丙型肝炎患者外周血T淋巴细胞亚群的变化

丙型肝炎患者外周血Ｔ淋巴细胞亚群的变化李灼亮，郑茉莉，谢庆，涂荫国，黄振国空军广州医院传染病科广州５１０６０２一般认为［１～４］慢性乙型肝炎患者外周血Ｔ淋巴细胞亚群的变化，可反映患者全身免疫状态，ＣＤ８＋细胞显著升高，ＣＤ４＋／ＣＤ８＋比值下降，可能...     

狼疮性肾炎患者淋巴细胞亚群比率和免疫球蛋白水平的变化

目的 探讨狼疮性肾炎（ＬＮ）患者的淋巴细胞亚群和免疫球蛋白（Ｉｇ）的变化及其意义。方法采用淋巴细胞的膜抗原双标记染色法及ＥＬＩＳＡ，对６０例ＬＮ患者的细胞与体液免疫功能进行前瞻性研究。结果①与对照组相比较，活动期ＬＮ患者的ＣＤ３＋ＣＤ４＋细胞的比率（Ａ组：伴肾病综合征者）为（ｌ６．３ ±７．９）％，Ｂ组（不伴肾病综合征者）为（２０．８±１０．１）％］和ＣＤ１６＋＋ＣＤ５６＋细胞的比率［Ａ组为（８．６±５．７）％，Ｂ组为（１５．２±６．２）％明显减少；ＣＤ３+ ＣＤ8+”细胞的比率［Ａ组为（４８．３±１０．７）％，Ｂ组为（３５．９±９．８）％］明显增高；ＣＤ３+ ＣＤ４+ ／ＣＤ３+ ＣＤ８＋细胞的比值＜１（Ａ组为０．４３±０．２１，Ｂ组为０．８７±０．２５），且Ａ组与８组相比较差异明显（Ｐ＜０．０５）。②与活动期ＬＮ患者相比较，治疗后处于稳定期的 ＬＮ患者 ＣＤ３＋ＣＤ４＋细胞的比率卜组为（２８．８±８．ｌ）％，Ｂ组为（３２．８± ７．ｌ）％］和 ＣＤ１６＋＋ＣＤ５６＋细胞的比率［Ａ组为（１８．９ ± １２．５）％，Ｂ组为（２４．０±８． ９）％，以及 ＣＤ３＋ＣＤ４＋／ＣＤ３＋ＣＤ８＋细胞的比值（Ａ组为 ０．９７±２．３，Ｂ组     

肾小球疾病患者外周血T淋巴细胞亚群的观察

细胞免疫在肾小球肾炎的发生发展过程中发挥着重要的作用[1] ,但慢性肾小球肾炎患者外周血Ｔ淋巴细胞亚群在临床上尚无一致的结论[2 ] ,可能与运用不同的检测方法有一定关系。本文运用流式细胞仪 ,检测肾病患者外周血Ｔ淋巴细胞亚群 ,藉以探讨对二者之间的关系。1　临床资料共检 15 4例 ,年龄 14～ 78岁 ,中位数 38岁。慢性肾炎41例 ,肾病综合征 44例 ,慢性肾衰 (原发性肾小球 ) 5 4例 ,狼疮性肾炎 (ＬＮ ) 7例 ,紫癜性肾炎 8例。另外 ,根据血浆白蛋白情况将肾病综合征分为白蛋白 >2 5ｇ/Ｌ组 (Ａ组 )和白蛋白 <2 5ｇ/Ｌ组 (Ｂ组 ) ,Ａ组血…     

应用武—系列单克隆抗体研究癫痫患者淋巴细胞亚群的变化

本文借助单克隆抗体系列检测了30例癫痫患者外周血中T淋巴细胞亚群,B细胞和单核巨噬细胞。结果表明,患者T_3和T_4细胞百分率均低于正常人,T_8细胞百分率增高,T_4/T_8比值明显降低。B细胞和单核巨噬细胞数未见明显改变,提示本病存在细胞免疫功能异常。同时证明用两种单克隆抗体系列检测结果无显著性差异。     

溃疡性结肠炎患者外周血淋巴细胞亚群检测的临床价值

目的 探究外周血淋巴细胞亚群检测在溃疡性结肠炎（ulcerative colitis,UC）诊断中的临床价值.方法 随机选择解放军总医院第一附属医院2011年1月至2013年12月溃疡性结肠炎确诊患者70例,健康体检人群39人,检测其外周血CD3、CD4、CD8、CD4/CD8四项免疫学指标.以SPSS16.0进行独立样本t检验,分析各项指标的诊断价值.结果 溃疡性结肠炎患者与健康人群相比,CD3、CD8差别不明显,CD4、CD4/CD8具有明显差别（P＜0.05）.结论 淋巴细胞亚群的检测对溃疡性结肠炎实验室诊断具有重要临床价值.     

桥本甲状腺肿原位淋巴细胞亚群的观察

桥本甲状腺肿又称慢性淋巴细胞甲状腺炎，它是一种自身免疫性疾病〔１，２〕。除甲状腺局部细胞免疫反应引起大量淋巴细胞浸润、滤泡形成外，同时伴有甲状腺滤泡上皮萎缩变性和增生亢进，晚期伴有纤维增生，有的病例可伴发甲亢或甲状腺癌，或恶性淋巴瘤〔１～３〕。探讨本...     

乙型流感患者外周血白细胞和淋巴细胞亚群变化分析

目的 探讨乙型流感患者外周血白细胞和淋巴细胞亚群的变化特点,为乙型流感的诊断、治疗和预后判断提供实验室依据.方法 采用全血细胞分析和流式细胞分析法分别检测47例乙型流感患者急性期和恢复期的外周血白细胞及淋巴细胞亚群,并与38名健康人比较.结果 乙型流感轻症患者外周血白细胞总数在急性期显著下降,恢复期迅速上升,而重症患者外周血白细胞总数、中性粒细胞百分比及绝对值在急性期显著上升,恢复期迅速下降;所有乙型流感患者淋巴细胞总数、CD3、CD4、CD8、CD19在急性期显著下降,恢复期迅速上升;轻症患者的NK细胞绝对值在急性期与恢复期都与健康对照组无显著差异,而重症患者NK细胞绝对值在急性期显著下降,恢复期迅速上升.结论 乙型流感患者在急性期出现白细胞总数及中性粒细胞的显著上升,而NK细胞绝对值的大幅降低可以提示病情重症化倾向.     

13例SARS患者淋巴细胞亚群和功能的观察

严重急性呼吸综合征 (severeacuterespiratorysyndrome,SARS)的病原体为新型冠状病毒 ,发病机制有待进一步研究〔1〕 。为探讨其发病机理并为临床治疗提供依椐 ,在收治SARS期间 ,我们对其中部分患者外周血淋巴细胞亚群和功能进行了检测。材料和方法研究对象 :按卫生部疾病控制中心所制订的传染性非典型肺炎临床诊断标准 (试行 ) 〔2〕,临床诊断为严重急性呼吸综合征普通型发病期患者 1 3例 ,男性 9例 ,女性 4例 ,平均年龄 2 8.49±4 .69岁 ,均以发热、畏寒和 或寒战、咳嗽无痰或少量白色粘痰 ,外周血白细胞正常或降低 ,胸片示肺部渗出性…     

带状疱疹患者外周血T淋巴细胞亚群分析

带状疱疹患者外周血Ｔ淋巴细胞亚群分析李大宁，余红上海第二医科大学附属新华医院皮肤科上海２０００９２带状疤疹是一种由水痘一带状疱疹病毒（ＶＺＶ）感染引起的疾病，机体对ＶＺＶ的特异性免疫目前认为主要是细胞免疫。本文采用流式细胞仪（ＦＣＭ）技术测定带状疤疹...     

高血压病患者左室肥厚及其相关因素分析

目的:观测高血压病(EH)患者的主动脉根部内径(AOD)、脉压(PP)及其他相关因素,结合文献分析其与左室肥厚间的关系,以指导临床治疗和预测血管意外发生的危险性。方法:对58例EH患者,应用超声心动图测量并计算其左室后壁厚度(LVPWT)、室间隔厚度(TVST)、AOD,用血压仪测量并计算其血压各参数值;根据测算结果将本组58例分为左室肥厚组和无左室肥厚组,并将相关数据进行组间统计学比较。结果:两组间比较,年龄、病程、脉压(PP)、收缩压(SBP)及AOD值有显著性差异(P<0.01),而舒张压(DBP)和平均动脉压(MAP)值无明显差异(P>0.05)。结论:PP增大及AOD增宽与左室肥厚呈正相关;PP在高血压左室肥厚中起重要作用,早期发现有利于心脑血管疾病的防治。     

原发性高血压患者巨细胞病毒感染及与血管并发症关系 …

目的 探讨原发性高血压患者巨细胞病毒感染及与血管并发症之间的关系。方法 应用间接酶联免疫吸附试验（ＥＬＩＳＡ）检测了１０５例原发性高血压患者血清人巨细胞病毒（ＨＣＭＶ）特异性抗体。结论 研究结果提示原发性高血压患者存在较高的活动性巨细胞病毒感染，ＨＣＭＶ活动性感染似与血管并发症有一定关联。     

Ventilatory responses in patients with essential hypertension.

We investigated the ventilatory responses to hypoxia and hypercapnia in patients with essential hypertension (HT) as compared with healthy subjects (NV). Further, to evaluate the contribution of the peripheral chemoreceptors to ventilatory response, we used a withdrawal test. Hypoxic ventilatory drive (HVR) was measured as the parameter A denoting the shape of VI (inspiratory minute ventilation)-PETO2 (end-tidal PO2) curve which was calculated by the empirical equation: VI = V0 + A/(PETO2-32). Hypercapnic ventilatory drive (HCVR) was measured as the parameter S denoting the shape of the VI-PETCO2 (end-tidal PCO2) relation which was calculated by the empirical equation: VI = S(PETCO2-B). There were no significant differences in the parameters of HVR and HCVR between NV and HT. A positive correlation between A/BSA and S/BSA was found to be significant in NV (r = 0.873, p less than 0.05). Conversely, there was no significant correlation between A/BSA and S/BSA (r = 0.547) in HT. On the other hand, the withdrawal responses (delta VI/BSA and % delta VI:delta VI/VI x 100%) were obtained from the magnitude of depression in ventilation caused by two breaths of O2 in hypoxic hypercapnia. In the withdrawal responses, delta VI/BSA and % delta VI in HT were significantly higher than those in NV. A/BSA significantly correlated with delta VI/BSA (NV, r = 0.684, p less than 0.05; HT, r = 0.648, p less than 0.05) in both NV and HT. However, delta VI/BSA in HT tended to be higher than that in NV, under the same value of A/BSA. These results suggested that the peripheral chemoreceptor activity was augmented in HT.     

Heart rate variability and its determinants in patients with severe or mild essential hypertension.

How cardiac autonomic nervous control is related to the severity of essential hypertension in patients receiving long-term antihypertensive therapy is not well known. The aim of this study was to examine heart rate variability (HRV), a non-invasive measure of cardiac autonomic function, in patients with long-term and medically treated mild and severe essential hypertension and healthy control subjects, and to assess the clinical determinants of HRV in these patients. Thirty-four patients with severe essential hypertension (SEHT) and 29 with mild essential hypertension (MEHT) as well as healthy age- and sex-matched control subjects were studied. HRV was assessed from 10 min ECG-recordings during paced (0.2 Hz) breathing at rest and expressed as time and frequency domain measures. In the SEHT group time (SDNN, RMSSD) and frequency domain measures (total power, low-frequency (LF) power and high-frequency (HF) power of HRV in absolute units, and LF and HF power of HRV in normalized units) of HRV were significantly lower when compared with those of the control group. The MEHT and control groups did not differ from each other with respect to time or frequency domain measures of HRV. Comparison between the hypertensive groups showed that SDNN, total power, LF power and HF power were lower in the SEHT group compared with the MEHT group (P<0.05 for all). Among hypertensive patients RR-interval, age, gender, systolic finger blood pressure and diastolic office blood pressure as well as 24-h blood pressure were significant determinants of HRV. In conclusion, we found that the severity of chronic essential hypertension seems to be related to the severity of impairment of cardiac autonomic control.     

高血压病患者心脏指数、外周阻力及外周血液淋巴细胞β受体的检测

本实验应用放射配体结合法和超声心动图法分别测定了稳定型高血压病患者的外周血液淋巴细胞β受体密度和亲合力以及心脏指数和外周总阻力,并与正常人组进行了比较。结果显示高血压患者外周血液淋巴细胞β受体密度比正常人组值低(238±107对318±130 fmol/10~7细胞,P<0.05);二组之受体与配体复合物的平衡解离常数KD分别为2.62±1.25,3.31±1.14nm,二组间无显著差异(P>3.05)。高血压患者的心脏指数尚无显著性降低(3.92±1.06对4.54±1.09 L/min/M~2,P>0.05)而外周总阻力则明显增高(2811±711对1798±484 dynes sec.cm~(-3).M~2,P<0.01)。鉴于外周血液淋巴细胞β受体密度与心肌β受体密度呈正相关且受内源性儿茶酚胺的调节,本结果提示在稳定性高血压阶段也必然存在着心肌β受体密度的下降。我们认为这可能是高血压时尽管心脏所接受的肾上腺激素能刺激增加,但心排出量却不相应地增加甚至有下降趋势的原因之一。     

T and B lymphocyte markers in effusions of patients with non-Hodgkin's lymphoma

T and B cells were sought in effusion fluids of 13 patients with lymphoma. In T cell lymphomas (four cases) morphologically abnormal cells that formed E rosettes were present. In B cell lymphomas (nine cases) morphologically abnormal cells were present in only two cases, however immunological studies showed a reduction in T cells and monoclonal light chain immunoglobulin expression in six of nine cases.     

Abnormal endothelium-dependent vascular relaxation in patients with essential hypertension

BACKGROUND. Endothelium regulates vascular tone by influencing the contractile activity of vascular smooth muscle. This regulatory effect of the endothelium on blood vessels has been shown to be impaired in atherosclerotic arteries in humans and animals and in animal models of hypertension. METHODS. To determine whether patients with essential hypertension have an endothelium-dependent abnormality in vascular relaxation, we studied the response of the forearm vasculature to acetylcholine (an endothelium-dependent vasodilator) and sodium nitroprusside (a direct dilator of smooth muscle) in 18 hypertensive patients (mean age [+/- SD], 50.7 +/- 10 years; 10 men and 8 women) two weeks after the withdrawal of antihypertensive medications and in 18 normal controls (mean age, 49.9 +/- 9; 9 men and 9 women). The drugs were infused at increasing concentrations into the brachial artery, and the response in forearm blood flow was measured by strain-gauge plethysmography. RESULTS. The basal forearm blood flow was similar in the patients and controls (mean +/- SD, 3.4 +/- 1.3 and 3.7 +/- 0.8 ml per minute per 100 ml of forearm tissue, respectively; P not significant). The responses of blood flow and vascular resistance to acetylcholine were significantly reduced in the hypertensive patients (P less than 0.0001); maximal forearm flow was 9.1 +/- 5 ml per minute per 100 ml in the patients and 20.0 +/- 8 ml per minute per 100 ml in the controls (P less than 0.0002). However, there were no significant differences between groups in the responses of blood flow and vascular resistance to sodium nitroprusside. Because the vasodilator effect of acetylcholine might also be due to presynaptic inhibition of the release of norepinephrine by adrenergic nerve terminals, the effect of acetylcholine was assessed during phentolamine-induced alpha-adrenergic blockade. Under these conditions, it was also evident that the responses to acetylcholine were significantly blunted in the hypertensive patients (P less than 0.03). CONCLUSIONS. Endothelium-mediated vasodilation is impaired in patients with essential hypertension. This defect may play an important part in the functional abnormalities of resistance vessels that are observed in hypertensive patients.