Influence of interleukin-6 and G174C polymorphism in IL-6 gene on obesity and energy balance

Obesity is a multifactor disease with a very complicated etiology. Genetic factors play an important role in the development of primary obesity. They may be responsible for up to 40% of causes leading to obesity. There are a great number of genes affecting food intake and energy expenditure. Serious consequences accompanying obesity, e.g., type 2 diabetes and lipid abnormalities may be caused by increased level of proinflammatory cytokines, such as IL-1, IL-6, and TNF. It is possible that polymorphisms located in cytokine genes affect the level of protein expression. It is known that IL-6 plays a role in lipid metabolism and energy expenditure. The polymorphism found in point 174 (G174C) of a promoter region of IL-6 gene affects the level of interleukin-6 expression and, consequently, may lead to obesity and correlated conditions.     

血清PCT、IL-6及CRP水平对急性缺血性脑卒中患者神经损伤的影响

目的 探究降钙素原（procalcitonin,PCT）、白细胞介素（interleukin,IL）-6及C反应蛋白（C-reactive protein,CRP）水平对急性缺血性脑卒中（acute ischemic stroke,AIS）患者神经损伤的影响。方法 选取2021年1月至2021年12月东阳市横店医院收治的80例AIS患者作为AIS组,选取同期接受体检并确定至少有1种脑血管疾病相关危险因素的80例体检者作为对照组。比较两组的血清PCT、IL-6及CRP水平。根据美国国立卫生研究院卒中量表（National Institutes of Health stroke scale,NIHSS）评分,将AIS组分为重度脑卒中组（n=23）,中度脑卒中组（n=32）与轻度脑卒中组（n=25）,比较3组的PCT、IL-6及CRP水平。结果 AIS组患者血清PCT、IL-6及CRP水平均高于对照组,差异均有统计学意义（P<0.05）。与轻度脑卒中组相比,中度脑卒中组及重度脑卒中组的血清PCT、IL-6及CRP水平均升高,差异均有统计学意义（P<0.05）。与中度脑卒中组相比,...     

T细胞受体稳定区α基因链+1592C/T多态性与系膜增生性肾炎临床病理的关联分析

目的 探讨TCRCα基因+1592C/T多态性与中国汉族人群系膜增生性肾炎的相关关系.方法 用PCR-RFLP法和PCR产物直接测序法鉴定244例我国汉族人群系膜增生性肾炎患者的基因型,按基因型分为CC(188人)、CT(54人)、TT(2人)3组,采用临床、病理资料分析的方法.结果 临床分析显示:TCRCα+1592C/T三种基因型的系膜增生性肾炎患者的年龄、血压、24h尿蛋白、血清肌酐水平、C-反应蛋白、血沉方面的临床指标差异均无统计学意义(P＞0.05);TCRCα+1592C/T三种基因型的系膜增生性肾炎患者的血压、血脂、血清IgA,C3,CA水平方面的临床指标差异均无统计学意义(P＞0.05);病理资料显示:TCRCα+1592C/T三种基因型的系膜增生性肾炎患者的小球指数、硬化指数、新月体数、间质指数、血管指数的肾脏病理指标方面,差异均无统计学意义(P＞0.05).结论 中国汉族人群中,TCRCα基因+1592C/T多态性与系膜增生性肾炎的临床及病理无关联关系,TCRCα基因+1592C/T可能与系膜增生性肾炎的疾病发生不相关.     

老年高血压病患者脂联素与白细胞介素-6、超敏C反应蛋白的相关性研究

目的探讨老年高血压病患者血清脂联素(APN)与白细胞介素-6(IL-6)、超敏C反应蛋白(hs-CRP)的相关性。方法选择老年高血压病患者(高血压病组64例,其中高血压1级亚组23例,2级亚组21例,3级亚组20例)。老年健康体检者(健康对照组25例),采用酶联免疫法测定APN、IL-6水平,免疫比浊法测定hs-CRP水平。结果高血压病组APN浓度[(7.8±5.6)mg/L]低于健康对照组[(9.9±3.3)mg/L](P<0.05),而IL-6水平[(59.9±7.5)ng/ml]、hs-CRP水平[(5.9±2.0)mg/L]高于健康对照组[分别为(18.7±5.4)ng/ml和(2.4±1.4)mg/L],差异均有统计学意义(P<0.05);且高血压2级、3级亚组与1级亚组比较差异亦有统计学意义(P<0.05)。相关性分析表明,APN与IL-6、hs-CRP呈负相关(分别为r=-0.577,P<0.05;r=-0.582,P<0.05)。结论老年高血压病患者的APN降低,IL-6、hs-CRP升高,APN与IL-6、hs-CRP呈负相关。     

The predictors of subclinical cerebral infarcts in ischaemic stroke patients

Subclinical cerebral infarcts (SCI) have been increasingly shown to cause a significant clinical impact. However, there are limited data available on Asian patients. The objectives of this study were to determine the prevalence of SCI in ischaemic stroke patients seen in the Hospital Universiti Kebangsaan Malaysia (HUKM) and to identify its associated risk factors. We evaluated the brain computed tomography (CT) evidence of subclinical infarcts in ischaemic stroke patients. The patients were selected from those who were on regular follow up in the neurology clinic following an ischaemic stroke. The risk factors associated with subclinical infarct were documented. The brain CTs were done during acute admission and reviewed for SCI. Sixty-one patients were enrolled in this study. Twenty-two (36.1%) out of the 61 patients had SCI. The risk factors for SCI in our study were hypertension (OR 14.16 CI 2.04-97.89), diabetes mellitus (OR 12.48; CI 1.95-79.77) and leukoaraiosis (OR 28.39; CI 2.33-284.16). Subclinical cerebral infarcts were present in about one third of our ischaemic stroke patients. This finding is higher than in previous studies done on Caucasians. Hypertension, diabetes mellitus and leukoaraiosis independently predict SCI.     

IL-6-174G/C及-572C/G位点基因多态性与新疆NVAF人群及民族差异性分析

目的 探讨新疆地区白细胞介素-6(IL-6)-174C/G及-572C/G位点的基因多态性,与非瓣膜性心房颤动(NVAF)的关系及新疆地区汉族、哈萨克族(哈族)IL-6基因多态性的民族差异性.方法 分别选择新疆地区汉族、哈族NVAF对象79、78铡(汉族、哈族NVAF组),汉族、哈族非心房颤动对象75、79例(汉族、哈族对照组),采集静脉血提取DNA,采用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)技术对各组对象的IL-6基因-174G/C及-572C/G的多态性进行分析.结果 IL-6-572G在NVAF组中等位基因频率明显高于对照组(x2=4.076,P＜0.05);NVAF组和对照组IL-6基因-572G/C位点G等位基因频率比较,差异有统计学意义(OR=1.519,95％CI:1.087～2.122,P＜0.05),而在汉族及哈族对照组中分布差异无统计学意义(P＞0.05);年龄大于或等于75岁(OR=6.468,95％CI:2.427～17.240)、左心房内径(OR=1.053,95％CI:1.022～1.085),是发生房颤的独立危险因素;左心室射血分数(OR=0.946,95％CI:0.922～0.971)是防止房颤发生的保护因素.各组IL-6基因-174G/C位点C、G等位基因频率比较,差异无统计学意义(P＞0.05).结论 IL-6-572G/C位点G等位基因是NVAF遗传易感性的危险因子,IL-6-174G/C位点的基因多态性与心房颤动的易感性无关.     

H型高血压与早发性脑梗死颈动脉内膜中层厚度及相关因素的研究

目的探讨早发性脑梗死患者中H型高血压和颈动脉内膜中层厚度及相关因素的关系。方法选取162例早发性脑梗死患者,根据血压、同型半胱氨酸(Hcy)水平分为H型高血压组(Hcy≥10μmol/L)、单纯高血压组(Hcy<10μmol/L)、非高血压组,比较三组患者的临床资料、生化指标及颈动脉内膜中层厚度(CIMT)。结果 H型高血压组与单纯高血压组收缩压(SBP)、舒张压(DBP)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、CIMT高于非高血压组,差异有统计学意义(P<0.05或0.01);H型高血压组CIMT高于单纯高血压组,差异有统计学意义;相关分析显示在H型高血压组Hcy与CIMT呈正相关,单纯高血压组则无相关;H型高血压组资料经Spearman相关分析显示,CIMT与年龄、TC、TG、LDL-C呈正相关,与高密度脂蛋白胆固醇(HDL-C)呈负相关;Logistic多元回归分析显示,HDL-C、Hcy是H型高血压组颈动脉内膜增厚的独立危险因素。结论 Hcy是H型高血压颈动脉内膜增厚的独立危险因素,控制"H型高血压"是早发性脑梗死防治的有效措施。     

缺血性卒中患者ApoA5-1131T/C基因多态性研究

目的 分析缺血性卒中患者ApoA5-1131T/C基因单核苷酸多态性、各等位基因、基因型分布频率及其与血清甘油三酯水平的相关性,探讨ApoA5基因多态性与缺血性卒中的关系.方法 选择缺血性卒中患者140例,另设健康对照组129例;用聚合酶链反应限制性片段长度多态性分析-1131T/C位点多态性,并检测血脂水平.结果 缺血性卒中组-1131C等位基因频率高于对照组(P=0.008);缺血性卒中组和对照组稀有等位基因-1131C携带者甘油三酯水平均高于非携带者(t=3.410,P=0.001和t=5.741,P=0.000).校正年龄、性别、胆固醇、高血压、糖尿病、吸烟、血脂因素后,Logistic回归分析显示携带-1131C等位基因是缺血性卒中的独立危险因素(P=0.004,95% CI:1.383～5.255).结论 对于心源性脑栓塞和大动脉粥样硬化性卒中患者,-1131C等位基因与血清甘油三酯水平升高及缺血性卒中易感性有关.     

缺血性卒中患者ApoA5-1131T/C基因多态性研究

目的 分析缺血性卒中患者ApoA5-1131T/C基因单核苷酸多态性、各等位基因、基因型分布频率及其与血清甘油三酯水平的相关性,探讨ApoA5基因多态性与缺血性卒中的关系.方法 选择缺血性卒中患者140例,另设健康对照组129例;用聚合酶链反应限制性片段长度多态性分析-1131T/C位点多态性,并检测血脂水平.结果 缺血性卒中组-1131C等位基因频率高于对照组(P=0.008);缺血性卒中组和对照组稀有等位基因-1131C携带者甘油三酯水平均高于非携带者(t=3.410,P=0.001和t=5.741,P=0.000).校正年龄、性别、胆固醇、高血压、糖尿病、吸烟、血脂因素后,Logistic回归分析显示携带-1131C等位基因是缺血性卒中的独立危险因素(P=0.004,95% CI:1.383～5.255).结论 对于心源性脑栓塞和大动脉粥样硬化性卒中患者,-1131C等位基因与血清甘油三酯水平升高及缺血性卒中易感性有关.     

377例无神经系统症状原发性高血压患者脑卒中发病高危因素及CT结果分析

目的:研究无神经系统症状原发性高血压患者中脑卒中的发病率及其影响因素,并研究其CT检查的意义。方法:回顾性总结分析377例无神经系统症状原发性高血压患者的相关临床资料,分析无神经系统症状原发性高血压患者脑卒中的发病率及其与高血压分级、年龄、性别、饮酒、吸烟、肥胖、糖尿病、血脂异常等相关因素的关系,并分析CT对其的诊断价值。结果:377例无神经系统症状原发性高血压患者中经CT检查发现脑卒中患者81例(21.48%),高血压Ⅲ级患者中的患病率高于高血压Ⅰ、Ⅱ级患者。而年龄≥55岁、男性、饮酒、吸烟、肥胖、糖尿病及血脂异常等患者中的患病率(26.79%、26.79%、28.24%、27.48%、41.11%、57.89%、45.07%)分别高于年龄<55岁、女性、不饮酒、不吸烟、体重正常、无糖尿病、血脂正常的患者(17.85%、14.88%、17.88%、13.85%、15.33%、15%、16.01%)(P<0.05)。结论:无神经系统症状原发性高血压患者脑卒中可以通过CT诊断,特别对于高血压Ⅲ级及相关危险因素患者应积极行CT检查,以早期干预治疗。     

ATP2B1基因多态性与高血压关系的研究进展

原发性高血压作为一种复杂疾病,其致病机理除了环境因素诱因外,遗传占有很大一部分。近年来随着测序技术的飞速发展,全基因组关联分析作为一种高效的统计遗传学手段使人们对多因素疾病背后复杂的遗传因素认识不断加深。现今已知与原发性高血压的关联的基因高达150多种,其中又以ATP2B1最为显著。无论是在欧洲还是东亚,其在不同人种中与原发性高血压的关联都具有显著的统计学意义。在此通过本文来探讨ATP2B1基因多态性与原发性高血压的关系。     

稳定期及发作期慢性阻塞性肺疾病患者痰液白细胞介素-1β和白细胞介素-6变化

目的观察吸烟、不吸烟慢性阻塞性肺疾病(COPD)稳定期及发作期患者痰液白细胞介素-1β(IL-1β)和IL-6水平差异,探讨其与临床症状及肺功能的关系.方法87例中度～重度的COPD患者(吸烟44例,不吸烟43例),记录每日的症状,并在稳定期及发作期分别测定诱导痰中炎症细胞及IL-1β和IL-6浓度的变化.结果稳定期吸烟组患者诱导痰IL 1β为85.1 pg/ml,IL-6为76.5 pg/ml,较不吸烟组的60.1 pg/ml和56.4 pg/ml明显升高(P＜0.05).发作期吸烟组和不吸烟组的IL-1β和IL-6水平均较稳定期升高,吸烟组(IL-1β 323.6 pg/ml,IL-6 106.7pg/ml)比不吸烟组(IL-1β 78.4 pg/ml,IL-6 90.2 pg/ml)升高更明显(P＜0.01).发作期IL-6水平与痰液中细胞总数、嗜酸粒细胞计数和淋巴细胞计数相关.稳定期IL-1β水平与痰液细胞总数、中性粒细胞计数、嗜酸粒细胞计数和淋巴细胞计数相关;IL-1β和IL-6水平与COPD的发作频率相关(r=0.383,P=0.01和r=0.311,P＜0.05),与肺功能及症状积分无关.结论COPD患者痰液中IL-1β和IL-6水平升高,表明气道炎症程度较重,预示COPD急性发作频率较高.     

SLC6A4基因rs2020939多态性与卒中后抑郁相关性的研究

目的探讨SLC6A4基因的单核苷酸多态性位点rs2020939与卒中后抑郁（PSD）的相关性。方法本研究纳入246例首次发生脑梗死的患者,入院时登记患者基本资料、脑梗死病灶、美国国立卫生研究院卒中量表（NIHSS）评分和脑血管病危险因素等,入院时和随访后3个月均采用汉密尔顿抑郁量表（HAMD）进行抑郁评分,根据3个月后HAMD评分将脑梗死患者分成卒中后抑郁组（n=86）和卒中后无抑郁组（n=160）。运用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）技术对rs2020939进行基因分型。用单因素和多因素Logistic回归模型来分析卒中后抑郁的危险因素。结果年龄为卒中后抑郁的保护因素（P=0．0047,OR=0．977,95％可信区间0．955—1．000）,NIHSS评分（P=0．00,OR=1．386,95％可信区间1．249～1．583）与PSD有关。rs2020939多态位点基因型和等位基因在卒中后抑郁组和卒中后无抑郁组中的分布均有显著性差异（基因型：Х^2=10．007,P=0．007;等位基因：Х^2=10．551,P=0．001）。结论除了患者年龄、脑梗死后病情严重程度与PSD有关外,还发现SLC6A4基因rs2020939位点多态性与淮海地区汉族人群卒中后抑郁的发病相关,该位点可能是PSD的易感基因。     

The microsatellite polymorphism of heme oxygenase-1 is associated with baseline plasma IL-6 level but not with restenosis after coronary in-stenting

Background Vascular smooth muscle cells (VSMCs) can express heme-oxygenase (HO), a rate-limiting enzyme in the degradation of heme to bilirubin, ferritin and carbon monoxide (CO). VSMC-derived CO can suppress VSMC proliferation and may serve as an antiproliferation factor. The promoter region of HO-1 shows a polymorphism with different (GT)n repeats that has been reported to differently induce gene expression. The objective of this study was to examine the effect of this variation on the occurrence of restenosis after in-stent treatment in patients with coronary artery disease. Methods Candidates who underwent coronary stent implantation were genotyped for the HO-1 promoter polymorphism using polymerase chain reaction (PCR) and automated DNA capillary sequencer. Serum levels of IL-6 and C-reactive protein (CRP) were obtained at baseline, 24 hours and 48 hours after stenting. The primary end point for the study was angiographic evidence of in-stent restenosis at 6 months. All parameters for evaluation of restenosis were analysed by quantitatve computer-assisted angiographic analysis (QCA). Results One hundred and eighty-seven patients who underwent coronary stent implantation were studied of whom 27.8% showed ≥50% restenosis after 6 months. The distribution of (GT)n repeats of all patients in the promoter region of HO-1 genotype ranged from 22 to 42, with (GT)25 and (GT)32 being the two most common alleles. The allelic repeats were divided into the short class (S) with 29 (GT)n, the middle class (M) with 30-37 (GT)n and the long class (L) with 38 (GT)n. There was no significant difference in the restenosis between the genotype groups or between post operation levels of inflammation markers, but carriers of the S allele (n=120) had 33.3% lower baseline IL-6 compared with non-S carriers (n=67, P=0.0008). Conclusions Although no association was observed between the HO-1 promoter polymorphism and coronary in-stent restenosis following the stent procedure, the association with plasma IL-6 levels suggests that HO-1 S allele might protect from the atherosclerotic inflammatory process.     

The prognostic value of C-reactive protein (CRP) levels in patients with acute ischaemic stroke

Increasing evidence suggests that inflammation plays an important role in the development of both cardiovascular and cerebrovascular events. Recently C-reactive protein (CRP) levels have been reported to be a prognostic factor for cerebrovascular and cardiovascular events. The main objective of the study was to evaluate the prognostic value of CRP levels in a first ever ischaemic stroke at one month. All ischaemic stroke patients who were admitted to Hospital Universiti Kebangsaan Malaysia (HUKM) between May 2002 and July 2002 were eligible for the study. CRP levels were taken within 72 hours after an acute ischaemic stroke. The functional ability was assessed using the Barthel Index (BI) after one month of stroke. During the study period 84 patients were admitted to HUKM with the diagnosis of ischaemic stroke; 49 patients were enrolled and 35 were excluded. Twenty-nine patients (59.2%) had elevated CRP levels (median 1.64+/-3.07 mg/dL, range 0.06 to 16.21 mg/dL). Elevated CRP levels were found to be a predictor of severe functional disability (BI<5) and were also associated with larger infarcts. In conclusion, elevated CRP levels are associated with poorer functional outcome and predict a larger infarct size.     

首发精神分裂症患者血浆IL-6、sIL-6R及IL-13水平研究

目的探讨首发精神分裂症患者血浆白细胞介素-6(IL-6)、可溶性白细胞介素-6受体(sIL-6 R)及白细胞介素-13(IL-13)水平的变化. 方法采用酶联免疫吸附法(ELISA)对30例精神分裂症患者和28例健康对照者血浆IL-6、sIL-6 R及IL-13水平进行检测.结果精神分裂症患者血浆IL-6[(20.18±2.59)pg/ml]及sIL-6 R[(33.87±16.51)pg/ml]水平显著高于对照组(P ＜0.05),而血浆IL-13[(16.15±11.98)pg/ml]水平显著低于对照组[(24.41±18.26)pg/ml](P ＜0.05);以阴性症状为主患者的IL-6[(21.04±1.72)pg/ml]及sIL-6 R[(32.83±15.72)pg/ml]水平均高于阳性组,其中阴性组患者IL-6显著高于阳性组[(18.28±3.22)pg/ml](P ＜0.05),阴性组患者血浆IL-13[(12.72±9.01)pg/ml]水平显著低于阳性组[(19.82±7.83)pg/ml](P ＜0.05);未发现患者组及对照组血浆IL-6、sIL-6 R及IL-13之间的相关性.结论精神分裂症患者存在IL-6、IL-13介导的免疫功能异常,血浆IL-6水平升高、IL-13水平降低可能是阴性症状为主患者的特征性免疫学指标之一.     

首发精神分裂症患者血浆IL-6、sIL-6R及IL-13水平研究

目的 探讨首发精神分裂症患者血浆白细胞介素 6(IL 6)、可溶性白细胞介素 6受体 (sIL6R)及白细胞介素 13(IL 13)水平的变化。方法 采用酶联免疫吸附法 (ELISA)对 30例精神分裂症患者和 28例健康对照者血浆IL 6、sIL 6R及IL 13水平进行检测。结果 精神分裂症患者血浆IL 6 [ ( 20. 18±2. 59)pg/ml]及sIL 6R[ (33. 87±16. 51)pg/ml]水平显著高于对照组 (P<0. 05),而血浆IL 13 [ (16. 15±11. 98)pg/ml]水平显著低于对照组 [ (24. 41±18. 26)pg/ml] (P<0. 05);以阴性症状为主患者的IL 6[ (21. 04±1. 72)pg/ml]及sIL 6R[ (32. 83±15. 72)pg/ml]水平均高于阳性组,其中阴性组患者IL 6显著高于阳性组[ (18. 28±3. 22)pg/ml] (P<0. 05),阴性组患者血浆IL 13[ (12. 72±9. 01)pg/ml]水平显著低于阳性组[ (19. 82±7. 83)pg/ml] (P<0. 05);未发现患者组及对照组血浆IL 6、sIL 6R及IL 13之间的相关性。结论 精神分裂症患者存在IL 6、IL 13介导的免疫功能异常,血浆IL 6水平升高、IL 13水平降低可能是阴性症状为主患者的特征性免疫学指标之一。     

The correlation between self-efficacy and knowledge in elder hypertension with stroke patients

目的 探讨老年高血压合并脑卒中患者自我效能与脑卒中防治知识的水平及其相关性.方法 采用健康行为自我效能量表、脑卒中防治知识问卷,对100例名老年高血压合并脑卒中患者进行调查.结果 患者自我效能得分为(59.75±16.61)分,处于中低水平;患者脑卒中防治知识的掌握得分为(20.73±7.71)分,处于中低水平;自我效能与脑卒中防治知识掌握情况呈正相关(P＜0.01).结论 应提高老年高血压或高血压合并脑卒中患者自我效能与脑卒中防治知识,预防脑率中的发生或复发.