IFT20蛋白在不同肿瘤组织中表达的研究

  目的  探讨IFT20（Intraflagellar Transport,IFT）蛋白在人卵巢癌、肺癌、胰腺癌、小肠癌、前列腺癌、骨癌、胃癌和乳腺癌癌组织和癌旁组织的表达分布情况,为人类肿瘤的诊断和治疗寻找新的靶点。  方法  采用免疫组织化学方法,研究IFT20蛋白在人的八种癌组织和癌旁组织的表达。  结果  结果发现,IFT20蛋白在卵巢癌组织中大量表达,在卵巢癌癌旁组织低量表达。IFT20蛋白在小肠癌,胃癌和乳腺癌组织中中量表达,在小肠癌,胃癌和乳腺癌癌旁组织中低量表达。IFT20蛋白在胰腺癌,骨癌,肺癌癌组织中低量表达,在胰腺癌、骨癌、肺癌癌旁组织中几乎不表达。IFT20蛋白在前列腺癌组织和癌旁组织中均未见表达。  结论  实验结果证实,IFT20蛋白可能经过多种途径直接或间接调节卵巢癌、肺癌、胰腺癌、小肠癌、骨癌、胃癌和乳腺癌的发生,发展过程,并且IFT20可能是卵巢癌、肺癌、胰腺癌、小肠癌、骨癌、胃癌和乳腺癌肿瘤药物治疗的潜在靶点。      

吐温80及温热对荷瘤鼠肿瘤坏死因子的影响

ＢＡＬＢ／Ｃ小鼠经腹接种Ｂ１６黑色素瘤细胞建立荷瘤鼠模型，经静脉注射吐温８０及腹部４１℃温热，于处理后１周和２周测定肿瘤坏死因子（ＳｅｒｕｍＴｕｍｏｒＮｅｅｒｏｓｉｓＦａｅｔｏｒ，ＳＴＮＦ）活性及血清唾液酸含量，并观察作用后荷瘤鼠死亡率，结果显示，荷瘤鼠ＳＴＮＦ活性及唾液酸含量持续保持相对高水平（正常ＢＡＬＢ／Ｃ鼠ＳＴＮＦ活性检测不出；唾液酸含量〈５０ｍｇ／ｍｌ）。单独以吐温８０处理和吐温８０合并     

KU80蛋白在非小细胞肺癌中表达的临床意义

目的探讨KU80蛋白在非小细胞肺癌预后及其治疗效果中表达的临床意义。方法采用MaxvisionTM免疫组化法检测88例非小细胞肺癌的KU80阳性表达率的情况。结果 88例非小细胞肺癌KU80蛋白阳性表达率为54.5%,其阳性表达率与肿瘤的大小、淋巴结转移和治疗效果密切相关,其中肿瘤直径大于2cm组与小于2cm组的KU80阳性表达率结果差别具有非常显著性意义,有淋巴结转移组与无转移组KU80阳性表达率结果差别具有非常显著性意义,进展组、缓解组与稳定组KU80阳性表达率结果差别具有显著性意义;与病理分型和TNM分期无关。结论 KU80蛋白水平能预测非小细胞肺癌预后及其治疗效果,可临床指导个体化治疗。     

IA期非小细胞肺癌的肿瘤大小对其预后的影响

背景与目的 肿瘤大小对预后的影响在肺癌的不同分期组间已明确，但其在同一分期中，尤其是直径小于3cm的肿瘤中对预后的影响尚未明确。本研究的目的是探讨IA期非小细胞肺癌中肿瘤大小对预后的影响。方法 回顾性分析自1995年1月至2003年12月我院胸外科手术治疗IA期非小细胞肺癌142例，用Kaplan-Meier生存曲线统计不同肿瘤大小患者的生存率，并对发病年龄、性别、病理类型、肿瘤大小、手术方式以及是否接受放化疗等因素进行COX回归比例风险模型多因素分析。结果 全组肿瘤直径≤2．0cm者60例，2．1～3．0cm者82例。全组3年、5年生存率分别为84．41％、70．89％，其中肿瘤直径≤2．0cm组分别为94．91％和81．40％，2．1～3．0cm组分别为82．18％和64．91％（P=0．0353）。单因素及多因素分析均显示，肿瘤大小为独立的预后因素。结论 肿瘤大小是IA期非小细胞肺癌的独立预后因素。临床工作中应进一步提高影像学诊断水平，使患者获得更早期的治疗。     

人脐带血间充质干细胞对人胃癌MGC80-3细胞生物学行为的影响

目的：研究人脐带血间充质干细胞（ HUCB-MSC）对人胃癌MGC80-3细胞体外细胞外基质成分的影响,探讨其对胃癌细胞生物学行为的影响。方法：原代培养人脐带血间充质干细胞,流式细胞仪进行细胞表型鉴定。采用流式细胞仪半定量检测基质金属蛋白酶-7（ MMP-7）及基质金属蛋白酶抑制剂-1（ TIMP     

细胞粘附蛋白与肺癌相关性研究

目的：细胞粘附蛋白（Ａｄｈｅｓｉｏｎ Ｐｒｏｔｅｉｎ,ＡＰｓ）是一种广泛分布于体内的糖蛋白,种类繁多。此研究分析了ＡＰｓ中的血管性假血友病因子相关抗原（ｖＷＦ：Ａｇ）、纤维连结蛋白（Ｆｎ）及纤维蛋白原（Ｆｇ）三项指标与肺癌的相关性。方法：上述三项指标均采用免疫火箭电泳方法,对２１５例肺癌患者和４８例肺良性疾病患者进行检测。结果：肺癌患者血浆中上述三项水平均高于肺良性疾病,并有统计学意义,Ｐ〈０．０     

肿瘤微环境对胃癌免疫治疗的影响

胃癌是消化系统死亡率较高的恶性肿瘤,手术切除、放疗、化疗等仍是目前主要的治疗手段,但效果并不理想。随着免疫学研究的发展,免疫治疗成为肿瘤综合治疗中研究的热点,许多新兴的手段如肿瘤疫苗、免疫卡控点治疗等展现出良好的应用前景。由于胃癌发病机制复杂,肿瘤异质性明显,还有很多问题亟待解决,肿瘤微环境是影响肿瘤的发生、发展、转移的重要因素,本文就肿瘤微环境对胃癌免疫治疗的影响方面的研究成果作一综述。     

ⅠA期非小细胞肺癌的肿瘤大小对其预后的影响

背景与目的肿瘤大小对预后的影响在肺癌的不同分期组间已明确,但其在同一分期中,尤其是直径小于3 cm的肿瘤中对预后的影响尚未明确.本研究的目的是探讨Ⅰ A期非小细胞肺癌中肿瘤大小对预后的影响.方法回顾性分析自1995年1月至2003年12月我院胸外科手术治疗Ⅰ A期非小细胞肺癌142例,用Kaplan-Meier生存曲线统计不同肿瘤大小患者的生存率,并对发病年龄、性别、病理类型、肿瘤大小、手术方式以及是否接受放化疗等因素进行COX回归比例风险模型多因素分析.结果全组肿瘤直径≤2.0 cm者60例,2.1～3.0 cm者82例.全组3年、5年生存率分别为84.41%、70.89%,其中肿瘤直径≤2.0 cm组分别为94.91%和81.40%,2.1～3.0 cm组分别为82.18%和64.91%(P=0.0353).单因素及多因素分析均显示,肿瘤大小为独立的预后因素.结论肿瘤大小是Ⅰ A期非小细胞肺癌的独立预后因素.临床工作中应进一步提高影像学诊断水平,使患者获得更早期的治疗.     

肿瘤局部因素对非小细胞肺癌放疗疗效的影响

目的回顾性分析根治性放疗的非小细胞肺癌(NSCIC)病例，探讨肿瘤局部因素对放疗疗效的影响。方法接受根治性放疗并经病理或细胞学证实的Ⅰ～Ⅲb期NSCIC 75例。常规放疗39例，适形放疗36例，总剂量60～70Gy，30～35分次，6～7周完成。由CT扫描测量肿瘤最大直径及体积，对相关指标进行单因素、多因素分析，并用预后指数模型综合评价放疗疗效。结果完全缓解(CR)率33．3％(25／75)，有效率(CR PR)率85．3％(64／75)，无变化加病变进展(NC PD)率14．7％(11／75)。中位生存期13．8个月，1．0、1．5年生存率分别为54．7％、27．5％。单因素及多因素分析显示原发肿瘤直径较小、体积较小、T分期较早、总治疗时间较短和放射性肺炎较轻者预后较好。预后指数能够更敏感地预测放疗疗效。结论原发肿瘤直径、肿瘤体积、T分期、总治疗时间、急性放射性肺炎有可能成为放疗NSCLC的独立影响因素，预后指数模型能够显著提高多指标联合的预测价值。     

血清肿瘤标志物与肺癌病理类型的相关性研究

目的 探讨血清肿瘤标志物与肺癌病理类型的相关性.方法 收集143例肺癌患者的病历资料,根据病理类型将患者分为小细胞肺癌(SCLC)组(n=19)和非小细胞肺癌(NSCLC)组(n=124),NSCLC组患者中腺癌89例,鳞状细胞癌35例.比较治疗前SCLC组和NSCLC组患者的血清神经元特异性烯醇化酶(NSE)、胃泌素释放肽前体(ProGRP)、细胞角质蛋白19片段抗原21-1(CYFRA21-1)、癌胚抗原(CEA)、鳞状细胞癌抗原(SCC-Ag)水平,比较治疗前NSCLC组中腺癌和鳞状细胞癌患者的血清NSE、ProGRP、CYFRA21-1、CEA、SCC-Ag水平,采用受试者工作特征(ROC)曲线及曲线下面积(AUC)分析各血清肿瘤标志物对SCLC与NSCLC及NSCLC分型的诊断价值,分析各血清肿瘤标志物与肺癌病理类型的相关性.结果 NSCLC组患者的血清NSE、ProGRP水平均明显低于SCLC组患者,血清CYFRA21-1、SCC-Ag水平均明显高于SCLC组患者,差异均有统计学意义(P﹤0.01);NSCLC组和SCLC组患者的血清CEA水平比较,差异无统计学差异(P﹥0.05).NSCLC组中腺癌患者的血清NSE、ProGRP、CYFRA21-1、CEA水平均明显高于鳞状细胞癌患者(P﹤0.01),NSCLC组中腺癌和鳞状细胞癌患者的血清SCC-Ag水平比较,差异无统计学意义(P﹥0.05).对于NSCLC与SCLC分型,NSE、CYFRA21-1、SCC-Ag具有中等诊断价值(AUC=0.833、0.765、0.794,P﹤0.01),ProGRP具有较高诊断价值(AUC=0.978,P﹤0.01);对于NSCLC中腺癌和鳞状细胞癌分型,CYFRA21-1、CEA具有较高诊断价值(AUC=0.964、0.912,P﹤0.01),NSE具有中等诊断价值(AUC=0.727,P﹤0.01),ProGRP具有较低诊断价值(AUC=0.659,P﹤0.01).血清肿瘤标志物ProGRP与肺癌病理类型高度相关(r=-0.848,P﹤0.05),NSE与肺癌病理类型中度相关(r=-0.755,P﹤0.05),CYFRA21-1与肺癌病理类型低度相关(r=-0.313,P﹤0.05).结论 血清肿瘤标志物水平与肺癌病理类型具有明确相关性,可为肺癌的病理类型诊断提供参考.     

The expression of metabolism-related proteins in phyllodes tumors

The purpose of this study was to investigate the association between the expression of hypoxia-inducible factor (HIF)-1α, insulin-like growth factor (IGF)-1, glucose transporter 1 (Glut-1), carbonic anhydrase IX (CAIX), and monocarboxylate transporter (MCT)4, which are metabolism-related proteins in phyllodes tumors (PTs), and clinicopathologic factors and its implication. We used tissue microarrays to analyze 207 PTs and performed immunohistochemical staining against the glycolysis-related molecules HIF-1α, IGF-1, Glut-1, CAIX, and MCT4. We then compared the immunohistochemical results and clinicopathologic parameters. The expressions of HIF-1α, Glut-1, CAIX, and MCT4 in the stromal component of PTs increased (P?=?0.019, P?<?0.001, P?=?0.045, and P?<?0.001, respectively) with increasing tumor grade. According to univariate analysis, factors associated with shorter disease-free survival were Glut-1 expression (P?=?0.001) and MCT4 expression (P?<?0.001) in the stromal component, and the factors associated with shorter overall survival were IGF-1 expression (P?=?0.012), Glut-1 expression (P?<?0.001), CAIX expression (P?=?0.039), and MCT4 expression (P?<?0.001) in the stromal component. Our investigation of stromal expression of the metabolism-related proteins HIF-1α, IGF-1, Glut-1, CAIX, and MCT4 revealed that, as the PT grade increased, the stromal expression of HIF-1α, Glut-1, CAIX, and MCT4 significantly increased. This result suggested that increasing PT grade is associated with increased glycolysis in the stromal component.     

The effects of vasodilating drugs on pH in tumors

Hydralazine has been used widely to reduce tumor blood flow and thereby to induce hypoxia and to reduce extracellular pH (pHe) in tumors. Here we have investigated and compared the effects of the vasodilating drugs hydralazine, captopril, nifedipine, prazosin, sodium nitroprusside, and labetalol to reduce pHe in EMT-6 and KHT tumors of mice and to cause antitumor effects. After a single injection, captopril was most effective in reducing pHe in EMT-6 tumors with a decrease in mean pHe from 6.93 to 6.67 at 2 h after injection, while nifedipine was most effective for KHT tumors with a decrease in mean pHe from 6.96 to 6.75 at 1 h after injection. During 72 h of chronic administration into mice bearing tumors, nifedipine was ineffective in reducing pHe, but both captopril and hydralazine caused a small but significant reduction of pHe. Captopril caused significant delay in growth of the tumors, but had only a small effect on clonogenic cell survival. Captopril appears to be the most effective vasodilating drug to enhance tumor acidity.     

The expression of glutamine-metabolism-related proteins in breast phyllodes tumors

The aim of this study was to investigate the expression of glutamine-metabolism-related proteins according to the histologic grade of phyllodes tumors (PTs) and to assess its clinical implication. We generated tissue microarrays of 224 PTs and performed immunohistochemical staining and western blot analysis of glutamine-metabolism-related molecules, including GLS1, GDH, and ASCT2. The associations between immunohistochemical results and clinicopathologic parameters were evaluated. The expression of GLS1 (p?<?0.001), GDH (p?<?0.001), and ASCT2 (p?=?0.005) in stromal components significantly increased with worsening PT histological grade. GDH expression in epithelial components significantly increased in high-grade PT (p?=?0.026). In western blot, stromal expression of GLS1, GDH, and ASCT2 increased as histologic grade increased. By univariate analysis, stromal GLS1 expression (p?=?0.022) and stromal GDH expression (p?=?0.009) were independent predictors of shorter DFS. Stromal GLS1 expression (p?<?0.001) and stromal GDH expression (p?<?0.001) were independent predictors of shorter OS. This study demonstrated that the stromal expression of the glutamine-metabolism-related proteins GLS1, GDH, ASCT2 increases with worsening histological PT grade.     

核糖体蛋白与肿瘤

核糖体由核糖体蛋白和核糖体RNA组成,是蛋白质生物合成的重要场所.核糖体蛋白参与蛋白质的翻译过程,核糖体蛋白和核糖体RNA的表达紊乱将激活核糖体蛋白的核糖体外功能,该功能在肿瘤的发生发展过程中发挥重要作用.核糖体蛋白有望作为生物标志物或治疗靶点用于肿瘤的早期诊断和治疗中.     

The histological types and the effects of radiation on tumors

The effects of radiation on tumors should be divided into radiosensitivity and radioresponsiveness. The local effects of radiation are not always consistent with curability. Though local control is one requirement to attain cure, the primary factor that affects long-term survival is the natural history of the tumor. The effects of irradiation can not be predicted only by determining the histological type of tumor. However, the natural history of the tumor can be understood by careful observation of the stroma in the tumor and condition of the host. As the result, radiocurability can be predicted to some extent.     

Effect of chemotherapy on cytosol proteins of ovarian tumors

Cytosol proteins of malignant ovarian tumors were studied with one- and two-dimensional gel electrophoresis, prior to and after effective chemotherapy with cisplatin and taxotere. Effective chemotherapy caused the bands and spots of 29, 36 and 50-55 kDa to fade significantly or disappear completely. It is suggested that protein 36 kDa is the proliferating nuclear cell antigen while one of the proteins 50-55 kDa--betatubulin. These proteins may serve as additional markers of ovarian tumor sensitivity to chemotherapy.     

骨形态发生蛋白与肿瘤

骨形态发生蛋白(BMP)是一种多功能的细胞因子,不仅可以促进新骨形成,而且参与调控细胞生长、分化、迁移、凋亡以及胚胎和器官发生等过程.研究显示BMP还与肿瘤的发生和转移密切相关,在肿瘤治疗中具有重要的应用价值.     

微小RNAs对胸腺上皮细胞肿瘤的影响及研究进展

胸腺作为机体重要免疫器官,参与机体多种免疫功能。胸腺瘤和胸腺癌是来源于胸腺上皮细胞的肿瘤,在我国前纵隔肿瘤中较为常见。微小RNA（microRNAs,miRNAs）是一类内源性非编码小分子单链RNA,长度约为22个氨基酸,参与转录后基因表达调控过程。近年来miRNAs的研究已成为各学科的研究热点,针对miRNAs的新型诊断方法与治疗手段不断在国内外学术期刊上报道。胸腺上皮细胞肿瘤由于常伴有副肿瘤综合征,使其在临床症状表现及治疗方面复杂多样,通过miRNAs与胸腺上皮细胞肿瘤的研究,有望从根源上找出胸腺肿瘤多样性的原因并找到有效治疗手段。本文对近几年miRNAs与胸腺上皮细胞肿瘤的研究进行文献复习,探讨miRNAs对胸腺上皮细胞肿瘤的影响及研究进展,并作一综述。