Determination of CD_(30) Expression on Peripheral Blood T Lymphocyte Subsets in Patients with Hemorrhagic Fever with Renal Syndrome by FCM

Hantavirus can cause panphilic infection in hu-man beings and proliferate especially in such impor-tantimmune organs and cells,as liver,spleen,bonemarrow,lymph nodes,thymus,lymphocyte and soon,thusresulting in immunity disturbance.CD30 is atransmembrane glycoprotein,belonging to the TNF/NGF receptor superfamily.In recent years,the bio-logical significance of CD30 elicited greatconcern.Theclose relationship between the expression of CD30antigen and theactivation and function of T cellssug-…     

Clinical Observation on the Effect of Zengse Pill （增色丸） in Treating Patients with Vitiligo of Qi-stagnancy and Blood-stasis Syndrome Type

Objective： To observe the clinical efficacy of Zengse Pill （增色丸, ZSP） on patients with vitiligo of qi-stagnancy and blood-stasis syndrome type （V-QB）, and to preliminarily explore its mechanism of action. Methods： Sixty-five V-QB patients, with their diagnosis confirmed by clinical examination, were randomized by digital table method into two groups, with 31 patients in the control group and 34 in the treatment group. Cobamamide （2 tablets） was administered orally to all patients, and Psoralea tincture （a self-formulated preparation） was applied externally thrice a day. In addition, for patients in the treatment group, ZSP was given orally, at 5 pills per dose, 3 times every day. The therapeutic course for both groups was 3 months. Patients were re-examined every half-month, and changes in the skin lesions were observed and recorded. The levels of lymphocyte subsets, serum immune globulin, and complement C3 and C4 in patients were determined before and after the therapeutic course and compared with the corresponding indexes determined in 21 healthy subjects, Results： The total effective rate in the treatment group was 82.4%, which was markedly higher than that in the control group （54.8%）, showing a significant difference （P〈O.05）. After treatment, CD4^＋ percentage, CD4^＋/CD8^＋ratio, and blood levels of C3 and C4 increased, while CD8^＋ percentage decreased in the treatment group （P〈0.05 or P〈0.01）. All these indexes remained unchanged in the control group, and the respective comparisons between groups showed significant differences （P〈0.01）. Conclusion： ZSP has a definite clinical effect on the treatment of V-QB but with no evident adverse reactions, and it can increase the CD4^＋ percentage, CD4^＋/CD8^＋ ratio, and the levels of serum C3 and C4, thus regulating the immunity of the organism, which might be one of its mechanisms of action.     

自体CIK细胞对晚期不同阶段肿瘤患者免疫状态和生活质量的影响

目的 探讨自体细胞因子诱导的杀伤细胞（CIK）治疗晚期恶性肿瘤患者,对其免疫状态和生活质量的影响。方法 选取2013年2月至2016年3月合肥市第一人民医院血液肿瘤科收治的42例晚期肿瘤患者,按照预计生存期分为A组（预计生存期<3个月）20例、B组（预计生存期≥3个月）22例,检测治疗前后A、B组患者外周血T细胞亚群的变化。观察治疗前后A、B组患者免疫功能的变化,生活质量改善情况及治疗相关不良反应。结果 CIK细胞治疗后,A组患者CD₃⁺T细胞水平较治疗前降低（P<0.05）;B组治疗后CD₃⁺、CD₄⁺和CD₄⁺/CD₈⁺水平较治疗前明显升高（P<0.05）,CD₈⁺T淋巴细胞亚群水平较治疗前降低（P<0.05）。A、B两组患者治疗后KPS评分分别较治疗前均有提高,其中A组差异无统计学意义（P>0.05）,而B组差异有统计学意义（P<0.05）。42例患者在输注CIK过程中未出现明显不良反应。结论 CIK细胞回输安全、副作用小,自体CIK细胞治疗可提高晚期恶性肿瘤患者的免疫功能,改善其生活质量,但应尽早进行。     

Effect of Compound Glycyrrhizin Injection on liver function and cellular immunity of children with infectious mononucleosis complicated liver impairment

Objective： To investigate the effects of Compound Glycyrrhizin Injection （CGI） on liver function and cellular immunity of children with infectious mononucleosis complicated liver impairment （IM-LI） and to explore its clinical therapeutic effect. Methods： Forty-two patients with IM-LI were randomly assigned, according to the randomizing number table, to two groups, 20 in the control group and 22 in the treated group. All the patients were treated with conventional treatment, but to those in the treated group, CGI was given additionally once a day, at the dosage of 10 ml for children aged below 2 years, 20 ml for 2-4 years old, 30 ml for 5-7 years old and 40 ml for 8- 12 years old, in 100-200 ml of 5% glucose solution by intravenous dripping. The treatment lasted for 2 weeks. T lymphocyte subsets and serum levels of alanine transaminase （ALT）, aspartate aminotransferase （AST） and total bilirubin （TBil） were detected before and after treatment. Besides, a normal control group consisting of 20 healthy children was also set up. Results： Baseline of the percentage of CD3^＋ , CD8^＋ lymphocyte and serum levels of ALT, AST, TBiL in the children with IM-LI were markedly higher, while the percentage of CD 4^＋ lymphocyte and the CD4^＋/CD8^＋ ratio was markedly lower in IM-LI children as compared with the corresponding indices in the healthy children （ P〈0.01 ）. These indices were improved after treatment in both groups of patients, but the improvement in the treated group was better than that in the control group （P〈0.01）. Conclusion： Cellular immunity dysfunction often occurs in patients with IM-LI, and CGI treatment can not only obviously promote the recovery of liver function, but also regulate the immune function in organism.     

IGF-1在妊娠期糖尿病胎盘组织中的表达及其对新生儿T淋巴细胞亚群的影响

目的 分析胰岛素样生长因子-1(IGF-1)在妊娠期糖尿病(GDM)胎盘组织中的表达及其对新生儿T淋巴细胞亚群的影响。方法选取本院GDM患者(研究组)和同期健康孕妇(对照组)各40例。两组孕妇均于胎儿、胎盘分娩后于胎盘中央带取材,行常规HE染色和IGF-1抗免疫组织化学染色,比较两组孕妇胎盘组织中IGF-1表达强度和分娩的新生儿T淋巴细胞亚群指标的差异,并进行相关性分析。结果研究组IGF-1表达强于对照组。IGF-1主要存在于滋养层上皮细胞胞浆和少数血管内皮细胞中。与对照组孕妇新生儿比较,研究组孕妇新生儿CD⁺₃、CD⁺₄、CD⁺₄/CD⁺₈显著较低,CD⁺₈显著较高。相关性分析结果显示孕妇胎盘组织中IGF-1表达强度与CD⁺₃、CD⁺₄、CD⁺₄/CD⁺₈呈正相关,与CD⁺₈呈负相关。结论GDM胎盘组织中的IGF-1表达水平较健康孕妇高,GDM胎盘组织中的高IGF-1表达可显著影响新生儿T淋巴细胞亚群,导致新生儿免疫功能低下。     

Effect of Tiotropium Bromide on Expression of CD_8~＋CD_（25）~＋FoxP_3~＋ Regulatory T Cells in Patients with Stable Chronic Obstructive Pulmonary Disease

The expression of CD8+CD25+FoxP3+ regulatory T cells(CD8+Tregs) in the peripheral blood of patients with stable chronic obstructive pulmonary disease(COPD),and the effect of muscarinic cholinergic receptor antagonist tiotropium bromide on the expression of CD8+Tregs were investigated.Twenty-three patients with moderate to severe stable COPD were enrolled in this study.All patients inhaled tiotropium bromide(18 μg daily) for 3 months.Before and after inhalation of tiotropium bromide,peripheral blood samples were collected from the patients,and T cells were labeled by three-color labeled monoclonal antibodies.Flow cytometry was used to detect the quantity and percentage of CD8+T cells,CD8+CD25+T cells,CD8+Tregs,CD4+T cells,CD4+CD25+T cells and CD4+CD25+FoxP3+ regulatory T cells(CD4+Tregs) respectively.The percentage of CD4+T cells was increased from(27.82±2.18)% to(35.53±1.3)%(t=3.20,P=0.004) in the peripheral blood of patients with stable COPD after inhalation of tiotropium bromide for 3 months,that of CD4+CD25+T cells was decreased from(10.03 ±1.42)% to(4.21 ±0.65)%(t=3.78,P=0.001),and that of CD8+Tregs was increased from(8.41 ±1.68)% to(21.34 ±4.20)%(t=2.72,P=0.013).At baseline,CD8+T cells,CD8+CD25+T cells and CD4+Tregs were detectable in the peripheral blood,but no significant changes were observed after treatment.Linear correlation analysis revealed that the difference before and after treatment in CD4+T cells and CD4+CD25+T cells was negatively correlated with the ratio of change in CD8+Tregs before and after treatment(r=-0.61,P=0.013;r=-0.72,P=0.001 respectively).In the peripheral blood of patients with stable COPD,there was the expression of CD8+Tregs and CD4+Tregs.Muscarinic receptor antagonist,tiotropium bromide,can promote the amplification of CD4+T cells,inhibit the expression of CD25+T cells,and enhance the expression of CD8+Tregs.CD8+Tregs and CD4+Tregs can be used as new indicators to understand the immune status of patients.They are helpful in judging the treatment efficacy and disease immunophenotype.     

孟鲁司特治疗重度慢性阻塞性肺疾病急性加重对免疫功能影响的分析

目的探讨孟鲁司特联合舒利迭治疗重度慢性阻塞性肺疾病（COPD）急性加重对免疫功能的影响。方法将113例重度COPD急性加重期患者,随机分为孟鲁司特联合舒利迭治疗组57例,单纯舒利迭治疗组56例,治疗14 d后,评价所有患者治疗前、后T细胞亚群CD₃⁺、CD₄⁺及CD₄⁺/CD₈⁺的变化。结果治疗组CD₃⁺、CD₄⁺、CD₄⁺/CD₈⁺指标差异有统计学意义（P<0.05）,而对照组无明显变化,药物冶疗安全性好。结论孟鲁司特钠联合舒利迭治疗中重度COPD急性加重期,能有效改善患者免疫功能,提高患者免疫力。     

Clinical Evaluation of Integrative Chinese and Western Medicine in Treating SARS

Objective:To evaluate the effective treatment with integrative Chinese and western medi-cine(ICWM)of severe acute respiratory syndrome(SARS).Methods:The 65 cases of SARS patientswere treated with ICWM and 37 cases with western medicine(WM)alone as the control in our hospitalfrom March 11 to April 30,2003.The results were analyzed,with T subset profile tested for all the 102patients.Results:Sixty-five cases of SARS patients treated with ICWM included 52 mild cases of whom 51cases were cured(98.1％)and no patient died.As to the 13 severe cases in this group,9 were cured(69.2％)and 2 died(15.4％).Of the 37 cases treated with WM alone,16 out of the 18 common cases werecured(88.9％)and 10 out of the 19 severe cases were cured(52.6％),with the other 9 died(47.4％).Comparison between the two groups(P=0.061)showed the difference was close to the value for statisti-cal significance.But considering that the number of samples was small,more cases are needed fot furtherstudy.The immune function examination:in cases     

不同临床类型乙型病毒性肝炎患者淋巴细胞亚群的差异性分析

目的研究分析不同临床类型乙型病毒性肝炎(HBV)患者淋巴细胞亚群的差异性及其临床意义。方法采用流式细胞术检测乙肝病毒携带者、慢性乙型肝炎、急性乙型肝炎、乙型肝炎肝硬化(LC)、原发性肝癌(HCC)患者外周血T淋巴细胞亚群(CD3+、CD4+、CD4+/CD8+)、NK细胞(CD16+CD56+)所占淋巴细胞百分比,并与健康对照组比较。结果与健康对照组比较,乙型肝炎病毒携带者、急性乙型肝炎患者CD3+、CD4+T细胞百分比及CD4+/CD8+比值无统计学差异(P>0.05),慢性乙肝肝炎、LC和HCC患者CD3+、CD4+T细胞百分比及CD4+/CD8+比值逐步递减(P<0.05),以HCC患者下降最为显著。与健康对照组相比,急性乙型肝炎、慢性乙型肝炎患者CD8+T细胞计数显著升高(P<0.05),LC、HCC患者CD8+T细胞则显著下降(P<0.05)。乙型肝炎病毒携带者、急性乙型肝炎患者NK细胞计数与对照组相比,差异无统计学意义(P>0.05),慢性乙型肝炎患者NK细胞计数显著增加(P<0.05),LC、HCC患者NK细胞计数则下降显著(P<0.05)。结论 HBV感染者临床类型不同,各淋巴细胞亚群免疫调节差异有统计学意义,对指导临床治疗及评判预后有一定的临床价值。     

乳腺癌患者细胞免疫水平与预后危险因素的关系及临床意义

目的：探讨乳腺癌患者细胞免疫状态与预后危险因素之间的关系。方法：回顾性分析1997年至2002年收治的110例女性乳腺癌患者的临床资料,分析其一般情况及CD⁺₃T、CD⁺₄T、CD⁺₈T、CD⁺₄/CD⁺₈、淋巴细胞转化率（LTR）的变化及其与乳腺癌肿块大小、淋巴结转移情况及癌胚抗原（CEA）的关系。结果：①乳腺癌不同肿块大小患者中CD⁺₄T、CD⁺₈T、CD⁺₄/CD⁺₈水平差异有统计学意义（P＜0.01或P＜0.05）,在肿块较小时即有CD4+T水平的降低和CD⁺₈T水平的升高,而CD⁺₄/CD⁺₈水平随着肿瘤负荷的增大而降低;②淋巴结转移组［LN(+)］与未转移组［LN(-)］比较,CD⁺₄/CD⁺₈在LN(+)组中显著降低(P＜0.05);CD⁺₃T、CD⁺₄T、CD⁺₈T在LN(+)组中表达增高,CD⁺₃T、CD⁺₈T的升高有统计学意义(P＜0.05或P＜0.01)。 ③CEA在LN(+)组中明显升高(P＜0.05), 在不同肿瘤负荷组间比较差异无统计学意义（P＞0.05）。CEA（+）组和CEA（-）组之间比较,CD⁺₄T升高及CD⁺₄/CD⁺₈水平降低差异均有统计学意义（P＜0.01）, 且CEA与CD⁺₄T及CD⁺₄/CD⁺₈水平密切相关（P＜0.01）。结论：乳腺癌患者存在不同程度的细胞免疫状态紊乱,且与乳腺癌CEA水平及肿块大小、淋巴结转移情况密切相关。     

Clinical Observation on Shuanghuanglian powder in treating viral myocarditis of childrenin treating viral myocarditis of children

Objective: To study immune function of children viral myocarditis and to evaluate the clinical effect of Shuanghuanglian Powder (SHLP) by injection.Methods: The 62 patients of viral myocarditis were divided into two groups randomly, the SHLP group (n = 32) treated with conventional therapy plus SHLP and the conventional treatment group (n = 30) with conventional therapy alone. Their serum antibody of Coxsackie virus group B (COXB-IgM), T-lymphocyte subsets including CD₃ ⁺, CD₄ ⁺, CD₈ ⁺ and CD₄ ⁺/CD₈ ⁺ were determined with ELISA and indirect immunofluorescent assay.Results: COXB-IgM was positive in 39 of the 62 patients, which was significantly different with those of normal controls (P< 0.001). Patients’ serum level of CD₄ ⁺ cells and CD₄ ⁺/CD₈ ⁺ ratio decreased while CD₈ ⁺ increased. After treatment, the recovery of symptoms, signs and immune function in patients of the SHLP group were significantly better than those in patients treated with conventional treatment alone (P<0.01).Conclusion: Immunoregulatory disturbance is involved in children with viral myocarditis and SHLP is an effective drug in the treatment of children viral myocarditis.     

金花清感颗粒联合布地奈德、沙丁胺醇、异丙托溴铵三联雾化吸入疗法在儿童肺炎支原体肺炎中的应用观察

目的 分析金花清感颗粒联合布地奈德、沙丁胺醇、异丙托溴铵三联雾化吸入治疗儿童肺炎支原体肺炎（MPP）的效果。方法 前瞻性选取2022年2月—2023年2月常熟市第二人民医院收治的114例MPP患儿为研究对象,按照随机数字表法分为对照组、研究组,每组57例。对照组给予布地奈德、沙丁胺醇、异丙托溴铵三联雾化吸入治疗,研究组在对照组基础上给予金花清感颗粒,连续治疗10 d评估效果。比较两组症状改善情况、肺功能、临床疗效、气道重塑指标、炎症因子、T淋巴细胞亚群及药物不良反应发生情况。结果 研究组发热消退时间、咳嗽消失时间、肺部啰音消失时间均短于对照组（P <0.05）。研究组治疗前后的达峰时间比、达峰容积比、呼吸频率的差值均高于对照组（P <0.05）。研究组总有效率高于对照组（P <0.05）。研究组治疗前后的气道壁厚度/外径比值、气道面积/总横截面积比值的差值均高于对照组（P <0.05）。研究组治疗前后的超敏C反应蛋白、白细胞介素-6、肿瘤坏死因子-α的差值均高于对照组（P <0.05）。研究组治疗前后的CD4+、CD8+、CD4+/CD8+的差值均高于对照...     

Effect of Excessive Iodine on Immune Function of Lymphocytes and Intervention with Selenium

In order to study the effect of excessive iodine on immune function of lymphocytes and the role of selenium supplementation with excessive iodine intake, the changes of T lymphocyte number, ratio of subsets, activity of natural killer （NK） cells and lymphocytes proliferation response were investigated. 150 female BALB/C mice were randomly divided into 5 groups in terms of their body weight （n=30 in each group）, and 10 of each group were taken as one batch for test. Mice in the 5 groups were orally administrated with iodine 0 （group Ⅰ ）, 1500 （group Ⅱ）, 3000 （group Ⅲ）, 6000 μg/L （group ）, iodine 6000 μg/L plus selenium 0.3 mg/L （group Ⅴ） respectively for 30 days. Lymphocyte proliferation response, CD4^＋/CD8^＋, Th1/Th2 and the activity of NK cells were measured. CD4^＋/CD8^＋ was significantly lower, while lymphocyte proliferation response stronger, and Th1/Th2 and the activity of NK cells significantly higher in group Ⅳ than in group Ⅰ （P〈0.01）. There was no significant difference in all indexes between group Ⅴ and group Ⅰ （P〉0.05）. It was suggested that excessive iodine as exogenous chemical materials can induce disorders of T lymphocyte immune function in mice. 0.3 mg/L selenium supplementation can protect mice against toxicity induced by 6000 μg/L iodine.     

Effects of tripterygii totorum in treating insulin dependent diabetes mellitus patients with islet transplantation

The authors determined both the numbers of T lymphocyte subpopulations in peripheral blood and the concentration of C-peptide in serum before experimentation at 15 days, 1 month, 6 months, and 1 year after islet transplantation in tripterygii totorum (T II) group in which 30 insulin dependent diabetes mellitus (IDDM) patients were given T II and the control group in which 24 IDDM patients did not use any immunosuppressive agents, and 20 healthy individuals were used as the healthy group. Results: Before transplantation, the numbers of T lymphocyte subpopulations such as CD₂, CD₄ in the two groups were lower than those in the healthy group (P<0.01); the ratio of CD₄/CD₈ was between 1.2 and 2.0. After transplantation the numbers of CD₂, CD₄ in the two groups were elevated, particulary in T II group the numbers of CD₂, CD₄ were close to those in the healthy group (P>0.05). The ratio of CD₄/CD₈ in the control group was more than 2.0. The average dose of insulin reduced by 84.2% in 19 patients of the T II group, 2 cases stopped insulin; the peak value of C-peptide was 3.24±1.2 ng/ml. In the control group the average dose of insulin and the peak value of C- peptide was similar to that in the T II group in the first 6 months after transplantation (P>0.05), then the dose of insulin was gradually increased and the concentration of C-peptide began decreasing. One year after transplantation both the dose of insulin and the concentration of C-peptide were close to those before transplantation. The study suggests that T II have immunosuppressive effects in islet transplantation and prolong the survival time of grafts in IDDM patients.     

Changes in T cell subsets and T suppressor cell function and their relationship in human schistosomiasis japonica

Summary The function of spontaneous T suppressor cell (STs) of peripheral blood was examined in 56 patients with schistosomiasis japonica at various stages. The subsets of T cell were simultaneously phenotyped in 46 cases. The percentages of CD₃ ⁺(pan T cell), CD₄ ⁺ (helper/inducer T cell) and CD₈ ⁺ (suppressor/cytotoxic T cell) in patients with acute schistosomiasis japonica were significantly higher than those in the normal controls. In patients with chronic and advanced schistosomiasis japonica, the percentage of CD₈ ⁺ T cell and the function of STs were greatly increased, but the percentage of CD₃ ⁺ T cell and the ratio of CD₄ ⁺/CD₈ ⁺ were obviously reduced. All of these markers changed more significantly in patients with advanced schistosomiasis japonica. The percentage of CD₈ ⁺ T cell in patients with acute schistosomiasis japonica was negatively correlated with the function of STs. In patients with chronic and advanced schistosomiasis japonica the percentage of CD₈ ⁺ T cell was correlated positively and the ratio of CD₄ ⁺/CD₈ ⁺ negatively with the function of STs. The results indicated that the cellular immunity was significantly increased in cases of acute schistosomiasis japonica and decreased in those with chronic or advanced schistosomiasis japonica. The increased CD₈ ⁺ T cell may be principally cytotoxic T cells in patients with acute schistosomiasis japonica, but suppressor T cells in patients with chronic and advanced schistosomiasis japonica. The subsets of T cells and the function of T suppressor cells may play an important role in the immunoregulation of schistosomiasis japonica.     

健脾养胃方联合化疗对胃癌患者T细胞亚群、凝血标志物的影响

目的 探讨健脾养胃方联合化疗对胃癌患者T细胞亚群、凝血标志物的影响。方法 选取2019年7月—2021年12月在河北省中医院就诊的168例胃癌患者。按照随机数字表法分为对照组和研究组，每组84例。对照组采用化疗，研究组采用健脾养胃方+化疗，两组均治疗4个周期。观察两组的临床疗效、毒副反应，比较两组治疗前、后的T细胞亚群（CD4⁺、CD8⁺、CD4⁺/CD8⁺）、凝血标志物［凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、纤维蛋白原（FIB）］及营养状况（血红蛋白、前白蛋白、总蛋白）的变化。结果 研究组治疗总有效率高于对照组（P <0.05）；研究组胃肠道反应、肾毒性发生率均低于对照组（P <0.05）；研究组CD4⁺、CD8⁺治疗前后差值均小于对照组，CD4⁺/CD8⁺治疗前后差值大于对照组（P <0.05）；研究组PT、APTT、FIB治疗前后差值均大于对照组（P <0.05）；研究组血红蛋白、前白蛋白、总蛋白治疗前后差值均小于对照组（P <0.05）。结论 健脾养胃方联合化疗在胃癌患者中疗效显著，有助于维持患者机体免疫力及营养状况，改善凝血功能，减少毒副反应。     

Tc1/Tc2 Imbalance in the Peripheral Blood of Patients with Recurrent Genital Herpes

In order to investigate the IFN-γ and IL-4 expression of CD8^＋T lymphoeytes in the peripheral blood from patients with recurrent genital herpes （RGH） at different clinical periods and their relationship with the pathogenesis of RGH, flow cytometry was used to detect the intracellular cytokines （IFN-γ and IL-4） of CD8^＋ T lymphocytes in the peripheral blood of 30 patients with RGH at acute period, 20 patients with RGH at recovery period and 15 healthy volunteers. The results showed that RGH patients at acute period had a lower percentage of Tcl subsets in peripheral blood than that of healthy controls （P〈0. 001）, especially a remarkable decreased percentage of Tc1 subsets （P〈0. 001） among those RGH patients with recurrent number more than 3 in the recent half a year. Tc1/Tc2 ratio in the RGH patients at acute period was significantly decreased as conapared with normal control group （P〈0.05）. The recurrent number of acute patients in the recent half a year was significantly correlated with the percentage of Tc1 subsets and the ratio of Tc1/Tc2 （P〈 0.05）. A decreased percentage of Tc1 subsets was found among the RGH patients with recurrent number more than 3 in the recent half a year at recovery period in comparison with healthy volunteers （P〈0.05）, and it was significantly correlated with the recurrent number in the recent half a year （P〈0.05）. It is concluded that there are Tc1/Tc2 imbalance and a low level of Tc1 subsets in RGH patients who are relapsing repeatedly in the near period. The low level of Tc1 subsets may be an important factor for the recurrence of RGH and the reactivation of latent herpesvirus infection.     

Effect of TCM combined with chemotherapy on immune function and quality of life of patients with non-small cell lung cancer in stage III-IV

Objective: To observe and compare the effect of traditional Chinese medicine (TCM) combined with chemotherapy (CT) on immune function and quality of life (QOL)of patients with non-small cell lung cancer (NSCLC) in stage III-IV.Methods: One hundred cases with stage III-IV NSCLC were randomly divided into two groups. The treated group (n = 50) received CT combined with TCM, and the control group received CT alone. The percentage of T lymphocyte subset in peripheral blood and the change of natural killer (NK) cell count were observed after treatment. The QOL and tolerance of CT were also compared between the two groups after treatment. Results: In the treated group, CD₃ cell count, CD₄ cell count, CD₄/ CD₈ ratio and NK cell activity were higher than those in control group, while CD₈ cell count in the treated group was lower than that in the control group (P<0.05), and QOL and tolerance of CT in the treated group were also better (P<0.05).Conclusion: TCM combined with CT could raise the patients’ ability in tolerating CT in stage III-IV NSCLC.     

Endothelial nitric oxide synthase traffic inducer in the umbilical vessels of the patients with pre-eclampsia

The expression of endothelial nitric oxide synthase traffic inducer （NOSTRIN） was examined in the umbilical vessels of the patients with pre-eclampsia （PE） to explore its possible role in the pathogenesis of PE. The NOSTR1N rnRNA in umbilical tissues was determined by RT-PCR. The eNOS activity in umbilical vessels was spectrophotometrically detected. NO2 /NO3, the stable metabolic end products of NO, was measured by using nitrate reductase. RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group （P〈0.01）. The activity of eNOS was significantly decreased in PE group [（12.83±3.61） U/mg] than in normal group [（21.72±3.83） U/mg] （P〈0.01）. The level of NO2-/NO3- in PE patients （27.53±7.48） pmol/mg was significantly lower than that of normal group （54.27±9.53） μmol/mg （P〈0.01）. The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE （r=-0.58, P〈0.01）. It was concluded that the level of NOSTR1N expression was increased in umbilical vessel of women with PE, indicating that it may be involved in the pathogenesis of PE.     

经皮125I植入联合化疗在中晚期非小细胞肺癌治疗中的应用及对CD3+、CD4+、CD8+、CD4+/CD8+的影响

目的 探讨经皮放射性碘125粒子（¹²⁵I）植入联合化疗在中晚期非小细胞肺癌（NSCLC）治疗中的应用及对CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺的影响。方法 选取2017年1月-2019年1月咸阳市中心医院收治的120例中晚期NSCLC患者,根据治疗方案分为研究组78例（经皮¹²⁵I植入联合化疗）和对照组42例（同步放化疗）。统计两组基线资料,并记录粒子植入情况及并发症情况,治疗6个月后评估两组临床疗效。比较两组外周血T淋巴细胞亚群CD3⁺、CD4⁺、CD8⁺占比及CD4⁺/CD8⁺治疗前后的变化。统计治疗期间不良反应发生情况。结果 两组性别、年龄、病灶部位、肿瘤直径、病理类型、临床分期及治疗前卡氏评分比较,差异无统计学意义（P >0.05）。研究组91.03%（71/78）患者植入粒子的分布符合术前模拟剂量分布;术后气胸30.77%（24/78）,肺内渗血28.21%（22/78）,发热19.23%（15/78）,粒子移位2.56%（2/78）,围术期均无死亡病例。两组临床疗效比较,差异有统计学意义（P <0.05）,研究组有效率高于对照组。两组治疗后外周血CD3⁺、CD4⁺、CD4⁺/CD8⁺及治疗前后的差值比较,差异有统计学意义（P <0.05）,研究组高于对照组;两组治疗后外周血CD8⁺及治疗前后的差值比较,差异无统计学意义（P >0.05）。两组不良反应（胃肠道反应、粒细胞减少及放射性食管炎发生率）比较,差异无统计学意义（P >0.05）;两组放射性肺炎发生率比较,差异有统计学意义（P <0.05）,研究组低于对照组。结论 经皮¹²⁵I植入联合化疗可有效治疗中晚期NSCLC,可明显改善患者免疫功能,减少不良反应。