慢性牙周炎患者龈沟液中MCP-1的检测及临床意义

目的:检测慢性牙周炎患者龈沟液中单核细胞趋化蛋白-1(MCP-1)的浓度,并探讨其与牙周临床指标及碱性磷酸酶分泌的关系。方法:采用常规滤纸条法收集慢性牙周炎患者基础治疗前(T1)、基础治疗后(T2)及正常对照组(C)各位点的龈沟液样本,用ELISA法检测各样本中MCP-1及碱性磷酸酶的浓度。采用SPSS11.0软件包对数据进行t检验和直线相关分析。结果:3组受检者中,慢性牙周炎患者龈沟液中MCP-1平均浓度显著高于正常对照组(P<0.01),基础牙周治疗可使牙周炎患者龈沟液中MCP-1含量显著下降(P<0.01),治疗前后MCP-1的浓度变化与菌斑指数、探诊深度、牙龈指数、临床附着丧失以及碱性磷酸酶的分泌均呈正相关。结论:MCP-1作为一个重要的炎性趋化因子参与了慢性牙周炎的发生、发展过程,龈沟液MCP-1可作为牙周病防治及疗效评估的潜在靶分子。     

牙周炎患者龈沟液中sICAM-1含量的研究

目的 :研究牙周炎患者龈沟液可溶性细胞间粘附分子 (sICAM - 1)的含量。方法 :牙周炎患者 15例 ,正常对照20例。用ELISA法检测牙周炎患者龈沟液sICAM - 1的含量。结果 :正常对照龈沟液sICAM - 1含量为145.014±43.745ng/ml,男女间无显著性差异 (p >0.05)。牙周炎患者龈沟液sICAM - 1的含量为23.716±8.195ng/ml,明显低于正常对照 (p <0.01)。结论 :龈沟液sICAM - 1含量的降低与牙周炎有关。     

慢性牙周炎患者龈沟液中白细胞介素-4的检测和意义

目的检测慢性牙周炎患者牙周基础治疗前后龈沟液中白细胞介素-4(IL-4)的质量浓度,探讨IL-4与牙周炎的关系及其在牙周炎发病机制、病情进展等方面所起的作用。方法用滤纸条浸润法采集成年健康者和牙周炎患者治疗前后的龈沟液样本,用酶联免疫吸附测定检测样本中IL-4的质量浓度。结果慢性牙周炎患者龈沟液中IL-4的质量浓度低于健康对照组(P<0.05)。经牙周基础治疗1个月后,IL-4的质量浓度无明显变化,治疗前后的差异无统计意义(P>0.05);IL-4的质量浓度与探诊深度呈显著负相关,与牙龈指数和附着丧失无明显相关性。结论IL-4缺乏可能会导致牙周病的发生,IL-4可作为早期诊断牙周病和检测易患人群的敏感性指标。     

牙周炎患者治疗前后龈沟液中可溶性细胞间粘附分子-1的变化

了解成人牙周炎患者治疗前后龈沟液中可溶性细胞间粘附分子－１含量的变化。方法：采用常规滤纸条法收集正常成人以及成年人牙周炎患者治疗前后的ＧＣＦ样本,用ＥＬＩＳＡ法检测其ｓＩＣＡＭ－１的含量。结果治疗后ＧＦＣ中ｓＩＣＡＭ－１的水平显著下降。     

牙周炎龈沟液免疫球蛋白含量的测定

用改良刻度毛细管收集龈沟液(GCF)，用单扩法测定了50名健康人(H)和32名成人型牙周炎CAP)龈沟液和血清中的IgG、IgA含量．结果：H组GCF中IgG为1．96g／L，是血清的19％。IgA未能测出．AP组GCF中IgG、IgA分别为7．21g／L和0．83g／L．是血清的71％和61％．AP组GCF中IgG与牙龈指数(GI)有正相关．提示GCF中IgG含量与局部炎症程度有关．本研究首次报道了健康人GCF中IgG含量．为研究GCF中Ig含量提供了一个正常值参数。     

牙周炎患者龈沟液中IL—8的含量测定

目的：研究IL－8在牙周炎病程中的变化及与临床指标的关系,方法：采用双抗夹心ABC－ELISA法测定慢性牙周炎（CP）患者,健康对照者以及CP治疗前后患者的龈沟液中IL－8含量,IL－8总量,同时检测临床指标并作相关性检验。结果：CP患者龈沟液中IL－8检出率显著高于健康对照者（P＜0．025）,在CP患者和健康者之间以及CP治疗前后患者的龈沟液中IL－8总量,龈沟液（GCF）量均存在统计学差异（分别为P＜0．01,P＜0．05）,而IL－8含量无统计学差异（P＞0．05）,IL－8总量,GCF量与临床指标存在正相关性（P＜0．01）,结论：IL－8总量在牙周炎病程中呈动态性改变,检测GCF中IL－8的水平对评价牙周炎的程度及指导临床治疗有一定价值。     

慢性牙周炎龈沟液中硫离子水平与临床相关性研究

目的:分析慢性牙周炎(CP)患者龈沟液中硫离子(su lfides)水平的变化与临床牙周指数的相关关系及其对诊断预后的意义。方法:采用金刚牙周诊断仪进行龈沟液硫离子和牙周临床指标测定。选定实验组(T):36例慢性牙周炎患者,57颗牙位,共342个位点。其中健康牙位(T1)21颗,位点126个;炎症牙位(T2)36颗,位点216。对照组(C):全身及牙周健康者8例,16颗牙位,共96个位点。测定所选位点龈沟液(GCF)中硫化物水平(su lcussu lph ide level,SUL),牙周袋探诊深度(prob ing depth,PD),牙周临床附着丧失水平(c lin ical attachm ent level,CAL),龈沟出血指数(su lcus b leed ing index,SB I)。所有统计结果均采用SPSS11.0进行统计学分析。结果:1)牙周健康对照组(C)GCF中硫离子SUL的浓度均值为(0.0648±0.0169)pg/mL,明显低于慢性牙周炎炎症牙位组(T2)(0.3249±0.0489)pg/mL及慢性牙周炎健康牙位组(T1)(0.1160±0.0271)pg/mL;慢性牙周炎炎症牙位组(T2)GCF中硫离子(SUL)的浓度均值均高于正常对照组及慢性牙周炎健康牙位组(T1)。2)经相关性分析,慢性牙周炎炎症牙位组(T1)GCF中SUL的浓度均值与PD、SB I和CAL均呈正相关关系。而慢性牙周炎健康牙位组(T1)及正常对照组(C)GCF中SUL的浓度均值与PD、SB I及CAL间无相关性。结论:慢性牙周炎(CP)炎症牙位组龈沟液中硫离子(SUL)的浓度均值与牙周临床指标之间具有相关关系,其水平的高低变化可客观反映牙周组织的炎症状态。     

牙周炎患者龈沟液中乳酸脱氢酶水平分析

目的：探讨龈沟液中乳酸脱氢酶（ＬＤＨ）水平对于慢性成人牙周炎患者诊断及预后观察的意义。方法：酶动力学方法。结果：患病部位和健康部位龈沟液中ＬＤＨ水平有非常显著差异（Ｐ〈０．００１）。探诊深度和龈沟液中ＬＤＨ水平呈正相关（Ｐ〈０．０５）。附着丧失水平和龈沟液中ＬＤＨ水平呈正相关（Ｐ〈０．０５）。结论：龈沟液中ＬＤＨ水平对于慢性成人牙周炎的诊断和疗效监测具有一定的临床意义。     

牙周炎大鼠龈沟液中β防御素浓度的检测

目的：通过建立大鼠的牙周炎模型,检测β防御素在牙周健康大鼠和慢性牙周炎大鼠龈沟液中浓度的变化,探讨β防御素与牙周炎的关系.方法：结扎法建立慢性牙周炎大鼠（20只）模型,分别采集结扎前,结扎后第2,4,6周末4组龈沟液（gingival crevicular fluid,GCF）样本,首先用ELISA方法检测β防御素总蛋白的浓度,然后应用蛋白质印迹法分别检测β防御素1,2的蛋白表达,并傲半定量分析.利用SPSS 16.0统计软件进行单因素方差分析,P＜0.05为差异有统计学意义.结果：临床观察慢性牙周炎大鼠模型的形成过程,在第2周末牙龈炎形成期龈沟液β防御素蛋白与β-防御素2浓度与结扎前均明显上升（P＜0.05）;在第4,6周末牙周炎期,两者的表达强度又明显降低（P＜0.01）;β-防御素1浓度随时间未见明显改变（P＞0.05）.结论：β防御素总蛋白与β-防御素2浓度在牙周炎大鼠的龈沟液（GCF）中两者的浓度显著上升后又降低,提示两者可与慢性牙周炎的病程和致病机制密切相关.     

牙周炎患者龈沟液中的碱性磷酸酶水平

选择慢性期牙周炎患者２０人５５个牙，健康者５人１０个牙，用滤纸条采集龈沟液并以全自动生化分析仪测出碱性磷酸酶水平。同时记录牙龈指数、探诊深度和附着水平。结果表明ＡＬＰ活性水平与临床数呈正相关，提示牙周炎及齿沟中ＡＬＰ水平可能反映牙周组织的破坏程度。     

牙周炎患者龈沟液中IL—1活性检测

目的：通过对牙周炎龈沟液（ｇｉｎｇｉｖａｌｃｒｅｖｉｃｕｌａｒｆｌｕｉｄ，ＧＣＦ）中ＩＬ－１活性进行检测，初步探讨ＩＬ－１在牙周炎症中的作用。方法：以成人牙周炎为研究对象，通过细胞检测法对患牙ＧＣＦ中ＩＬ－１活性进行检测。结果：在患牙ＧＣＦ中可测到具有较高活性的ＩＬ－１，而在健康ＧＣＦ中未能测到。结论：牙周炎时局部有活性ＩＬ－１渗出，ＩＬ－１是参与牙周炎症反应的重要细胞因子。     

Interleukin-1 and IL-1 receptor antagonist in gingival crevicular fluid

BACKGROUND/AIMS: This study aimed to investigate the cytokine IL-1beta and its receptor antagonist IL-1ra in gingival crevicular fluid (GCF), in patients with adult periodontitis. METHOD: A total of 40 GCF samples were harvested from 10 subjects with moderate to severe adult periodontitis and 10 healthy controls. Subjects were selected from both genders, with all the upper anterior teeth present, and with no relevant systemic illness, pregnancy or recent medication. All subjects were non-smokers and had not received any periodontal therapy within the preceding 3 months. Deep bleeding sites, deep non-bleeding sites and healthy sites were investigated in relation to upper anterior teeth. Clinical measurements were recorded for each site, after obtaining a GCF sample. IL-1beta and IL-1ra were quantified using new commercially available ELISA kits (Quantikine), and could be detected in all samples. RESULTS: The mean concentration for IL-1beta was 0.11 (SD 0.14) pg/microl for bleeding periodontitis sites, 0.04 (0.05) pg/microl for non-bleeding periodontitis sites, and 0.01 (0.03) pg/microl for healthy sites (p<0.001). In contrast, the mean concentration for IL-1ra was 6.99 (9.78) pg/microl for healthy sites, 0.59 (0.44) pg/microl for non-bleeding periodontitis sites, and 0.44 (0.36) pg/microl for bleeding periodontitis sites (p<0.001, except for comparisons between bleeding and non-bleeding periodontitis sites, p>0.05). For healthy sites, a strong inverse relationship was found between IL-1beta and IL-1ra levels in GCE. CONCLUSIONS: The results suggest a strong relationship between the severity of adult periodontitis and the increasing GCF levels of IL-1beta and decreasing levels of IL-1ra.     

Modulation of KGF-1 gene expression in oral fibroblasts by ripe areca nut extract

BACKGROUND: Areca chewing is a common habit of Asians, leading to a high propensity for a variety of oral diseases in this population. This research aimed to study the expression level of genes in oral fibroblast cell lines in response to exposure to ripe areca nut extract (rANE). METHODS: Fifteen oral fibroblast cell lines obtained from individuals aged 20-77 years were established. Treatment of a cell line with 40 micro g/ml rANE for 24 h was performed to achieve RNA for cDNA microarray analysis. RESULTS: Among some 320 genes exhibiting detectable expression levels, 14 were up-regulated and 26 were down-regulated more than 2.5-fold. Semi-quantitative RT-PCR analysis suggested that up-regulation of IL-6 expression and down-regulation of PDGFR, APP-1 and KGF-1 expressions in multiple cell lines assayed, were compatible with the results of the microarray analysis. Using quantitative real-time RT-PCR analysis, a remarkable down-regulation of KGF-1 expression in response to 40 microg/ml rANE, ranging 1.5-ninefold as compared to controls, was found in 60% (9/15) of the cell lines. CONCLUSION: This study established a novel toxicogenomic database for rANE. The down-regulation of KGF-1 expression in oral fibroblast cell lines potentially impairs the proliferation of overlying keratinocytes, which could partially explain the frequent epithelial atrophy observed in chronic areca chewers in vivo.     

正畸治疗中牙龈蛋白K与牙龈炎性反应的关系

目的分析牙龈蛋白K（gingipain K,Kgp）在正畸治疗中对牙龈炎性反应的影响。方法选择青少年牙龈健康者45例,分别取矫治器戴入前与矫治器戴入后3个月的龈沟液,应用16S rDNA PCR技术检测各样本中的牙龈卟啉单胞菌（P.g）及Kgp,采用SPSS17.0软件包对数据进行统计学分析。结果矫治器戴入前Kgp检出率为35.71%,矫治器戴入后Kgp检出率为67.86%,两者之间差异显著（P<0.05）。结论正畸治疗患者在矫治器戴入后Kgp检出率增加,出现牙龈炎性反应。     

Changes in transforming growth factor-beta1 in gingival crevicular fluid following periodontal surgery

OBJECTIVES: Growth factors play a major part in wound healing, including in the periodontium. However, the presence of growth factors in gingival crevicular fluid (GCF) in humans during periodontal wound healing has not yet been determined. Our hypothesis is that such factors are present in GCF and that changes in their levels might be of value as a prognostic marker of wound-healing activity and therapeutic progress following periodontal surgery. The aim of this study was therefore to measure transforming growth factor-beta1 (TGF-beta1) in GCF collected from sites that have undergone guided tissue regeneration (GTR) and conventional flap (CF) surgery and to compare these with GCF collected from unaffected healthy sites. MATERIALS AND METHODS: GCF samples were collected, using filter paper strips, at baseline (pre-surgical) and then at intervals up to 26 weeks from 16 patients undergoing GTR and from 11 patients undergoing CF surgery. After elution and acid treatment, TGF-beta1 levels were measured by ELISA. RESULTS: Treatment of periodontal defect sites significantly reduced the mean probing pocket depth (PPD) and improved the mean lifetime cumulative attachment loss (LCAL). Average GCF volumes also significantly increased at all sites at 2 weeks post-surgery and thereafter declined to baseline levels, except at the GTR test sites that were still elevated at 7 weeks. TGF-beta1 could be detected in almost all GCF samples, and 2 weeks after surgery, the average levels increased two-fold at the surgically treated but not at the control sites, which remained unchanged. CONCLUSION: TGF-beta1 is readily detectable in GCF and increases transiently following periodontal surgery. This suggests that changes in the levels of this growth factor in GCF might be useful for monitoring the progress of periodontal repair and regeneration.     

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目的探讨具核梭杆菌(Fusobacterium nucleatum,Fn)在正畸牙龈炎性反应发生发展中的作用。方法收集2011年8月至2012年2月在济南市口腔医院正畸科接受固定矫治并伴有不同程度牙龈炎性反应的牙颌畸形患者63例组成正畸治疗组;正畸治疗前未戴用矫治器的牙周健康者30例组成正常对照组;牙周科就诊的慢性牙周炎患者35例组成牙周炎组。记录患者牙龈指数(GI),分别采集牙周袋最深处或龈沟液标本,采用16SrDNAPCR技术对Fn进行检测,分析Fn的检出率与GI之间的关系。结果 128例患者临床标本中,共扩增出Fn87例,检出率67.97%。其中正畸治疗组检出率为69.84%(44/63);正常对照组检出率为46.67%(14/30);牙周炎组检出率为82.86%(29/35)。正畸治疗组的Fn检出率明显高于正常对照组,牙周炎组高于正畸治疗组和正常对照组,三者之间差异有统计学意义(χ2=9.843,P<0.05)。Spearman等级相关分析显示,Fn的检出率与牙龈指数之间存在明显的正相关关系(rs=1.000,P<0.01)。结论 Fn与正畸治疗中牙龈炎性反应的发生发展密切相关,正畸医生应高度重视正畸治疗前与治疗过程中的口腔卫生指导和监督。     

Daytime variations of interleukin-1beta in gingival crevicular fluid

Interleukin-1 β (IL-1 β ) is an important parameter in periodontal research because of its role in inflammation and bone resorption. One measure used to assess local IL-1 β concentrations is analysis of its levels in gingival crevicular fluid (GCF). While studies on serum IL-1 β concentrations indicate a circadian rhythm of this parameter, nothing is known about daytime variations of IL-1 β in GCF. The present study thus aimed to analyse such variations. Daytime variations of GCF-IL-1 β between 08:00 and 22:00 h were assessed, with a time resolution of 2 h, in 28 periodontally healthy subjects.The data showed a significant variation throughout the day, with the lowest concentrations and total amounts in the morning and the highest in the evening. The effect sizes of comparisons between morning and evening samples were medium to high and corresponded in magnitude to those reported in other published research comparing healthy sites and those affected by periodontitis. The smallest daytime variations were found to occur between 12:00 h and 18:00 h. It is concluded that daytime variations in GCF-IL-1 β are large enough to be able to mimic or mask differences caused by clinical factors.