深裂锈毛莓氯仿部位的化学成分研究

目的 研究深裂锈毛莓氯仿部位的化学成分.方法 采用硅胶柱色谱和重结晶方法进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构.结果 分离得到5个化合物,分别鉴定为2α,3β,19α-三羟基乌苏-12-烯-23-羧酸-28-O-β-D-吡喃葡萄糖酯(1)、2α,3β,19α-三羟基乌苏-12-烯-28-酸(2)、2α,3β,19α-三羟基乌苏-12-烯-23,28-二酸(3)、pseudosanguidiogenin A(4)、β-谷甾醇(5).结论 所有化合物均为首次从该植物中分离得到.     

黄荆子三萜类化学成分研究

目的 研究马鞭草科牡荆属植物黄荆果实的三萜类化学成分.方法 采用硅胶柱层析、ODS柱层析、Sephadex LH-20柱层析等色谱法分离纯化黄荆子三萜类化学成分,并根据理化性质和波谱数据对化合物结构进行鉴定.结果 分离得到了7个化合物,分别是2α,3α,19α-三羟基乌苏-12-烯-28-酸(1)、2α,3β-二羟基齐墩果-12-烯-28-酸(2)、2α,3β,19α-三羟基乌苏-12-烯-28-酸(3)、2α,3ββ,19α,23-四羟基齐墩果-12-烯-28-酸(4)、2α,3β,23-三羟基齐墩果-12-烯-28-酸(5)、2α,3α,24-齐墩果三羟基-12-烯-28-酸(6)、乌苏酸(7).结论 化合物1、3、4、5、6为首次从该属植物中分离得到.     

金樱子果实中三萜类成分的分离与鉴定

目的对金樱子中的三萜类化学成分进行研究。方法采用制备高效液相色谱、重结晶等分离纯化方法对金樱子果实的乙醇提取物进行成分分离纯化,利用1H-NMR、13C-NMR、ESI-MS波谱法对分离得到的化合物进行结构鉴定。结果从金樱子中得到12个三萜化合物,分别鉴定为1α,2α,3β,19α-四羟基-12-烯-28-乌苏酸(1α,2α,3β,19α-tetrahydroxyurs-12-en-28-oic acid,1)、2α,3α,19α,23-四羟基-12-烯-28-乌苏酸(2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid,2)、2α,3α,19α-三羟基-12-烯-28-乌苏酸(2α,3α,19α-trihydroxyurs-12-en-28-oic acid,3)、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸(2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid,4)、19α-羟基亚细亚酸-28-O-β-D-吡喃葡萄糖苷(19α-hydroxyasiatic acid-28-O-β-D-glucopyrannoside,5)、白桦酯酸(betulinic acid,6)、2α,3β-二羟基羽扇-20-烯-28-酸(2α,3β-dihydroxylup-20-en-28-oic acid,7)、2α,3β-二羟基羽扇-20-烯-28-酸甲酯(2α,3β-dihydroxylup-20-en-28-acid methyl ester,8)、3-O-反-对-香豆酰基麦珠子酸(3-O-trans-p-coumaroyl alphitolic acid,9)、3-O-顺-对-香豆酰基麦珠子酸(3-O-cis-p-coumaroyl alphitolic acid,10)、3-O-反-对-香豆酰基马斯里酸(3-O-trans-p-coumaroyl maslinic acid,11)和3-O-顺-对-香豆酰基马斯里酸(3-O-cis-p-coumaroyl maslinic acid,12)。结论化合物8和9为首次从蔷薇科中分离得到,化合物10¹2为首次从植物金樱子中分离得到。     

蛇毒中鞣花酸类及三萜类成分研究

自蛇毒(Duchesnea indica Fock)全草中分离出6个化合物,其中,2个为新化合物,分别命名为蛇莓甙A(duchesideA)和蛇莓甙B(duchesideB),经化学及光谱(UV,1R,¹HNMR,¹³CNMR,NOEDS和MS)分析确定,蛇莓甙A的结构为3'-O-甲基-鞣花酸-4-O-β-D-吡喃木糖甙(Ⅰ),蛇莓甙B的结构为3'-O-甲基-鞣花酸-4-O-α-L-呋喃阿拉伯糖甙(Ⅱ)。另外4个化合物为已知三萜类化合物:3β-羟基-乌苏烷-12-烯-28-羧酸(Ⅲ),2α,3β,19α-三羟基-乌苏烷-12-烯-28-羧酸(Ⅳ),2α,3β,19α-三羟基-乌苏烷-12-烯-28-羧酸-28-O-β-D-吡喃葡萄糖甙(Ⅴ),2α,3α,19α-三羟基-乌苏烷-12-烯-28-羧酸-28-O-β-D-吡喃葡萄糖甙(Ⅵ)。其中化合物Ⅳ,Ⅴ和Ⅵ为首次从该属植物中分离得到。     

夏枯草化学成分研究

采用柱色谱分离技术,从夏枯草全草70%乙醇浸膏的乙酸乙酯和正丁醇部位中分离得到14个化合物,经MS和NMR分析鉴定为:白桦脂酸(1)、乌苏酸(2)、2α,3α-二羟基-12-烯-28-乌苏酸(3)、2α,3β-二羟基-12-烯-28-乌苏酸(4)、2α,3β,24-三羟基-12-烯-28-乌苏酸(5)、2α,3α,19-三羟基-12-烯-28-乌苏酸(6)、齐墩果酸(7)、槲皮素-3-O-β-D-葡萄糖苷(8)、槲皮素-3-O-β-D-半乳糖苷(9)、山奈酚-3-O-β-D-葡萄糖苷(10)、芦丁(11)、丹参素甲酯(12)、丹参素乙酯(13)、迷迭香酸乙酯(14).其中化合物5、6、10、13为首次从该植物中分离得到,化合物5、13为首次从该属植物中分离得到.     

裸花紫珠的化学成分研究

目的研究裸花紫珠的化学成分。方法应用各种柱层析方法分离纯化,运用光谱数据解析鉴定化合物结构。结果分离鉴定了11个化合物,分别为：2α,3α,24-三羟基-齐墩果烷-12-烯-28-酸（Ⅰ）,2α,3β,24-三羟基-齐墩果烷-12-烯-28-酸（Ⅱ）,2α,3β,19α-三羟基-齐墩果烷-12-烯-28-O-β-D-葡萄糖苷（Ⅲ）,2α,3α,19α,23-四羟基-齐墩果烷-12-烯-28-O-β-D-葡萄糖苷（Ⅳ）,岳桦素（Ⅴ）,芹菜素（Ⅵ）,木犀草素（Ⅶ）,无梗五加苷B（Ⅷ）,aurantiamide acetate（Ⅸ）,β-谷甾醇（Ⅹ）,β-胡萝卜苷（Ⅺ）。结论化合物Ⅱ～Ⅳ,Ⅷ和Ⅸ为首次从紫珠属植物中分离得到,化合物Ⅰ和Ⅴ为从裸花紫珠中首次分得。     

雷公藤根化学成分的分离与鉴定

目的研究雷公藤(Tripterygium wilfordiiHook.f.)根的化学成分。方法采用硅胶柱色谱、Sephadex LH-20、开放ODS柱色谱、HPLC等各种色谱技术对雷公藤根的化学成分进行分离纯化;根据理化常数测定和光谱分析对分离得到的化合物进行结构鉴定。结果从雷公藤根乙醇提取物中分离得到7个化合物,分别鉴定为2α,3α,23-三羟基乌苏烷-12-烯-28-酸(2α,3α,23-trihydroxyurs-12-en-28-oic acid,1)、2α,3α,23-三羟基乌苏烷-12,20(30)-二烯-28-酸(2α,3α,23-trihydroxyurs-12,20(30)-dien-28-oic acid,2)、2α,3α,24-三羟基乌苏烷-12-烯-28-酸(2α,3α,24-trihydroxyurs-12-en-28-oic acid,3)、2α,3α,19α-三羟基乌苏烷-12-烯-28-酸(euscaphic acid,4)、熊果酸(urasolic acid,5)、齐墩果酸(oleanolic acid,6)、2S,2′S-aurantiamide acetate(7)。结论化合物1、2、3、7为首次从雷公藤属植物中分离得到。     

金樱叶的化学成分及抗炎活性研究

目的 对金樱子Rosa laevigata Michx.叶的化学成分及其抗炎活性进行研究,为进一步开发其药用潜能提供科学依据。方法 用大孔树脂色谱、ODS色谱及高效液相色谱法对金樱叶乙醇提取物进行分离纯化,根据理化性质和波谱数据对所得化合物进行结构鉴定,用荧光素酶活性检测法和ELISA法对部分化合物进行体外抗炎活性检测。结果 分离得到9个化合物,分别鉴定为：isopinfaenoic acid-28-O-β-D-glucopyranoside (1);2α,3β,23-三羟基乌苏-12,19-二烯-28-O-β-D-吡喃葡萄糖苷(2);2α,3β,19α-三羟基乌苏-12-烯-28-O-β-D-吡喃葡萄糖苷(3);苦莓苷F1 (4);2α,3β,23-三羟基乌苏-12,18-二烯-28-O-β-D-吡喃葡萄糖苷(5);5,7,3′,4′-四羟基-4′′-O-甲基-3-O-β-D-葡萄糖黄酮苷(6);芦丁(7);槲皮苷(8);异落叶松脂素-9-O-β-D-吡喃葡萄糖苷(9)。用脂多糖(LPS)刺激的NF-κB-293和RAW264.7W 2种炎症细胞模型筛选出具有不同程度抗炎活性的化合物...     

毛萼梅根化学成分研究

目的　研究毛萼梅根部的化学成分。方法　将MeOH提取物分散于水中,经EtOAc萃取后用柱色谱分离纯化,通过波谱方法鉴定化合物。结果　鉴定了7个化合物:2α,3β-二羟基乌苏-12,19-二烯-23,28-二酸(I),2α,3β,23-三羟基乌苏-12,18-二烯-28-酸(IIa),2α,3β,23-三羟基乌苏-12,19-二烯-28-酸(IIb),2α,3α-二羟基乌苏-12,18-二烯-28-酸(IIIa),2α,3α-二羟基乌苏-12,19-二烯-28-酸(IIIb),及化合物IIIa，IIIb的C-2,C-3位缩酮IVa和IVb。结论　I是1个新三萜酸。     

獐牙菜三萜化学成分研究

目的研究獐牙菜Swertia bimaculata的化学成分。方法采用硅胶柱、Sephadex LH-20柱色谱分离纯化,通过理化常数和光谱分析鉴定化合物的结构。结果从獐牙菜分离得到6种化合物,根据波谱分析和理化数据鉴定为：齐墩果酸（oeanlic acid,1）,乌苏酸（ursolic acid,2）,α-香树脂醇乙酸酯（α-amyrin acetate,3）,β-香树脂酮（β-amyrenone,4）,1β,3β-二羟基乌苏烷-12-烯-28-酸（1β,3β-dihydroxyurs-12-en-28-oicacid,5）,2α,3β,19α,23-四羟基-12-烯-28-乌苏酸（2α,3β,19α,23β-tetrahydroxyurs-12-en-28-oic acid,6）。结论化合物4～6为首次从獐牙菜属中分离得到,3为首次从獐牙菜中分离得到。     

Four new triterpenoids from the leaves of Psidium guajava

Four new triterpenoids, psiguanins A-D (1-4), along with 13 known ones (5-17), were isolated from the leaves of Psidium guajava. The structures of new compounds were determined as 2α,3β-dihydroxy-taraxer-20-en-28-oic acid (1), 2α,3β,12α,13β-tetrahydroxy-urs-28-oic acid (2), 2α,3β,12β,13β-tetrahydroxy-urs-28-oic acid (3), and 2α,3β,12β,13α-tetrahydroxy-urs-28-oic acid (4), respectively, on the basis of comprehensive spectroscopic methods and molecular modeling calculation. Among them, compound 4 was characterized as an unusual ursane-type triterpenoid with cis-fused C/D ring system.     

Two new triterpenoids from Callicarpa kwangtungensis

Two new triterpenoids, 2α,3β,16α,19α,23-pentahydroxyolean-12-en-28-oic acid (1) and 2α,3α,11α,21α,23-pentahydroxyurs-12-en-28-oic acid (2), were isolated from the aerial parts of Callicarpa kwangtungensis. Their structures were elucidated by 1D and 2D analyses, as well as MS and IR spectra.     

New ursane-type triterpene with NO production suppressing activity from Nauclea officinalis

Two new ursane-type triterpenes, named as 3β,?19α,?23,?24-tetrahydroxyurs-12-en-28-oic acid (1) and 2β,?3β,?19α,?24-tetrahydroxyurs-12-en-28-oic acid (2), together with two known triterpenoids, 3-oxo-urs-12-ene-27, 28-dioic acid (3) and quinovic acid-3-β-rhamnopyranoside (4), were isolated from the stems (with barks) of Nauclea officinalis. The structures of 1 and 2 were determined by the combined use of single-crystal X-ray diffraction and spectroscopic data analysis. The inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide in mouse macrophage RAW 264.7 cells were examined, and compound 1 was found to inhibit NO production, with the IC(50) value of 4.8?μM.     

Two new triterpenoid glycosides from the leaves of Anthocephalus chinensis

Nine compounds were isolated from the leaves of Anthocephalus chinensis by column chromatography on silica gel and Sephadex LH-20, and their structures were elucidated by spectroscopic techniques as clethric acid-28-O-β-d-glucopyranosyl ester (1), mussaendoside T (2), β-stigmasterol (3), hederagenin (4), ursolic acid (5), clethric acid (6), 3β,6β,19α,24-tetrahydroxyurs-12-en-28-oic acid (7), mussaendoside I (8), and cadambine (9). Compounds 1 and 2, and 7 and 8 were isolated from the plants of this genus for the first time, and compounds 1 and 2 were new triterpenoid glycosides.     

New terpenes from Salvia palaestina Benth. and Salvia syriaca L. growing wild in Jordan

The novel seco-ursane-type triterpenoid 3β,11α-dihydroxy-17,22-seco-17(28), 12-ursadien-22-oic acid (1) was isolated for the first time from a natural source from two Salvia species growing wild in Jordan, Salvia palaestina Benth. and Salvia syrica L. In addition to compound 1, S. syriaca afforded a new sesquiterpene named syriacine (2). S. palaestina also afforded 15 other known compounds, 6 of which are isolated for the first time from the plant, and these include velutin, hyptadienic acid, cirsilineol, 2α,3β-dihydroxyurs-12-en-28-oic acid, 2α,3α-dihydroxy-24-nor-4(23),12-oleanan-28-oic acid, and 2α,3β,24-trihydroxyurs-12-en-28-oic acid. S. syriaca also afforded 16 other known compounds, 7 of which are isolated for the first time from the plant. These are 1α,3α-dihydroxyolean-9(11),12-diene, maslinic acid, 2α,3β,24-trihydroxyolean-12-en-28-oic acid, 11-oxo-oleanolic acid, 11-oxo-ursolic acid, poriferast-5-en-3,7-diol, and pectolinangenin.     

黄腊果叶中化学成分的分离与鉴定(Ⅰ)

目的研究黄腊果(Stauntonia brachyanthera Hand.-Mazz.)叶中的化学成分。方法采用反复硅胶柱色谱法、Sephadex LH-20柱色谱法、制备薄层色谱法和重结晶等方法进行分离纯化,通过理化性质与波谱分析方法鉴定化合物的结构。结果从黄腊果叶体积分数70%乙醇溶液提取物中分离鉴定6个化合物,分别为3β,20α,24-trihydroxy-29-norolean-12-en-28-oic acid 24-O-β-D-glucopyrano-side(1)、牡荆素(vitexin,2)、山奈酚(kaempferol,3)、apigenin-7-O-β-D-neospheroside(4)、3,23-di-hydroxy-akebonic acid(5)、3-O-β-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl-30-norolean-12,20(29)-dien-28-oic acid(6)。结论化合物1～4为首次从野木瓜属植物中分离得到,化合物5为首次从黄腊果中分离得到。     

Die Triterpensäuren aus Coleus amboinicus Loureiro

Triterpenic Acids from Coleus Amboinicus Loureiro Eight triterpenic acids have been isolated from the leaves of the South-American lamiaceae Coleus amboinicus Loureiro. 2α,3α-Dihydroxyolean-12-en-28-oic acid, 2α,3α,19α-trihydroxyurs-12-en-28-oic acid, and 2α,3α,19α-trihydroxyurs-12-en-28-oic acid were found for the first time in lamiaceae and 2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid was found the first time in plants.     

Cytotoxic triterpenoids from Berberis koreana

A bioassay-guided fractionation and chemical investigation of the trunk of Berberis koreana led to the isolation and identification of three new triterpenoids, 2 α,3 α,19 α-trihydroxy-urs-12-en-24-formyl-28-oic acid (1), 2 α,3 β,21 α-trihydroxy-urs-12-en-28-oic acid ( 2), and 3 β-acetyloxy-1-oxo-olean-12-en-28-oic acid (3), along with seven known triterpenoids (4-10). The structures of these new compounds were determined through spectral analysis, including extensive 2D-NMR data. The new compounds 1-3 showed significant cytotoxicity against the A549, SK-OV-3, SK-MEL-2, and HCT-15 human tumor cell lines with IC(50) values ranging from 7.17 to 48.73?μM.