低钾型周期性瘫痪与红细胞膜ATPase活性及胰岛素水平的相关性

目的 探索低钾型周期性瘫痪(HoPP)与红细胞膜ATPase活性及胰岛素水平的相关性.方法 ①采用磷钼酸比色法测定红细胞膜ATPase活性变化；②应用放免法检测胰岛素水平变化；③应用离子选择电极法检测血K+浓度.结果 Na+ -K+ -ATPase活性及胰岛素水平的改变与HoPP具有相关性.M92+-ATPase和Ca2 -ATPase活性与HOPP无相关性.结论 对HoPP患者进行Na+ -K+ -ATPase活性及胰岛素水平监测,早期给予补钾治疗,可预防HoPP的发病.     

原发性高血压患者红细胞内Ca2+浓度及红细胞膜Ca2+ -Mg2+-ATPase 活性变化与左心室肥厚的关系

目的探讨原发性高血压(EH)患者伴左心室肥厚(LVH)和不伴LVH红细胞内游离Ca2+浓度、红细胞膜Ca2+-Mg2+-ATPase活性的变化及其相互关系.方法检测30名正常血压者(NT组),82例轻度和中度EH患者(其中LVH组40例,非LVH组42例)外周血红细胞内Ca2+浓度及红细胞膜Ca2+-Mg2+-ATPase活性.结果 (1)EH组外周血红细胞内Ca2+浓度显著高于NT组,红细胞膜Ca2+-Mg2+-ATPase活性明显低于NT组.(2)EH患者中LVH组外周血红细胞内Ca2+浓度高于非LVH组,红细胞膜Ca2+ -Mg2+ -ATPase活性低于非LVH组;(3)相关分析表明LVMI与红细胞内Ca2+浓度呈正相关,与红细胞膜Ca2+-Mg2+-ATPase活性呈负相关.结论 EH患者尤其是伴LVH时外周血红细胞内Ca2+浓度升高而红细胞膜Ca2+ -Mg2+ -ATPase活性降低,提示LVH与红细胞内Ca2+过度超负荷有关.     

高血压大鼠动脉平滑肌细胞钙超载与腺苷三磷酸酶

目的探讨自发性高血压大鼠(SHR)动脉血管平滑肌细胞钙超载与腺苷三磷酸酶(ATPase)的关系.方法组织块种植法培养SHR和Wistar-Kyoto( WKY )大鼠胸主动脉平滑肌细胞,应用流式细胞术、生化酶学方法和逆转录聚合酶链反应(RT-PCR)技术等检测SHR和WKY大鼠动脉平滑肌细胞内游离钙浓度([Ca2 ]i)、细胞膜Ca2 -ATPase和Na -K -ATPase活性及其mRNA表达水平.结果SHR动脉平滑肌细胞[Ca2 ]i浓度高于WKY大鼠[(307.7±32.1 vs 118.9±21.4)nmol/L,P<0.01)],Ca2 -ATPase和Na -K -ATPase活性低于WKY大鼠(1.3±0.2 vs 2.6±0.3; 3.1±0.5 vs 4.9±0.5,P均<0.01),细胞质膜Ca2 -ATPase(plasma membrane Ca2 -ATPase, PMCA)亚型1和Na -K -ATPase α1亚单位 mRNA表达低于WKY大鼠(0.231±0.007 vs 0.403±0.021;0.253±0.028 vs 0.634±0.033,P均<0.01).结论SHR动脉平滑肌细胞出现钙超载,其机制可能部分与细胞膜PMCA和Na -K -ATPase活性降低有关,两种ATPase功能降低可能是其基因转录水平下调的结果.     

原发性高血压患者红细胞内Ca^2＋浓度及红细胞膜Ca^2＋—Mg^2＋—ATPase活性变化与左心室肥厚的关系

目的 探讨原发性高血压(EH)患者伴左心室肥厚(LVH)和不伴LVH红细胞内游离Ca2^ 浓度、红细脑膜Ca^2 -Mg^2 -ATPase活性的变化及其相互关系。方法 检测30名正常血压者(NT组)，82例轻度EH患者(其中LVH组40例，非LVH组42例)外周血红细胞内Ca2^ 浓度及红细胞膜Ca^2 -Mg^2 -ATPase活性。结果 (1)EH组外周血红细胞内Ca2^ 浓度显著高于NT组，红细胞膜Ca^2 -Mg^2 -ATPase活性明显低于NT组。(2)EH患者中LVH组外周血红细胞内Ca2^ 浓度高于非LVH组，红细胞膜Ca^2 -Mg^2 -ATPase活性低于非LVH组；(3)相关分析表明LVMI与红细胞内Ca2^ 浓度呈正相关，与红细胞膜Ca^2 -Mg^2 -ATPase活性呈负相关。结论 EH患者尤其是伴LVH时外周血红细胞内Ca2^ 浓度升高而红细胞膜活性降低，提示LVH与红细胞内Ca2^ 过度超负荷有关。     

厄贝沙坦对肾性高血压大鼠血管腺苷三磷酸酶活性和血管紧张素Ⅱ的影响

目的探讨厄贝沙坦对肾性高血压大鼠(RHR)血管Na+-K+-腺苷三磷酸酶(ATPase)、Ca2+-ATPase和血管紧张素Ⅱ(AngⅡ)及血管重塑的影响。方法采用两肾一夹制造肾血管性高血压大鼠模型,18只造模成功的大鼠随机分为RHR组、厄贝沙坦组[50 mg/(kg.d)]、停药组[厄贝沙坦50 mg/(kg.d)灌胃7周后停药1周],每组6只。另设一假手术组(n=6)。测量各组大鼠用药前后血压;8周后,测量大鼠胸主动脉及肠系膜动脉血管壁厚度;采用酶学比色法检测Na+-K+-ATPase、Ca2+-ATPase活性;采用放射免疫技术检测血浆、肠系膜动脉及胸主动脉局部AngⅡ水平。结果与假手术组比较,RHR血压显著升高,血管壁厚度明显增厚,血浆及血管组织AngⅡ明显升高,血管Na+-K+-ATPase、Ca2+-ATPase活性降低;与RHR组比较,厄贝沙坦组大鼠血压明显降低,肠系膜动脉血管壁厚度明显减轻,胸主动脉、肠系膜动脉AngⅡ水平及Ca2+-ATPase活性均升高,Na+-K+-ATPase活性无明显变化;与厄贝沙坦组比较,停药组血压明显升高,胸主动脉及肠系膜动脉Ca2+-ATPase活性明显降低,N...     

高血压大鼠动脉平滑肌细胞钙超载与腺苷三磷酸酶

目的探讨自发性高血压大鼠(SHR)动脉血管平滑肌细胞钙超载与腺苷三磷酸酶(AT-Pase)的关系。方法组织块种植法培养SHR和Wistar-Kyoto(WKY)大鼠胸主动脉平滑肌细胞,应用流式细胞术、生化酶学方法和逆转录聚合酶链反应(RT-PCR)技术等检测SHR和WKY大鼠动脉平滑肌细胞内游离钙浓度([Ca2 ]i)、细胞膜Ca2 -ATPase和Na -K -ATPase活性及其mRNA表达水平。结果SHR动脉平滑肌细胞[Ca2 ]i浓度高于WKY大鼠[(307.7±32.1vs118.9±21.4)nmol/L,P<0.01)],Ca2 -ATPase和Na -K -ATPase活性低于WKY大鼠(1.3±0.2vs2.6±0.3;3.1±0.5vs4.9±0.5,P均<0.01),细胞质膜Ca2 -ATPase(plasmamembraneCa2 -ATPase,PMCA)亚型1和Na -K -ATPaseα1亚单位mRNA表达低于WKY大鼠(0.231±0.007vs0.403±0.021;0.253±0.028vs0.634±0.033,P均<0.01)。结论SHR动脉平滑肌细胞出现钙超载,其机制可能部分与细胞膜PMCA和Na -K -ATPase活性降低有关,两种ATPase功能降低可能是其基因转录水平下调的结果。     

敦煌石室大宝胶囊对衰老大鼠脑组织钙稳态的影响

目的 探讨敦煌石室大宝胶囊对衰老模型大鼠脑组织钙稳态的影响.方法 采用D-半乳糖建立大鼠衰老模型,测定大脑组织内Na+-K+-ATPase、Ca2+-ATPase、Ca2+-Mg2+-ATPase活性和Ca2+含量.结果 模型组大鼠脑组织内Na+-K+-ATPase、Ca2+-ATPase、Ca2+-Mg2+-ATPase活性显著降低,Ca2+含量显著升高(P<0.01,P<0.05);应用敦煌石室大宝胶囊治疗后,治疗组大鼠脑组织内Na+-K+-ATPase、Ca2+-ATPase、Ca2+-Mg2+-ATPase活性增高,Ca2+含量显著降低(P<0.01,P<0.05).结论 敦煌石室大宝胶囊具有调节衰老大鼠脑组织钙稳态的作用.     

高盐饮食对大鼠大脑皮质氧化应激及钠钙泵活性的影响

目的探讨长期高盐饮食对大鼠大脑皮质氧化应激和Na+-K+-ATPase、Ca2+-ATPase活性及其相关基因表达的影响。方法雄性Wistar大鼠随机分为正常盐对照组(NS组,n=13)、8%高盐组(HS组,n=24)和8%高盐+替米沙坦干预组(HS+Tel组,n=12),每2 w测量尾动脉压1次,喂养共24 w。比色法测定大鼠大脑皮质丙二醛(MDA)含量和超氧化物歧化酶(SOD)酶活性;用生化酶学法检测大脑皮质Na+-K+-ATPase、Ca2+-ATPase活性;通过实时荧光定量PCR法检测大脑皮质NAPDH、Na+-K+-ATPaseα1、细胞质膜钙泵(PMCA)1、钠钙交换蛋白(NCX)1和NADPH氧化酶亚单位P22phox mRNA表达。结果与NS组比较,HS组MDA含量增加、SOD活性减弱(P0.05),Na+-K+-ATPase、Ca2+-ATPase活性均降低(P0.05),NADPH氧化酶亚单位P22phox、Na+-K+-ATPaseα1、PMCA1 mRNA表达增加(P0.05),NCX1mRNA表达无明显变化;与HS组比较,HS+Tel组Na+-K+-ATPase、Ca2+-ATPase活性升高(P0.05),其他指标无明显改变。结论高盐饮食可引起大鼠大脑皮质氧化损伤及钠钙泵活性降低。     

丹龙醒脑片对拟血管性痴呆大鼠脑组织AchE、ATP酶活性的影响

目的 :研究丹龙醒脑片(DLXNP)对拟血管性痴呆 (vasculardementia ,VD)模型大鼠脑组织乙酰胆碱酯酶 (AchE)、三磷酸腺苷酶 (AT Pase)活性的影响。方法 :用双侧颈总动脉反复夹闭再灌注同时腹腔注射硝普钠方法建立拟VD大鼠模型 ,取脑组织测AchE、ATPase的活性。结果 :造模后模型组脑组织内Na+ -K+ -ATPase、Ca2 + -Mg2 + -ATPase活性明显降低 (P <0 .0 1) ,AchE活性明显升高 (P <0 .0 1)。与模型组相比 ,小剂量丹龙醒脑片组AchE活性明显降低 (P <0 .0 1) ,Ca2 + -Mg2 + -ATPase活性明显升高 (P <0 .0 5 ) ,丹龙醒脑片大、中剂量组Na+ -K+ -ATPase、Ca2 + -Mg2 + -ATPase活性显著升高(P <0 .0 1～P <0 .0 5 ) ,AchE活性明显降低 (P <0 .0 1)。结论 :丹龙醒脑片能改善脑缺血再灌注后诱导的拟VD大鼠脑组织的能量代谢障碍 ,防止ATPase活性降低 ,并可维持乙酰胆碱 (Ach)的正常水平 ,这些作用可能与其抗血管性痴呆有关。     

地精子皂甙抗缺血再灌性心律失常的作用机制研究

目的研究地精子皂甙对心律失常的作用机制.方法借助缺血-再灌注诱发心律失常大鼠模型,观察地精子皂甙对心律失常大鼠心肌组织乳酸代谢、Ca2 浓度、脂质过氧化损伤及心肌细胞膜Na -K -ATPase和Ca2 -Mg2 -ATPase活性的影响.结果心律失常大鼠心肌组织乳酸大量堆积,脂质过氧化增强,Ca2 含量增高,而心肌细胞膜Na -K -ATPase、Ca2 -Mg2 -ATPase活性明显降低;与模型组比较,地精子皂甙大、中剂量治疗组心肌组织乳酸含量明显降低,心肌组织丙二醛(MDA)含量也明显较低,而超氧歧化酶(SOD)活性升高(P＜0.05),其中地精子皂甙大、中剂量和维拉帕米皆可明显提高心肌细胞膜Na -K -ATPase、Ca2 -Mg2 -ATPase活性,并能明显降低心肌组织Ca2 含量(P＜0.05).结论地精子皂甙具有明显抗心律失常的作用,其作用机制是通过改善缺血再灌注心肌乳酸代谢,对抗脂质过氧化损伤,提高缺血心肌细胞ATP含量,提高心肌细胞膜Na -K -ATPase、Ca2 -Mg2 -ATPase活性,抑制了Ca2 超负荷,从而发挥其抗心律失常的作用.     

冷凝集对血细胞分析多项参数检测结果的影响

目的 探讨红细胞冷凝集对血细胞分析各项检测参数的影响及处理方法.方法 选择福建省肿瘤医院2017年12月至2020年2月有红细胞冷凝集的全血标本73份.应用Sysmex XN-9000全自动血细胞分析仪对标本进行检测,比较37℃水浴前后红细胞(RBC)、血细胞比容(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白...     

Platelet and erythrocyte Mg2+, Ca2+, Na+, K+ and cell membrane adenosine triphosphatase activity in essential hypertension in blacks.

OBJECTIVE: To assess the relationship between intracellular Mg2+, Ca2+, Na+ and K+ and cell membrane adenosine triphosphatase (ATPase) activity in normotensive and hypertensive blacks. DESIGN: Intracellular cations and cell membrane ATPase activity were studied in black patients with untreated essential hypertension and age-, weight- and height-matched normotensive controls. Platelet, erythrocyte and serum Mg2+, Ca2+, Na+ and K+ levels as well as platelet and erythrocyte membrane Na+,K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase activities were measured in all subjects. METHODS: Intracellular Na+ and K+ were measured by flame photometry and Mg+ and Ca+ by atomic absorption spectrophotometry. Cell membrane ATPase activity was determined by a colorimetric method. RESULTS: The hypertensive group consistently demonstrated depressed activity of each ATPase studied, with significantly lower serum Mg2+, serum K+, erythrocyte Mg2+ and platelet Mg2+ levels compared with the normotensive group. Platelet Na+ and Ca2+ and erythrocyte Ca2+ were significantly elevated in the hypertensive group. In the hypertensive group, mean arterial pressure (MAP) was inversely correlated with platelet and erythrocyte membrane Na+,K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase. Serum Mg2+, serum Ca2+ and platelet Mg2+ were negatively correlated with MAP in the hypertensive group whilst erythrocyte and platelet Ca2+ were positively correlated. In the normotensive group, platelet Mg2+ and MAP were negatively, and erythrocyte Ca2+ and MAP, positively correlated. CONCLUSIONS: Black patients with essential hypertension have widespread depression of cell membrane Na+,K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase activities with serum and intracellular Mg2+ depletion and cytosolic Na+ and Ca2+ overload, which may reflect an underlying membrane abnormality in essential hypertension. These cellular abnormalities may be related to the defective transport mechanisms that in turn may be aggravated by Mg2+ depletion.     

Sample stability for complete blood cell count using the Sysmex XN haematological analyser

Background

Sample stability is a crucial aspect for the quality of results of a haematology laboratory. This study was conducted to investigate the reliability of haematological testing using Sysmex XN in samples stored for up to 24 h at different temperatures.

Materials and methods

Haematological tests were performed on whole blood samples collected from 16 ostensibly healthy outpatients immediately after collection and 3 h, 6 h or 24 h afterwards, with triple aliquots kept at room temperature, 4 °C or 37 °C.

Results

No meaningful bias was observed after 3 h under different storage conditions, except for red blood cell distribution width (RDW) and platelet count (impedance technique, PLT-I) at 37 °C. After 6 h, meaningful bias was observed for mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) at room temperature, red blood cell (RBC) count, mean corpuscular haemoglobin concentration (MCHC), MCH, MCV and PLT-I at 4 °C, and RBC, RDW, MCHC, MCH and PLT-I at 37 °C. After 24 h, a meaningful bias was observed for MCHC, MCV, platelet count (fluorescent technique, PLT-F) and mean platelet volume (MPV) at room temperature, MCHC, MCV, PLT-I and MPV at 4 °C, and all parameters except RBC count and MPV at 37 °C.

Discussion

Great caution should be observed when analysing results of haematological tests conducted more than 3 h after sample collection.     

Characteristics of red blood cell populations fractionated with a combination of counterflow centrifugation and Percoll separation.

Red blood cell (RBC) fractions were studied after separation of whole blood by means of counterflow centrifugation, Percoll column (Pharmacia, Uppsala, Sweden), and a combination of both separation techniques. Mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), and hemoglobin A1c (HbA1c) were measured in each fraction. From the results it was obvious that the combination of both techniques was the best separation technique of these three. MCV had a good correlation with cell age as measured with HbA1c concentration gradient; MCH and MCHC less so. MCV and MCH decreased in parallel to an increase in HbA1c. MCHC increased with increasing HbA1c. From these data it is concluded that there is a steadily ongoing loss of cellular hemoglobin and proportionally more cellular water during the life of the RBC.     

Correlations between changes in hematological indices of mothers with preeclampsia and umbilical cord blood of newborns

Preeclampsia is a condition that might severely impact the health of mothers and their newborns. The aim of this investigation is to examine hematological parameters in mothers with preeclampsia and umbilical cord blood. Eighty preecalmptic mothers were recruited in the study. In addition, eighty normal pregnant mothers served as controls. Hematological parameters that include hemoglobin (Hb), red blood cell count (RBC), red cell distribution width (RDW), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell counts (WBC), platelet counts, mean platelet volume (MPV) and Platelet large cell ratio (PLCR) were examined. Results showed a strong association between preeclampsia and low birth weight, premature/cesarean delivery and proteinuria (P < 0.001). Hb and neutrophils were significantly lower (P < 0.01), whereas RDW, PCV, MCV, MCH, MCHC and lymphocytes were significantly higher than normal ones (P < 0.01). When cord blood of preeclamptic mothers were compared with that of normal ones, similar findings were observed. In addition, results showed significant and positive correlations between preeclamptic mothers and their newborn in Hb (r2 = 0.075, P < 0.05), PCV (r2 = 0.084, P < 0.01), MCV (r2 = 0.077, P < 0.05), MCHC (r2 = 0.115, P < 0.01), RBC (r2 = 0.086, P < 0.01) and retics (r2 = 0.306, P < 0.01). In conclusion, changes in several hematological parameters associated with preeclampsia were correlated in affected mothers and their newborns. Such biomarkers can be used to predict pregnancy outcomes in women with preeclampsia.     

Effects of C-peptide on microvascular blood flow and blood hemorheology

Beside functional and structural changes in vascular biology, alterations in the rheologic properties of blood cells mainly determines to an impaired microvascular blood flow in patients suffering from diabetes mellitus. Recent investigations provide increasing evidence that impaired C-peptide secretion in type 1 diabetic patients might contribute to the development of microvascular complications. C-peptide has been shown to stimulate endothelial NO secretion by activation of the Ca2+ calmodolin regulated enzyme eNOS. NO himself has the potency to increase cGMP levels in smooth muscle cells and to activate Na+K+ATPase activity and therefore evolves numerous effects in microvascular regulation. In type 1 diabetic patients, supplementation of C-peptide was shown to improve endothelium dependent vasodilatation in an NO-dependent pathway in different vascular compartments. In addition, it could be shown that C-peptide administration in type 1 diabetic patients, results in a redistribution of skin blood flow by increasing nutritive capillary blood flow in favour to subpapillary blood flow. Impaired Na+K+ATPase in another feature of diabetes mellitus in many cell types and is believed to be a pivotal regulator of various cell functions. C-peptide supplementation has been shown to restore Na+K+ATPase activity in different cell types during in vitro and in vivo investigations. In type 1 diabetic patients, C-peptide supplementation was shown to increase erythrocyte Na+K+ATPase activity by about 100%. There was found a linear relationship between plasma C-peptide levels and erythrocyte Na+K+ATPase activity. In small capillaries, microvascular blood flow is increasingly determined by the rheologic properties of erythrocytes. Using laser-diffractoscopie a huge improvement in erythrocyte deformability could be observed after C-peptide administration in erythrocytes of type 1 diabetic patients. Inhibition of the Na+K+ATPase by Obain completely abolished the effect of C-peptide on erythrocyte deformability. In conclusion, C-peptide improves microvascular function and blood flow in type 1 diabetic patients by interfering with vascular and rheological components of microvascular blood flow.     

Effects of hydroxyurea on hemoglobin F and water content in the red blood cells of dogs and of patients with sickle cell anemia.

A rationale for clinical trials of hydroxyurea (HU) treatment in sickle cell disease is that the agent increases red blood cell (RBC) fetal hemoglobin content. However, an additional effect of HU is to raise the mean corpuscular volume (MCV). To investigate the action of HU in a species that makes no electrophoretically distinguishable fetal hemoglobin, we treated dogs with the drug and compared their response to that of five patients with sickle cell anemia. Both dogs and patients had an increase in MCV, but the effect of HU treatment on the mean corpuscular hemoglobin concentration (MCHC), density, and water content of the RBCs differed in the two species. The dog RBCs became low in MCHC, high in ion and water content, and low in mean density. Thus, HU can raise MCV and lower MCHC without influencing fetal hemoglobin synthesis. A different pattern was seen in the sickle cell patients during HU treatment. Although the MCV of their RBCs increased, there was no change in MCHC, ion content, or mean density. A notable change in the sickle cell patients' blood was that two subpopulations of cells were nearly eliminated during HU treatment; the hypodense reticulocyte fraction and the hyperdense fraction that contains irreversibly sickled cells. These findings lead us to suggest that trials of HU in sickle cell disease must recognize the possibility that any beneficial effect of this agent might be due not only to an increase in hemoglobin F alone, but perhaps also to the associated increase in MCV or the altered RBC density profile.     

Effect of bioactive compounds extracted from euphorbious plants on hematological and biochemical parameters of Channa punctatus

Channa punctatus was exposed to four different concentrations of Rutin, Taraxerol and Apigenin. Changes in some hematological parameters of Channa punctatus were assessed to determine the influence of these compounds on test fish. Fish were exposed to sublethal concentrations (80% of LC50 of 24h) of these compounds for one week. Control fish were also administered for one week. Thereafter, blood samples were obtained from the control and experimental fish. Blood was assayed for selected hematological parameters (hematocrit, hemoglobin, red blood cell count, white blood cell count total plasma protein and plasma glucose concentration). The derived hematological indices of mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were calculated. Sublethal concentrations of these compounds caused a dose dependent decrease in hemoglobin values coupled with a decrease in hematocrit values and red blood cell counts are an obvious indication of anemia. The total white blood cell counts and the differential white blood cell counts were decreased except for the lymphocytes, where there was a slight increase. Plasma protein and glucose were also lower in exposed fish when compared with control. The hematological indices MCH, MCHC, MCV were also lowered. The result from this study reveals high mortality rate and deleterious consequences on the health of fish subjected to acute exposure of Rutin, Taraxerol and Apigenin and therefore, should not be used directly in aquaculture without having the proper knowledge.     

高血压病人红细胞ATPase,细胞离子和游离钙的变化

本研究对 39例轻、中度高血压病 (EH)患者 ,通过皂素溶血定磷法测定红细胞ATPase活力 ,原子吸收火焰法测定红细胞离子含量 ,Fura 2 /AM荧光探针测定红细胞 [Ca2 ]i含量 ,并以正常人作对照 ,旨在探讨部分EH的发病机制。结果显示 ,EH组红细胞Ca2 ATPase活力明显降低 ,P <0 .0 1,而Na K ATPase无显著性差异 ,两者之间呈正相关 (r =0 .46 ) ;红细胞Na 和 [Ca2 ] i含量 ,均显著增高 (P <0 .0 1和 0 .0 0 1) ,而细胞K 含量明显降低 ,以致Na /K 比值增大 (均P <0 .0 1)。研究表明 ,EH患者红细胞高Na ,高 [Ca2 ] i及低K ,是膜缺陷及阳离子转运异常的标志或结果 ,是部分EH患者发病的病理基础和分子生物学基础。红细胞Na 泵活力正常 ,Ca2 泵活力抑制 ,可为EH的一种临床类型     

内源性类洋地黄物质对高血压病患者左室舒张功能的影响

测定 2 0例正常人和 2 0例高血压病患者的心尖搏动图指数、血清内源性类洋地黄物质 （EDL S）浓度、红细胞膜 Na+ - K+ - ATP酶 （钠泵 ）活性及红细胞内钾、钠、钙总量。结果显示 ,与正常人比较 ,高血压病患者的房缩波与E- O垂直高度比值 （A/E- O）明显增高 ,等容舒张期 （IRT）明显延长 ,血清 EDL S浓度明显升高 ,红细胞膜钠泵活性下降 ,红细胞内钠、钙含量升高、钾含量下降 ,且血清 EDL S浓度与 A/E- O,IRT呈显著正相关。提示 EDL S可能参与高血压病患者左室舒张功能异常的形成 ,其机制可能与 EDL S抑制心肌细胞膜钠泵活性并导致细胞内 Ca2 +含量升高有关