动态检测IL-6、CRP和PCT水平对急诊重症细菌感染患者的预后价值

目的 探讨白细胞介素-6(IL-6)、C反应蛋白(CRP)和降钙素原(PCT)在评估急诊重症细菌感染患者预后中的应用价值.方法 选取2018年1月至2019年1月急诊入亳州市人民医院的重症细菌感染患者60例.根据患者治疗后的预后情况,分为死亡组与存活组,对死亡组与存活组患者入院后血清IL-6、CRP和PCT水平进行动态...     

血清PCT、IL-6在脓毒症患儿中的水平变化及临床意义

目的研究血清降钙素原(PCT)、白细胞介素-6(IL-6)在脓毒症患儿中的水平变化及临床意义。方法选取2019年8月至2020年8月郑州大学附属儿童医院收治的90例疑似感染脓毒症患儿作为实验组,另取该院同期健康体检儿童80例作为对照组。根据快速序贯器官功能衰竭评分(qSOFA)将实验组分为非脓毒症组71例和脓毒症组19例;根据预后效果的不同将实验组分为预后良好组65例和预后不良组25例。各组受检者均接受血清IL-6、PCT水平的检测,分别比较实验组与对照组、非脓毒症组与脓毒症组、预后良好组与预后不良组的血清IL-6、PCT水平。结果各组血清IL-6、PCT水平经比较发现,实验组明显高于对照组(P<0.05);脓毒症组高于非脓毒症组(P<0.05),预后不良组高于预后良好组(P<0.05)。结论相比健康儿童,脓毒症患儿血清IL-6、PCT均处于较高水平,进而可加重患儿感染程度,致使对患儿病情造成不良影响,临床可通过其水平变化为患儿病情评估及预后提供参考依据,整体应用价值较高。     

新生儿败血症血清IL-6、IL-8、PCT检测及其临床意义

目的 :探讨新生儿败血症时血清IL -6、IL -8、PCT的变化。方法 :IL -6、IL -8用ELISA双夹心法 ,PCT用放射免疫法。结果 :新生儿败血症血清IL -6、IL -8、PCT明显升高。结论 :IL -6、IL -8、PCT可作为新生儿败血症的早期诊断指标     

脓毒症患者血清PCT、CRP、IL-6和IL-10水平检测及临床意义

目的:探讨脓毒症患者血清中降钙素原(PCT),C反应蛋白(CRP),白细胞介素-6(IL-6),白细胞介素-10(IL-10)的表达水平及临床诊断价值。方法:选取40例脓毒症患者作为脓毒症组,同期40例非感染性全身炎症反应综合征(SIRS)患者作为非脓毒症组;另选取同期40例健康体检者作为正常对照组;根据28 d后是否存活将脓毒症组分为存活组(n=24)和死亡组(n=16)。分析比较各组入院24 h内PCT、CRP、IL-6、IL-10的水平,并绘制工作特征曲线(ROC曲线)分析上述指标对脓毒症的诊断价值。结果:脓毒症组血清中PCT、CRP、IL-6、IL-10水平均显著均高于非脓毒症组和正常对照组(均P<0.01)。脓毒症组40例研究对象中死亡16(40.00%)例,死亡组血清中PCT、CRP、IL-6及IL-10水平均显著高于存活组,差异有统计学意义(t=3.23,P<0.01;t=2.26,P=0.03;t=2.39,P=0.02;t=2.19,P<0.05);ROC分析结果显示:PCT的诊断曲线下面积(AUC)最大为0.84,灵敏度为87.50%,特异度为81...     

血清PCT、CRP和IL-6检测在妇科盆腔脓肿中的临床应用价值

目的:探讨血清PCT、CRP和白细胞介素-6检测在妇科盆腔脓肿中的临床应用价值.方法:选取2018年1月—2019年10月我院妇科收治的盆腔炎症患者80例为观察对象,根据患者是否出现盆腔脓肿分为合并脓肿组和单纯炎症组,每组40例.所有患者入院时抽取静脉血进行PCT、CRP、白细胞、中性粒细胞百分比和白细胞介素-6检测....     

腹水PCT、IL-6水平检测在肝硬化腹水合并自发性腹膜炎诊断中的应用价值

目的：探讨腹水降钙素原（PCT）、白细胞介素-6(IL-6)水平检测在肝硬化腹水合并自发性腹膜炎（SBP）诊断中的应用价值。方法：回顾性分析2019年3月至2021年3月该院收治的45例肝硬化腹水合并SBP患者的临床资料,纳入SBP组;并收集同期收治的45例肝硬化腹水未合并SBP患者的临床资料,纳入非SBP组。比较两组腹水PCT、IL-6水平,并采用受试者工作特征曲线（ROC）分析PCT、IL-6水平单项及联合检测在肝硬化腹水合并SBP诊断中的价值。结果：SBP组腹水PCT、IL-6水平高于非SBP组,差异均有统计学意义（P<0.05）;ROC曲线结果显示,腹水PCT、IL-6水平单项及联合检测诊断肝硬化腹水合并SBP的曲线下面积分别为0.819、0.825、0.829,且肝硬化腹水患者腹水PCT、IL-6水平检测诊断SBP的最佳截断值分别取1.155 ng/mL、81.895 pg/mL。结论：腹水PCT、IL-6水平检测在肝硬化腹水合并SBP诊断中具有一定的应用价值。     

WBC、PCT和IL-6在老年下呼吸道感染患者中的临床应用

目的观察血清白细胞计数(WBC)、降钙素原(PCT)、白细胞介素6(IL-6)在老年下呼吸道感染患者中的水平变化,以讨论其临床意义。方法选择2011年12月至2012年12月都江堰市人民医院呼吸科收治的老年下呼吸道感染患者120例,检查患者血清WBC、IL-6以及PCT水平,比较治疗前后的变化。结果治疗后WBC、IL-6、血清PCT水平较治疗前均显著降低,差异有统计学意义(P<0.01)。中高危组PCT水平高于低危组[(0.35±0.18)μg/L比(0.19±0.07)μg/L],差异有统计学意义(P<0.01)。结论 WBC、PCT、IL-6可反映下呼吸道感染的严重程度,可以根据WBC、PCT以及IL-6水平指导临床使用抗生素,同时可以相应减少抗生素耐药情况。     

痰涂片联合CRP、PCT及IL-6水平检测对老年细菌性肺炎的诊断价值研究

目的 探讨痰涂片联合C反应蛋白（CRP）、降钙素原（PCT）及白细胞介素-6（IL-6）水平检测对老年细菌性肺炎的诊断价值。方法 选取2019年6月—2022年6月四川省第二中医医院100例老年细菌性肺炎患者作为细菌性肺炎组,另取同期60例老年病毒性肺炎患者作为病毒性肺炎组。两组均接受痰涂片及CRP、PCT及IL-6水平检测,对比两组检测结果,绘制受试者工作特征（ROC）曲线分析痰涂片联合CRP、PCT及IL-6水平检测对老年细菌性肺炎的诊断价值。结果 细菌性肺炎组痰涂片阳性率较病毒性肺炎组高（P <0.05）。细菌性肺炎组WBC、CRP、PCT、IL-6较病毒性肺炎组高（P <0.05）。经点二列相关性分析结果显示,WBC、CRP、PCT、IL-6水平与细菌性肺炎呈正相关（r =0.626、0.618、0.676和0.586,均P <0.05）。ROC曲线结果显示：痰涂片诊断细菌性肺炎的敏感性为0.870（95% CI：0.811,0.931）、特异性为0.867（95% CI：0.806,0.922）,CRP敏感性为0.800（95% CI：0.789,0.902）、特异性为0.883（95% CI：0.824,0.953）,PCT敏感性为0.850（95% CI：0.802,0.925）、特异性为0.917（95% CI：0.889,0.988）,IL-6敏感性为0.790（95% CI：0.725,0.865）、特异性为0.767（95% CI：0.712,0.864）,四者联合检测的敏感性为0.950（95% CI：0.845,0.978）、特异性为0.769（95% CI：0.713,0.878）。结论 痰涂片、CRP、PCT、IL-6水平可有效鉴别老年细菌性肺炎与病毒性肺炎,联合检测可进一步提高诊断效能,为后续治疗方案的制订提供参考依据。     

血清PCT和CRP及IL-6表达水平对诊断慢性乙肝合并细菌感染的意义

目的 探讨血清降钙素原(PCT)、C反应蛋白(CRP)及白细胞介素-6(IL-6)在慢性乙肝合并细菌感染中的表达意义.方法 收集漯河市中心医院2018年2月-2020年1月收治的慢性乙肝患者129例,基于病原学、症状、影像学诊断,分为乙肝细菌感染组57例,乙肝疑似细菌感染组30例,单纯慢性乙肝组42例;另收集同期体检中...     

PCT,IL-6及PLT对于鉴定血流感染病原菌的价值研究

目的:探讨降钙素原(PCT)、白细胞介素-6(IL-6)及血小板(PLT)在鉴别血流感染致病菌类型中的临床应用价值.方法:回顾性分析2019-2020年我院住院治疗的血流感染病例,根据血培养结果将其分为革兰氏阳性菌(G+)组和革兰氏阴性菌(G-)组,比较两组血清PCT、PLT、IL-6水平的差异,同时绘制ROC曲线并计...     

脐带血白介素-6和白介素-10水平在早产儿脓毒血症中的应用研究

目的探讨脐带血白介素-6和白介素-10水平在早产儿脓毒血症中的应用价值。方法回顾性分析2017年9月～2018年9月台州恩泽医疗中心(集团)恩泽医院新生儿科早产儿脓毒血症组(观察组)和早产儿非脓毒血症组(对照组)各50例,对两组脐带白介素-6和白介素-10水平进行检测,并对白介素-6和白介素-10水平与临床疗效进行相关性分析。结果两组脐带血白介素-6与白介素-10水平比较,差异有显著性(t=38.09、22.58,P0.01),早产儿脓毒血症不同疗效脐带血白介素-6与白介素-10水平比较,差异有显著性(F=63.95、44.70,P0.01)。结论脐带血白介素-6与白介素-10水平对早期识别早产儿脓毒血症和临床疗效判断具有重要意义。     

Amniotic fluid interleukin-6 and the risk of early-onset sepsis among preterm infants.

BACKGROUND: High concentrations of interleukin-6 (IL-6) have been demonstrated in amniotic fluid (AF) from women with intra-amniotic infection. Recent studies have reported that IL-6 levels in AF were related to an increase in neonatal morbidity; moreover, higher IL-6 plasma levels have been observed in neonates with sepsis. METHODS: A cohort study was carried out at the National Institute of Perinatology in Mexico City. Inclusion criteria were the following: 1) preterm singleton pregnancy; 2) intact membranes at time of enrollment, and 3) written informed consent. Women with other complications of pregnancy were excluded. Newborn sepsis during the first 72 h was defined as early-onset sepsis. Amniotic fluid was obtained at the moment of delivery. Amniotic fluid IL-6 (AF IL-6) was determined by enzyme-linked immunoassays. RESULTS: Ninety-three women met the criteria for enrollment in the study and 31 (33%) of their newborns had early-onset neonatal sepsis. The mean AF IL-6 in mothers of septic newborns was 5779 +/- 2804 pg/ml compared to 729 +/- 382 pg/ml in mothers with non-infected neonates (p < 0.001). AF IL-6 concentrations higher than 1250 pg/ml were significantly associated with early-onset sepsis (OR 33.3; 95% CI 9.4-117.3) (p < 0.001). Gestational age under 32 weeks was also associated with neonatal sepsis (OR 2.56; 95% CI 1.2-9) (p = 0.002). Women whose infants developed neonatal sepsis had a higher frequency of clinical chorioamnionitis (p = 0.02). CONCLUSIONS: IL-6 determination in AF may be a useful indicator to identify neonates with higher risk of in utero bacterial infection.     

Role of prophylactic surfactant in preterm infants

Background

Respiratory distress syndrome (RDS) in preterm neonates is caused by a deficiency or dysfunction of pulmonary surfactant. The physiological function of surfactant includes the ability to lower surface tension, as well as the ability to rapidly adsorb and spread. A wide variety of surfactant products have been formulated and studied in clinical trials. The present study was designed to find out whether prophylactic administration of surfactant leads to a significant decrease in the risk of neonatal mortality and neonatal morbidity.

Methods

This was an experimental study in which a total of 125 preterm newborns less than 34 weeks gestation were studied. One hundred preterm newborns (controls) less than 34 weeks gestation were managed in the conventional manner as per the existing protocols in the neonatal intensive care unit. Twenty-five consecutively delivered preterm newborns less than 34 weeks gestation were administered surfactant. Data regarding clinical outcomes including mortality and morbidity profile was collected and analysed.

Results

The mean duration of ventilation in the ventilated babies in the control group and the surfactant group was 129.8 ± 43 hours and 85.7 ± 46 hours, respectively; the difference being statistically significant. In the surfactant group, four babies (16%) died and in the control group, 27 babies (27%) died. The difference was not statistically significant. The number of babies developing retinopathy of prematurity and needing laser treatment for retinopathy of prematurity was greater in the surfactant group.

Conclusion

Prophylactic administration of surfactant in preterm newborns of gestational age <34 weeks is associated with a significant decrease in mean duration of ventilation and an increase in the incidence of retinopathy of prematurity.     

降钙素原在细菌感染性疾病中的诊断作用

目的:探讨血清降钙素原(PCT)在细菌感染性疾病中的诊断价值。方法:回顾性分析36例细菌培养阳性和40例细菌培养阴性住院患者的PCT、血白细胞(WBC)计数和C反应蛋白(CRP)检测结果,PCT检测采用半定量免疫色谱法。结果:2组PCT分级差异无统计学意义(P0.05)。细菌培养阳性组WBC和CRP均明显高于细菌培养阴性组(P0.01)。细菌培养阳性组PCT、WBC、CRP阳性率依次为36.11%、63.89%、97.22%,细菌培养阴性组PCT、WBC、CRP阳性率依次为25.00%、22.50%、72.50%,细菌培养阳性组WBC、CRP阳性率均明显高于细菌培养阴性组(P0.01),2组PCT阳性率差异无统计学意义(P0.05)。灵敏度依次为PCT 36.11%、WBC 63.89%、CRP 97.22%,特异度依次为PCT 75.00%、WBC 77.50%、CRP27.50%。结论:PCT在细菌感染诊断中有一定价值,与WBC和CRP联合检测能够更准确地判断细菌感染。     

Comparison of umbilical cord interleukin-6 in preterm infants with premature rupture of membranes and intact membranes

OBJECTIVE: To compare inflammatory mediators in the cord blood of premature newborn infants with premature rupture of membranes (PROM) and intact membranes. METHODS: Eighty-nine premature neonates with gestational age of 27-34 weeks that delivered in Ghaem Hospital in Mashhad, Iran from June 2005 to March 2006 were enrolled in a prospective observational study, and their umbilical cord plasma was collected at birth. They were allocated into 2 groups (45 patients with PROM, and 44 neonates with intact membranes). Interleukin-6 (IL-6) and C-reactive protein (CRP) levels were measured in cord plasma by the enzyme linked immunoassay (ELISA) method. RESULTS: Mean cord plasma IL-6 levels in preterm neonates with PROM was 205.71 pg/ml, and in neonates with intact membranes was 33.3 pg/ml for IL-6 (p=0.000). The mean cord blood CRP level in newborns with PROM was 10.2 microgram/ml, and in those with intact membranes was 1.6 microgram/ml (p=0.41). Early onset sepsis was more frequent in infants with PROM than premature infants with intact membrane (38% versus 10%, p=0.001). In neonates with PROM, the mean cord blood IL-6 level was significantly higher in septic newborns (414.28 verus 40.44 pg/ml, p=0.000). CONCLUSION: The premature newborn infants with PROM had increased IL-6 levels in cord blood, which was significantly higher in neonates that developed early onset sepsis.     

Serum procalcitonin and interleukin-6 levels may help to differentiate systemic inflammatory response of infectious and non-infectious origin

Objective To evaluate the efficacy of using procalcitonin (PCT) and interleukin-6 (IL-6) to differentiate sepsis from non-infectious systemic inflammatory response syndrome (SIRS). Methods We made a prospective study in a general intensive care unit at Peking Union Medical College Hospital. Twenty patients with sepsis and 31 patients with non-infectious SIRS were enrolled in this study. Serum concentrations of PCT, IL-6 and C-reactive protein (CRP) were determined within 24 h after clinical onset of sepsis or non-infectious SIRS. Leukocyte count, percentage of neutrophils, and absolute neutrophil count, as well as maximal body temperature were also recorded.Results Serum concentrations of PCT, IL-6, and CRP, as well as maximal body temperature, were significantly higher in septic patients ［3.6 (1.8, 27.5) μg/L, 810±516 ng/L, 180±108 g/L, 38.6±1.2℃］ than non-infectious SIRS patients ［0.5 (0.2, 1.8) μg/L, 235±177 ng/L, 109±70 g/L, 37.9±0.9℃］. IL-6 and PCT exhibited the best discriminative power between sepsis and non-infectious SIRS, with sensitivity above 80% and specificity above 70%. A sepsis score with combination of IL-6 and PCT showed the best discriminative power with the area under the receiver operating characteristic curve of 0.923.Conclusions Assessing IL-6 and PCT levels are more reliable ways to differentiate sepsis from non-infectious SIRS, compared with conventional inflammatory parameters.     

血清降钙素原在诊断尿脓毒血症中的意义

目的：探讨血清降钙素原（procalcitonin,PCT）在诊断尿脓毒血症中的临床意义。方法：41例尿脓毒血症患者为尿脓毒血症组,79例普通泌尿道感染患者对照组,检测２组患者PCT、超敏C反应蛋白（hypersensitive C-reactive protein,hs-CRP）、白细胞（white blood cells,WBC）及中性粒细胞水平;绘制受试者工作特征（receiver operating characteristic,ROC）曲线,评价PCT及相关炎症指标鉴别尿脓毒血症高危患者的能力,并对PCT与hs-CRP、WBC及中性粒细胞进行双变量相关性分析。结果：与对照组比较,尿脓毒症组PCT浓度（μg/L）、hs-CRP（mg/L）、WBC（×109个/L）及中性粒细胞（×109个/L）均明显增高（PCT浓度：3.113±1.953 vs. 0.057±0.0550;hs-CRP：18.820±15.460 vs. 7.440±14.520;WBC：12.860±4.002 vs. 8.150±4.630;中性粒细胞：90.335±3.975 vs. 74.30±15.40）,差异均有统计学意义（P＜0.05）。ROC曲线分析显示,PCT的曲线下面积（area under the curve,AUC）为1,高于hs-CRP、WBC、中性粒细胞（AUC分别为0.813、0.875、0.984）;且PCT为2.000 μg/L时,敏感性为90.2％,特异性为100％,优于其他传统炎症指标。双变量相关性分析显示,PCT与WBC和中性粒细胞数均呈显著正相关,分别为（rs=0.609,P=0.000;rs=0.057,P=0.000）,而与hs-CRP有相关性（rs=0.213,P=0.020）。结论：PCT在诊断尿脓毒血症具有较高的敏感度和特异度,有助于尿脓毒血症的早期诊断及早期治疗。     

IL-6、PCT、hs-CRP和WBC在新生儿细菌感染性疾病早期诊断中的价值

目的 深入探讨白细胞介素-6(IL-6)、血清降钙素原(PCT)、超敏C反应蛋白(hs-CRP)和白细胞计数(WBC)在新生儿细菌感染性疾病早期诊断中的价值,为临床采取及时有效的治疗措施提供科学依据.方法 选取2018年6月—2018年10月在山东第一医科大学第二附属医院新生儿科住院诊断为细菌感染性疾病的69例新生儿作...     

白细胞介素6与早产诊断

宫内感染是引起早产的重要因素之一。近年的研究发现白细胞介素6（IL－6）与早产关系密切。羊水中IL－6作为一项敏感的生物指标可预测亚临床感染性早产，高水平羊水IL－6预示保胎失败；宫颈分泌物IL－6浓度的测定，可以非侵袭性预测内膜感染性早产，从而及时给予治疗。血清IL－6浓度升高，是预测早产和感染的一个可靠标志，并具有非侵入性和可重复性等优点。