Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae

The study of tissue-derived Mycobacterium leprae provides insights to the immunopathology of leprosy and helps identify broad molecular features necessary for mycobacterial parasitism. A major membrane protein (MMP-II) of in vivo-derived M. leprae previously recognized (Hunter, S.W., B. Rivoire, V. Mehra, B.R. Bloom, and P.J. Brennan. 1990. J. Biol. Chem. 265:14065) was purified from extracts of the organism and partial amino acid sequence obtained. This information allowed recognition, within one of the cosmids that encompass the entire M. leprae genome, of a complete gene, bfr, encoding a protein of subunit size 18.2 kD. The amino acid sequence deduced from the major membrane protein II (MMP-II) gene revealed considerable homology to several bacterioferritins. Analysis of the native protein demonstrated the iron content, absorption spectrum, and large native molecular mass (380 kD) of several known bacterioferritins. The ferroxidase-center residues typical of ferritins were conserved in the M. leprae product. Oligonucleotides derived from the amino acid sequence of M. leprae bacterioferritin enabled amplification of much of the MMP-II gene and the detection of homologous sequences in Mycobacterium paratuberculosis, Mycobacterium avium, Mycobacterium tuberculosis, Mycobacterium intracellulare, and Mycobacterium scrofulaceum. The role of this iron-rich protein in the virulence of M. leprae is discussed.     

A rapid ELISA for the diagnosis of MB leprosy based on complementary detection of antibodies against a novel protein-glycolipid conjugate

Despite the widespread use of multidrug therapy for treatment, delays in clinical recognition and under-reporting of leprosy indicate that Mycobacterium leprae transmission is continuing. Thus, leprosy is likely to persist as a significant burden on health systems in many regions. In this study, we combined 2 previously characterized leprosy antigens, leprosy IDRI diagnostic-1 (LID-1) and ND-O, into the single fusion complex (ND-O–LID) and determined the serum antibody responses of leprosy patients from Colombia and the Philippines. Following confirmation that antibodies recognized each component within the conjugate, we assessed the performance of a rapid enzyme-linked immunosorbent assay (ELISA) system (Leprosy Detect^TM fast ELISA; InBios International, Inc., Seattle, WA, USA) based on ND-O–LID capable of generating results within 1.5 hours of sample addition. We found ELISA results correlated with the bacteriological index and Ridley–Jopling categorization, with lepromatous leprosy patients having the highest responses, while those of borderline tuberculoid patients were lower. Multibacillary (MB) leprosy patients were distinguished with a high degree of sensitivity (95.7%) and specificity (93.2%), suggesting that this ELISA could potentially replace invasive and insensitive skin slit smear procedures that require expert microscopic examinations. Due to the speed and robustness of this assay, we believe this is an excellent tool for detecting MB leprosy patients in a simple and highly-quantitative manner.     

Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay using membrane antigens of Leishmania (Viannia) braziliensis

This study evaluated the reactivity of membrane antigens of Leishmania (Viannia) braziliensis for the diagnosis of ACL by enzyme immunoassay (EIA). Promastigotes of L. (V.) braziliensis were grown in medium 199 and lysed in a sonicator. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting showed that specific proteins of L. (V.) braziliensis (apparent molecular weights 36 kDa and 48–56 kDa) were recognized by sera from ACL patients. These proteins were eluted from the SDS-PAGE and tested in EIA-IgG with sera from ACL patients, healthy individuals, patients with toxoplasmosis, paracoccidioidomycosis, syphilis, tuberculosis, leprosy, and Chagas disease. The EIA-IgG with membrane antigens allowed us to distinguish patients with ACL from healthy individuals and patients with other diseases (P < 0.0001), and showed a sensitivity of 93.3% and specificity of 90.8%, not including Chagas disease patients. 2D-SDS-PAGE followed by Western blotting was performed to improve the characterization of the antigens, and showed a component with isoelectric points near the acid pH side and apparent molecular weights of 48–56 kDa. The results showed good sensitivity and specificity of EIA-IgG with membrane antigens, indicating their potential use for diagnosis of ACL, as well as seroepidemiological surveys and follow-up of clinically cured patients.     

Detection of Mycobacterium leprae antigens in urine of leprosy patients by monoclonal-antibody-based sandwich immunoradiometric assay and avidin-biotin based immunoblotting

Urine samples from 54 leprosy patients and 12 controls were tested for the presence of Mycobacterium leprae antigens using monoclonal-antibody-based sandwich immunoradiometric antigen-capture assay. Excettion of antigen was observed in the urine of patients with tuberculoid borderline and lepromatous types of leprosy. M. leprae-specific 12 kDa protein antigen was seen in the urine of 56% of leprosy patients. M. leprea quasi-specific 35 kDa protein antigen (immunodominant) in that of 33% of the patients and mycobacterial cross-reactive polysaccharide antigen of 30–40 kDa in the urine of 54% of the patients. At least one of these antigens was found in the urine of about 90% of the patients. None of the controls was positive for any of the antigens. It appears that weakly immunogenic antigens are excreted to a far greater extent than the strongly immunogenic one. The avidin-biotin based immunoblotting also revealed antigens in the urine of leprosy patients. The fact that about 90% of the patients showed some antigen positivity, as determined by monoclonal-antibody-based sandwich antigen-capture assay, may be of value in the diagnosis of leprosy, particularly the hitherto poorly diagnosed tuberculoid leprosy.     

应用ND-O-BSA-ELISA对贵州省贵阳市的麻风病患者家属及周围人群抗体水平的检测研究

目的 了解贵州省贵阳市麻风患者家属及周围人群血清中麻风抗体水平情况,为麻风患者家属及周围人群的重点随访提供有力依据。方法 将检测对象的一般信息和应用ND-O-BSA-ELISA检测的IgM、MMP-IgG和LID-IgG抗体检测值导入Excel电子数据库,再用SPSS 18.0分析软件对抗体检测值进行χ2检验和t检验。结果 麻风患者家属中3人的IgM为阳性,周围人群无阳性病例,但两组不同人群的IgM抗体变化差异无统计学意义（P>0.05）;在麻风病患者家属组中,有50人MMPIgG为阳性,占23.47%,有44人LID-IgG为阳性,占20.66%;周围人群中有12人MMP-IgG阳性,占4.90%,11人LID-IgG阳性,占4.90%。两组不同人群的MMP-IgG和LID-IgG抗体变化差异具有统计学意义（P<0.001）。结论 应用ND-O-BSAELISA方法能有效检测麻风病患者家属及周围人群的麻风特异性抗体水平。在体检和随访时不仅要关心麻风病患者本人,也要关注其家属,同时兼顾周围人群。     

浙江省温州市基本消灭麻风病后新发现的麻风病例流行病学分析

目的探讨1992-2008年浙江省温州市麻风病基本消灭后的麻风病流行规律和特征。方法资料来源于全市麻风病例登记表、麻风病年报表和麻风病例个案病历。结果1992-2008年17年间共发现新麻风病例19例。年平均发现率0.022/10万。2008年发现7例，年发现率0.09/10万。呈较大幅度上升。男女性别比为18∶1，平均发现年龄（35.779.66）岁，平均延迟期（73.5675.66）个月，多菌型（MB）和少菌型（PB）之比为3.75∶1。病例中流动人口占68.42%。结论1992-2008年温州市麻风病基本消灭后疫情一直比较平稳，2008年呈较大幅度上升，温州市麻风病疫情仍处于低流行状态。     

The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection

Carbohydrates of pathogen antigens have been disrupted by periodate oxidation, in order to reduce nonspecific bindings and improve serodiagnosis of parasite infections. In the present study, the enzyme-linked immunosorbent assay (ELISA) was carried out with filariform larvae antigen treated, or not treated, with sodium metaperiodate. Groups of sera from patients with Strongyloides stercoralis infection, with other intestinal parasites and a normal control, were used. The oxidation of Strongyloides stercoralis glycosylated epitopes reduced the seroreactivity of sera from patients with S. stercoralis infection as demonstrated by ELISA, with a decrease in sera optical densities. The number of cross-reactions of IgG and IgE-ELISAs increased by 12% and 16%, respectively, after antigen treatment with metaperiodate. This was more often observed in patients infected with Schistosoma mansoni and hookworm. Moreover, the IgG depletion from sera tested by IgE-ELISA led to the detection of previous false-negative samples from S. stercoralis–infected patients.     

T细胞斑点试验AB抗原检测结果对结核感染患者诊断意义探讨

目的探讨结核感染T细胞斑点试验(T-SPOT.TB)中AB两种抗原检测结果在临床结核感染患者诊断中的意义。方法选取2013年6~11月住院治疗并最终确诊活动性结核的96例患者为研究对象,采集其外周血进行T-SPOT.TB检测,将检测结果与结核菌素试验(PPD)及最终诊断结果进行对比,并对AB两种抗原检出结果的一致性及其阳性斑点分布特点进行分析。结果 T-SPOT.TB灵敏度为97.9%,特异度为87.8%,准确度为94.5%,阳性预测值(PPV)为94.0%,阴性预测值(NPV)为95.6%,均显著高于PPD检测结果(P0.01)。T-SPOT.TB两种抗原A和B对检测结果阳性率无统计差异,阳性斑点分布特征分析显示抗原A阳性斑点数与结核活动性呈正相关趋势,有较高的特异度;而抗原B则分别在高、低阳性斑点区呈现活动性结核检出的高峰,具有更好的敏感度。结论 T-SPOT.TB试验对活动性结核诊断有高度的敏感度和特异度,且AB两种抗原表现出不同的阳性检出规律和意义。T-SPOT.TB试验可为临床结核病的筛查和大规模流行病学调查提供新的技术分析手段。     

Development of competitive enzyme immunoassay for the detection of influenza A(H5) virus antibodies

An indirect enzyme immunoassay (EIA) using the purified fraction of surface viral glycoproteins (GP) as an antigen for solid phase sensitization was not shown to be a specific method for the differential detection of influenza A(HS) (HS-Ab) virus antibodies (Abs) due to total conservative epitopes in the structure of GPs of influenza A(H5) and A(H1NI) viruses. The cross activity of some monoclonal Abs (MAbs) to influenza A(H5) and A(HIN1) viruses, which had been obtained at the Research Institute of Influenza, was proof of the presence of total immunodominant determinants in the structure of influenza H1 and H5 virus hemagglutinin (HA). In this connection, an EIA, which was based on the competition of influenza A(H5) H5-Ab virus HA-specific MAbs in the test sera for an association with influenza A(H5) virus, was proposed for the subtype-specific detection of H5 Ab. Comparison of the results of competitive EIA (cEIA), microneutralization (MN) test and HA inhibition test (HAIT) (using equine red blood cells) in the examination of sera obtained from 44 volunteers immunized with inactivated vaccine containing influenza A/Indonesia/5/2005 (H5N1) virus showed the high sensitivity and specificity of cEIA in detecting H5-specific Abs. The effectiveness of cEIA for the sera strictly positive for the content of H5 Abs was close to that of MN test and was 9-34% higher than HAIT (depending on those used in the analysis of H5 virus antigens). cEIA may be proposed to assess new influenza vaccines as an additional laboratory test. Since the infectious virus is not used during cEIA, it may be recommended for the serodiagnosis of influenza A(H5) at practical virological laboratories.     

Strain-Dependent Modulation of Ultrasound Speed in Articular Cartilage Under Dynamic Compression

Mechanical properties of articular cartilage may be determined by means of mechano-acoustic indentation, a clinically feasible technique for cartilage diagnostics. Unfortunately, ultrasound speed varies in articular cartilage during mechanical compression. This can cause significant errors to the measured mechanical parameters. In this study, the strain-dependent variation in ultrasound speed was investigated during dynamic compression. In addition, we estimated errors that were induced by the variation in ultrasound speed on the mechano-acoustically measured elastic properties of the tissue. Further, we validated a computational method to correct these errors. Bovine patellar cartilage samples (n = 7) were tested under unconfined compression. Strain-dependence of ultrasound speed was determined under different compressive strains using an identical strain-rate. In addition, the modulation of ultrasound speed was simulated using the transient compositional and structural changes derived from fibril-reinforced poroviscoelastic (FRPVE) model. Experimentally, instantaneous compressive strain modulated the ultrasound speed (p < 0.05) significantly. The decrease of ultrasound speed was found to change nonlinearly as a function of strain. Immediately after the ramp loading ultrasound speed was found to be changed –0.94%, –1.49%, –1.84%, –1.87%, –1.89% and –2.15% at the strains of 2.4%, 4.9%, 7.3%, 9.7%, 12.1% and 14.4%, respectively. The numerical simulation revealed that the compression-related decrease in ultrasound speed induces significant errors in the mechano-acoustically determined strain (39.7%) and dynamic modulus (72.1%) at small strains, e.g., at 2.4%. However, at higher strains, e.g., at 14.4%, the errors were smaller, i.e., 12.6% for strain and 14.5% for modulus. After the proposed computational correction, errors related to ultrasound speed were decreased. By using the correction, with e.g., 2.4% strain, errors in strain and modulus were decreased from 39.7% to 7.2% and from 72.1% to 35.3%, respectively. The FRPVE model, addressing the changes in fibril orientation and void ratio during compression, showed discrepancy of less than 1% between the predicted and measured ultrasound speed during the ramp compression. (E-mail: juha.toyras@kuh.fi)     

Screening Pneumocystis carinii pneumonia in non–HIV-infected immunocompromised patients using polymerase chain reaction

We evaluated the clinical value of polymerase chain reaction (PCR) for screening Pneumocystis carinii pneumonia (PCP) in non–HIV-infected immunocompromised patients. We retrospectively analyzed PCR and Grocott's methenamine silver (GMS) staining on 1044 clinical specimens obtained from 756 patients. Positive rates of PCR and GMS staining in sputum specimens were 21.1% and 9.5%, respectively (P < 0.01), and in bronchoalveolar lavage (BAL), specimens were 31.9% and 25.5%, respectively. Among 5 patient groups, the highest GMS staining and PCR positive rates were observed in immunosuppressed patients. In 28 GMS staining-positive patients, positive rates of PCR and GMS staining after a short anti-PCP treatment were 39.3% and 17.9%, respectively. In 5 patients with positive PCR but negative GMS staining results in initial sputum examination, GMS staining result turned positive in subsequent BAL specimens. In conclusion, PCR is sensitive for detection of Pneumocystis in sputum specimens and is useful for screening PCP in non–HIV-infected high-risk patients.     

Serum anti-p53 antibody detection in carcinomas and the predictive values of serum p53 antibodies, carcino-embryonic antigen and carbohydrate antigen 12-5 in the neoadjuvant chemotherapy treatment for III stage non-small cell lung cancer patients

BackgroundThe serum p53 antibody (s-p53 Ab) is a valuable prognostic factor for carcinomas, but its common detection method, based on enzyme linked immunosorbent assay (ELISA), needs to be improved due to low sensitivity. Although neoadjuvant chemotherapy (NACT) is widely used in the treatment of non-small cell lung cancer (NSCLC) in China, forecasting chemoresistance is still a pressing problem.MethodsHybrid phage and wild-type p53 protein (wt p53 protein) were produced before the establishment of phage-ELISA and p53-ELISA. S-p53 Abs of 829 patients with various types of cancer was detected by a double ELISA system. 47 ΙΙΙ stage NSCLC patients treated with mitomycin, vindesine and cisplatin (MCV)-based NACT were chosen for s-p53 Abs, carcino-embryonic antigen (CEA) and carbohydrate antigen (CA) 12-5 predictive value analysis.ResultsThrough the combination of p53-ELISA and phage-ELISA (p53-phage ELISA), the sensitivity of s-p53 Abs in lung, breast, colorectal, gastric, esophageal, liver and ovarian cancer increased to 39.0%, 33.3%, 41.7%, 32.1%, 30.9%, 23.1% and 43.2% respectively. S-p53 Abs proved to correlate with nodal involvement, TNM stage, histological type (in lung cancer) or tumor size (in gastric cancer). As for the 47 ΙΙΙ stage NSCLC treated with NACT, s-p53 Abs and CA12-5 remarkably decreased after NACT treatment (P = 0.034 and P = 0.007) and pre-NACT low s-p53 Abs correlated with high objective chemoresponse rate (P = 0.016).Conclusionsp53-phage ELISA system has an edge over single p53-ELISA. S-p53 Abs level correlates with cancer patients' clinicalpathological parameters and can predict the chemoresponse of ΙΙΙ stage NSCLC patients during MCV-based NACT treatment.     

Detection of anti-phenolic glycolipid I antibodies by ELISA and gelatin particle agglutination test in leprosy in São Paulo (SP), Brazil

A total of 214 serum samples obtained from leprosy patients (96 with the lepromatous form, 36 with the tuberculoid form, 33 with the indeterminate form, and 19 with the borderline form) before and during chemotherapy, from household contacts (n = 18) and from controls (n = 12) were submitted to the M. leprae particle agglutination test (MLPA) and to enzyme-linked immunosorbent assay (ELISA) for the detection of anti-phenolic glycolipid (PGL I) antibodies. ELISA was more sensitive for all clinical forms of leprosy, with greater seropositivity for the lepromatous form (54.64%). For 88 patients with the lepromatous form, we noted that the shorter the time of treatment (≤ 3 years), the higher the percentage of seropositive results (P ≤ 0.01). The present results suggest that both tests could be used to monitor leprosy treatment and in epidemiologic surveys.     

BCG多克隆抗体对麻风病诊断价值的探讨

目的　探讨卡介苗 (BCG)多克隆抗体对麻风病的早期诊断、鉴别诊断及治疗效果评估的价值。方法　选择临床经组织学和细菌学检查确诊的麻风病患者 5 0例 ,可疑组 2 4例 ,治愈复查组 4 9例 ,对照组 2 1例 ,采用BCG多克隆抗体免疫组化染色技术检测各组中的BCG抗原 ;同时应用抗酸染色对确诊组进行麻风病原菌检查 ,并比较两种方法的优越性。结果　BCG阳性检出率确诊组 80 % (4 0 5 0 ) ,可疑组 5 4 2 % (13 2 4 ) ,治愈复查组 16 3% (8 4 9) ,对照组 9 5 % (2 2 1)。确诊组中的抗酸染色阳性病例数为 36例 ,阳性率为 72 %。另外 ,分析了确诊组中 以上的阳性检出率 ,BCG多克隆抗体检测法为 6 4 % (32 5 0 ) ,抗酸染色检测法为 4 8% (2 4 5 0 ) ,两者比较差异显著 (P <0 0 5 )。结论　BCG多克隆抗体免疫组化染色法优于抗酸染色 ,可用于对麻风病的早期诊断、鉴别诊断及治疗效果的评估 ,是一项操作简单 ,敏感性较高的检测项目     

Acute non‐hemolytic transfusion reactions and HLA class I antibody: advantages of solid phase assay compared with conventional complement‐dependent assay

To evaluate the specific reactivity of HLA Class I antibodies (HLA‐I Abs) in acute non‐hemolytic transfusion reactions (ANHTRs) using solid phase assays (SPAs) and conventional complement‐dependent lymphocyte cytotoxicity test (LCT). ANHTRs are major issues in transfusion medicine. Anti‐leukocyte antibodies have been implicated as one of the causative agents of transfusion‐related acute lung injury (TRALI) and febrile reaction. Antibodies to HLA Class I and/or Class II (HLA Abs) have been intensively studied using SPAs for TRALI, but not for febrile reaction. About 107 patients and 186 donors associated with ANHTRs were screened for HLA Abs by SPAs such as enzyme‐linked immunosorbent assay (ELISA) and the Luminex method. When HLA‐I Ab was detected, its specific reactivity was evaluated by comparing its specificity identified by the Luminex method using recombinant HLA molecules and cognate HLA antigens (Ags), as well as LCT with or without anti‐human globulin (AHG). The incidences of HLA Abs were as high as 32·7% of patients' serum samples and 16% of donors' serum samples. The incidence of HLA‐I Abs did not differ significantly between cases of febrile and allergic reactions. However, HLA‐I Abs associated with febrile reaction showed a significantly higher rate of possessing specific reactivity to cognate HLA Ags than those associated with allergic reactions. In addition, the Luminex method enabled the detection of HLA‐I Abs much earlier than AHG‐LCT in serum samples from a patient with febrile reaction and platelet transfusion refractoriness (PTR). SPAs seem more useful than AHG‐LCT for evaluating reactivity of antibodies in ANHTR cases.     

The prognostic value of CD44 expression in gastric cancer: A meta-Analysis

Several studies have been conducted to examine the association between CD44 expression and the prognosis of gastric cancer (GC). However, the conclusions remain controversial. We conducted a meta-analysis study of 16 published studies with 2403 patients to evaluate the correlation between CD44 expression and clinicopathological characteristics and overall survival of the GC patients. Pooled odds ratios (ORs) and their 95% confidence intervals (CIs) were used to assess the correlation of CD44 expression with the clinicopathological features of GC patients. Hazard ratios (HRs) with their 95% confidence intervals (CIs) were used to assess the association between CD44 and prognosis of GC patients. Total CD44 expression was detected in ten studies, and CD44v5 and CD44v6 expressions were detected in one and five papers, respectively. The results revealed that CD44 expression was associated with some clinicopahological features, such as lymph node metastasis (pooled OR = 1.81, 95% CI = 1.44–2.34, P = 0.000), distant metastasis (pooled OR = 3.29, 95% CI = 1.90–5.67, P = 0.001) and TNM stage (Pooled OR = 1.84, 95% CI = 1.13–2.99, P = 0.014). Moreover, we also found that GC patients with positive CD44 expression had a worse prognosis than the ones with negative CD44 expression (HR = 1.93, 95% CI = 1.54–2.42, P = 0.000). In stratified analysis, the combined HR with CD44 and CD44v6 was 2.20 (95% CI = 1.81–2.67) and 1.70 (95% CI = 1.00–2.90), respectively. These results suggested that positive CD44 expression could predict a lower overall survival rate and could be an independent dangerous prognostic factor in GC patients.     

Evaluation of a rapid enzyme immunoassay system for serologic diagnosis of Mycoplasma pneumoniae infection

A 5-min qualitative membrane enzyme-linked immunoassay (EIA) from Remel (Mycoplasma pneumoniae immunoglobulin G (IgG)/IgM Antibody Test System) was evaluated for its ability to detect IgG and IgG at levels indicating active or recent infection. Specimens from 131 patients were evaluated using an immunofluorescent antibody assay (IFA) to determine IgG and IgM titers and the membrane EIA. An enzymelinked immunosorbent assay (ELISA) performed by a reference laboratory was used for discrepancy resolution. There were 34 IgM positive specimens (titer ⩾ 1:16), 19 IgG positive specimens (titer ⩾ 1:64), and 78 negative specimens. Compared with IFA and/or ELISA, the membrane EIA was 97% sensitive for the detection of IgM and 79% sensitive for the detection of IgG. Of the 78 specimens called negative, 17 specimens had IgG titers (⩽1:32) or an ELISA result indicating prior exposure, and the membrane EIA called seven of 17 (41%) positive. For the detection of both IgG and IgM, the membrane EIA had a sensitivity of 91 %, specificity of 91%, and positive and negative predictive values of 87 and 93%, respectively. The Remel membrane EIA is a rapid and reliable assay for the diagnosis of active or recent M. pneumoniae respiratory tract infections.     

肺炎嗜衣原体三种不同抗原的临床诊断价值评价

目的 研究肺炎嗜衣原体3种重组抗原Cpn0146、Cpn0147及Cpn0308应用于肺炎嗜衣原体感染血清学诊断中的价值.方法 构建pGEX6p-2/Cpn0146、Cpn0147、Cpn0308重组质粒,并诱导表达重组蛋白,采用间接ELISA法和免疫印迹法(Western-blot)分析其免疫原性及其免疫反应性.建立分别以3种重组蛋白为包被抗原的间接ELISA法,与肺炎嗜衣原体IgG商品诊断试剂盒平行检测183份呼吸道感染患者的血清标本及32份沙眼衣原阳性患者血清标本,比较各方法检出准确性及阳性率.结果 GST-Cpn0146、Cpn0147、Cpn0308免疫兔血清中特异性IgG抗体效价滴度分别达1:6 400、1:12 800和1:12 800以上;以3种重组蛋白为包被抗原的ELISA法准确性分别为92.3%、94.5%、96.7%.各方法检测出阳性率结果显示,Cpn0146组的阳性率为38.8%(71/183)、与肺炎嗜衣原体IgG试剂盒的阳性率比较差异有统计学意义(x~2=12.07,P<0.05);Cpn0147阳性率40.9%(75/183),与肺炎嗜衣原体IgG试剂盒的阳性率比较差异有统计学意义(x~2=8.10,P<0.05);Cpn0308阳性率44.8%(75/183),与肺炎嗜衣原体IgG试剂盒的阳性率比较差异无统计学意义(x~2=0.57,P>0.05).结论 Cpn0308在肺炎嗜衣原体感染血清学诊断实验中表现出良好的准确性(96.2%)和较高的阳性率(44.8%),可望用于进一步研制肺炎嗜衣原体诊断试剂盒.