共查询到20条相似文献,搜索用时 31 毫秒
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Specific protein binding to far upstream activating sequences in polymerase II promoters. 总被引:79,自引:11,他引:79 下载免费PDF全文
R J Bram R D Kornberg 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(1):43-47
A binding activity specific for the upstream activating sequence of the GAL1-GAL10 promoter of Saccharomyces cerevisiae has been purified 220-fold on the basis of a nitrocellulose filter-binding assay. The binding activity is enriched in a nuclear preparation and is likely to be the GAL4 gene product. DNase I-protection mapping patterns reveal binding to two 30-base-pair regions at the boundaries of the sequence. A nearly identical mapping pattern is obtained with the coordinately regulated GAL7 promoter. The four 30-base-pair regions of binding in the two promoters are closely homologous, with a core consensus sequence of C-G-CG-TG-C-A-A-C-A-G-T-G-C-T-C-C-G-A-A- GC-G-A-T. A synthetic oligonucleotide with such a sequence competes with the upstream activating sequence in the binding reaction. 相似文献
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Mammalian cAMP-responsive element can activate transcription in yeast and binds a yeast factor(s) that resembles the mammalian transcription factor ANF 总被引:12,自引:2,他引:12 下载免费PDF全文
R H Jones N C Jones 《Proceedings of the National Academy of Sciences of the United States of America》1989,86(7):2176-2180
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Yeast Gal11 protein mediates the transcriptional activation signal of two different transacting factors, Gal4 and general regulatory factor I/repressor/activator site binding protein 1/translation upstream factor 总被引:18,自引:3,他引:18 下载免费PDF全文
M Nishizawa Y Suzuki Y Nogi K Matsumoto T Fukasawa 《Proceedings of the National Academy of Sciences of the United States of America》1990,87(14):5373-5377
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