干扰素γ/Fas介导的HaCaT细胞凋亡及8种人参皂甙的作用

目的体外诱导角质形成细胞凋亡并研究人参皂甙对细胞凋亡的影响。方法将IFN-γ,Anti Fas Ab作用于体外培养角质形成细胞HaCaT诱导细胞凋亡,并检测人参皂甙对细胞凋亡的影响。结果50 ng/mL IFN-γ和25 ng/mL Anti Fas Ab共同作用可以诱导21.5%角质形成细胞HaCaT凋亡。8种人参皂甙中,Mx,CK和Re可逆转Anti Fas Ab诱导的HaCaT细胞凋亡,逆转率分别为18.4%,14.5%和9.6%。结论IFN-γ和Anti Fas Ab共同作用可诱导HaCaT细胞凋亡。人参皂甙Mx,CK和Re对Anti Fas Ab诱导的HaCaT细胞凋亡有一定的抑制作用。     

皮肤科学基础

20 0 12 834　 ︽-干扰素诱导角质形成细胞凋亡及其 Fas抗原表达研究 /张峻岭 (天津市长征医院皮肤科 )…∥中国皮肤性病学杂志 .- 2 0 0 1,15 (3) .- 16 3～ 16 4应用流式细胞仪测定角质形成细胞中亚二倍体细胞含量 ,片段化 DNA分析及 Annexin V法检测经 γ-干扰素诱导的角质形成细胞凋亡 ,并采用组化法、流式细胞仪测定经 γ-干扰素作用后角质形成细胞中 Fas抗原表达情况。结果表明 ,γ-干扰素可诱导角质形成细胞凋亡 ,并上调角质形成细胞中 Fas抗原的表达。提示 γ-干扰素可能通过上调角质形成细胞中 Fas抗原的表达 ,从而诱导角质形…     

γ—干扰素诱导角质形成细胞凋亡及其Fas抗原表达研究

目的：探讨γ－干扰素在银屑病发病中的作用。方法：流式细胞仪测定亚二倍体含量、片段化DNA分析、Annexin V凋亡检测法、免疫组织化学、间接免疫荧光流式细胞仪测定Fas抗原表达。结果：γ－干扰素上调角质形成细胞Fas抗原表达（P＜0．01）,诱导角质形成细胞凋亡（P＜0．01）,结论：γ－干扰素可能通过上调角质形成细胞Fas抗原表达,进而诱导角质形成细胞凋亡而参与银屑病发病。     

γ—干扰素诱导角质形成细胞凋亡及其Fas抗原表达研究

目的：探讨γ－干扰素在银屑病发病中的作用。方法 通过流式细胞仪测定角持形成细胞中亚二倍体细胞含量、片段化DNA分析及AnnexinV法检测经γ－干扰素诱导的角质形成细胞凋亡;采用组织化学、流式细胞仪测定经γ－干扰素作用后的角质形成细胞Fas抗原表达。结果 γ－干扰素上调角质形成细胞Fas抗原表达（P＜0．01）,诱导角质形成细胞凋亡（P＜0．01）。结论 γ－干扰素可能通过上调角质形成细胞Fas抗原表达,进而诱导角质形成细胞凋亡而参与银屑病发病。     

皮肤科学基础

20131668 PML基因诱导角质形成细胞凋亡及其Fas,Fasl和Bcl-2表达/王琼玉(西安交大医学院二附院皮肤科),马慧群,王世捷…//中国皮肤性病学杂志.-2013,27(6).-550~596通过流式细胞仪及AnnexinV法检测经PML基因转染的角质形成细胞凋亡;采用免疫组织化学、基因探针杂交测定经PML基因作用后的角质形成细胞Fas,Fasl和Bcl-2表达。结果:PML基因上调角质形成细胞Fas及Fasl表达(P<0.01),抑制Bcl-2基因表达,诱导     

β-溶血型链球菌诱发银屑病发病机理探讨

探讨β-溶血性链球菌诱发银屑病的机制。采用3H-TdR掺入法测定细胞增殖反应,AnnexinV法测定细胞凋亡,流式细胞仪检测细胞表面抗原表达。结果显示β-溶血性链球菌(SP)刺激淋巴细胞活化增殖,经SP活化的淋巴细胞培养上清液作用于角质形成细胞48h,可促进角质形成细胞增殖,诱导角质形成细胞表达HLA-DR和Fas抗原;再次加入上清液继续作用48h,则诱导角质形成细胞凋亡。SP作为超抗原首先活化T细胞,使之释放细胞因子,后者使角质形成细胞活化增殖,表达和抗原,继而诱导细胞凋亡,此过程可能是银屑病的主要发病机制之一。     

葡萄球菌性超抗原对银屑病患者外周血淋巴细胞产生白介素-2、γ-干扰素的影响

银屑病患者外周血淋巴细胞对葡萄球菌性超抗原（葡萄球菌肠毒素B，SEB)和链球菌超抗原刺激反应增强，且经SEB刺激的银屑病患者外周血淋巴细胞(PBLC)培养上清液可促进角质形成细胞增殖，表达HLA-DR和Fas抗原，继而诱导细胞凋亡。为了进一步探讨培养上清液对角质形成细胞的作用机制，笔者检测了SEB刺激前后的银屑病患者淋巴细胞培养上清液中白介素(IL）-2、γ-干扰素（IFN-γ)的水平变化，现将结果报告如下。     

金黄色葡萄球菌性超抗原诱发银屑病发病机理探讨

目的探讨金黄色葡萄球菌性超抗原诱发银屑病的机制。方法3H-TdR掺入法测定细胞增殖反应,亚二倍体细胞含量测定,片段化DNA分析,膜联蛋白V(AnnexinV)法测定细胞凋亡,流式细胞仪检测细胞表面抗原表达。结果金黄色葡萄球菌肠毒素B活化的淋巴细胞培养上清液作用于角质形成细胞48h,可促进角质形成细胞增殖,诱导角质形成细胞表达HLA-DR和Fas抗原;再次加入上清液继续作用48h,则诱导角质形成细胞凋亡（P<0.01）。结论金黄色葡萄球菌肠毒素B作为超抗原活化T细胞,使之释放细胞因子,后者使角质形成细胞首先活化增殖,继而凋亡。     

干扰素诱导蛋白16在银屑病患者皮损中的表达及其调控

目的研究干扰素诱导蛋白16(interferon inducible protein 16,IFI16)在银屑病中的表达及分布,探讨其在银屑病发病中的作用。方法采用RT-PCR、免疫组化的方法分别检测10例银屑病患者皮损和10例健康人皮肤组织真皮与表皮中IFI16的表达情况;采用RT-PCR、Western印记方法检测IFN-γ,TNF-α,IL-17刺激前后Ha Ca T细胞中IFI16的表达情况。结果银屑病患者皮损处IFI16的表达明显高于健康人皮肤,并且以表皮表达为主,免疫组化结果显示IFI16主要定位于银屑病患者的角质形成细胞中。银屑病相关细胞因子IFN-γ,TNF-α和IL-17可以上调角质形成细胞中IFI16的表达水平。结论IFI16在银屑病患者皮损处表达明显高于正常人皮肤,并且IFN-γ,TNF-α和IL-17可以诱导其表达上调,可能是银屑病角质形成细胞的活化机制之一。     

皮肤科学基础

20 0 10 0 0 1　影响新生鼠角质形成细胞及成纤维细胞Fas及其配体表达的因子 /贺为东 (中国医大一院皮肤科 )…∥中华医学杂志 (英文版 ) .- 2 0 0 0 ,113(9) .- 833～ 835以 RNase防护法及流式细胞分析法检测角质形成细胞和成纤维细胞 Fas及 Fas配体 (Fas L)的表达 ,并观察了维甲酸 (RA)及地塞米松 (Dex)对其表达的影响。结果发现 Fas m RNA被新生鼠角质形成细胞表达 ,全反式维甲酸上调而 Dex下调。RA和 Dex同时也增加角质形成细胞 Fas蛋白的表达。新生鼠角质形成细胞表达 Fas L m RNA,RA和 Dex对其都有上调作用。新生鼠成纤维…     

New fillers for the new man

ABSTRACT:  Two new collagen-based lidocaine-containing dermal fillers, ArteSense™/ArteFill™ (Artes Medical, San Diego, CA) and Evolence® (Colbar LifeScience Ltd., Herzliya, Israel), have proved to be of particular interest to men, many of whom seek a long-lasting or permanent correction. ArteFill™ has been available in the United States since 2006, and it is expected that Evolence® will reach the American market in 2008. The properties of the two products will be described, and experience based on the administration of many hundreds of syringes of both products by a Canadian dermatologist will be detailed here, with tips and precautions to optimize patient outcomes.     

Outcomes and adverse effects of ablative vs nonablative lasers for skin resurfacing: A systematic review of 1093 patients

It is generally believed that ablative laser therapies result in prolonged healing and greater adverse events when compared with nonablative lasers for skin resurfacing. To evaluate the efficacy of ablative laser use for skin resurfacing and adverse events as a consequence of treatment in comparison to other modalities, a PRISMA‐compliant systematic review (Systematic Review Registration Number: 204016) of twelve electronic databases was conducted for the terms “ablative laser” and “skin resurfacing” from March 2002 until July 2020. Studies included meta‐analyses, randomized control trials, cohort studies, and case reports to facilitate evaluation of the data. All articles were evaluated for bias. The search strategy produced 34 studies. Of 1093 patients included in the studies of interest, adverse events were reported in a total of 106 patients (9.7%). Higher rates of adverse events were described in nonablative therapies (12.2% ± 2.19%, 31 events) when compared with ablative therapy (8.28% ± 2.46%, 81 events). 147 patients (13.4%) reported no side effects, 68 (6.22%) reported expected, transient self‐resolving events, and five (0.046%) presented with hypertrophic scarring. Excluding transient events, ablative lasers had fewer complications overall when compared with nonablative lasers (2.56% ± 2.19% vs 7.48% ± 3.29%). This systematic review suggests ablative laser use for skin resurfacing is a safe and effective modality to treat a range of pathologies from photodamage and acne scars to hidradenitis suppurativa and posttraumatic scarring from basal cell carcinoma excision. Further studies are needed, but these results suggest that ablative lasers are a superior, safe, and effective modality to treat damaged skin.     

Genetic alterations in RAS‐regulated pathway in acral lentiginous melanoma

Studies integrating clinicopathological and genetic features have revealed distinct patterns of genomic aberrations in Melanoma. Distributions of BRAF or NRAS mutations and gains of several oncogenes differ among melanoma subgroups, while 9p21 deletions are found in all melanoma subtypes. In the study, status of genes involved in cell cycle progression and apoptosis was evaluated in a panel of 17 frozen primary acral melanomas. NRAS mutations were found in 17% of the tumors. In contrast, BRAF mutations were not found. Gains of AURKA gene (20q13.3) were detected in 37.5% of samples, gains of CCND1 gene (11q13) or TERT gene (5p15.33) in 31.2% and gains of NRAS gene (1p13.2) in 25%. Alterations in 9p21 were identified in 69% of tumors. Gains of 11q13 and 20q13 were mutually exclusive, and 1p13.2 gain was associated with 5p15.33. Our findings showed that alterations in RAS‐related pathways are present in 87.5% of acral lentiginous melanomas.     

Self‐resolving superficial primary cutaneous mucormycosis in a 7‐week‐old infant

A 7‐week‐old girl, born at 30 weeks' gestational age, presented to clinic for evaluation of a crop of vesicular lesions that were noted after removal of a bandage that had been in place for 4 days. A punch biopsy of the lesion revealed fungal elements that were later identified as Rhizopus spp. The lesion began to self‐resolve, and no further treatment was needed, with full resolution of the lesion by 1 month after presentation. Clinicians should be aware of the variable presentations of mucormycosis and consider fungal infection in the differential diagnosis when evaluating vulnerable patients with skin eruptions.     

Multiple New-Onset Subcutaneous Nodules of the Upper Extremity Digits: Giant Cell Tumor of Tendon Sheath and Lipoma

A black woman with the concurrent onset of two subcutaneous nodules located on the digits of her upper extremities is described. Initially, a single systemic disorder was considered; yet, the lesions differed in morphology and consistency. Microscopic examination of the nodules showed a giant cell tumor of tendon sheath and a lipoma. Although Occam's “razor” suggests that multiple lesions in the same person are more likely to represent variable manifestations of a single disorder than several different diseases in that individual, the simultaneously appearing lesions in this patient represented two different conditions.