鼠伤寒沙门氏菌感染51例临床分析

本文报道51例鼠伤寒沙门氏菌感染病例,发现其发病率以6～9月份最高,占72.5%;男女之比为2:1;3岁以下者患病率最高,占98％.其主要临床表现为急性发热、腹泻、呕吐、脱水、酸中毒。文中还就其预防、防止交叉感染及治疗进行了讨论。     

鼠伤寒沙门氏菌感染4例

鼠伤寒沙门氏菌感染４例李金秀（福建厦门市集美医院）关键词鼠伤寒；沙门氏菌；感染近年来，随着世界多数地区伤寒发病率日趋下降，而非伤寒沙门氏菌感染却见增多，以鼠伤寒沙门氏菌感染（以下简称鼠伤寒）尤为显著。国内鼠伤寒的报道有所增加，儿科病例的特点是；发病年...     

婴幼儿鼠伤寒沙门氏菌感染132例

现将我科1986～1988年收治的132例婴幼儿鼠伤寒沙门氏菌感染报告如下: 男90例,女42例。年龄5天～1月29例,1月～1岁99例,1岁～2岁3例,3岁1例。院内感染79例,院外感染53例。     

新生儿鼠伤寒沙门氏菌败血症22例报告

<正> 鼠伤寒沙门氏菌感染是非伤寒沙门氏菌属感染之一。近年来国内外文献报道其发病率有逐渐上升的趋势.我科自1989年8月至1991年3月发现22例新生儿鼠伤寒沙门氏菌感染,经粪和血培养证实。现报道如下:     

新生儿室鼠伤寒沙门氏菌感染25例报告

新生儿室鼠伤寒沙门氏菌感染２５例报告吴光济陈琅池友肆陈骏杨陈发林杨珍春关键词鼠伤寒沙门氏菌病；暴发流行；新生儿室福建省立医院儿科（福州３５０００１）１９９１年７月我院产科新生儿室爆发鼠伤寒沙门氏菌感染（鼠伤寒），现报告如下。１临床资料１．１一般资料发...     

32例鼠伤寒沙门氏菌医院感染调查

包头铁路医院于１９９１年３月～５月误收鼠伤寒沙门氏菌肠炎患者，在儿科病房造成交叉感染，并发生３２例，男２３例，女９例，多数为婴幼儿，其中２岁以内的２６例，占８１．２５％，主要症状表现为发热，恶心呕吐，腹泻，大便多为黄绿色稀水样，便次在６～１２次／日不等，经流行病学调查，显示３２例患儿中８例为原发性感染，１例为在家接触感染，其余２３例均为住院期间交叉感染，对儿科医护人员手及备品进行采样８５份检查，检     

鼠伤寒沙门氏菌感染64例临床观察

鼠伤寒沙门氏菌感染６４例临床观察赵秀芝我国各地区伤寒、副伤寒发病率日趋减少，而鼠伤寒沙门氏菌感染却有增多趋势，原因是带菌者不易控制，患者病后长期排菌，单用抗生素效果不好，而且易产生耐药菌株而延长排菌期，带菌者除人类外，家禽、家畜等多种动物都有带菌，细...     

40例小儿鼠伤寒沙门菌临床分析

目的：对本地区鼠伤寒沙门菌感染患儿进行临床分析。方法：对我院2012年—2015年经粪便培养证实为鼠伤寒沙门菌感染患儿40例的临床表现、实验室检查、药敏及治疗和转归情况进行分析。结果：本组病例发病以婴幼儿多见,特别是6月-2岁多见;绝大部分病例出现腹泻及发热表现;全部病例炎症特异性指标并不能反应感染鼠伤寒沙门菌,或是提示感染了鼠伤寒沙门菌;药敏试验中亚胺培南敏感率 100%,哌拉西林／他唑巴坦敏感率85.74％、氨曲南敏感率78.57％,头孢他定敏感率为64.28％,对头孢曲松敏感率为57.14％,敏感度越高治疗效果越好。结论：粪便培养是确诊鼠伤寒肠炎的重要方法,结合药敏试验选用敏感抗生素可取得较好的治疗效果。     

Anti-angiogenesis Effect on Glioma of Attenuated Salmonella Typhimurium Vaccine Strain with flk-1 Gene

To investigate the anti-vasculature effects and the anti-glioma effects of attenuated Salmonella typhimurium vaccine strain expressing VEGFR2 (flk-1) gene, plasmid pcDNA3, 1-flk1 was constructed and electro-transfected into live attenuated Salmonella typhimurium strain SL7207. Mouse models of intracranial G1261 glioblastoma were treated with an orally administered attenuated Salmonella typhimurium expressing flk-1 gene. The survival period was recorded and vessel density was observed by immunofluorescence. CTLs activity was measured by MTT assay. Our results showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could significantly inhibit glioblastoma growth, reduce vessel density, prolong the survival period and improve the survival rate in these mice. The flk-1 specific CTLs activity was increased obviously after the vaccination. Our study showed that attenuated Salmonella typhlmurium vaccine strain expressing flk-1 gene could break peripheral immune tolerance a in glioma gainst this self-antigen and kill endothelial cells by the orally administered vaccine and can be used for both prophylactic and therapeutic purposes.     

Expression of Human Sperm Membrane Protein in the Recombinant Salmonella Typhimurium Vaccine

A 550 bp cDNA fragment of HSD-Ⅰ coding for an extracellular domain of hu-man sperm membrane protein(hSMP-1)was ligated with an Adapter containing the universal stop codon,and the ligated fragment cDNA was then cloned into the MAS of pUC19.The desired plasmid with correct open reading frame was obtained, and was cut with EcoR Ⅰ.The insert was purified and then cloned into the two asd^ Salmonella expression vectors(the low copy number plasmid-pYA292 and the high copy number plasmid-pYA3137).The recombinant plasmid containing the insert with the correct orientation was selected by restriction enzyme digestion analysis. The recom-binant plasmids were transferred into the non-pathogenic Salmonella typhimurium X4550,which was deletion of the △cya, △crp and △asd genes.Western-blot analysis of the whole cell lysate of the two recombinants of S.typhimurium showed a predomi-nant protein band at 21 KD,which reacted with the anti-hSMP-1 antiserum. The re-sult indicated that two recombinants of S.typhimurium containing the 550 bp cDNA of HSD-Ⅰ were constructed and the characteristics of their growth in vitro were deter-mined. They may be used as new potential mucosalanti fertility.     

头孢曲松钠治疗儿童鼠伤寒沙门氏菌肠炎疗效分析

目的探讨头孢曲松钠治疗儿童鼠伤寒沙门菌肠炎的临床疗效。方法总结该院2005年1月—2012年8月鼠伤寒沙门菌肠炎患儿86例,其中头孢曲松钠组60例,美洛西林钠/舒巴坦钠组26例,通过检查两组患儿的大便情况及常规检查,分析头孢曲松钠对儿童鼠伤寒沙门菌肠炎的疗效。结果头孢曲松钠组总有效率78.3%,美洛西林钠舒巴坦钠组有效率11.5%,两者疗效比较,差异有统计学意义(P<0.05)。结论头孢曲松钠治疗鼠伤寒沙门菌有效率高,耐药者少,治疗效果好。     

板蓝根多糖对宿主抵抗鼠伤寒沙门菌感染能力的影响

目的:观察板蓝根多糖注射鼠伤寒沙门菌感染的小鼠后,对小鼠抵抗鼠伤寒杆菌能力的影响。方法:水煮法提取板蓝根多糖,配成悬液,腹腔注射感染鼠伤寒的小鼠,观察小鼠的生存时间,脾脏的重量指数,脏器菌落计数,检测小鼠血清中抗体IgG水平,用ELISA法测定小鼠脾脏释放的细胞因子。结果:注射板蓝根多糖的小鼠,生存时间延长,脾脏的重量指数,菌落计数均比对照组明显降低,血清中抗体IgG水平升高,脾脏细胞分泌细胞因子IFN-γ水平上升。结论:板蓝根多糖能够通过增强抗体的产生,调节细胞因子的释放,增强宿主的体液免疫和细胞免疫功能,保护宿主抵抗胞内菌的感染。     

Characterization of Salmonella Enterica Serotype Typhimurium from Outpatients of 28 Hospitals in Henan Province in 2006

Objective To characterize the diarrheal patients with Salmonella typhimurium （S. typhimurium） infections and to set up the first baseline for S. typhimuri_um pulsed-field gel electrophoresis （PFGE） patterns in Henan province, thus laying a foundation for comprehensive surveillance of Salmonella in human as well as foods. Methods S. typhimurium isolates recovered from outpatients with diarrhea in Henan province from May to October of 2006 were characterized. Antimicrobial susceptibility tests of 8 antimicrobial agents and PFGE were carried out to analyze the S. typhimurium isolates. Results Twenty-four （0.9%） S. typhimurium isolates were identified from 2661 stool specimens of diarrheal cases. Eighty-eight percent of isolates showed resistance to at least one antimicrobial agent. The resistance to chloramphenicol （79%） was most common. Fifty-eight percent of isolates were resistant to ciprofloxacin. All the 14 ciprofloxacin-resistant isolates were resistant to more than five antimicrobial agents. Thirty-three percent of S. typhimurium isolates were resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline （R-type ACSSuT）. Eight antimicrobia-resistant phenotypes were found among the 24 isolates in 16 PFGE patterns. Conclusion The rate of multidrug-resistant S. typhimurium is relatively high in S. typhimurium PFGE patterns of Henan province. Multidrug-resistant S. typhimurium should be considered a public health threat.     

乙酰化对鼠伤寒沙门菌酸耐受应答的影响

目的研究乙酰化／去乙酰化酶Pat／CobB对沙门菌酸耐受应答的影响。方法利用λ-Red同源重组系统分别敲除鼠伤寒沙门菌乙酰化酶／去乙酰化酶编码基因pat和cobB。利用生长曲线比较生长差异;Real-TimePCR检测参与酸应激的部分基因转录变化;采用Cfu计数法检验敲除株和野生株在对数期和平台期酸耐受应答中的差异。结果成功构建了鼠伤寒沙门菌pat和cobb敲除株。pat和cobb敲除不影响细菌生长,也不影响对数期和平台期的鼠伤寒沙门菌对酸的耐受应答能力。结论鼠伤寒沙门菌中编码乙酰化／去乙酰化酶的基因pat/cobB突变不会影响菌的生长,也不参与酸耐受应答。     

儿童鼠伤寒沙门菌败血症24例临床分析

目的:探讨儿童鼠伤寒沙门菌败血症的临床特点及疗效。方法:对确诊为鼠伤寒沙门菌败血症的24例儿童患者进行回顾性分析,研究其临床表现及疗效。结果:婴幼儿多见,平均年龄9个月,主要表现为发热、消化道症状,外周血白细胞16例(66.7%)正常、2例(8.33%)减少,6例增高(25%),临床分离株对头孢他啶、头孢噻肟、头孢吡肟、左氧氟沙星、亚胺培南敏感率分别为83.3%、87.5%、91.7%、66.7%、100%,24例初治均采用头孢曲松钠,22例血培养正常,2例仍为阳性,根据药物敏感试验选用抗生素治疗后均治愈。结论:头孢三代耐药率相对较低,是治疗婴幼儿鼠伤寒沙门菌败血症的首选药物。     

变形链球菌表面蛋白pac基因A区片段在减毒鼠伤寒沙门氏菌的表达

【目的】检测携带变形链球菌表面蛋白 pac基因A区片段的重组质粒 pET 17b/A在减毒鼠伤寒沙门氏菌SL32 6 1的表达情况。【方法】应用SDS PAGE技术检测重组减毒鼠伤寒沙门氏菌SL32 6 1中是否有新的蛋白条带 ,Westernblot检测条带的抗原性。【结果】SDS PAGE显示重组减毒鼠伤寒沙门氏菌SL32 6 1中有 35ku的新蛋白条带 ,Westernblot显示在相应的位置上有特异的条带。【结论】重组减毒鼠伤寒沙门氏菌SL32 6 1表达的变形链球菌表面蛋白 pac基因A区片段具有抗原性。