齐墩果酸抑制3T3-L1脂肪细胞脂质堆积

目的：从57个中药化合物筛选具有抑制3T3-L1脂肪细胞脂质堆积的活性成分。方法：3T3-L1细胞培养至融合后用化合物进行干预,并诱导使其分化,采用高内涵影像方法检测细胞中脂滴含量。对发现的活性成分用ToxInsight体外毒性检测方法评价其肝毒性。结果：发现并验证活性成分齐墩果酸（OA）具有显著的脂质形成抑制作用,其半数抑制浓度（IC50）为14.5 μmol·L-1,且在60 μmol·L-1测试浓度范围内对HepG2 细胞无显著损伤作用。结论：OA 具有显著的降低脂质形成作用,是潜在的降脂候选化合物。     

齐墩果酸对3T3-L1脂肪细胞分化及糖脂代谢的影响

目的:观察齐墩果酸(oleanolic acid,OA)对脂肪细胞分化和糖脂代谢的影响并探讨其机制。方法:在诱导脂肪分化时用OA干预,油红O染色后,通过比色法分析脂肪细胞的分化程度。采用葡萄糖氧化酶法检测OA对脂肪细胞葡萄糖消耗的影响;采用酶联免疫吸附法检测上清液中的游离脂肪酸浓度;采用RT-PCR法检测脂肪细胞PPAR-γ和GLUT-4 mRNA的表达。结果:与溶媒对照组比较,OA能显著增加脂肪细胞葡萄糖消耗量(P<0.05或P<0.01),在1~30μmol/L浓度范围时,其作用随着剂量增加显著增强;OA能促进前脂肪细胞的分化,在3~30μmol/L浓度范围内,OA组油红O染液光密度值均显著高于溶媒对照组(P<0.05或P<0.01)。同时,OA还能减少游离脂肪酸的产生,其30μmol/L剂量时游离脂肪酸的含量只有溶媒对照组的47.2%,显著低于溶媒对照组(P<0.01)。与溶媒对照组比较,30μmol/L OA能显著增加脂肪细胞PPAR-γ及GLUT-4 mRNA的表达(P<0.05)。结论:OA能促进脂肪细胞分化和葡萄糖的消耗,减少游离脂肪酸的产生,其机制可能与上调脂肪细胞PPAR-γ和GLUT-4 ...     

芦丁对3T3-L1前脂肪细胞分化的作用

目的探索芦丁对3T3-L1前脂肪细胞分化的影响。方法采用3T3-L1前脂肪细胞分化模型和油红O染色法,选用罗格列酮作为阳性对照药,观察芦丁对3T3-L1前脂肪细胞分化的作用,于510 nm波长下测定芦丁对脂含量的影响。利用流式细胞仪测定芦丁对分化的脂肪细胞吸收葡萄糖的影响。结果芦丁在0.5μmol/L和1μmol/L两个浓度下均能促进3T3-L1前脂肪细胞分化过程,提高脂含量,并促进了葡萄糖吸收。结论芦丁能够促进3T3-L1前脂肪细胞分化,并促进了分化的脂肪细胞吸收葡萄糖。     

柚皮苷对前脂肪细胞3T3-L1增殖和诱导分化的影响

目的观察柚皮苷对前脂肪细胞3T3-L1增殖和分化的影响,并探讨其影响3T3-L1分化的可能作用机制。方法培养3T3-L1细胞,并用不同浓度的柚皮苷进行干预,以四甲基偶氮唑盐(MTT)法检测细胞增殖,用油红O染色和染色比色法分析脂肪细胞的分化程度。逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物酶增殖物激活受体γ2(PPARγ2)、CCAAT/增强子结合蛋白α(C/EBPα)mRNA的表达。结果柚皮苷能抑制3T3-L1前脂肪细胞的增殖和分化,并能抑制PPARγ2、C/EBPα基因的表达。结论柚皮苷可抑制3T3-L1前脂肪细胞的增殖,并能通过下调分化相关基因的表达抑制分化。     

杜仲叶提取物对3T3-L1前脂肪细胞分化的影响

研究了杜仲叶提取物对3T3-L1前脂肪细胞向脂肪细胞分化的影响。     

黄连解毒汤对3T3-L1前脂肪细胞分化的抑制

以3T3-L1前脂肪细胞探讨了黄连解毒汤及其组成生药对脂肪细胞分化的影响。     

雪菊不同提取部位对3T3-L1前脂肪细胞增殖与分化的影响

目的：以3T3-L1前脂肪细胞为载体,考察雪菊总提物、正丁醇部位、黄酮单体CB-1及CB-2对该细胞增殖与分化的影响。方法：雪菊4个不同提取部位分别设3个剂量组。MTT法检测雪菊4个不同提取部位对3T3-L1细胞增殖的作用,油红O染色并通过比色分析细胞分化过程中胞浆脂质的形成与积累。结果：与对照组相比,雪菊总提取物100 μg·mL-1,正丁醇部位0.5、5、50 μg·mL-1,黄酮单体CB-1、CB-2在50 μg·mL-1浓度时对细胞增殖有显著抑制作用（P<0.01）;正丁醇部位的作用呈一定的量效关系（相关系数r=-0.903）。油红O染色结果表明,与对照组相比,雪菊总提取物1、10、100 μg·mL-1均可以显著抑制细胞分化,减少细胞中脂质的形成（P<0.01）;正丁醇部位能够剂量依赖性地抑制细胞分化（r=-0.779）;CB-1与CB-2在50 μg·mL-1浓度时对细胞分化有显著抑制作用（P<0.01）。结论：雪菊总提物、正丁醇部位、CB-1与CB-2均能够抑制3T3-L1前脂肪细胞的增殖与分化,减少细胞中脂质积累。     

三七总皂苷对3T3-L1细胞增殖分化与分泌功能的影响

目的 观察三七总皂苷对3T3-L1细胞增殖、分化和分泌Leptin、PAI-1的影响,探讨其调脂作用的可能机制。方法 培养3T3-L1细胞,并用不同浓度的三七总皂苷(5,50,100 μg·mL-1)进行干预,以四甲基偶氮唑盐(MTT)法检测细胞增殖;用油红O染色和染色比色法分析脂肪细胞的分化程度,用逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物酶增殖物激活受体γ2(PPARγ2)、CCAAT/增强子结合蛋白α(C/EBPα)mRNA的表达;用ELISA法检测细胞培养上清中Leptin、PAI-1的含量。结果 三七总皂苷低、中浓度组对模型细胞增殖无明显影响(P ＞ 0.05),高浓度组能促进细胞的增殖(P ＜ 0.05);中、高浓度组明显抑制模型细胞的分化,并能抑制PPARγ2、C/EBPα基因的表达(P ＜ 0.01);药物对模型细胞分泌Leptin、PAI-1有明显抑制作用(P ＜ 0.01)。结论 三七总皂苷可能通过抑制3T3-L1前脂肪细胞的分化和抑制其分泌因子而达到调脂作用,以中剂量作用效果最好。     

催产素对3T3-L1脂肪细胞糖脂代谢的影响

目的观察催产素对3T3-L1脂肪细胞糖脂代谢的影响。方法 3T3-L1前脂肪细胞体外培养,并诱导其分化成熟为脂肪细胞。研究催产素对脂肪细胞葡萄糖消耗量以及三酰甘油、游离脂肪酸和甘油的影响。采用实时荧光定量PCR法检测糖脂代谢相关基因GLUT-1、GLUT-4、ATGT、HSL的mRNA表达。结果与对照组比较,催产素20、50、100μg/m L组葡萄糖消耗量有所增加,且表现出剂量相关。催产素组较对照组的三酰甘油降低,而甘油和游离脂肪酸增高。催产素50μg/m L组中脂代谢相关基因HSL表达明显高于对照组,糖代谢相关基因GLUT-4 mRNA表达水平增加。结论催产素处理可减少3T3-L1细胞脂质合成、增加脂质分解作用,并可明显改善脂质积聚。     

3T3-L1前脂肪细胞与肥胖的相关性研究进展

肥胖已经成为危害人类健康的重要疾病,研究减肥药物的体外作用靶点和机制变得越来越重要。3T3-L1前脂肪细胞是目前研究脂肪代谢紊乱疾病最广泛的一种细胞系,已经被培养成为成熟的细胞系。本文旨在对3T3-L1前脂肪细胞的培养、鉴定及其在肥胖中研究的应用进行综述,为肥胖的体外研究提供思考方法。     

Effects of green tea catechin-induced lipolysis on cytosol glycerol content in differentiated 3T3-L1 cells

The influence of catechins in green tea on lipolysis in fully differentiated 3T3-L1 cells was studied and glycerol release into the buffer, cytosol and residual triglyceride were measured.The addition of (-)-epigallocatechin-3-gallate (EGCG) stimulated glycerol release into the cytosol significantly after incubation for 4 h, but had no effect on that into the buffer. However, (+)-catechins did not produce a significant increase in lipolysis. These data suggested that EGCG has strong lipolytic activity.     

葛根素对3T3-L1前脂肪细胞分化及胰岛素抵抗模型糖代谢的影响

目的:研究葛根素(Pur)对3T3-L1前脂肪细胞增殖、分化的影响,以及在胰岛素抵抗状态下对3T3-L1脂肪细胞葡萄糖代谢的影响。方法:不同浓度Pur干预3T3-L1细胞,MTT法检测其对细胞增殖的影响;油红O染色法检测其对前脂肪细胞分化过程及成脂的影响;地塞米松诱导3T3-L1细胞建立胰岛素抵抗模型,用不同浓度Pur进行干预,测定细胞的葡萄糖消耗量。结果:Pur(3～300μmol/L)对3T3-L1前脂肪细胞增殖无明显影响;Pur(30～300μmol/L)促进3T3-L1细胞的分化成脂,增加胰岛素抵抗状态下3T3-L1脂肪细胞的葡萄糖代谢,且具有量效关系。结论:Pur能够促进3T3-L1细胞的分化成脂,增加胰岛素抵抗状态下3T3-L1脂肪细胞的葡萄糖代谢,改善胰岛素抵抗。     

Stereospecific effects of catechin isomers on insulin induced lipogenesis in 3T3-L1 cells

We studied the influence of (+)- and (-)-catechin contained in green tea on insulin induced lipogenesis in 3T3-L1 cells. In 14 days of culture with insulin, the intracellular triglyceride concentration and the activities of glycerophosphate dehydrogenase, a marker of adipose conversion, increased. The addition of 0.02 mg/ml (+)-isomer stimulated the accumulation of triglyceride induced by insulin, but the addition of the same concentration of (-)-isomer inhibited lipogenesis. The activities of glycerophosphate dehydrogenase were changed by (+)- and (-)-catechin in the same direction as the corresponding changes in triglyceride accumulation. These data suggest a biological significance of catechins, with opposite effects on lipid metabolism depending on the isomer.     

Acceleration of lipid degradation by sericoside of Terminalia sericea roots in Fully differentiated 3T3-L1 cells

Sericoside is a traditional herbal saponin from Terminalia sericea (Family Combretaceae). The influence of sericoside on lipolysis was studied in fully differentiated 3T3-L1 cells, and glycerol release into the cytosol and residual triglyceride were measured. The addition of sericoside stimulated glycerol release into the cytosol from deposited triglyceride in a dose- and time-dependent manner. These data suggested that sericoside has a strong lipolytic activity.     

Effects of capsaicin on lipid catabolism in 3T3-L1 adipocytes

Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is a pungent ingredient of red peppers, and has been reported to reduce body weight gain and adiposity in rodents. The present study investigated the effects of capsaicin on lipid catabolism in differentiated 3T3-L1 adipocytes. Capsaicin decreased the intracellular lipid content in a concentration-dependent manner. The release of glycerol into the medium was increased by the addition of capsaicin. The mRNA levels of genes involved in lipid catabolism such as hormone sensitive lipase (HSL), carnitine palmitoyl transferase-Iα (CPTI-α) and uncoupling protein 2 (UCP2) were up-regulated significantly. These results suggest that capsaicin exerts its lipolytic action by increasing the hydrolysis of triacylglycerol in adipocytes, and that these effects are mediated at least partially by regulation of the expression of multiple genes that are involved in the lipid catabolic pathway, such as HSL and CPT-Iα, and those involved in thermogenesis such as UCP2.     

Salacia reticulata inhibits differentiation of 3T3-L1 adipocytes

Aim of the study

The purpose of this study was to define antidiabetic effects of fruit of Vaccinium arctostaphylos L. (Ericaceae) which is traditionally used in Iran for improving of health status of diabetic patients.

Materials and methods

Firstly, we examined the effect of ethanolic extract of Vaccinium arctostaphylos fruit on postprandial blood glucose (PBG) after 1, 3, 5, 8, and 24 h following a single dose administration of the extract to alloxan-diabetic male Wistar rats. Also oral glucose tolerance test was carried out. Secondly, PBG was measured at the end of 1, 2 and 3 weeks following 3 weeks daily administration of the extract. At the end of treatment period the pancreatic INS and cardiac GLUT-4 mRNA expression and also the changes in the plasma lipid profiles and antioxidant enzymes activities were assessed.Finally, we examined the inhibitory activity of the extract against rat intestinal α-glucosidase.

Results

The obtained results showed mild acute (18%) and also significant chronic (35%) decrease in the PBG, significant reduction in triglyceride (47%) and notable rising of the erythrocyte superoxide dismutase (57%), glutathione peroxidase (35%) and catalase (19%) activities due to treatment with the extract. Also we observed increased expression of GLUT-4 and INS genes in plant extract treated Wistar rats. Furthermore, in vitro studies displayed 47% and 56% inhibitory effects of the extract on activity of intestinal maltase and sucrase enzymes, respectively.

Conclusions

Findings of this study allow us to establish scientifically Vaccinium arctostaphylos fruit as a potent antidiabetic agent with antihyperglycemic, antioxidant and triglyceride lowering effects.     

3,4-Oxo-isopropylidene-shikimic acid promotes adiopkine expression during murine 3T3-L1 fibroblast differentiation into adipocytes

Objective3,4-Oxo-isopropylidene-shikimic acid (ISA), a derivative of shikimic acid, has exhibited ameliorative effect on cognitive impairment in experimental animal models of dementia. This study investigated the effect of ISA on lipid accumulation and adipokine secretion during differentiation of 3T3-L1 fibroblasts to adipocytes.Methods3T3-L1 cells were cultured and treated with ISA (50–800 μM) from days 3–8. Lipid accumulation and triglyceride content were measured. Gene expression of adipokines (adiponectin, leptin, and resistin), CCAAT/enhancer binding protein (C/EBP) β, C/EBP α and peroxisome proliferator-activated receptor γ (PPAR γ) and PPAR target genes, including adipocyte fatty acid binding protein (aP2) and fatty acid synthase (FAS) were investigated.ResultsISA promoted 3T3-L1 fibroblast differentiation to adipocytes and increased triglyceride content by 26%. On mechanistic levels, ISA increased expressions of C/EBP β, PPAR γ, C/EBP α, aP2 and FAS. Moreover, ISA stimulated expressions of adipokines secreted by adipocytes, including adiponectin, leptin, and resistin.ConclusionsThese findings demonstrated that ISA promoted adipogenesis by up-regulating expressions of C/EBP β, PPAR γ, C/EBP α, aP2 and FAS, and also stimulated adipokines during adipocyte differentiation. Further study should clarify the relationship between stimulation of adipokines and cognitive enhancing effect of ISA.     

Effect of mollugin on apoptosis and adipogenesis of 3T3-L1 preadipocytes

The effect of mollugin, isolated from the roots of Rubia cordifolia L., on cell viability, apoptosis and adipogenesis in 3T3‐L1 preadipocytes was investigated. The inhibitory effect of mollugin (40–60 µm ) on cell viability was more significant in differentiated adipocytes than in 3T3‐L1 preadipocytes. In 3T3‐L1 cells, the cytotoxicity of mollugin was accompanied by apoptotic events including mitochondrial membrane potential (Δψm) loss and activation of caspase‐9, ‐3 and ‐7, leading to PARP degradation. Although the presence of 20 µm mollugin during induced adipocytic differentiation of 3T3‐L1 cells for 6 days failed to affect the cell viability, it could almost completely abrogate the differentiation‐associated morphology change and intracellular lipid accumulation. A similar level of inhibition was observed, when 20 µm mollugin was present during the early stage (D0–D2) of the differentiation period. In addition, the expression of C/EBPα, PPARγ1 and PPARγ2 was significantly down‐regulated. The presence of 20 µm mollugin during either middle stage (D2–D4) or late stage (D4–D6) of the differentiation period, however, caused the inhibition to a lesser extent. These results indicated that mollugin at high concentrations (40–60 µm ) exerted cytotoxicity via inducing apoptosis, whereas mollugin at a low concentration (20 µm ) suppressed adipocytic differentiation without exerting cytotoxicity in 3T3‐L1 preadipocytes. Copyright © 2010 John Wiley & Sons, Ltd.     

淫羊藿苷对3T3-L1前脂肪细胞增殖及分化的影响

目的研究淫羊藿苷(ICA)不同作用浓度及不同作用时间对3T3-L1前脂肪细胞增殖和分化的抑制作用。方法采用MTT法观察ICA对小鼠3T3-L1前脂肪细胞增殖的影响;应用ICA诱导小鼠3T3-L1前脂肪细胞分化,油红O染色测定细胞内脂质含量,观察ICA对小鼠3T3-L1前脂肪细胞分化的影响。结果 67μg/m L ICA对细胞增殖起抑制作用,持续至96 h并达到最大;30μg/m L和15μg/m L ICA在72 h、96 h对细胞增殖起抑制作用。结论较高浓度ICA对3T3-L1前脂肪细胞的分化有抑制作用,而低浓度ICA对3T3-L1前脂肪细胞的分化作用不明显。