胰岛素样生长因子及其结合蛋白与妊娠期高血压疾病的相关性研究

目的:研究妊娠期高血压疾病患者血清胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-1(IGFBP-1)的水平与病情程度及新生儿出生体重之间的关系。方法:采用放射免疫方法测定并比较38例妊娠期高血压疾病患者与38例正常血压妊娠妇女的血清IGF-1、IGFBP-1的水平。结果:子痫前期组IGF-1显著低于妊娠期高血压组和正常组,而IGFBP-1水平显著高于妊娠期高血压组和正常组;妊娠期高血压组与正常组间IGF-1、IGFBP-1的水平比较,差异均无统计学意义。IGF-1水平与收缩压、舒张压及平均动脉压呈显著负相关,与新生儿出生体重呈显著正相关,而IGFBP-1与收缩压、舒张压及平均动脉压呈显著正相关,与新生儿出生体重呈显著负相关。结论:妊娠期高血压疾病患者的发病及严重程度与IGF-1、IGFBP-1有明显的关系,IGF-1、IGFBP-1与胎儿的发育及新生儿出生体重有明显的相关性。     

血清IGF-1、IGFBP-3水平和大肠癌关系的Meta分析

目的综合评价血清胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)水平和大肠癌的关系。方法利用Meta分析法对6篇关于血清IGF-1、IGFBP-3水平与大肠癌关系的研究文献进行定量综合分析。结果对于IGF-1，合并OR=1．56(95％CI：1．14～2．13)；按实验方法不同分层，间接酶联免疫吸附试验(ELISA)合并OR=1．92(95％CI：1．26～2．93)，IRMA法合并OR=1．23(95％CI：0．78～1．94)；对于IGFBP-3，合并OR=0．78(95％CJ：0．43～1．44)；按实验方法不同分层，ELISA法合并OR=0．46(95％CI：0．29～0．74)，免疫放射测定法(IRMA)合并OR=1．44(95％CI：0．93～2．23)。结论血清IGF-1高水平为大肠癌的独立危险因子，IGFBP-3与大肠癌的关联不具有统计学意义；IGFBP-3与大肠癌关系的各研究之间异质性是由实验方法不同而引起，但该结论尚需大样本并同时进行两种方法的测量证实。     

Low-fat diet with omega-3 fatty acids increases plasma insulin-like growth factor concentration in healthy postmenopausal women

The insulin-like growth factor pathway plays a central role in the normal and abnormal growth of tissues; however, nutritional determinants of insulin-like growth factor I (IGF-I) and its binding proteins in healthy individuals are not well defined. Three test diets—high-fat diet (40% energy as fat), low-fat diet (LF; 20% energy as fat), and a diet with low fat and high omega-3 fatty acid (LFn3; 23% energy as fat)—were tested in a randomized crossover designed controlled feeding trial in healthy postmenopausal women. Plasma IGF-I, IGF binding protein-3 (IGFBP-3), insulin, glucose, and ratio of IGF-I/IGFBP-3 concentrations were measured in response to diets. Insulin sensitivity was calculated using the homeostatic model assessment of insulin resistance We hypothesized that IGF-I, insulin, and glucose concentrations would decrease and IGFBP-3 concentration would increase in response to the low-fat diets. Eight weeks of the LFn3 diet increased circulating IGF-I (P < .001) and IGFBP-3 (P = .01) and the LF diet increased IGFBP-3 (P = .04), resulting in trends toward an increased IGF-I/IGFBP-3 ratio with the LFn3 diet and a decreased IGF-I/IGFBP-3 ratio with the LF diet (P = .13 for both comparisons). No statistically significant differences were detected between treatments at baseline or 8 weeks for IGF-1, IGFBP-3, or the ratio of IGF-1/IGFBP-3. Insulin, glucose, and the homeostatic model assessment of insulin resistance were not altered by the interventions. Low-fat diet with high n-3 fatty acids may increase circulating IGF-I concentrations without adversely affecting insulin sensitivity in healthy individuals.     

Loss of hip bone mineral density over time is associated with spine and hip fracture incidence in osteoporotic postmenopausal women

The objective of the study assess the relationship between bone mineral density (BMD) loss over time and fracture incidence in postmenopausal women. This is a posthoc analysis that includes women from the placebo group of two large randomized controlled trials having assessed the efficacy of a new anti-osteoporotic drug. BMD was assessed every 6 months during 3 years at the lumbar spine, the femoral neck and the total proximal femur. Vertebral fractures were assessed using a semiquantitative method. Hip fractures were based on written documentation. All patients received calcium and vitamin D. In the present study that included 1,775 patients (with complete data at baseline and after 3 years), the logistic regression analysis, adjusted for covariates, showed that 3-year change in lumbar BMD was not statistically associated with the new vertebral fractures after 3 years. However, femoral neck and total proximal femur BMD changes was statistically correlated with the incidence of new vertebral fractures (P < 0.001). When considering change in BMD after the first year of follow-up, a decrease in total proximal femur BMD was statistically associated with an increase in the incidence of new vertebral fractures during the last 2 years of follow-up (P = 0.048). The 3-year change in femoral neck and total proximal BMD was statistically correlated with the incidence of hip and fragility fracture after 3 years (all P < 0.001). In this elderly osteoporotic population receiving calcium and vitamin D, a decrease in hip BMD after 1 or 3 year of follow-up, is associated with an increased risk of fracture incidence. However, spine BMD changes do not influence vertebral fracture incidence.     

大豆异黄酮和补充钙对去卵巢大鼠骨密度及肝IGF-1mRNA表达的影响

目的 利用卵巢切除大鼠模型,研究大豆异黄酮和钙对卵巢切除大鼠骨密度及肝脏IGF-1基因表达的影响.方法 将6月龄雌性SD大鼠,按体重随机分成5组假手术组(Sham)、卵巢切除阴性对照组(OVX)、单纯大豆异黄酮组(SI)、单纯碳酸钙组(Ca)、大豆异黄酮加碳酸钙组(SI+Ca).所有大鼠饲以钙含量为3.732g/kg的低钙饮食喂养12周.实验结束时,利用双能X线骨密度扫描仪测量右侧股骨骨密度(BMD),采用逆转录-聚合酶链反应(RT-PCR)方法测定肝脏胰岛素样生长因子-1(IGF-1)基因表达水平.结果 SI+Ca组股骨远心端BMD为(0.263±0.007)g/cm2,Sham组为(0.267±0.008)g/cm2,两组间差异无统计学意义,但这两组BMD均显著性高于OVX(0.245±0.005)g/cm2、SI(0.258±0.011)g/cm2和Ca(0.255±0.004)g/cm2组(P＜0.05).肝脏IGF-1mRNA表达水平在Sham(0.200±0.023)g/cm2、SI(0.278±0.019)g/cm2、Ca(0.302±0.026)g/cm2及SI+Ca(0.231±0.025)g/cm2组中均显著性低于OVX(0.362±0.031)g/cm2,P＜0.05,SI+Ca组IGF-1mRNA表达水平(0.231±0.025)g/cm2低于SI(0.278±0.019)g/cm2和Ca(0.302±0.026)g/cm2两组,并差异有统计学意义.结论 SI+Ca能比单纯喂饲SI或Ca更好地防止卵巢切除大鼠股骨BMD的减小.37.95 mg/kg剂量的SI能够显著抑制由于卵巢切除引起的肝脏IGF-1 mRNA表达水平的上升.     

Prognosis of osteopenia in chronic alcoholics

Osteoporosis is frequent among alcoholics all by a direct effect of ethanol, malnutrition, and liver failure. Therefore, it may be related to survival. The aim of this study was to assess bone mineral density (BMD), bone mineral content, hormonal status, and to determine prognostic value of these parameters in a total of 124 alcoholics followed up for a median period of 57 months. Several bone homeostasis-related hormones were measured in patients and age- and sex-matched controls. Whole-body densitometry was performed by a Hologic QDR-2000 (Waltham, MA) densitometer; nutritional status and liver function were assessed. Sixty patients underwent a second evaluation 6 months later. Patients showed lower serum insulin-like growth factor-1 (median = 58, interquartile range [IQR] = 33-135 vs. 135 ng/mL, IQR = 116-243 ng/mL, P < .001), vitamin D (25.5, IQR = 18.3-36.8 vs. 79.9 pg/mL, IQR = 59.2-107.8 pg/mL, P < .001), and osteocalcin (2.1, IQR = 1.1-4.5 vs. 6.5 ng/mL, IQR = 4.7-8.7 ng/mL, P < .001) than controls, and lower BMD values, and lower Z- and T-scores at right and left legs and arms, thoracic and lumbar spine, pelvis, and right and left ribs. By multiple regression analysis, BMD mainly depends on nutritional parameters and liver function. Kaplan-Meier curves show that subtotal BMD and BMD at both arms and pelvis were significantly related with survival. Patients who had lost total hip BMD after 6 months showed a shorter survival than those who had not, but using Cox’s regression, encephalopathy, ascites, and nutritional parameters displaced BMD as prognostic factor. Therefore, osteopenia ensues in chronic alcoholic patients. It mainly depends on poor nutrition and is related to survival, although surpassed in this sense by encephalopathy, ascites, and nutritional parameters.     

非营养性吸吮对早产儿血清IGF-1、IGFBP-3及生长发育的影响

目的:探讨非营养性吸吮对早产儿血清IGF-1、IGFBP-3及生长发育的影响。方法:以2008年9月～2009年8月收治的早产儿60例为研究对象,随机分为非营养性吸吮(NNS)组和对照组,采用ELISA法测定生后第1天开奶前及生后第3天、第7天、第14天血清IGF-1、IGFBP-3水平,同时记录生长发育指标(头围、身长)。结果:①NNS组血清IGF-1、IGFBP-3水平在生后第7、14天高于对照组(P<0.05)。②与对照组相比,NNS组第14天头围、体重增长差异有统计学意义(P<0.01)。③血清IGF-1与头围、体重增长呈正相关(r=0.684,P<0.01;r=0.656,P<0.01),与血清IGFBP-3水平呈正相关(r=0.659,P<0.01)。结论:NNS能提高血清IGF-1、IGFBP-3水平,加快早产儿的生长发育。     

老年腹腔镜手术患者胰岛素样生长因子、血尿酸水平与患者认知能力的关系

目的探讨老年腹腔镜患者血清中胰岛素样生长因子(IGF-1)、尿酸(UA)水平与患者认知能力的关系。方法选择2017年5月-2018年5月在本院行腹腔镜下胆囊切除手术治疗的老年患者90例,所有患者均采用酶联免疫吸附法检测血清中IGF-1,并检测患者UA水平,记录患者生活质量和自我效能、认知功能的关系,采用相关性分析探讨血清中IGF-A、UA水平与患者临床特点的关系。结果IGF-1高水平、UA高水平的患者自我效能和生存质量评分明显高于对应低水平的患者(P<0.05);IGF-1低水平的患者MMSE和LOTCA评分均高于对应高水平的患者(P<0.05),UA低水平的患者自我效能和生存质量评分均低于对于高水平的患者(P<0.05);UA与自我效能、生活质量、MMSE、LOTCA评分呈正相关(P<0.05);IGF-1与自我效能、生活质量呈正相关(P<0.05),与MMSE、LOTCA评分呈负相关(P<0.05)。结论老年腹腔镜手术患者胰岛素样生长因子、血尿酸水平与患者认知功能有关。     

肝细胞癌合并肝硬化患者癌组织、癌旁肝组织ALB mRNA、IGF-1 mRNA、IGFBP-3 mRNA的表达

目的探讨肝细胞癌合并肝硬化患者癌组织、癌旁肝组织ALB mRNA、IGF-1mRNA、IGFBP-3mRNA的表达。方法对24例肝细胞癌合并肝硬化行手术的患者和12例因胆石症或肝血管瘤行手术的患者进行研究。用RT-PCR法检测肝组织(包括癌组织、癌旁组织)ALBmRNA、IGF-1mRNA、IGFBP-3mRNA的表达,肝组织行Ki67免疫组织化染色。结果肝细胞癌合并肝硬化患者癌组织、癌旁肝组织ALB mRNA、IGF-1mRNA、IGFBP-3mRNA表达水平明显低于正常肝组织,且癌组织ALB mRNA、IGFBP-3mR-NA表达水平明显低于癌旁肝组织,而IGF-1mRNA表达水平明显高于癌旁肝组织。肝组织Ki67指数,癌组织、癌旁肝组织明显高于正常肝组织,且癌组织明显高于癌旁肝组织。结论IGF-1和IGFBP-3的不平衡可能与肝硬化患者肝癌的发生、发展有关。     

Binge alcohol treatment of adolescent rats followed by alcohol abstinence is associated with site-specific differences in bone loss and incomplete recovery of bone mass and strength

We previously demonstrated that alcohol-fed adolescent rats exhibit reductions in lumbar spine bone mineral density (BMD) and vertebral body height, suggesting that chronic alcohol consumption has negative consequences for skeletal development during adolescence. Binge alcohol consumption is common in adolescents and young adults, yet little is known about its consequences on skeletal integrity or the attainment of peak bone mass. We used a previously validated binge alcohol exposure model to test the hypothesis that binge alcohol treatment of adolescent rats would be associated with distinct temporal and site-specific bone loss profiles, with incomplete recovery from bone loss following a period of alcohol abstinence. Seventy-two male adolescent Sprague-Dawley rats were assigned to one of six treatment groups (n = 12/group) receiving binge alcohol (3 g/kg) or saline intraperitoneal, 3 consecutive days (acute binge), 4 consecutive weekly (3-day) binge cycles (chronic binge), or 4 weekly binge cycles followed by a 30-day abstinence period without alcohol or saline injections (chronic binge with abstinence). Cancellous BMD was determined by quantitative computed tomography and compressive strength determined by biomechanical testing. Serum testosterone and osteocalcin levels were measured by enzyme-linked immunosorbent assay. Tibial cancellous BMD was significantly reduced by 25% (P < .05) after both acute and chronic binge alcohol treatment and vertebral cancellous BMD was significantly reduced by 15% (P < .05) after chronic binge exposure. Vertebral compressive strength was also significantly decreased by 31% (P < .05) after chronic binge alcohol treatment. Tibial cancellous BMD returned to control levels after the 30-day alcohol abstinence period, but vertebral cancellous BMD remained 15% below control values (P < .05) 30 days after termination of binge alcohol exposures. Serum osteocalcin levels were significantly decreased following acute binge alcohol exposure (P < .05). These results show that binge alcohol exposure can produce both short- and long-term skeletal damage in the adolescent rat. These data might have relevance to peak bone mass attainment and future risk of skeletal disease in adolescents and young adults who engage in repeated binge-drinking episodes.     

母亲妊娠合并糖尿病对新生儿血糖、胰岛素、胰岛素样生长因子-1的影响

目的:研究母亲妊娠合并糖尿病对新生儿血糖、胰岛素、胰岛素样生长因子-1(IGF-1)的影响。方法:29例母亲妊娠合并糖尿病的新生儿及20例健康新生儿均于生后1h内测空腹血糖,并采脐血用放射免疫法测定胰岛素、IGF-1水平。结果:母亲妊娠合并糖尿病其新生儿生后血糖水平低于对照组(P<0.05);胰岛素、IGF-1水平高于对照组(P<0.05),血糖水平与IGF-1呈负相关(r=-0.432,P<0.05)。结论:母亲妊娠合并糖尿病会导致新生儿生后血糖水平的降低,胰岛素、IGF-1水平的升高,二者共同参与血糖的调节,对这部分新生儿应做好血糖监测,防止低血糖的发生。     

Relation between plasma brain-derived neurotrophic factor and nerve growth factor in the male patients with alcohol dependence

Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) are thought to be related to neuroprotection in cell culture and animal studies. Our aim was to verify the changes in human plasma BDNF and NGF concentrations induced by chronic alcohol use. Forty-one male patients with alcohol dependence were sampled the next morning of admission and compared with 41 healthy male subjects. Plasma BDNF and NGF were assayed using an enzyme-linked immunosorbent assay (ELISA). Mean plasma BDNF level was significantly higher in the patients with alcohol dependence (3502.21 ± 1726.9 pg/mL) compared with the healthy subjects (861.75 ± 478.9 pg/mL) (P = .000). Mean plasma NGF level was also significantly higher in patients with alcohol dependence (137.64 ± 32.7 pg/mL) than in healthy subjects (112.61 ± 90.2 pg/mL) (P = .012). Plasma BDNF and NGF levels showed significant negative correlation in alcohol dependence group (r = −0.388, P = .012). Increased plasma BDNF and NGF with negative correlation in alcohol-dependent patients may have some role in the regeneration of damage done by chronic alcohol use.     

Excess body fat negatively affects bone mass in adolescents

ObjectiveThe aim of this study was to investigate the effects of excess body fat on bone mass in overweight, obese, and extremely obese adolescents.MethodsThis study included 377 adolescents of both sexes, ages 10 to 19 y. Weight, height, body mass index (BMI), bone age, bone mineral content (BMC), and bone mineral density (BMD) were obtained by dual-energy x-ray absorptiometry. The results were adjusted for chronological age and bone age. Comparisons according to nutritional classification were performed by analysis of variance, followed by Tukey test. Linear regression models were used to explain the variation in BMD and BMC in the L1–L4 lumbar spinal region, proximal femur, and whole body in relation to BMI, lean mass, fat mass (FM), and body fat percentage (BF%), considering P < 0.05.ResultsFor all nutritional groups, average bone age was higher than chronological age. In both sexes, weight and BMI values increased from eutrophic to extremely obese groups, except for BMD and BMC, which did not differ among male adolescents, and were smaller in extremely obese than in obese female adolescents (P < 0.01). Significant differences were observed for FM and BF% values among all nutritional groups (P < 0.01). Positive, moderate to strong correlations were detected between BMD and BMC for BMI, lean mass, and FM. A negative and moderate correlation was found between BMC and BF%, and between BMD and BF% at all bone sites analyzed in males and between BF% and spine and femur BMD, in females.ConclusionThe results reveal a negative effect of BF% on bone mass in males and indicate that the higher the BF% among overweight adolescents, the lower the BMD and BMC values.     

IGF-1/IGFBP-1与妊娠期糖尿病的相关性

目的:探讨妊娠24～28周孕妇外周IGF-1/IGFBP-1与GDM的相关性,为寻找早期预测GDM的生物学指标提供前期研究。方法:妊娠24～28周GDM孕妇35例为实验组,同期NGT孕妇30例为对照组。采用酶联免疫吸附法检测孕妇外周血IGF-1及IGFBP-1表达水平。结果:①实验组血清IGF-1水平较对照组低,差异有统计学意义(t=-5.71,P<0.05),实验组血清IGFBP-1水平较对照组高,差异有统计学意义(t=2.60,P<0.01)。②IGF-1与IGFBP-1成负相关(r=-0.559 85,P<0.000 1)。结论:孕妇外周血IGF-1/IGFBP-1是调节血糖的重要因素,其平衡失调是介导IR导致GDM患者糖耐量异常的重要机制。     

Markers of fetal growth and serum levels of insulin-like growth factor (IGF) I, -II and IGF binding protein 3 in adults

Fetal growth has been linked with increased risk of cancer and cardiovascular disease later in life. The insulin-like growth factor (IGF) axis has recently been proposed as a predictor of risk of subsequent cancer and cardiovascular disease. However, only few data are available on the possible association between fetal growth and levels of IGFs later in life. We examined the association between markers of fetal growth, i.e. birth weight, birth length and Ponderal Index, from birth records and serum IGF-I, IGF-II, and IGF binding protein 3 (IGFBP-3) levels in 545 middle-aged Danish men and women. We fitted separate multivariate models including birth weight, birth length, Ponderal Index and serum IGF-I, IGF-II, and IGFBP-3, respectively. After adjustment for age, alcohol intake, smoking, diabetes mellitus, systolic and diastolic blood pressure, serum total cholesterol and current height and weight, we found negative associations between birth weight and Ponderal Index, respectively, and serum IGF-II in men, i.e. the mean regression coefficients were -49.41 (95% CI: -87.06-11.77) (microg/l)/kg and -3.49 (95% CI: -6.73-0.25) (microg/l)/(kg/m3), respectively. Furthermore, in men birth weight was negatively associated with the (IGF-I + IGF-II)/IGFBP-3 and IGF-II/IGFBP-3 ratios, which are believed to be indicators of bioavailable IGF and IGF-II, respectively. However, no other associations were found in any of the models. Between 1 and 16% of the variance in serum IGF-I, IGF-II, and IGFBP-3, respectively, could be explained by the statistical models used in the analyses. We found very little support to the hypothesis of an association between fetal growth and the IGF axis throughout life.     

肝细胞癌合并肝硬化患者术后应用重组人生长激素联合肠外营养对生长激素/胰岛素样生长因子-1轴的影响

目的：探讨肝细胞癌合并肝硬化患者术后应用重组人生长激素（rhGH）联合肠外营养（PN）对生长激素（GH）／胰岛素样生长因子-1（IGF-1）轴的影响。方法：24例行根治性切除的肝癌患者随机分为PN组（n=12）和rhGH＋PN组（n=12）。术前、术后1d和6d测血清GH、IGF-1、IGFBP-3，用RT-PCR法检测肝组织（包括癌组织，癌旁组织和术后6d肝穿刺组织）IGF-1 mR-NA、IGFBP-3 mRNA的表达，肝组织行Ki67免疫组织化学染色。同时选12例因胆石症或肝血管瘤行手术的患者作为正常对照。结果肝细胞癌合并肝硬化患者血清GH水平高于正常对照组，而血清IGF-1、IGFBP-3水平低于正常对照组；术后6d，血清GH、IGF-1、IGFBP-3水平、肝组织IGF-1 mRNA、IGFBP-3m RNA表达水平、肝Ki67指数，rhGH＋PN组均高于PN组。PN组中位无瘤生存期是11．2个月，rhGH＋PN组中位无瘤生存期是11．4个月；PN组6个月、12个月无瘤生存率分别是71．1％、46.2％，rhGH＋PN组6个月、12个月无瘤生存率分别是72．2％、59、0％，差异均无统计学意义（P〉0.05）。结论：rhGH＋PN有利于改善肝细胞癌合并肝硬化患者术后GH／IGF-1轴。     

IGF-1对体外培养囊胚细胞抗凋亡的作用

目的:探讨胰岛素样生长因子-1(IGF-1)对体外培养囊胚细胞抗凋亡的作用。方法:获取妊娠3.5天小鼠囊胚,分别移入3个培养皿中,为A、B、C 3组,A组(基础培养液)、B组(基础培养液+30 mmol/L的葡萄糖溶液)、C组(基础培养液+30 mmol/L的葡萄糖溶液+100 ng/ml的人重组IGF-1)。连续培养72 h后,采用免疫组化S-P法检测各组囊胚细胞中Bax和Bcl-2的表达,利用计算机图像分析技术测量各组囊胚细胞中Bax和Bcl-2蛋白表达的平均光密度和平均阳性面积。结果:B组囊胚细胞中Bax的表达明显高于A组和C组(P<0.05),而B组囊胚细胞中Bcl-2的表达却低于A组和C组(P<0.05)。结论:IGF-1对高糖诱导的小鼠着床前早期胚胎凋亡起了抑制作用。     

Bone mineral density and the risk of breast cancer: a case-control study of Korean women

PurposeBone mineral density (BMD) may be useful as a surrogate marker reflecting lifetime exposure to estrogen in a woman. Our study aimed to investigate an association between BMD and breast cancer risk.MethodsA case-control study was conducted using 253 breast cancer cases and 506 age and menopausal status–matched controls from the same institution. Cases were ascertained through medical record review of the women with abnormal mammographic findings. BMD was measured at the lumbar spine and femoral neck using a dual-energy X-ray absorptiometry. The association was estimated by conditional logistic regression analysis with an adjustment for covariates.ResultsAlthough there was no difference in the association between pre- and postmenopausal disease, the association between BMD and breast cancer was evident for postmenopausal breast cancer. One standard deviation in age and menopausal status adjusted BMD at lumbar spine and femur neck was associated with 1.35-fold (standard error = 0.19, P = .04) and 1.34-fold (standard error = 0.20, P = .05) increased likelihood of breast cancer risk, respectively, for postmenopausal women.ConclusionAfter adjusting for covariates, higher BMD at lumbar spine and femur neck are associated with increased likelihood of breast cancer risk for postmenopausal women. These findings suggest that BMD could be included in breast cancer risk prediction models for postmenopausal Korean women.     

Compromised Bone Health in Non-Obese,Older Women with Low Caloric Intake

Low calorie diets are often employed to reverse obesity and lower the risk of other diseases. Further, to maintain a certain physique and/or minimize the incidence of obesity, normal weight, older women may consume a diet low in calories. However, a low caloric intake can potentially compromise bone formation elevating the risk of osteoporosis. Thus, the purpose of this study was to compare the bone mineral density (BMD) from non-obese, peri- and postmenopausal women who were placed into one of three groups based upon their daily caloric intake to determine if bone health was compromised when caloric intake was low. Women (age range 44–80 years old) with a body mass index <25 were separated into one of three groups based upon a dietary analysis to assess nutritional intake: those who consumed 100% of the recommended minimum daily requirement (RMDR, n = 21), 80% RMDR (n = 16), or 55% RMDR (n = 15). BMD was determined via dual energy X-ray absorptiometer from the lumbar spine and femur (left & right). The 55% RMDR group demonstrated significantly lower femoral BMD (~10% lower, P < 0.05) and a trend toward lower BMD of the spine compared to both the 80% RMDR (P = 0.13) and 100% RMDR groups (P = 0.15). There were no significant differences in BMD between the 80% RMDR and 100% RMDR groups. The results suggest that bone health was maintained with moderately low caloric intake (i.e. 80%RMDR) whereas extremely low caloric intake (i.e. 55% RMDR) can compromise bone health in non-obese, older women.