 and a mouse model with Tca8113 cells were used to study the growth inhibition of cisplatin enhanced by celecoxib. Drug treatment of Tca8113 in vitro and mice bearing xenografts in vivo were used. The level of COX‐2 expression was detected by Western blotting. Sensitivity of cells to drug treatment was analyzed by MTT assay. Results: Treatment of Tca8113 cells with cisplatin (CDDP) had less effect on the expression of COX‐2, whereas the COX‐2 expression was significantly down‐regulated after treatment with celecoxib alone or in combination with CDDP for 24 h. In addition, the combination of celecoxib with CDDP was also able to inhibit the Tca8113 line heterotransplanted in Balb/c nude mice. Conclusions: Those findings indicate that a low dose of celecoxib could augment CDDP‐induced growth inhibition of Tca8113 cells and its xenograft in Balb/c nude mice. 相似文献
9.
目的探讨α亚族趋化因子受体3(CXC-chemokine receptor 3,CXCR3)在不同人舌鳞癌细胞株的表达以及其配体干扰素诱导蛋白-10(interferon induced protein 10,IP-10)对人舌鳞癌细胞株CAL-27增殖和凋亡的影响。方法采用蛋白印迹法和免疫荧光染色对3种人舌鳞癌细胞株(CAL-27、UM-1、Tca-8113)IP-10的相应受体CXCR3的表达进行检测。CAL-27细胞被分为3个实验组和1个对照组,3个实验组根据IP-10的刺激浓度,分为10 ng/mL组、20 ng/mL组、40 ng/mL组,对照组不予刺激。12 h、24 h、48 h时检测4组细胞的增殖;24 h时用流式细胞仪检测细胞的凋亡。结果 CXCR3在3种人舌鳞癌细胞株均有表达,并且CXCR3在3株细胞的胞膜及胞质内均有染色。和对照组相比,12 h、24 h时,3种浓度的IP-10均能够促进CAL-27细胞增殖(P<0.05);在48 h时,只有40 ng/mL的IP-10能够促进CAL-27细胞增殖(P<0.05),10 ng/mL、20 ng/mL的IP-10对CAL-27细胞的增殖无促进作用(P>0.05)。在24 h时,对照组、10 ng/mL组、20 ng/mL组和40 ng/mL组的细胞凋亡率分别为(0.053 3±0.472 6)%、(3.236 7±0.940 0)%、(4.516 7±1.115 4)%和(3.363 3±0.571 2)%,3种浓度IP-10均能够促进CAL-27细胞的凋亡(P<0.05),但3个实验组之间差异无统计学意义(P>0.05)。结论CXCR3在3种人舌鳞癌细胞株的胞膜及胞质内均有表达;IP-10既能促进CAL-27细胞的增殖,又能促进其凋亡。随着时间的延长,IP-10的促增殖作用减弱,而这种减弱可能是由IP-10的促凋亡作用的逐步发挥所引起的。 相似文献
10.
目的应用抑制消减杂交(SSH)技术筛选舌鳞癌干细胞相关差异表达基因。方法以舌鳞癌Tca8113细胞株中的醛脱氢酶(ALDH)高表达(ALDHbr)细胞与低表达(ALDHlow)细胞互为实验组和对照组,进行正反向消减杂交后建立差异表达基因文库,随机挑选差异基因克隆进行测序及生物信息学分析。结果经Blast比对分析,得到62条差异表达基因;功能聚类发现多数与细胞周期调节、细胞分化等生物行为相关;信号通路分析显示有11条基因参与的12条信号通路发生显著改变。结论应用SSH技术成功筛选出了与舌鳞癌干细胞相关的一些基因,进一步研究这些基因对探索舌鳞癌干细胞特异性表面标志物具有重要意义。 相似文献
11.
EMMPRIN expression in tongue squamous cell carcinoma 总被引:1,自引:0,他引:1
Zhiquan Huang Hongzhang Huang Haigang Li Weiliang Chen Chaobin Pan 《Journal of oral pathology & medicine》2009,38(6):518-523
Background: Extracellular matrix metalloproteinase inducer (EMMPRIN) is identified as a tumor-cell membrane protein that stimulates matrix metalloproteinases (MMPs) production. Several studies have shown that higher EMMPRIN expression is associated with shorter survival time and correlated significantly with more advanced clinico-parameters of cancer. The aim of this study was to investigate the relationship between clinico-pathologic characteristics and EMMPRIN, and prognostic significance of EMMPRIN expression in human tongue squamous cell carcinoma.
Methods: Extracellular MMP inducer expression was examined immunohistochemically on paraffin-embedded tissue specimens from 68 patients with tongue squamous cell carcinoma and who underwent radical surgeries from 1996 to 2006. The 68 patients were followed up from 1 to 119 months, with an average of 27.5 months. Nonparametric tests were performed for the comparison of EMMPRIN expression between two independent groups. Survival analysis was performed to find the prognostic significance of EMMPRIN expression.
Results: We found that EMMPRIN expression in tongue squamous cell carcinoma is significantly higher than that in non-cancerous epithelium adjacent to carcinoma of tongue. In addition, EMMPRIN expression is significantly associated with tumor diameter and clinical stage in the samples, but did not correlate with gender, age, tumor metastasis, and pathological grade. Finally, survival analysis indicates that EMMPRIN overexpression correlates significantly with poor overall survival in the patient cohort.
Conclusion: These results suggest that EMMPRIN might represent an attractive target for immunotherapeutic approaches in a subgroup of patients with tongue squamous cell carcinoma. 相似文献
Methods: Extracellular MMP inducer expression was examined immunohistochemically on paraffin-embedded tissue specimens from 68 patients with tongue squamous cell carcinoma and who underwent radical surgeries from 1996 to 2006. The 68 patients were followed up from 1 to 119 months, with an average of 27.5 months. Nonparametric tests were performed for the comparison of EMMPRIN expression between two independent groups. Survival analysis was performed to find the prognostic significance of EMMPRIN expression.
Results: We found that EMMPRIN expression in tongue squamous cell carcinoma is significantly higher than that in non-cancerous epithelium adjacent to carcinoma of tongue. In addition, EMMPRIN expression is significantly associated with tumor diameter and clinical stage in the samples, but did not correlate with gender, age, tumor metastasis, and pathological grade. Finally, survival analysis indicates that EMMPRIN overexpression correlates significantly with poor overall survival in the patient cohort.
Conclusion: These results suggest that EMMPRIN might represent an attractive target for immunotherapeutic approaches in a subgroup of patients with tongue squamous cell carcinoma. 相似文献
12.
舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)是口腔恶性肿瘤中最具侵袭性的一种,占口腔癌的50%~60%。尽管在诊断和治疗技术上有了相当大的进步,但是TSCC仍然是头颈部最致命的癌症类型之一。MicroRNAs是内源性合成的小的非编码RNA,负责mRNA表达的转录后调控。它们作为有效的致癌基因或肿瘤抑制因子参与调控了包括TSCC在内的各种疾病的发病机制及过程。在TSCC样本中进行大量的MicroRNA分析,揭示了它们对TSCC起始、发展、转移、化疗耐药、复发和预后的潜在分子机制的作用。本文就MicroRNA与TSCC的相关性研究作一综述总结。 相似文献
13.
随着免疫学技术和分子生物学的发展,生物治疗尤其是主动免疫治疗为舌鳞状细胞癌的预防和治疗提供了新的思路。本文从免疫细胞、肿瘤抗原和细胞因子等方面对近年来舌鳞状细胞癌免疫治疗的研究进展作一综述。 相似文献