Antibacterial effect of plant extracts against Helicobacter pylori

The aim of this work was to evaluate the antibacterial effect of plant extracts as alternative and[sol ]or as active agents supporting antibiotics for treating Helicobacter pylori infection. The effect of either, ethanolic or aqueous extracts from 17 plant materials were studied against one H. pylori standard strain and 11 clinical isolates using a disc diffusion test and by evaluating the minimum inhibitory concentration (MIC) on solid media. An inhibitory activity against H. pylori strains was recorded in a large percentage of tested plants. MIC values of ethanolic extracts were from two to four concentration steps lower than the aqueous ones. In particular, ethanolic extracts of Cuminum cyminum L. and Propolis expressed MIC90 values of 0.075 mg/mL. The results show a significant in vitro effect of plant extracts against H. pylori that could be considered a valuable support in the treatment of the infection and may contribute to the development of new and safe agents for inclusion in anti-H. pylori regimens.     

Susceptibility of Helicobacter pylori to essential oil of Dittrichia viscosa subsp. revoluta

The essential oil of Dittrichia viscosa subsp. revoluta and its fractions were assessed for anti-Helicobacter activity. The essential oil was isolated by hydrodistillation, submitted to flash column chromatography and analysed by gas chromatography, gas-chromatography coupled to mass spectrometry and (13)C-nuclear magnetic resonance. The anti-Helicobacter activity was determined by incorporation of the crude essential oil and oxygenated fractions of the oil into the culture medium. At a concentration of 0.025 microL/mL no recovery was registered when one of the oxygenated fractions of the oil, mainly constituted by 3-methoxy cuminyl isobutyrate (about 40%), was used. This fraction revealed a higher activity against the six H. pylori strains tested when compared with the other oxygenated fractions. The crude essential oil at a concentration of 0.33 microL/mL reduced the initial population of H. pylori CCUG 15818 of 8.52 +/- 0.30 log(10) cfu/mL to 7.67 +/- 0.22 log(10) cfu/mL. The susceptibility of several Helicobacter pylori strains to the oxygenated fraction of Dittrichia viscosa subsp. revoluta essential oil suggests the possible use of these natural products in combating this widespread infection.     

Comparative analysis between Chamomilla recutita and corticosteroids on wound healing. An in vitro and in vivo study

The comparison of chamomile and corticosteroids for treating ulcers was done in vitro and in vivo. The experimental groups were: control; chamomile recutita; triamcinolone acetonide and clobetasol propionate. For the in vitro study the cell viability of fibroblasts cultured for 24 h in media conditioned by the substances was obtained by the MTT reduction analysis. For the in vivo study, 125 male rats were submitted to experimental ulcers treated or not (control) by the substances tested. At 1, 3, 5, 7 and 14 days later 5 animals of each group were sacrificed. The lesions were analyzed by means of clinical observation and histological wound-healing grading. Data were compared by ANOVA (p 相似文献   

白蔹正丁醇提取物抗菌作用研究

目的：体外检测中药白蔹正丁醇提取物的抗菌作用。方法：采用琼脂平板扩散法定性和体外药基法定量检测提取物抗菌活性,同时分析不同浓度白蔹正丁醇提取物抗菌的量效反应关系。结果：白蔹正丁醇提取物的MIC值为2．5mg／mL,浓度和抗菌有量效反应关系。结论：白蔹正丁醇提取物有一定的抗菌作用。     

Selective Antibacterial Activity of Patchouli Alcohol Against Helicobacter pylori Based on Inhibition of Urease

The aim of this study is to evaluate the antibacterial activity and urease inhibitory effects of patchouli alcohol (PA), the bioactive ingredient isolated from Pogostemonis Herba, which has been widely used for the treatment of gastrointestinal disorders. The activities of PA against selected bacteria and fungi were determined by agar dilution method. It was demonstrated that PA exhibited selective antibacterial activity against Helicobacter pylori, without influencing the major normal gastrointestinal bacteria. Noticeably, the antibacterial activity of PA was superior to that of amoxicillin, with minimal inhibition concentration value of 78 µg/mL. On the other hand, PA inhibited ureases from H. pylori and jack bean in concentration‐dependent fashion with IC₅₀ values of 2.67 ± 0.79 mM and 2.99 ± 0.41 mM, respectively. Lineweaver‐Burk plots indicated that the type of inhibition was non‐competitive against H. pylori urease whereas uncompetitive against jack bean urease. Reactivation of PA‐inactivated urease assay showed DL‐dithiothreitol, the thiol reagent, synergistically inactivated urease with PA instead of enzymatic activity recovery. In conclusion, the selective H. pylori antibacterial activity along with urease inhibitory potential of PA could make it a possible drug candidate for the treatment of H. pylori infection. Copyright © 2014 John Wiley & Sons, Ltd.     

A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.)

Chamomile (Matricaria recutita L., Chamomilla recutita L., Matricaria chamomilla) is one of the most popular single ingredient herbal teas, or tisanes. Chamomile tea, brewed from dried flower heads, has been used traditionally for medicinal purposes. Evidence-based information regarding the bioactivity of this herb is presented. The main constituents of the flowers include several phenolic compounds, primarily the flavonoids apigenin, quercetin, patuletin, luteolin and their glucosides. The principal components of the essential oil extracted from the flowers are the terpenoids alpha-bisabolol and its oxides and azulenes, including chamazulene. Chamomile has moderate antioxidant and antimicrobial activities, and significant antiplatelet activity in vitro. Animal model studies indicate potent antiinflammatory action, some antimutagenic and cholesterol-lowering activities, as well as antispasmotic and anxiolytic effects. However, human studies are limited, and clinical trials examining the purported sedative properties of chamomile tea are absent. Adverse reactions to chamomile, consumed as a tisane or applied topically, have been reported among those with allergies to other plants in the daisy family, i.e. Asteraceae or Compositae.     

黄连对幽门螺杆菌的体外抗菌活性研究

目的研究黄连乙醇提取液的成分及小檗碱含量,比较黄连和盐酸小檗碱对幽门螺杆菌(Helicobacter pylori)体外抗菌活性。方法使用反相高效液相色谱(RP-HPLC)法鉴定黄连乙醇提取液中成分及小檗碱含量,选取盐酸小檗碱做体外对比抑菌实验,观察黄连和盐酸小檗碱的抑菌效果并进行比较。结果与结论黄连对H.pylori的体外抗菌活性与小檗碱的相比,黄连较好。     

几种蜂蜜对幽门螺旋杆菌的体外抗菌作用

目的研究不同蜂蜜对幽门螺旋杆菌的体外抗菌作用。方法取龙眼蜜、荔枝蜜、八叶五加蜜、桂花蜜、野菊花蜜、桉树蜜、红树林蜜等7种蜂蜜,按两倍稀释法进行药敏试验,观察最低抑菌浓度和最低杀菌浓度。结果桉树蜜抑菌作用最强(M IC 1∶8),红树林蜜抑菌作用最弱(M IC 1∶2),其它蜜抑菌作用相当(M IC 1∶4);桉树蜜还具有一定的杀菌作用(MBC 1∶2),其他蜂蜜则无此作用。结论7种蜂蜜在体外均可抑制幽门螺旋杆菌,尤以桉树蜜最佳,为开发和利用蜂蜜辅助治疗疾病提供了一定依据。     

In vitro anti-Helicobacter pylori potential of methanol extract of Allium ascalonicum Linn. (Liliaceae) leaf: susceptibility and effect on urease activity

The crude methanol extract of the leaf of Allium ascalonicum was screened in vitro against fi ve strains of Helicobacter pylori (Hp) (ATCC 24376, UCH 97001, UCH 97009, UCH 98026 and UCH 99039) for antibacterial activity by the agar diffusion method in Mueller-Hinton agar supplemented with de fi brinated horse blood. All the strains were inhibited by the extract to varying degrees. The minimum inhibitory concentrations (MICs) of the extract against all the tested strains ranged from 6.25 to 12.5 mg/mL. The effects of increasing concentrations of the extract on the urease activity of three of the Helicobacter pylori strains were investigated further. The results showed that increasing the concentration of the extract decreased the urease activity of all the strains tested. Phytochemical screening of the plant showed that it contains alkaloids, cardiac glycosides and saponins. The anti-Hp activity observed is discussed in relation to the chemical constituents reportedly isolated from these plants and their traditional uses. The result of this work suggests that Allium ascalonicum has some therapeutic potential against Helicobacter pylori infection, which could be explored for patients with gastroduodenal disorders.     

In vitro anti‐Helicobacter pylori activity of usnic acid

Eradication of Helicobacter pylori is an important objective in overcoming gastric diseases. Many regimens are currently available but none of them could achieve 100% success in eradication. Medicinal lichen is used in the treatment of gastric ulcer in local folk medicine in Anatolia (Turkey). The present study was performed to assess the in vitro effects of usnic acid from Usnea dasypoga against clinical isolates and standard H. pylori strains and their minimum inhibitory concentrations (MICs). A total of 38 strains was assayed for anti‐H. pylori activity. The agar dilution method was used for the determination of usnic acid and clarithromycin resistance. Six (16.2%) clinical isolates were resistant to usnic acid and five (13.5%) were resistant to clarithromycin. Dual susceptibility to usnic acid and clarithromycin rate was detected as very high (97.3%). Usnic acid has a strong and dose‐dependent activity against H. pylori strains. The synergism between usnic acid and clarithromycin may be effective in the treatment of H. pylori infection. Copyright © 2009 John Wiley & Sons, Ltd.     

半夏泻心汤及其拆方抗幽门螺杆菌作用的研究

目的:探讨半夏泻心汤及其拆方抗Hp的作用.方法:运用琼脂扩散法、液体稀释法定性和定量检测半夏泻心汤及其拆方对Hp的抗菌作用 结果:在琼脂扩散法中,半夏泻心汤组对Hp抗菌作用优于辛温组和甘温组,差异有统计学意义(P＜0.05);半夏泻心汤组略优于苦寒组,差异无统计学意义(P＞0.05).在液体稀释法中,半夏泻心汤对Hp的最低抑菌浓度(MIC)与最低杀菌浓度(MBC)均为15.63mg/mL;辛温组和苦寒组对Hp的最低抑菌浓度(MIC)与最低杀菌浓度(MBC)均分别为62.50 mg/mL、31.25mg/mL.均在实验检测的浓度范围内(1.95 mg/mL ～ 250.00 mg/mL).结论:半夏泻心汤有较明显的抗菌作用,其中起优势作用的药物以苦寒药为主.     

Antibacterial activity of Harungana madagascariensis leaf extracts

Aqueous extracts of the leaf of Harungana madagascariensis were analysed phytochemically and evaluated for antimicrobial activity against strains of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa. Glycosides, tannins, saponins, flavonoids and alkaloids were detected in the plant material. B. subtilis, E. coli and S. typhi, but not Ps. aeruginosa, showed susceptibility at MICs of 2.0 and 15.6 mg/mL; and MBCs of 2.0-3.9 mg/mL and 15.6-31.3 mg/mL, respectively, for the cold and hot extracts. Staph. aureus showed susceptibility only to the hot extract. Concentrations of 2.5-10.0 mg/mL of the cold extract killed over 7 log(10) of the test bacterial population within 30-60 min of exposure. The hot extract needed higher concentrations and longer treatment to achieve similar levels of bacterial cell killing. The results provide a rationalization for the traditional use of H. madagascariensis leaf extracts for the treatment of gastrointestinal disorders.     

幽门螺杆菌的中医药治疗概况

幽门螺杆菌（Helicobacter pylori,H.pylori）感染是导致消化系统疾病的主要因素之一。西药治疗所出现的耐药性、毒副作用以及治后所引起的肠道菌群紊乱、易复发等,使众人目光转向祖国医学,迫使中医临床者寻求一种方便、安全、有效的治疗方法。现就近年来中医药治疗幽门螺杆菌做一综述。     

Antibacterial activity of medicinal plant extracts against periodontopathic bacteria

This study was performed to evaluate the antibacterial activity of Althaea officinalis L. roots, Arnica montana L. flowers, Calendula officinalis L. flowers, Hamamelis virginiana L. leaves, Illicium verum Hook. fruits and Melissa officinalis L. leaves, against anaerobic and facultative aerobic periodontal bacteria: Porphyromonas gingivalis, Prevotella spp., Fusobacterium nucleatum, Capnocytophaga gingivalis, Veilonella parvula, Eikenella corrodens, Peptostreptococcus micros and Actinomyces odontolyticus. The methanol extracts of H. virginiana and A. montana and, to a lesser extent, A. officinalis were shown to possess an inhibiting activity (MIC < or = 2048 mg/L) against many of the species tested. In comparison, M. officinalis and C. officinalis extracts had a lower inhibiting activity (MIC > or = 2048 mg/L) against all the tested species with the exception of Prevotella sp. Illicium verum methanol extract was not very active though it had a particular good activity against E. corrodens. The results suggest the use of the alcohol extracts of H. virginiana, A. montana and A. officinalis for topical medications in periodontal prophylactics.     

Antibacterial and antifungal activity of juniper berry oil and its selected components

Juniper berry oil is stated to possess a wide spectrum of pharmacological activities and its monographs are included in some National Pharmacopoeias. The antibacterial and antifungal activity of the oil was reported by some authors. In our study we estimated the antibacterial and antifungal activity of three different juniper berry oils and their main components. All the micro-organisms used in this experiment were isolated from patients of Regional Hospital of Gdańsk and some of them showed resistance against commonly used antibiotics. Only one of the oils (labelled A) revealed good antimicrobial properties. None of the single oil components was a stronger antibacterial and antifungal inhibitor than the oil A itself. Our data suggest that the antimicrobial activity of juniper oil A is the result of either the specific composition of the oil A (highest concentration of (-)-alpha-pinene, p-cymene and beta-pinene) or activity of a single non-identified compound. The presence of an adulterant in the oil was excluded.     

Antimicrobial activity of an Amazon medicinal plant (Chancapiedra) (Phyllanthus niruri L.) against Helicobacter pylori and lactic acid bacteria

The potential of water extracts of the Amazon medicinal plant Chancapiedra (Phyllanthus niruri L.) from Ecuador and Peru for antimicrobial activity against Helicobacter pylori and different strains of lactic acid bacteria such as Lactobacillus acidophilus, Lactobacillus casei and Lactobacillus plantarum was investigated. H. pylori was inhibited by both water extracts in a dose dependent manner, whereas lactic acid bacterial growth was not affected. Both extracts contained ellagic acid and hydroxycinnamic acid derivatives and exhibited high free radical scavenging linked-antioxidant activities (89%). However, gallic acid was detected only in the Ecuadorian extract. Preliminary studies on the mode of action of Chancapiedra against H. pylori revealed that inhibition may not involve proline dehydrogenase-based oxidative phosphorylation inhibition associated with simple mono-phenolics and could involve ellagitannins or other non-phenolic compounds through a yet unknown mechanism. This study provides evidence about the potential of Chancapiedra for H. pylori inhibition without affecting beneficial lactic acid bacteria.     

序贯与三联疗法根除幽门螺杆菌的临床应用比较

 目的 比较由雷贝拉唑联合阿莫西林、克拉霉素以及替硝唑组成的10 d序贯疗法与三联疗法根除幽门螺杆菌的疗效、依从性、安全性及经济学比较。方法 将幽门螺杆菌阳性的90例消化性溃疡患者随机分为以雷贝拉唑为基础的10 d序贯疗法组(n=45)和三联疗法组(n=45),治疗结束4周后复查¹⁴C-UBT,评估幽门螺杆菌根除情况,并对各方案进行成本-效果分析。结果 共85例患者按方案完成治疗。10 d序贯疗法组幽门螺杆菌根除率按意向治疗(ITT)和按方案(PP)分析均高于三联疗法组(ITT：91.1%对73.3％,P<0.05;PP：95.3％对78.6％,P<0.05);10 d序贯疗法组不良反应发生率(PP：14.0％对11.9％,P<0.05)与三联疗法组相似;成本-效果分析显示,序贯治疗组的成本-效果比值（C/E）低于对照组。结论 以雷贝拉唑为基础的10 d序贯疗法具有疗效高、可提高幽门螺杆菌根除率等优势。     

幽门螺杆菌阴性消化性溃疡与出血关系的研究

目的探讨幽门螺杆菌(H.pylori)阴性消化性溃疡与出血之间的关系及其临床特点。方法选择急诊内镜诊断为胃溃疡和/或十二指肠溃疡患者160例,按照是否合并出血分为出血组和不伴出血组,各80例,并分析影响H.pylori阴性消化性溃疡合并出血的因素。结果出血组H.pylori阴性率为40%,不伴出血组H.pylori阴性率为14%,2组比较有显著性差异(P<0.05)。H.pylori阴性消化性溃疡合并出血与复合性溃疡、黏膜炎症和炎症程度存在明显的相关性,与十二指肠溃疡、胃溃疡、溃疡直径、溃疡分期、黏膜萎缩、肠化生和淋巴组织增生无明显相关性。结论 H.pylori阴性消化性溃疡在消化性溃疡出血中所占的比例较高,有复合性溃疡、黏膜炎症和炎症严重的患者可能更易并发消化道出血,需要引起消化专科医生的重视。     

鱼腥草贮藏过程中挥发油抑菌效果变化的研究

目的:研究鱼腥草地上部分和地下部分在贮藏过程中挥发油抑菌效果的变化。方法:采用二倍稀释法测定鱼腥草挥发油抑菌活性和最低抑菌浓度(MIC)。结果:鱼腥草鲜样挥发油的抑菌效果最强,对金黄色葡萄球菌、八叠球菌的MIC均为1/2×10-3ml/ml,在贮藏过程中随着时间的延长MIC逐渐增大。结论:鱼腥草挥发油抑菌活性随着贮藏时间的延长而呈下降趋势。     

Antibacterial activity of plant extracts from azuki beans (Vigna angularis) in vitro

This study was undertaken to examine the antimicrobial property of azuki beans (Vigna angularis). The water extracts of green, black and red colored azuki beans showed antibacterial effects against Staphylococcus aureus, Aeromonas hydrophila and Vibrio parahaemolyticus. In contrast, the extract of white azuki beans showed no inhibition towards any of the microorganisms examined. The extracts of colored azuki beans contained larger amounts of polyphenols including proanthocyanidins than the extracts of white azuki beans. The counts of S. aureus cells, inoculated in the medium containing the extracts of colored azuki beans, were significantly reduced in comparison with those of control and white azuki beans after 24 h (p < 0.05). These results suggest that polyphenols including proanthocyanidins in colored azuki beans may be responsible for their antibacterial activity.