共查询到20条相似文献,搜索用时 31 毫秒
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Ethnopharmacological relevance
The caterpillar fungus Cordyceps militaris (CM; Clavicipitaceae) is a well-known traditional Chinese medicine that can be artificially cultivated on a large scale. We have previously demonstrated that its stimulatory action on ion transport in human airway epithelia is similar to Cordyceps sinensis (Clavicipitaceae), which has been traditionally used to treat respiratory diseases.Aim of the study
To investigate the signal transduction mechanism(s) underlying CM-induced ion transport activity in cultured human bronchial epithelia.Materials and methods
16HBE14o-, a human bronchial epithelial cell line, was used to study the regulation of ion transport by the water extract of CM. CM extract was added to the apical or basolateral aspect of the epithelia. In subsequent experiments, different Cl− channel and K+ channel blockers, adenylate cyclase and protein kinase A (PKA) inhibitors, and an intracellular Ca2+ chelator were used to examine the involvement of apical Cl− and basolateral K+ channels in mediating CM-induced Cl− secretion and the underlying signal transduction mechanism(s). PKA activity was also measured in 16HBE14o- cells.Results
CM stimulated Cl− secretion across 16HBE14o- monolayers in a dose-dependent manner. Cl− secretion could be inhibited by apical application of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl−channel blocker and the calcium-activated Cl− channel (CaCC) blocker. Cl− secretion was sensitive to basolateral application of different K+ channel blockers. Similar inhibitory patterns were obtained in nystatin-permeabilized epithelia. The CM-induced Cl− secretion could be inhibited by adenylate cyclase and PKA inhibitors as well as an intracellular Ca2+ chelator. Data from the PKA assay suggested that CM extract caused a significant increase in PKA activity compared with untreated control epithelia.Conclusions
These results suggest that CM extract stimulated Cl− secretion across human bronchial epithelia, possibly via apical CFTR and CaCC, and the basolateral K+ channels are involved in driving apical Cl− exit. The underlying signal transduction mechanisms involve both cAMP- and Ca2+-dependent pathways. 相似文献4.
Cordyceps sinensis extracts do not prevent Fas-receptor and hydrogen peroxide-induced T-cell apoptosis 总被引:2,自引:0,他引:2
Aqueous and alcohol extracts of Cordyceps sinensis (Berk) Succ. are used as a traditional medicine in China for the treatment of a wide range of diseases and are reported to have antioxidant and antiapoptotic properties. We therefore examined the ability of aqueous, organic, and alcohol extracts of Cordyceps sinensis to inhibit apoptosis induced either by hydrogen peroxide or Fas-receptor ligation; both stimuli induce apoptosis dependent on reactive oxygen species. Cells pre-incubated with Cordyceps sinensis extracts were equally sensitive to hydrogen peroxide and Fas-mediated apoptosis. Thus, the putative antioxidant and antiapoptotic properties of Cordyceps sinensis are insufficient to rescue cells from apoptosis induced by these stimuli in vitro. 相似文献
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Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris 总被引:10,自引:0,他引:10
This study aimed to elucidate pharmacological activities of Cordyceps militaris. The 70% ethanolic extracts of cultured mycelia (CME) and fruiting bodies (FBE) of Cordyceps militaris were prepared. CME was able to directly scavenge the stable free radical diphenyl-2-picrylhydrazyl (DPPH), indicating its antioxidant activity. Both CME and FBE showed topical anti-inflammatory activity in the croton oil-induced ear edema in mice. CME was found to contain acute anti-inflammatory activity, which was evaluated using the carrageenin-induced edema, and also strong antinociceptive activity in writhing test. CME and FBE contain potent inhibitory activity on the chick embryo chorioallantoic membrane (CAM) angiogenesis in a dose-dependent manner. Cordycepin, a metabolite of Cordyceps militaris, appeared to be at least partly responsible for its anti-inflammatory and anti-angiogenic activities. CME concentration-dependently inhibited the NO production and iNOS expression upon stimulation by lipoposaccharide in RAW 264.7, a murine macrophage cell line. In brief, we demontrate that Cordyceps militaris possesses anti-inflammatory and antinociceptive activites, and related antioxidant, anti-angiogenic, and NO production-inhibitory activities. 相似文献
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冬虫夏草及其混淆品亚香棒虫草的鉴别 总被引:2,自引:0,他引:2
殷仁亭 《现代中药研究与实践》2000,(2)
目的 :比较鉴别冬虫夏草与亚香棒虫草。方法 :对冬虫夏草、亚香棒虫草的外观性状、显微鉴别、紫外吸收、纸层析等进行了鉴别研究。结果 :冬虫夏草与亚香棒虫草的外观性状、显微鉴别、紫外吸收有明显差异 ,纸层析没有差异。结论 :亚香棒虫草不能混充冬虫夏草入药 ,应予以鉴别 相似文献
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Antioxidant activity of the extracts from fruiting bodies of cultured Cordyceps sinensis 总被引:6,自引:0,他引:6
Yamaguchi Y Kagota S Nakamura K Shinozuka K Kunitomo M 《Phytotherapy research : PTR》2000,14(8):647-649
Cordyceps sinensis is one of the most valued herbs in traditional Chinese medicine. We investigated the antioxidant activities of the cultured fruiting bodies of Cordyceps sinesis. The water and ethanol extracts of Cordyceps sinensis were found to possess a potent antioxidant activity. The scavenging effects of the extracts on superoxide were very weak, but the extracts moderately inhibited malondialdehyde formation via hydroxyl radical induced by SIN-1, a peroxynitrite generator. Of the extracts examined, the hot water extract (70 degrees C for 5 min) showed the greatest oxygen free radical scavenging activity. Also, when low-density lipoprotein (LDL) was incubated with macrophages in the presence of CuCl2 (1 microM), the hot water extract showed a strong inhibitory effect against lipid peroxidation in the medium and consequent accumulation of cholesteryl ester in macrophages. Their activities were comparable to that of authentic Cu/Zn SOD. These results suggest that the extracts of cultured Cordyceps sinensis possess potent antioxidant and anti-lipid peroxidation activities and inhibit accumulation of cholesteryl ester in macrophages via suppression of LDL oxidation. 相似文献
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The traditional Chinese medicine Cordyceps sinensis and its effects on apoptotic homeostasis 总被引:10,自引:0,他引:10
Cordyceps sinensis is a medicinal fungus of Traditional Chinese Medicine. While there are a wide range of reported uses of Cordyceps sinensis in the literature, the reports that extracts of this fungus may alter apoptotic homeostasis are most intriguing. However, there are significant challenges regarding research surrounding Cordyceps sinensis, such as the difficulty identifying the various species of Cordyceps and the many conflicting reports of pharmacological function in the literature. In this review we outline what is known about the ability of Cordyceps sinensis to alter apoptotic homeostasis, attempt to reconcile the differences in reported function, identify the challenges surrounding future Cordyceps sinensis research, and delineate options for overcoming these critical hurdles. 相似文献
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目的:明确工业化培育北虫草的主要生物活性成分在不同生长时期含量变化的规律.方法:采用HPLC和苯酚硫酸法,分别对不同生长时期的工业化培育的北虫草的虫草素、腺苷和虫草多糖含量的变化进行了跟踪.结果:培养周期内虫草素含量随生长期增长而不断增高,而腺苷和虫草多糖含量均在第40d达到最高值.结论:本研究结果对工业化生产,合理有... 相似文献
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目的:对北虫草菌株进行复壮处理以提高菌种活力。方法:用一株人工培育的北虫草菌株P感染刚化蛹的活体柞蚕蛹,蛹体近头部长出子实体后,待子实体近成熟期时采集孢子进行有性繁殖对原菌株进行复壮。结果:复壮后菌株10-2的子实体产量和生物转化率分别是原来菌株P的1.2倍和1.5倍。结论:在表面消毒过的活体柞蚕蛹上接种液体菌株活化复壮菌种的方法是可行的。 相似文献
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冬虫夏草是中国的一种传统名贵中药,富含了虫草多糖、虫草素、虫草酸、虫草多肽、麦角甾醇等多种单体活性成分。现代医学发现其具有抗肿瘤、免疫调节、降血糖、抗氧化、对肾脏的保护作用等广泛的药理学功能。目前的研究已证实冬虫夏草及其单体活性成分可以有效的治疗多种疾病。本文在检索近几年来有关冬虫夏草及单体活性成分药理作用的相关研究资料的基础上,主要从冬虫夏草的单体活性成分的研究现状及冬虫夏草的药理学作用的研究进展两个方面对目前冬虫夏草及单体活性成分药理作用进行归纳和总结,为今后进一步开发和利用冬虫夏草提供帮助。 相似文献
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人工蛹虫草子实体化学成分研究 总被引:6,自引:2,他引:6
目的:研究人工蛹虫草子实体的化学成分。方法:大孔吸附树脂和硅胶色谱柱分离纯化,根据理化性质和波谱数据鉴定结构。结果:分离鉴定了9个化合物,分别为:麦角甾-4,6,8(14)-四烯-3-酮(1),柠檬甾二烯醇(2),二十四烷酸单甘油酯(3),麦角甾醇(4),麦角甾醇过氧化物(5),麦角甾-7,22-二烯-3β,5α,6β-三醇(6),虫草素(7),腺苷(8),N-(2-羟乙基)腺苷(9)。结论:化合物1~3,6,9均为首次从该种真菌子实体中分离得到。 相似文献
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The establishment of a quantitative determination method by HPLC for adenosine and 3'-deoxyadenosine in Cordyceps militaris is reported. The sample was extracted with water under ultrasonic management for 2 hours. Chromatographic conditions: YWG C18 10 microns 5 mm x 200 mm; mobile phase consisted of NaH2PO4-NaOH buffer solution (pH 6.8); UV detection at 260 nm. The recoveries of adenosine and 3'-deoxyadenosine were 95.8% +/- 0.64% and 100.6% +/- 0.65% (n = 5) respectively. 相似文献
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人工虫草及野生虫草的成分比较研究 总被引:2,自引:0,他引:2
目的比较野生虫草与人工虫草中主要成分的差异。方法用比色法检测野生虫草与人工虫草中虫草酸及虫草多糖的含量,HPLC法比较野生虫草与人工虫草中的差异。结果野生虫草中虫草酸及虫草多糖的含量分别为11.4%和2.359%,人工虫草中虫草酸及虫草多糖含量分别为7.60%和3.13%,两种虫草HPLC图谱不完全相同。结论野生虫草与人工虫草成分不完全相同,虫草酸及多糖的含量也有差异。 相似文献
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冬虫夏草对系统性红斑狼疮模型大鼠的治疗作用 总被引:7,自引:0,他引:7
目的:观察冬虫夏草对系统性红斑狼疮模型大鼠的作用,为临床上系统性红斑狼疮的治疗开拓新的药物治疗提供理论基础。方法:12只MRL 1pr/1pr大鼠经处理后行淋巴结炎和蛋白尿的评价、肾功能的测定和血浆抗体分析。结果:冬虫夏草能够抑制系统性红斑狼疮大鼠淋巴结增生、降低蛋白尿以及抗ds-DNA抗体的水平和改善肾功能。结论:冬虫夏草对系统性红斑狼疮大鼠有一定的治疗作用,但其机理尚需进一步研究。 相似文献