腹腔镜胃十二指肠溃疡穿孔修补36例临床观察

目的:观察腹腔镜胃十二指肠溃疡穿孔修补的效果。方法:分析74例胃十二指肠溃疡穿孔修补术患者的临床资料,其中行腹腔镜胃十二指肠穿孔修补术(腔镜组)36例,开腹手术穿孔修补术(开腹组)38例。观察2组患者的手术时间、出血量及术后胃肠道恢复时间、疼痛程度、并发症发生率、住院时间、住院费用等。结果:所有患者手术均顺利完成,腔镜组无中转开腹。2组患者在手术时间、术中出血量、术后疼痛评分、胃肠功能恢复时间、住院时间及住院费用差异均有统计学意义(P<0.01),术后并发症发生率差异无统计学意义(P>0.05)。结论:腹腔镜溃疡穿孔修补术具有创伤小、术后恢复快和并发症发生率低等优点,是治疗胃十二指肠溃疡穿孔的一种安全有效的术式,值得临床推广。     

开腹与腹腔镜上消化道溃疡穿孔修补术疗效比较

目的对比开腹与腹腔镜上消化道溃疡穿孔修补术的临床效果。方法选取洛阳东方医院2013年5月至2015年5月收治的70例消化道溃疡穿孔患者,随机分为观察组和对照组,各35例。观察组通过腹腔镜行上消化道溃疡穿孔修补术治疗,对照组行开腹手术治疗,对比两组临床效果。结果观察组手术时间、术后首次排气时间短于对照组(P<0.05),术中出血量、术后1~4 d的引流量少于对照组(P<0.05),并发症发生率、术后VAS疼痛评分低于对照组(P<0.05)。结论消化道溃疡穿孔经腹腔镜上消化道溃疡穿孔修补术治疗,操作简单且不会产生较大的创伤,利于患者恢复。     

腹腔镜胃十二指肠溃疡穿孔修补术32例疗效分析

目的探讨对急诊科收治的胃十二指肠溃疡穿孔患者实施腹腔镜手术的疗效。方法 62例胃十二指肠溃疡穿孔修补术患者根据术式不同分为A组32例(行腹腔镜手术),B组30例(行开腹手术),比较A组和B组的手术时间、术中出血量、胃肠功能恢复时间、疼痛程度评分、住院时间及并发症情况。结果与B组比较,A组手术时间短,术中出血量少,术后胃肠功能恢复快,且患者疼痛程度轻,住院时间短,差异有显著性(P<0.05)。A组术后并发症发生率达3.1%;B组术后并发症发生率达16.7%,差异有显著性(P<0.05)。结论腹腔镜胃十二指肠溃疡穿孔修补术具有创伤小、术后恢复快、住院时间短、并发症少等优点,值得广泛应用于急诊手术。     

腹腔镜下穿孔修补术对老年胃溃疡合并胃穿孔患者的疗效

目的探讨腹腔镜下穿孔修补术对老年胃溃疡合并胃穿孔患者的疗效。方法选取2017年10月至2018年10月舞阳县人民医院收治的84例老年胃溃疡合并胃穿孔患者,根据手术方法分为常规组和观察组,每组42例。常规组患者接受开腹穿孔修补术治疗,观察组患者接受腹腔镜下穿孔修补术治疗。比较两组手术相关指标、胃肠动力恢复指标、术后并发症发生率。结果观察组术后住院天数短于对照组,术中出血量少于常规组,差异有统计学意义(均P<0.05)。两组手术时间比较,差异无统计学意义(P>0.05)。观察组术后24、48 h GAS水平高于常规组,肠鸣音恢复时间和肛门排气时间均短于常规组,差异有统计学意义(均P<0.05)。观察组术后并发症发生率[2.38%(1/42)]低于常规组[21.43%(9/42)],差异有统计学意义(P<0.05)。结论采用腹腔镜下穿孔修补术治疗胃溃疡合并胃穿孔的术中出血量少,患者术后恢复快,并发症少,有利于胃肠功能恢复。     

腹腔镜胃十二指肠溃疡穿孔修补术与开腹手术治疗胃十二指肠溃疡穿孔的疗效比较

目的对腹腔镜胃十二指肠溃疡穿孔修补术的疗效进行探讨和分析,并与开腹穿孔修补术进行对比分析。方法选取2015年1月～2018年1月在我院住院治疗的胃十二指肠溃疡穿孔患者共70例,将其中35例行腹腔镜胃十二指肠穿孔修补术的患者设为观察组,另外行开腹胃十二指肠穿孔修补术的患者35例设为对照组,对两组患者的手术各项观察指标及术后并发症情况进行对比分析。结果观察组患者的手术时间、胃肠功能恢复时间及住院时间显著短于对照组,观察组患者的出血量(8.23±1.42)m L,明显少于对照组,差异具有显著性(P0.05)。观察组患者的术后并发症发生率明显低于对照组,差异具有显著性(P0.05)。结论腹腔镜胃十二指肠溃疡穿孔修补术较开腹手术创伤小、出血少、术后并发症少、安全性好、患者术后恢复快,值得临床推广和应用。     

腹腔镜与开腹胃十二指肠溃疡穿孔修补术临床效果对比及对患者WBC、CRP和PCT影响

目的：探讨腹腔镜与开腹胃十二指肠溃疡穿孔修补术临床效果对比及对患者WBC、CRP和PCT的影响。方法：选自我院于2015年5月~2016年12月期间收治的胃十二指肠溃疡急性穿孔行穿孔修补手术患者60例,按照随机数字表法分为腹腔镜组30例与开腹组30例。腹腔镜组采用腹腔镜胃十二指肠溃疡穿孔修补术治疗,开腹组采用开腹胃十二指肠溃疡穿孔修补术治疗。比较两组手术时间、术中出血量、切口长度、住院时间、胃肠功能恢复时间、自主下床活动时间、术后并发症及术前和术后WBC、CPR、PCT变化。结果：腹腔镜组手术时间短于开腹组,术中出血量少于开腹组,切口长度小于开腹组,住院时间短于开腹组,均有统计学差异(P<0.05);腹腔镜组胃肠功能恢复时间和自主下床活动时间优于开腹组,且术后并发症少于开腹组,均有统计学差异(P<0.05);两组术后WBC、CRP及PCT均增加,但腹腔镜术后明显低于开腹组,均有统计学差异(P<0.05)。结论：腹腔镜胃十二指肠溃疡穿孔修补术临床效果优于开腹组,且对WBC、CRP和PCT的影响小,具有重要研究意义。     

不同急诊手术方式治疗老年急性上消化道溃疡穿孔修补术的疗效比较

目的比较不同急诊手术方式治疗老年急性上消化道溃疡穿孔的疗效。方法回顾性分析2016年1月—2018年12月秦皇岛市第一医院收治的老年急性上消化道溃疡穿孔患者94例临床资料,根据手术方式的不同分为2组,开腹组(41例)采取开腹穿孔修补术,腹腔镜组(53例)采取腹腔镜下穿孔修补术。比较2组患者临床疗效、围手术期指标(手术时间、术中出血量、术后胃肠功能恢复时间、术后排气时间、住院天数、术后并发症)及血清炎性因子(hs-CRP、TNF-α、IL-6)含量的差异。结果腹腔镜组治疗总有效率为96.23%,高于开腹组的78.05%(χ~2=5.738,P<0.05)。与开腹组比较,腹腔镜组手术时间和术中出血量明显较少,术后胃肠功能恢复时间、排气时间和住院天数明显缩短(t/P=7.848/<0.001,9.953/<0.001,10.797/<0.001,4.598/<0.001,9.596/<0.001),腹腔镜组术后并发症总发生率较开腹组显著降低(χ~2=7.069,P<0.01)。腹腔镜组术后血清hs-CRP、TNF-α和IL-6含量较开腹组明显下降(t/P=5.203/<0.001,6.683/<0.001,4.424/<0.001)。结论腹腔镜下穿孔修补术可有效改善老年急性上消化道溃疡穿孔患者的临床疗效,具有较高的有效性和安全性,值得临床推广应用。     

传统开腹与腹腔镜穿孔修补术治疗胃十二指肠溃疡穿孔临床疗效对比分析

目的:分析对比传统开腹与腹腔镜穿孔修补术治疗胃十二指肠溃疡穿孔临床疗效。方法:选取84例胃十二指肠溃疡穿孔患者分为观察组和对照组,分别给予传统开腹修补和腹腔镜穿孔修补,比较两组患者手术情况,术后恢复情况、住院时间/费用及并发症发生率。结果:两组患者住院费用和溃疡愈合率,差异无统计学意义(P>0.05),观察组患者手术切口、手术时间、术中出血量、术后肛门排气时间、胃肠功能恢复时间、术后下床活动时间及并发症发生率显著低于对照组,差异均有统计学意义(P<0.05)。结论:腹腔镜穿孔修补术治疗胃十二指肠溃疡穿孔创伤小、出血量少、缩短患者术后恢复时间,降低并发症的发病率,溃疡与愈合率与开腹手术无异,值得进一步推广应用。     

腹腔镜胃十二指肠溃疡穿孔修补术36例临床疗效研究

目的探讨腹腔镜胃十二指肠溃疡穿孔修补术的临床效果。方法将我院2009年3月～2012年1月行腹腔镜胃十二指肠溃疡穿孔修补术36例作为腹腔镜组进行研究,另选择同期行开腹胃十二指肠溃疡穿孔修补术治疗的32例患者作为对照组,对比两组患者的手术时间、术中出血量、住院时间、术后并发症情况。结果腹腔镜组手术时间短、术中出血量少、术后住院时间短、术后出现切口感染、肺部感染、腹腔脓肿、粘连性肠梗阻等并发症明显少于对照组,两组上述手术观察指标比较,差异有统计学意义(P<0.05)。术后随访6～12个月,两组均未出现溃疡复发症状,胃镜提示溃疡已愈合。结论腹腔镜胃十二指肠穿孔修补术手术效果效可靠,疗效优于开腹胃十二指肠溃疡穿孔修补术,具有术后恢复快、并发症少等优点,易于被患者接受,值得推广和应用。     

腹腔镜与开腹胃十二指肠溃疡穿孔修补术的疗效比较

目的观察腹腔镜与开腹胃十二指肠溃疡穿孔修补术的临床效果,探讨腹腔镜胃十二指肠溃疡穿孔修补术的临床价值及优越性。方法回顾性分析2008年11月-2012年11月郴州市桂阳县人民医院收治的92例胃十二指肠溃疡穿孔患者的临床资料,其中采用腹腔镜胃十二指肠溃疡穿孔修补术者61例(腹腔镜组),采用开腹胃十二指肠溃疡穿孔修补术者31例(开腹组),观察两组患者手术情况、术后恢复情况及并发症情况。结果腹腔镜组手术时间、术中出血量、术后排气时间及住院时间均明显低于开腹组(P<0.05);腹腔镜组术后需使用镇痛药物患者的比例明显低于开腹组(P<0.05);腹腔镜组术后并发症发生率明显低于开腹组(P<0.05)。结论腹腔镜胃十二指肠溃疡穿孔修补术具有创伤小、恢复快、并发症少等优点,是一种较佳的微创手术方案,值得临床推广应用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.