Effect of Nephrotoxins on Tubulointerstitial Injury and NF-κB Activation in Adriamycin Nephropathy

In a previous study we found that an episode of acute subclinical nephrotoxicity with gentamicin (G) (but not that induced by another proximal tubular cell nephrotoxin: ferric nitrilotriacetate, FeNTA), paradoxically reduced the progression of renal function and injury in uninephrectomized rats with nephrotic glomerular disease due to Adriamycin nephropathy (AN). Here, we hypothesized that subclinical exposure to G reduces early renal cortical tubulointerstitial inflammation and NF-κB activation in AN. To test this hypothesis, male Wistar rats with established AN received either G (10, 40, or 80 mg/kg by daily s.c.i. for 3 days), FeNTA (1.25, 5, or 10 mg/kg by a single i.p.i.), or vehicle (n = 8 per group), 13 to 15 days after disease induction. Although G and FeNTA caused acute tubular necrosis in a dose-dependant manner (day 17), only the highest doses (10 mg/kg and 80 mg/kg) produced an acute elevation in the serum creatinine. On day 33, chronic tubulointerstitial inflammation (tubular atrophy, interstitial ED-1 + /CD8 + cell accumulation) and NF-κB activation were exacerbated only in the groups that caused functional nephrotoxicity. These data suggest that: 1) the protective effect of subclinical G nephrotoxicity in chronic AN does not involve early changes in interstitial inflammation or NF-κB activation; and 2) a single episode of G exposure must be accompanied by clinically apparent nephrotoxicity in order to accelerate progression in a nonuremic model of chronic glomerular disease.     

NF-κB与骨髓瘤肾病

NF—kB是一类普遍存在的转录因子，在细胞增殖、生长分化、细胞周期以及凋亡中发挥着重要的调节作用，在淋巴系恶性肿瘤的发病中也起一定作用。本文就NF—kB在骨髓瘤肾病（myeloma nephropathy）中的作用机制以及抗NF—kB治疗的新进展作一综述。     

NF-κB与肾脏疾病

Ｎ Ｆ－ ｋ Ｂ是一种具有多向性调节作用的核转录因子，与许多基因特别是免疫炎症相关基因的转录调节密切相关，其在肾脏疾病中的作用已日益引起人们的关注。本文就近年来 Ｎ Ｆ－ ｋ Ｂ与肾脏疾病的相关研究进展作一综述。     

NF-κB与骨髓瘤肾病

NF-κB是一类普遍存在的转录因子,在细胞增殖、生长分化、细胞周期以及凋亡中发挥着重要的调节作用,在淋巴系恶性肿瘤的发病中也起一定作用。本文就NF-κB在骨髓瘤肾病(myeloma nephrop-athy)中的作用机制以及抗NF-κB治疗的新进展作一综述。     

NF-κB与肝细胞凋亡

NF-κB广泛存在于肝脏各种细胞中,通过调节众多基因的转录参与肝脏的生理、病理过程。近年来研究证明,NF-κB与肝细胞凋亡关系密切。本文就NF-κB及其在肝细胞凋亡中的作用作一综述。1NF-κB家族组成及其活化和调控NF-κB属NF-κB/Rel蛋白家族,包括Rel A(P56)、RelB、C-Rel(C亲     

NF-κB与泌尿系肿瘤

ＮＦ κＢ又称核转录因子 ,具有和某些基因上启动子区的固定核苷酸序列结合而启动基因转录的功能。因其最初被发现是Ｂ细胞的核抽提物中一种能与免疫球蛋白κ链基因特异性结合的核蛋白因子 ,故名ＮＦ κＢ。在非激活条件下 ,ＮＦ κＢ在大多数细胞类型的细胞浆内与其抑制蛋白ＩκＢ家族紧密结合而呈无活性状态。它可以被许多刺激剂激活 ,包括细胞因子 ,促有丝分裂因子 (ｍｉｔｏｇｅｎｓ) ,病毒 ,双股ＲＮＡ ,氧化脂质体 ,紫外线 ,纤维蛋白原等。被激活的ＮＦ κＢ与ＩκＢ解离 ,转入核内与靶基因的ｋＢ基序结合 ,增强其表达。这些靶基因包…     

NF-κB激活与危重病

转录因子NF-KB活性增加可能是感染、创伤、失血、缺血.再灌注等引起多器官功能衰竭的中心环节,可以诱导危重病人发生急性炎症反应综合征。NF-KB在调控免疫反应中具有极其重要的作用,有必要进一步认识其调控机制,以寻找危重病人急性炎症损伤的最佳治疗途径。     

Activation of NF-κB pathway in oral buccal mucosa during small intestinal ischemia-reperfusion injury

BackgroundIntestinal ischemia-reperfusion injury induces intestinal mucosal barrier disruption, systemic inflammatory response syndrome, multiorgan failure, and death. The major pathway for the systemic inflammatory responses depends on nuclear factor kappa B (NF-κB). However, direct measuring of NF-κB in injured tissues is not routinely available. Our aim was to determine whether NF-кB pathway in buccal mucosa is activated during intestinal ischemia-reperfusion injury.Materials and methodsMale Sprague-Dawley rats were prepared for the animal experiment. Superior mesenteric artery (SMA) was exposed and clamped for 30 min in the intestinal ischemia-reperfusion (IR) group. SMA was exposed only in control group. Serum, buccal mucosa, and small intestinal mucosa were harvested in 90 min after reperfusion in IR or 120 min after SMA exposure in control group. Serum cytokine levels and tissue NF-κB pathway activities were measured.ResultsSerum TNF-α (5.49 ± 2.72 versus 1.77 ± 1.20 pg/mL, P = 0.002) and interleukin-6 (232.32 ± 29.98 versus 115.92 ± 17.81 pg/mL, P = 0.002) levels were significantly higher in IR than control group. Intestinal mucosal cytoplasmic phosphorylated inhibitor kappa B (IκB)/IκB ratio, nuclear NF-κB expression, and NF-κB DNA-binding activity were significantly higher in IR than control group. Buccal mucosal cytoplasmic phosphorylated IκB/IκB ratio, nuclear NF-κB expression, and NF-κB DNA-binding activity were also higher in IR than control group.ConclusionBuccal mucosal NF-κB pathway was activated by intestinal ischemia-reperfusion injury. The present study suggests that buccal mucosal may be considered as an indicator for the assessment of intestinal ischemia-reperfusion injury.     

NF-κB与肾间质纤维化

肾间质纤维化是各种肾脏疾病发展到终末期肾衰竭的最终结果,其主要病理改变表现为肾间质成纤维细胞的增生和细胞外基质的过度积聚.大量的临床和动物实验表明,各种肾脏疾病肾功能恶化的主要程度取决于肾间质受损的程度,因此,肾间质纤维化这些年来倍受人们重视,相关研究进展很快.NF-κB是一种多向性调节作用的核转录因子,参与了许多肾脏疾病的发生发展过程.本文就近些年来国内外NF-κB在肾间质纤维化的相关研究进展作一综述.     

NF-κB与慢性移植物血管病

核因子-κB(NF-κB)的活化在慢性移植物血管病(CAV)的发生、发展过程中至关重要.以NF-κB作为预防、治疗CAV的靶点所进行的研究已取得了一定进展.     

NF-κB与慢性移植物血管病

核因子-κB(NF-κB)的活化在慢性移植物血管病(CAV)的发生、发展过程中至关重要。以NF-κB作为预防、治疗CAV的靶点所进行的研究已取得了一定进展。     

Metabolomic Analysis Reveals that SPHK1 Promotes Oral Squamous Cell Carcinoma Progression through NF-κB Activation

Annals of Surgical Oncology - Metabolic disorders are significant in the occurrence and development of malignant tumors. Changes of specific metabolites and metabolic pathways are molecular...     

糖尿病与核转录因子NF-κB

核转录因子-κB(NF-κB)作为一种转录调节因子在体内各组织细胞中广泛存在，调控多种细胞因子和炎性介质的基因表达，调节免疫反应，与多种疾病的发生发展密切相关。了解其生物学特点，与肾脏病的关系。特别是近年来在糖尿病及糖尿病肾病发病中的作用，有助于该病的防治。     

核转录因子NF-κB的临床意义

近年来 ,有关核转录因子ＮＦ -κＢ的研究成果在国际上引起强烈反响 ,大量有关ＮＦ -κＢ的报告均发表在国际重要杂志上 ,其研究的深度和广度正越来越令人注目。其研究的成果可能为治疗炎症性病变、肿瘤及重症疾病带来根本性的改变。现将ＮＦ -κＢ的基本情况及其同临床的联系扼要地介绍给中国的外科医师 ,希望我们能在此领域里共同努力 ,取得成功。1　何为ＮＦ -κＢＮＦ -κＢ是一组近年来发现的核转录调节因子。虽然近期提纯的转录调节因子并不少 ,但ＮＦ -κＢ显得尤为重要 ,因为它在调节重症疾病的免疫调节基因方面有特殊的作用 ,尤其…     

NF-κB与心肌缺血再灌注损伤

NFκB是一组存在于细胞和病毒中调控许多基因表达的重要转录调节因子之一。充分认识NFκB在心肌缺血再灌注损伤、心肌预处理及细胞凋亡中的重要作用,有助于为防治心肌缺血再灌注损伤提供新的研究方向。     

NF-κB及iNOS与肾脏疾病

NF κB是一种多向性转录调节蛋白 ,参与多种细胞因子和炎症介质基因表达的转录调控 ,与多种免疫炎症性疾病有着密切关系。在哺乳动物细胞中 ,一氧化氮合酶家族中诱生型一氧化氮合酶在致炎性细胞因子或脂多糖刺激下 ,其催化合成的一氧化氮在许多免疫炎症性疾病中发挥着重要作用。因此 ,阐述NF κB及诱生型一氧化氮合酶基因在NF κB参与诱导激活下催化合成的一氧化氮在肾脏疾病中的作用 ,有着重要的理论意义和潜在的临床应用价值。