人涎腺腺样囊性癌嗜神经侵袭的大体形态学研究

目的：探讨人涎晾昧样囊性癌嗜神经侵袭在大体形态学上的发生发展过程。方法：将人涎腺腺样囊性癌肺高转移癌Acc—M细胞系接种于5只裸鼠双侧臀部肌肉内,从荷瘤长出开始,解剖出荷瘤与神经以观察它们的关系,以后每隔5天在同一部位解剖一次,连续观察1个月,同时观察和对比各裸鼠神经受肿瘤侵袭后相应肢体的功能变化。结果：腺样囊性癌发生嗜神经侵袭的方式是,瘤组织细胞以神经干为中心以边生长边包裹边侵袭的方式进行的。结论：腺样囊性癌嗜神经侵袭是以一种特殊的方式进行的,这对临床诊断与治疗具有良好的指导意义。     

COX-2在腺样囊性癌中的表达及意义

目的探讨COX-2在正常涎腺(Normal Salivary,NS)及涎腺腺样囊性癌(Salivary Adenoid Cystic Carcinoma,SACC)中的表达状况及其在SACC发生、发展过程中的作用及意义。方法选择32例SACC组织、11例NS组织,应用SP法免疫组织化学染色检测COX-2的表达情况,分析其在各组间表达的差异。结果 COX-2在11例NS中阳性表达2例,阳性率18.18%。在32例SACC中阳性表达25例,阳性率为78.13%。2组间差异有统计学意义(P<0.05)。在SACC患者按年龄、性别、临床分期、原发部位及病理类型等分组间比较COX-2表达差异无统计学意义(P>0.05)。在按有无侵袭比较COX-2的表达差异有统计学意义(P<0.05)。结论在SACC中,COX-2呈高表达,并与有无侵袭临床参数相关,而与患者的年龄、性别、肿瘤部位、病理类型及临床分期无明显相关性。COX-2可以作为其周围侵袭的一个指标。     

粘着斑激酶在SACC-LM及SACC-83细胞中的活性及表达变化

目的:探讨粘着斑激酶(FAK)在涎腺腺样囊性癌细胞中的活性及表达变化。方法:同时采用逆转录-聚合酶链反应(RT-PCR)和Western blot法检测肺高转移性涎腺腺样囊性癌(SACC-LM)及涎腺腺样囊性癌(SACC)83细胞系中FAK mRNA和蛋白表达;同时测定FAK酶活力。所得实验数据采用SPSS12.0统计软件进行,t检验进行组间比较。结果:SACC-LM细胞中的FAK活性为(18.320±2.050)pmol·min~(-1)·μg~(-1),SACC-83细胞中的FAK活性为(0.873±0.091)pmol·min~(-1)·μg~(-1)。与SACC-83细胞相比,SACC-LM细胞中FAK具有较高的活性(t=13.865,P<0.01)。FAKmRNA在SACC-LM细胞中的表达量为0.883±0.052,明显高于其在SACC-83细胞中的表达量(0.637±0.081,t=4.952,P<0.05)。在SACC-LM细胞中FAK蛋白的表达高于在SACC-83细胞中的表达。结论:FAK蛋白活性变化及蛋白表达与SACC的侵袭有关。     

气管、支气管腺样囊性癌

1974年至 1996年 ,我们共收治 31例气管、支气管腺样囊性癌病人 ,现将其诊治结果报告如下。临床资料　本组 31例中男 18例 ,女 13例 ,男女之比为1 4∶1;年龄 2 3～ 6 7岁 ,平均 42 3岁。病史 1～ 6 0个月。主要症状为咳嗽 2 3例、气短 2 1例及咯血 15例 ,还有哮喘、胸痛、声嘶、发热等。其中 2例因极度呼吸困难 ,1例因大咯血而急诊手术。肿瘤位于气管者 19例、隆凸者 1例、左主支气管 4例、右主支气管 2例、叶支气管及以下 5例。 2 2例行纤维支气管镜(ＦＯＢ)检查 ,活检报告为腺样囊性癌 16例 ,腺癌、类癌、可疑小细胞未分化癌各 1例 ,阴性…     

SD大鼠坐骨神经对腺样囊性癌细胞系ACC-M体外增殖能力的影响

目的：研究SD大鼠坐骨神经对腺样囊性癌ACC-M细胞体外增殖能力的影响。方法：将人腺样囊性癌细胞系（ACC-M）分别与条件培养液和坐骨神经培养液共培养24、48、72h。应用MTT比色法研究SD大鼠坐骨神经对腺样囊性癌ACC-M细胞体外增殖能力的影响。结果：坐骨神经培养液对ACC-M作用48和72h后,ACC-M细胞的增殖明显高于其它组别,而共培养液则对ACC-M细胞的增殖没有明显影响。结论：SD大鼠坐骨神经培养液作用于腺样囊性癌细胞,能有效促进腺样囊性癌细胞的增殖,这可能是腺样囊性癌嗜神经侵袭的基础。     

苦参碱对涎腺腺样囊性癌生长抑制的实验研究

目的:研究分化诱导剂苦参碱(Matrine)在体外对人涎腺腺样囊性癌细胞生长抑制的影响。方法:用不同浓度的Mat分别处理涎腺腺样囊性癌ACC-M细胞后,镜下观察癌细胞的生长情况;测定软琼脂克隆形成率;MTT(噻唑蓝)法测定生长抑制率。结果:①不同浓度苦Mat处理后细胞的生长明显受到抑制;②细胞生长抑制率随Mat的浓度增加而增加。结论:Mat对涎腺腺样囊性癌细胞的生长有明显的抑制作用。     

Bcl-2、NF-KB在腮腺腺样囊性癌中的表达及临床意义

目的:探讨Bcl-2和NF-KB在腮腺腺样囊性癌中的表达及意义。方法:应用免疫组化SP法检侧49例腮腺腺样囊性癌和20例正常腮腺组织中Bcl-2和NF-KB的表达情况,统计学分析采用χ2检验,P<0.05判断为具有显著性差异。结果:Bcl-2和NF-KB的表达强度显著高于正常腮腺组织(P<0.05),Bcl-2、NF-KB的表达与病理无关(P>0.05),与TNM分期有关,Bcl-2和NF-KB两者存在正相关性(P<0.05,Kappa=0.387)。结论:在腮腺腺样囊性癌的发生、发展过程中NF-KB通过上调Bcl-2的表达发挥作用。     

乳腺腺样囊性癌1例

乳腺腺样囊性癌(adenoid cystic carcinoma,ACC)是种罕见的原发性乳腺癌,乳腺ACC病例不足0.1%[1]。文献报道大多数乳腺ACC呈ER、PR、HER-2阴性[2]。然而,与三阴性乳腺浸润性导管癌和涎腺的ACC具有高侵袭的生物学行为不同,乳腺ACC是一种预后较好的乳腺浸润性癌,淋巴结转移和远处转移的发生率很低,十年生存率可达90%以上[3-4]。     

外耳道腺样囊性癌2例临床分析

<正>腺样囊性癌(adenoid cystic carcinoma; ACC)可原发于外耳道软骨段,来源于耵聍腺导管上皮或肌上皮。临床上外耳道腺样囊性癌十分罕见,现对本院收治的2例外耳道腺样囊性癌患者进行分析如下。1病例特点患者1,女,41岁。因右耳疼痛8个月,伴右侧枕部放射痛,无耳鸣、耳闷胀感及面瘫,以"外耳道肿物"为主诉于2016年6月28日入住我科。查体:整个右侧外耳道被1. 0 cm×1. 0 cm大小的肿物阻塞,未窥及鼓膜。     

气管腺样囊性癌七例

气管腺样囊性癌临床上少见。我院从 1997～ 2 0 0 1年 12月共手术治疗 7例 ,现报告如下。1　临床资料与方法本组共 7例 ,男 3例 ,女 4例 ;年龄 30～ 5 2岁。所有患者均表现为咳嗽、进行性喘息 ,呼吸困难。发病至就诊时间为 6个月～ 4年。出现呼吸三凹征 4例。病变位于颈段气管 3例 ,胸段气管 4例。病变长度 2 .5～ 8.0 cm ,累及甲状腺或食管各1例。行手术治疗 7例 ,其中颈段气管切除、气管永久性造瘘 2例 ;行气管切开、肿瘤刮除术 2例 ,术后给予放疗 ;二次行颈段气管姑息环切术 1例 ,再次复发给予放射治疗 ;行隆突成形 +右肺中下叶切除术 1…     

Response to paclitaxel in adenoid cystic carcinoma of the salivary glands

BACKGROUND: Paclitaxel is not considered to be an active drug in adenoid cystic carcinoma (ACC) of the salivary glands. We report 2 consecutive cases of patients with ACC who responded to paclitaxel. METHODS: The patients were: (1) a 58-year-old man with recurrent ACC who developed pulmonary metastases, had progressive disease after a good response to first-line chemotherapy, and then achieved a partial response to weekly single- agent paclitaxel; and (2) a 46-year-old woman with extensive thoracic ACC metastases who achieved a significant response after 2 cycles of paclitaxel chemotherapy. RESULTS: The first patient died of progressive disease approximately 4 months after completing paclitaxel therapy, and the second patient had disease control after 6 cycles of paclitaxel. CONCLUSIONS: Systemic weekly paclitaxel produced a significant response in 2 patients with ACC of the head and neck, and its use in this disease merits further study.     

Lung metastasis resection of adenoid cystic carcinoma of salivary glands

Background: Adenoid cystic carcinoma is a rare tumour originating from the exocrine mucous glands, known for its high propensity for distant metastases. The value of lung metastasis resection from adenoid cystic carcinoma of salivary glands origin is evaluated. Methods: A retrospective study was conducted on patients undergoing surgery for primary adenoid cystic carcinoma of the salivary glands between 1982 and 2006. Patients were excluded who had primary tumour macroscopic incomplete resection or were lost at follow-up. From a database of 50 eligible patients, 27 were identified as having presented a tumour recurrence during follow-up; in 20 it was first diagnosed in the form of distant metastases, and in 7 in the form of loco-regional recurrence. Nine patients who presented isolated lung recurrence underwent complete lung metastasectomy. Demographic data, pathologic characteristics and operative and postoperative record were reviewed, as well as updated survival. Results: Twenty-six men and 24 women with a median age of 57 years (range 33–79) underwent radical surgery for adenoid cystic carcinoma during the study period. In 20 patients, at a median free interval time of 3 years (range 1–12), a distant metastasis relapse was observed. Nine patients with a median free interval time of 5 years (range 1–12) underwent lung metastasectomy: five had single metastasis resection, one multiple mono-pulmonary and three multiple and bilateral. In six of these patients a new disease recurrence was noted: four patients underwent further lung metastasectomy, but in all of them progression of the disease was observed. Mean survival of the population as a whole resulted as being 16 years (SE = 1.4) with an actuarial survival of 77% at 5 years, 66% at 10 years and 56% at 15 years. Mean survival of patients having presented with distant metastases resulted as being 11 years (SE = 2.2). Mean survival after appearance of distant metastases resulted as being 72 months (SE = 15.8) in the 9 patients treated by metastasectomy, and 62 months (SE = 15.1) in the 11 who did not have metastasis resection. Conclusions: Patients with adenoid cystic carcinoma could be frequently encountered with disease recurrence confined to the lung. The impact of complete lung metastasis resection on the course of the disease, however, is yet to be determined.     

NCAM和DPC4的表达与胆管癌神经浸润的关系

为探讨NCAM和DPC4在胆管癌神经浸润中的作用,笔者采用免疫组化技术(SP法)对52例胆管癌标本及16例正常胆管组织中NCAM和DPC4的表达进行检测，结果显示，NCAM高表达及DPC4低表达的胆管癌神经浸润率高，NCAM低表达及DPC4高表达的胆管癌神经浸润率低。提示NCAM高表达及DPC4低表达,在胆管癌的神经浸润转移的分子机制中可能有重要的作用;NCAM可能成为临床预测胆管癌神经倾向的生物学指标,并对判断胆管癌恶性程度与预后有较重要的临床意义。