Canal and Isthmus Debridement Efficacy of the VPro EndoSafe Negative-pressure Irrigation Technique

Introduction

This study compared canal and isthmus debridement efficacies between side-vented needle irrigation (SNI) and the VPro EndoSafe (VPro) negative-pressure device (Vista Dental, Racine, WI) in mesial roots of mandibular first molars with narrow isthmuses in a closed-canal design.

Methods

Twenty teeth with narrow isthmuses were selected using micro–computed tomography scanning. Collagen solution was reconstituted with 1% NH₄OH to simulate debris and injected into canals via vacuum. Canals were instrumented to size 40/.04. Final irrigation was performed with 17% EDTA and 6% NaOCl using SNI with a 30-G needle or the VPro system. Roots were demineralized, and Masson trichrome–stained sections were prepared at 1.2 to 3.2 mm from the anatomic apex for a total of 6 canal levels. Debris-filled areas within the canals and isthmuses were measured using ImageJ software (National Institutes of Health, Bethesda, MD) and statistically analyzed using repeated-measures analysis.

Results

There was no significant difference between techniques and debridement efficacy among the 6 levels within the canal (P = .108). The debridement efficacy at 1.6 mm differed significantly from that at 3.2 mm. VPro had significantly more debris at isthmus levels 2.0 and 2.4 mm than SNI (P = .009). A significant difference was observed between the canal and the isthmus (P < .001). Neither VPro nor SNI removed debris completely from the apical third or isthmus.

Conclusions

VPro EndoSafe did not differ significantly from SNI in the overall debridement efficacy in the apical third of mesial roots of mandibular first molars.     

Smear Layer Removal and Canal Cleanliness Using Different Irrigation Systems (EndoActivator,EndoVac, and Passive Ultrasonic Irrigation): Field Emission Scanning Electron Microscopic Evaluation in an In Vitro Study

IntroductionThe purpose of this study was to evaluate the effectiveness of different irrigating methods in removing the smear layer at 1, 3, 5, and 8 mm from the apex of endodontic canals.MethodsSixty-five extracted single-rooted human mandibular premolars were decoronated to a standardized length of 16 mm. Specimens were shaped to ProTaper F4 (Dentsply Maillefer, Ballaigues, Switzerland) and irrigated with 5.25% NaOCl at 37°C. Teeth were divided into 5 groups (2 control groups [n = 10] and 3 test groups [n = 15]) according to the final irrigant activation/delivering technique (ie, sonic irrigation, passive ultrasonic irrigation [PUI], or apical negative pressure). Root canals were then split longitudinally and observed by field emission scanning electron microscopy. The presence of debris and a smear layer at 1, 3, 5, and 8 mm from the apex was evaluated. Scores were analyzed by Kruskal-Wallis and Mann-Whitney U tests.ResultsThe EndoActivator System (Dentsply Tulsa Dental Specialties, Tulsa, OK) was significantly more efficient than PUI and the control groups in removing the smear layer at 3, 5, and 8 mm from the apex. The EndoVac System (Discus Dental, Culver City, CA) removed statistically significantly more smear layer than all groups at 1, 3, 5, and 8 mm from the apex. At 5 and 8 mm from the apex, PUI and the EndoVac did not differ statistically significantly, but both performed statistically better than the control groups.ConclusionsIn our study, none of the activation/delivery systems completely removed the smear layer from the endodontic dentine walls; nevertheless, the EndoActivator and EndoVac showed the best results at 3, 5, and 8 mm (EndoActivator) and 1, 3, 5, and 8 mm (EndoVac) from the apex.     

Comparison of Sealer Penetration Using the EndoVac Irrigation System and Conventional Needle Root Canal Irrigation

Introduction

The aim of this study was to compare the effect of the EndoVac irrigation system (SybronEndo, Orange, CA) and conventional endodontic needle irrigation on sealer penetration into dentinal tubules.

Methods

Forty single-rooted, recently extracted human maxillary central incisors were randomly divided into 2 groups according to the irrigation technique used: conventional endodontic needle irrigation and EndoVac irrigation. All teeth were instrumented using the ProFile rotary system (Dentsply Maillefer, Ballaigues, Switzerland) and obturated with gutta-percha and AH Plus sealer (Dentsply DeTrey, Konstanz, Germany) labeled with fluorescent dye. Transverse sections at 1, 3, and 5 mm from the root apex were examined using confocal laser scanning microscopy. The total percentage and maximum depth of sealer penetration were then measured.

Results

Mann-Whitney test results showed that EndoVac irrigation resulted in a significantly higher percentage of sealer penetration than conventional irrigation at both the 1- and 3-mm levels (P < .05). However, no difference was found at the 5-mm level. The 5-mm sections in each group showed a significantly higher percentage and maximum depth of sealer penetration than did the 1- and 3-mm sections (P < .05).

Conclusions

The EndoVac irrigation system significantly improved the sealer penetration at the 1- to 3-mm level over that of conventional endodontic needle irrigation.     

Comparison of debris removal with three different irrigation techniques

Introduction

The purpose of this study was to compare the effectiveness of debris removal between EndoVac, PiezoFlow, or needle irrigation (Max-i-Probe) in mandibular molars.

Methods

The mesial roots of 30 extracted mandibular molars were mounted in resin by using the K-Kube and then sectioned at 2 and 4 mm from the apex. The specimens were reassembled and instrumented to size 40/0.04. A standard irrigation protocol was used during instrumentation in the PiezoFlow and Max-i-Probe groups. During instrumentation of teeth in the EndoVac group, the manufacturer’s recommendations for irrigation were followed. Images of the canals and isthmuses were taken before final irrigation. The percentage of cleanliness for each canal and isthmus was calculated by using interactive software. The specimens were reassembled for final irrigation with EndoVac, PiezoFlow, and Max-i-Probe by using similar volumes of irrigants. Images were again used to calculate cleanliness. Comparisons of canal and isthmus cleanliness before and after final irrigation were made by using paired t tests, and the groups were compared with repeated-measures analysis of variance (P < .05).

Results

There were no statistically significant differences in canal and isthmus cleanliness among all 3 groups at 2 and 4 mm from working length before or after final irrigation. Canal and isthmus cleanliness significantly improved at all levels after the final irrigation regimen in each group.

Conclusions

Final irrigation by using EndoVac, the PiezoFlow, or Max-i-Probe with similar volumes of irrigants significantly improved canal and isthmus cleanliness.     

Effect of Different Final Irrigation Methods on the Removal of Calcium Hydroxide from an Artificial Standardized Groove in the Apical Third of Root Canals

Introduction

The aim of this study was to compare the efficacy of conventional syringe, ultrasonic, EndoVac (Discus Dental, Culver City, CA), and Self-Adjusting File (SAF) (Re-Dent-Nova, Ra'nana, Israel) irrigation systems in removing calcium hydroxide (Ca[OH]₂) from simulated root canal irregularities.

Methods

The root canals of 88 extracted single-rooted teeth were prepared using ProTaper rotary instruments (Dentsply Maillefer, Ballagiues, Switzerland) up to size F4. The roots were split longitudinally, and a standardized groove was prepared in the apical part of 1 segment. The root halves were reassembled, and Ca(OH)₂ medicament was placed into the root canals using a Lentulo spiral. The roots were randomly divided into 4 experimental groups and 2 control groups according to the different irrigation systems used: conventional syringe irrigation, continuous passive ultrasonic irrigation (PUI), EndoVac irrigation, and SAF irrigation. Each group was then divided into 2 subgroups (n = 10) according to the irrigation protocol: subgroup 1: 10 mL 2.5% NaOCl and subgroup 2: 10 mL 17% EDTA + 10 mL 2.5% NaOCl. The amount of remaining medicament was evaluated under a stereomicroscope at 30× magnification using a 4-grade scoring system. The influences of the different Ca(OH)₂ medicament removal methods and irrigation protocols were statistically evaluated using 2-way analysis of variance and Tukey post hoc tests.

Results

In the NaOCl-irrigated groups, PUI removed significantly more Ca(OH)₂ medicament than the other techniques (P < .05). There was no significant difference among the other groups (P > .05). In the EDTA/NaOCl-irrigated groups, the SAF and PUI removed significantly more Ca(OH)₂ than the other techniques (P < .05).

Conclusions

The use of the SAF system with the combination of EDTA and NaOCl enhanced Ca(OH)₂ removal when compared with the use of only NaOCl irrigation with the SAF. Continuous PUI and SAF were more effective than EndoVac, and conventional syringe irrigation in the removal of the Ca(OH)₂ medicament from an artificial standardized groove in the apical part of the root canal.     

Assessment of Irrigant Agitation Devices in Simulated Closed and Open Root Canal Systems

IntroductionThe in vitro efficacy of irrigant activation devices has not been contrasted to their safety. This was attempted in this study using apically closed versus patent simulated root canal systems in epoxy resin models, with the latter featuring a simulated periapical lesion.MethodsAll 72 models had 2 joining canals connected by an isthmus, which was filled with dentin debris. The simulated periapical lesion was filled with colored gelatin in the 36 respective models. Canals were irrigated with 1.3% sodium hypochlorite. Samples were divided into 4 subgroups per system (n = 9): conventional irrigation, sonic low (EndoActivator; Dentsply Sirona, Charlotte, NC) and high frequency (EDDY; VDW, Munich, Germany), and ultrasonic agitation of the irrigant (always applying 3 cycles of 20 seconds). The total cleared surface areas (mm²) in the simulated isthmus and periapical lesion were compared between systems and devices using parametric tests (P < .05).ResultsThe cleaning of the isthmus was more effective in the apically open compared with the closed systems and was also significantly influenced by the agitation method (P < .001). In the closed systems, EDDY and ultrasonic agitation achieved the significantly (P < .05) best cleaning of the isthmus. In the open systems, ultrasonic agitation showed the single best result (P < .05). EDDY caused by far the highest and ultrasonic agitation the lowest dissolution of the gelatin in the simulated periapical lesion.ConclusionsUnder the conditions of this study, ultrasonic agitation of a previously administered sodium hypochlorite irrigant was more laterally targeted and thus safer and more effective than sonic agitation methods.     

Comparative Antibacterial Efficacies of Hydrodynamic and Ultrasonic Irrigation Systems In Vitro

Introduction

To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. The in vitro bactericidal effects of a hydrodynamic system and a passive ultrasonic irrigation system were compared.

Methods

Single-rooted extracted teeth (n = 250) were contaminated with suspensions of Enterococcus faecalis ATCC 29212, mixed aerobic cultures, or mixed anaerobic cultures. First, the antibacterial effects of the hydrodynamic system (RinsEndo), a passive ultrasonic irrigation system (Piezo smart), and manual rinsing with 0.9% NaCl (the control) were compared. Colony-forming units were counted. Second, the 2 systems were used with 1.5% sodium hypochlorite (NaOCl) alone or NaOCl + 0.2% chlorhexidine (CHX). The colony-forming units in the treated and untreated roots were determined during a period of 5 days.

Results

Both irrigation systems reduced bacterial numbers more effectively than manual rinsing (P < .001). With NaCl, ultrasonic activated irrigation reduced bacterial counts significantly better than hydrodynamic irrigation (P = .042). The NaOCl + CHX combination was more effective than NaOCl alone for both systems (P < .001), but hydrodynamic irrigation was more effective with NaOCl + CHX than the passive ultrasonic irrigation system.

Conclusions

Both irrigation systems, when combined with NaOCl + CHX, removed bacteria from root canals.     

Efficacy of different final irrigation activation techniques on smear layer removal

Introduction

Different techniques and irrigant delivery devices have been proposed to increase the flow and distribution of irrigating solutions within the root canal system. The aim of this study was to compare smear layer removal after final irrigant activation with apical negative pressure (ANP), manual dynamic agitation (MDA), and passive ultrasonic irrigation (PUI).

Methods

Forty single-rooted human mandibular premolars were decoronated to a standardized length of 16 mm. They were cleaned and shaped by using ProTaper system to size F4 and NaOCl 2.5%. The specimens were divided into 4 equal groups (n = 10) according to the final irrigation activation technique: group 1, passive irrigation (PI); group 2, apical negative pressure (ANP) (EndoVac); group 3, manual dynamic activation (MDA); and group 4, passive ultrasonic irrigation (PUI). Samples were split longitudinally and examined under scanning electron microscope for smear layer presence.

Results

PI and PUI had the highest smear scores, with no significant differences between them. This was followed by MDA and finally ANP, which showed the statistically significant lowest mean score at P ≤ .05.

Conclusions

Final irrigant activation with ANP and MDA resulted in better removal of the smear layer than with PUI or PI.     

Comparative Analysis of Dentinal Erosion after Passive Ultrasonic Irrigation versus Irrigation with Reciprocating Activation: An Environmental Scanning Electron Study

Introduction

The aim of this study was to assess ex vivo the erosive effects of passive ultrasonic irrigation versus irrigation with reciprocating activation on the dentinal surface of the root canal at 3 predetermined levels using environmental scanning electron microscopy.

Debris Removal from the Mesial Root Canal System of Mandibular Molars with Laser-activated Irrigation

Introduction

The purpose of this study was to compare in vitro the canal and isthmus debridement of manual-dynamic, passive ultrasonic, and laser-activated irrigation with an Er:YAG laser in mesial roots of human mandibular molars.

Efficacy of 3 Supplementary Irrigation Protocols in the Removal of Hard Tissue Debris from the Mesial Root Canal System of Mandibular Molars

IntroductionInstrumentation of the mesial root canal system of mandibular molars may hinder disinfection by packing hard tissue debris within the isthmuses. The removal of accumulated hard tissue debris (AHTD) by 3 supplemental irrigation systems, 2 ultrasonically activated and 1 multisonic, was assessed with micro–computed tomographic imaging.MethodsTwenty-four extracted mandibular molars with 2 mesial canals connected by an isthmus and converging to a single foramen were selected. After preparation of the mesial canals with WaveOne Gold instruments (Dentsply Maillefer, Ballaigues, Switzerland), anatomically matched specimens were assigned to 3 final irrigation protocols (n = 8): intermittent ultrasonic (IU) with an ultrasonically energized 200-μm wire (Irrisafe; Satelec, Bordeaux, France), continuous ultrasonic (CU) with an ultrasonic irrigation needle (ProUltra PiezoFlow, Dentsply Maillefer), and GentleWave (GW) system (Sonendo Inc, Laguna Hills, CA). Specimens were scanned (SkyScan 1176; Bruker-microCT, Kontich, Belgium) at 17.18-μm pixel size before and after preparation and irrigation protocols. Data sets were coregistered, and the percentage reduction of AHTD calculated within the canals and isthmus for each specimen was statistically compared using 1-way analysis of variance and post hoc Tukey tests with a 5% significance level.ResultsThe mean percentage reduction of AHTD in canals and isthmuses was significantly higher for GW (96.4% and 97.9%, respectively) than for CU (80.0% and 88.9%, respectively) (P < .05). AHTD reduction for IU (91.2% and 93.5%, respectively) did not differ significantly from GW and CU (P > .05).ConclusionsGW achieved greater efficacy in the removal of AHTD from the mesial root canal system of mandibular molars compared with CU but not IU. The efficacy of CU and IU was comparable.     

Comparative Evaluation of Calcium Hypochlorite and Sodium Hypochlorite Associated with Passive Ultrasonic Irrigation on Antimicrobial Activity of a Root Canal System Infected with Enterococcus faecalis: An In Vitro Study

Introduction

The purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (Ca[OCl]₂) and sodium hypochlorite (NaOCl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with Enterococcus faecalis.

Methods

The root canals of 60 single-rooted bovine extracted teeth were enlarged up to a file 45, autoclaved, inoculated with Enterococcus faecalis, and incubated for 30 days. The samples were divided into 6 groups (n = 10) according to the protocol for decontamination: G1: no treatment; G2: distilled water; G3: 2.5% NaOCl; G4: 2.5% Ca(OCl)₂; G5: 2.5% NaOCl with ultrasonic activation; and G6: 2.5% Ca(OCl)₂ with ultrasonic activation (US). Microbiological testing (colony-forming unit [CFU] counting) was performed to evaluate and show, respectively, the effectiveness of the proposed treatments. Data were subjected to 1-way analysis of variance followed by the post hoc Tukey test (α = 0.05).

Results

Groups 1 and 2 showed the highest mean contamination (3.26 log₁₀ CFU/mL and 2.69 log₁₀ CFU/mL, respectively), which was statistically different from all other groups (P < .05). Group 6 (Ca[OCl]₂ + US) showed the lowest mean contamination (1.00 log₁₀ CFU/mL), with no statistically significant difference found in groups 3 (NaOCl), 4 (Ca[OCl]₂), and 5 (NaOCl + US) (P < .05).

Conclusions

Ca(OCl)₂ as well as passive ultrasonic irrigation can aid in chemomechanical preparation, contributing in a significant way to the reduction of microbial content during root canal treatment.     

Effectiveness of Final Irrigant Protocols for Debris Removal from Simulated Canal Irregularities

Introduction

The aim of this in vitro study was to compare the effectiveness of saline, 2.5% sodium hypochlorite, and 2% chlorhexidine, with or without passive ultrasonic irrigation (PUI), in debris removal from simulated canal irregularities within prepared root canals.

Methods

Ninety bovine lateral incisors were randomly divided into 3 main groups (n = 30) based on the irrigant and prepared with hand files attached to an oscillating handpiece (NSK, Tokyo, Japan) up to a size #80 K-file. Next, the teeth were split longitudinally, and a standardized groove was prepared into the apical third and filled with dentin debris. After the halves were reassembled, they were placed in a muffle. Each main group was randomly subdivided into 2 groups (n = 14) and was treated with different final irrigation protocols. In the sodium hypochlorite/PUI, chlorhexidine/PUI, and saline/PUI groups, the solution was ultrasonically activated 3 times for 20 seconds. In the remaining groups, PUI was not performed. Specimens were scored for debris removal and analyzed under a scanning electron microscope.

Results

An association was observed between the score of debris removal and protocols using PUI (P < .05). No association was observed between the scores of debris removal and the irrigants (P = .87).

Conclusions

Final irrigation protocols that used PUI were more effective in removing debris from simulated canal irregularities into the apical third than those that did not use it.     

Removal of Root Canal Fillings in Curved Canals Using Either Mani GPR or HyFlex NT Followed by Passive Ultrasonic Irrigation

This study compared the efficacy of HyFlex NT (HF; Coltene, New York, NY) and Mani GPR (MN; Mani Inc, Tokyo, Japan) systems followed by a supplementary approach with passive ultrasonic irrigation (PUI) in removing the filling material from curved root canals during retreatment. The mesial canals from 20 extracted mandibular molars with Vertucci type IV configuration were instrumented and obturated (N = 40). Each mesial canal was retreated using either HF or MN instruments, alternating the system used per canal from root to root. The final preparation size was 40/0.04 for both systems. The volume of canal filling material was assessed by means of micro–computed tomographic (micro-CT) imaging before and after retreatment, and the working time was recorded. Canals still showing remnants of filling material were subjected to a supplementary approach with PUI, and another micro-CT scan was taken. The percentage of filling material removed with MN instruments and HF was similar (95.5% and 92.7%, respectively) (P > .05). MN required significantly less time to remove the filling material (P < .05). The supplementary approach with PUI significantly enhanced the removal of filling material (P < .05). Both systems were equally effective in removing the root canal filling during retreatment. MN was faster than HF. The supplementary approach with PUI significantly improved filling material removal.     

Apical extrusion of sodium hypochlorite using different root canal irrigation systems

Introduction

Root canal irrigation carries a risk of extrusion of irrigant into the periapical tissues. The objective of this study was to compare different irrigation systems in matched pairs of teeth prepared to an apical size of 35.06 and 50.06 by measuring the frequency and extent of apical extrusion of sodium hypochlorite (NaOCl) into a simulated periapical environment. The null hypothesis was tested that there is no difference between systems.

Methods

Bilaterally matched pairs (n = 10) of single-canal extracted human anterior teeth were instrumented to an apical size of either 35.06 or 50.06. Teeth were embedded in a gel containing the pH-sensitive dye M-cresol purple that changes from yellow at pH 7.4 to purple at pH 9. Root canals were irrigated with 6% NaOCl (pH 11) by using EndoActivator (EA), EndoVac (EV), Rispi-Sonic/MicroMega 1500 (MM), passive ultrasonic irrigation (PUI), and syringe irrigation with a slot-tipped needle (SN), so that each tooth underwent all irrigation procedures in a randomized crossover design. Apical extrusion was evaluated by image analyses.

Results

The frequency of extrusion was less in teeth with apical preparation size 35.06 (36%) compared with 50.06 (60%) (P = .014) and was dependent on the irrigation system in 35.06 (P = .039) but not 50.06 groups. In the 35.06 group the frequency of extrusion was less for EV than for MM and SN (both P = .029). The extent of extrusion was less for MM compared with PUI (P = .024) and SN (P = .046) in the 35.06 group and greater for SN compared with all other systems in the 50.06 group (P < .05). The null hypothesis was rejected.

Conclusions

The frequency of apical extrusion of NaOCl was dependent on the type of root canal irrigation system and apical preparation size. The extent of extrusion depended on the irrigation system, with syringe and slotted-needle irrigation resulting in the greatest extent of extrusion.     

Efficacy and Safety of Various Active Irrigation Devices When Used with Either Positive or Negative Pressure: An In Vitro Study

Introduction

The purpose of this in vitro study was to evaluate and compare the efficacy and safety of different devices available for canal cleansing.

Methods

The following systems were tested: passive ultrasonic irrigation, EndoVac (Discus Dental, Culver City, CA), and the irrigation ultrasonic needle (ProUltra PiezoFlow Irrigation Ultrasonic Needle; Dentsply Tulsa Dental Specialties, Tulsa, OK) used in both the injection mode (IUNI) and the aspiration mode (IUNA). In the control group, traditional irrigation with a syringe and side-vented needle was used. A resin model was used with 4 lateral canals (respectively at 2, 5, 8, and 11 mm from the apical foramen) filled with bovine pulp stained with fuchsin. The model also included a 2-mm chamber in communication with the apex, again filled with bovine pulp, which enabled the measurement of the extrusion of NaOCl beyond the apex.

Results

With regard to efficacy, the most effective systems were found to be those using the ultrasonic needle, either in aspiration or injection modes; EndoVac was the least effective. Conversely, IUNI was found to bring the highest risk with regard to the extrusion of sodium hypochlorite beyond the apex. EndoVac was the safest but only by a slight margin compared with IUNA and passive ultrasonic irrigation.

Conclusions

Based on this study, the system that best reconciles efficacy and safety appears to be IUNA.     

Efficacy of sonic and ultrasonic activation for removal of calcium hydroxide from mesial canals of mandibular molars: a microtomographic study

Background

The purpose of this study was to use micro-computed tomography (micro-CT) scanning to evaluate the efficacy of sonic and passive ultrasonic irrigation (PUI) on calcium hydroxide (Ca[OH]₂) removal and to measure the volume and percentage of Ca(OH)₂ remaining in the root canal system.

Methodology

The root canals of 46 extracted human mandibular molar teeth were prepared with rotary instruments and randomly assigned to two experimental groups (n = 40) as well as positive and negative controls (n = 6). In each experimental group, 20 teeth were assigned to each irrigation protocol, sonic or passive ultrasonic irrigation. All experimental teeth and the positive controls were filled with Ca(OH)₂, whereas the negative control teeth did not receive Ca(OH)₂. All teeth were scanned using micro-CT scanning to determine the dressing volume. After 7 days, the Ca(OH)₂ was removed in the experimental groups using rotary instrumentation only, and the teeth were again scanned using micro-CT scanning to calculate volume and percentage of Ca(OH)₂ removed. Positive control teeth were not subjected to rotary instrumentation. Experimental samples were then irrigated using either sonic or passive ultrasonic and the volume of remaining Ca(OH)₂ was calculated using micro-CT.

Results

Remnants of Ca(OH)₂ were found in all experimental groups. No Ca(OH)₂ was found in the negative controls, whereas a mean of 8.7 mm³ of Ca(OH)₂ was recorded in the positive controls. Rotary plus passive ultrasonic irrigation removed significantly more Ca(OH)₂ (85.7%) than rotary plus sonic irrigation (71.5%) (p < 0.001).

Conclusions

The combination of rotary instrumentation and passive ultrasonic activation for 3 periods of 20 seconds results in significantly lower amounts of Ca(OH)₂ remnants in the canal compared with sonic irrigation.