癫痫患者头发锌、铜含量变化的研究

测定了55例癫痫患者头发锌、铜含量。发铜含量(6.5±2.1μg/g)显著低于正常对照组(10.6±5.6μg/g),P<0.001;女性患者发锌含量(170.1±56.9μg/g)低于正常对照组(224.2±58.4μg/g),P<0.05;癫痫患者发锌、铜比值(26.6±10.3)较对照组(20.9±11.4)曾高,P<0.05。取样前一年内有癫痫发作,病程为0～5年,癫痫大发作型,脑电图重度或中度异常者,发锌含量降低,与对照组相比有明显差异(P<0.05);抗癫痫药物对发锌影响较对发铜为大。发锌、铜含量及锌、铜比值增高,似表明抗癫痫治疗效果较好。以上结果提示癫痫患者的发锌、铜代谢有异常,其变化与血浆锌、铜含量改变不同。     

泉州地区466名儿童血清锌、铜、铁含量分析

泉州地区466名健康儿童血清锌含量13.56±6.60μmol/L,血清铜17.8±8.12μmol/L,铜/锌比值为1.31,339名儿童血清铁含量19.48±9.27μmol/L。饮用不同水源儿童血清锌、铜、铁含量无显著差异。     

新生儿及其生母血清、头发中的锌、铜含量

本文报导42例足月新生儿及其生母血清、头发中的锌、铜含量。新生儿血清锌81.66μg/dl、铜40.45μg/dl，铜/锌比值为0.49。母亲血清锌43.78μg/dl，非常显著地低于新生儿组；而血清铜268.48μg/dl，铜/锌比值8.71，非常显著地高于新生儿组。新生儿发锌234.27μg/g，非常显著地高于母亲组（171.06μg/g）。新生儿与母亲的血清铜、铜/锌比值、头发铜、铜/锌比值呈显著正相关。新生儿血清铜与发铜呈显著正相关。提示用头发锌、铜含量来判断机体的锌、铜营养状况是可取的。     

冠心病患者血清铜锌含量测定的临床意义

应用火焰原子吸收分光光度法对98例冠心病患者及41例健康人血清中铜、锌的含量进行了测定,其中冠心病高胆固醇患者51例;冠心病高甘油三酯患者44例,结果冠心病高胆固醇组血清铜含量为13.10±1.93μmol/L;血清锌含量为19.66±4.07μmol/L;铜锌比值为0.68±0.14。冠心病高甘油三酯组血清铜含量为12.83±2.67μmol/L;血清锌含量为18.22±5.29μmol/L;铜锌比值为0.73±0.15。与对照组相比,冠心病两组血清铜、铜锌比值显著降低(P<0.01);血清锌含量明显升高(P<0.01),提示血清铜、锌含量,尤其是铜锌比值测定是诊断冠心病的一项客观指标,同时也是冠心病易患因素之一。     

河南省大学生血清和头发中铜锌含量的测定

本文报道了采用原子吸收分光光度法对261名河南省大学新生血清和头发中铜、锌含量的测定结果。其中,血清铜含量和铜/锌比值女性高于男性(P<0.05),血清锌及头发中铜和锌含量均无显著性别差异。根据测定结果作者还提出了本地区青年组人群血清和头发中铜、锌水平与铜/锌比值的正常参考值。     

肺炎患儿血清和尿中锌、铜含量变化

目的　观察肺炎患儿血、尿中微量元素锌 (Zn)、铜 (Cu)含量的变化。方法　应用OLYMPUS 10 0 0型全自动生化分析仪 ,采用比色法检测 4 8例肺炎患儿及 5 1例健康儿童血清、尿中锌、铜含量。结果　健康儿童组血清中锌含量为 ( 18.37± 2 .76 ) μmol/L ,铜含量为 ( 15 .38± 4 .0 3) μmol/L ,铜 /锌比值为 0 .95± 0 .2 8;尿中锌含量为 ( 3.91± 0 .86 ) μmol/L ,铜含量为 ( 0 .8± 0 .35 ) μmol/L。肺炎患儿组血清中锌含量为 ( 12 .4 5± 3.32 ) μmol/L ,铜含量为 ( 17.97± 7.16 ) μmol/L ,铜 /锌比值为 1.4 5± 0 .4 2 ;尿中锌含量为 ( 3.0 5± 1.14 ) μmol/L ,铜含量为 ( 0 .6 9± 0 .31) μmol/L。肺炎组血清、尿中锌含量较健康组明显降低 ,差异有显著意义 (P <0 .0 1) ,血清中铜含量及铜 /锌比值均有明显升高 ,差异有显著意义 (P分别 <0 .0 5、<0 .0 1) ,尿中铜无显著差异 (P >0 .0 5 )。结论　肺炎患儿血清、尿中锌含量降低 ,血清铜浓度增高 ,对指导临床治疗具有参考价值     

冠心病患者血清锌、铜含量的测定及其临床意义

应用火焰原子吸收分光光度法对53例冠心病患者及50例健康人血清中锌、铜的含量进行了测定。结果冠心病组血清铜含量X=15.99±2.64μmol/L;血清锌含量X=19.62±3.78μmol/L。铜/锌比值X=0.83±0.22。与健康人对照组相比,血清铜、铜/锌比值明显降低,血清锌含量显著升高(P均<0.001)。提示血清锌、铜含量测定是诊断冠心病的一项指标。     

肝癌患者血清铜和锌浓度的变化

用原子吸收分光光度计测定了66例健康成年人及17例肝癌惠者血清铜和锌浓度。健康人血清铜平均浓度126.40±23.44μg/100ml((?)±SD),锌116.12±25.60μg/100ml.17例肝癌患者血清铜明显增高,平均浓度为155.83±37.08μg/100ml(P<0.001),血清锌降低为86.72±31.89μg/100ml(P<0.001)。血清Cu/Zn 比值明显的增加(正常值为1.12±0.32;肝癌时为2.15±0.31,P<0.001)。结果提示血清铜锌浓度及比值测定对诊断肝癌有一定意义。     

原子吸收分光光度法测定健康犬血清和肝脏锌、铜含量

用原子吸收分光光度法测得42只健康成龄犬的血清锌含量为14.8±2.0mmol/L,血清铜含量为13.6±.7mmol/L;29只犬的肝锌含量为2.4±0.4mmol/g(干重),肝铜含量为2.8±1.0mml/L(干重)。血清及肝脏锌、铜含量均无明显性别差异。血清锌与肝锌含量间呈正相关,血清铜与肝锌铜含量无明显相关。在一般喂养条件下犬血清锌、铜波动不大。增加锌摄入量可使血清锌和肝锌水平迅速上升,血清铜水平迅速下降,而肝铜变化迟缓。     

人神经胶质瘤组织中的铜锌镁的含量及铜锌比值

本文测定了16例人胶质瘤组织中的铜锌镁含量及铜锌比值,其中恶性肿瘤的铜含量为20.49±4.87μg/g干重,锌为176.97±44.18μg/g干重,镁为650.91±207.88μg/g干重,铜锌比值为0.123±0.04。与健康成人脑组织比较,铜含量及铜锌比值明显降低(P<0.01),镁值升高56.59％,但无统计学意义,锌在二者之间无明显差异。与健康成年组比较,良性胶质瘤三种元素含量都无统计学意义(P>0.05),而早产儿的铜含量有显著性差异(P<0.01)。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.