Novel HLA-DRB1 alleles discovered during routine sequencing based typing,DRB1*03052, DRB1*04032, DRB1*1139 and DRB1*1346

We report the discovery of four HLA-DRB1 alleles during routine sequencing based typing (SBT). These alleles--DRB1*03052, DRB1*04032, DRB1*1139 and DRB1*1346--differ from previously identified DRB1 alleles by known nucleotide polymorphisms.     

A novel HLA-DRB1 allele, DRB1*0832, identified by sequence-based typing method

Human leukocyte antigen-DRB1*0832 may have arisen by gene conversion from a DRB1*080302 backbone, leading to amino acid changes at codons 26, 28, 30, 32, 37, and 38.     

Novel HLA-DRB1 alleles revealed by sequencing based typing,DRB1*04053 and DRB1*1143

We report the discovery of two HLA-DRB1 alleles by sequencing based typing (SBT). DRB1*04053 differs from previously reported DRB1 alleles by a single synonymous nucleotide substitution, resulting in a unique polymorphism at codon 93. DRB1*1143 differs from previously identified DRB1 alleles by a single non-synonymous nucleotide substitution, resulting in a polymorphism observed in other DRB1 and DRB3 alleles1.     

Identification of two new HLA-DRB1 alleles,DRB1*1138 and DRB1*1344

Two new HLA-DRB1 alleles have been identified by sequencing based typing (SBT). HLA-DRB1*1138 and DRB1*1344 were discovered after following up ambiguous results involving unusual alleles after DRB1 generic typing.     

A new DRB1*15 allele (DRB1*1506) identified by sequence-based typing

A new DRB1*15 allele (DRB1*1506) was detected during the studies of the 12th International Histocompatibility Workshop within the Allele and Haplo-type Society #11 which studied the DR2 and DR51 antigens. The new allele was found in four unrelated Asian Indian individuals by sequence-based typing. It has a base substitution from T to C in codon 50 at a previously considered conserved position.     

A novel HLA-DRB1 allele, DRB*090202, is identified in a Taiwanese family

Human leukocyte antigen-DRB1*090202 has two synonymous nucleotide substitutions with DRB1*090201 at codons 57 and 58, which may be the result of a gene conversion.     

Description of a novel HLA-DRB1 allele,DRB1*1207

A new DRB1 allele has been identified during routine HLA-DRB1 generic typing with sequence specific oligonucleotides. The new allele was confirmed by PCR-restriction fragment length polymorphism analysis and subsequently sequenced. This showed the novel allele, named HLA-DRB1*1207, was similar in sequence to DRB1*12011, except for codon 69 of exon 2.     

Exon 2 sequence analysis of a novel HLA-DRB1 allele, DRB1*0314

We describe the identification of a new DRB1*03 variant in a Caucasian volunteer bone-marrow donor (DR'KW'). The indication for the existence of this new variant arose from contradictory results obtained by serological and several DNA typing methods (SSP, SSOP). Analysis of the exon 2 sequence revealed that the DRB1*0314 contained the characteristic DR3 specific sequence motifs, except for codon 77 (AAC-->ACC) where threonine is substituted for asparagine.     

Description of a new HLA-DRB1 allele, DRB1*1150

We report the identification of a novel HLA-DRB1*11 using sequence-based typing. This new allele, officially named DRB1*1150, was detected while performing HLA-DRB1 high-resolution typing of a candidate for bone marrow transplantation. DRB1*1150 is identical to DRB1*1143 except at nucleotides 200 and 227, where a T is substituted by a C and a T by an A, respectively. These differences give rise to two amino acid substitutions at codons 38 (Val-->Ala) and 47 (Phe-->Tyr).     

A novel HLA-DRB1 allele, DRB*1611, is identified in two Taiwanese individuals

Human leukocyte antigen (HLA)-DRB1*1611 has one nucleotide change at codon 14 (GAG→AAG) from DRB1*160201, resulting in a coding change from Glu to Lys.     

Novel HLA-DRB3 alleles discovered during routine sequencing based typing,DRB3*02023, DRB3*0212, DRB3*0213 and DRB3*03012

We report the discovery of four HLA-DRB3 alleles during routine sequencing based typing (SBT); DRB3*02023, DRB3*0212, DRB3*0213 and DRB3*03012. These alleles differ from other HLA-DRB3 alleles by previously undescribed single nucleotide polymorphisms.     

A novel HLA-DRB1 sequence, DRB1*11272

We describe the complete exon 2 sequence of a novel HLA-DRB1 allele, DRB1*11272. This allele differs from the DRB1*11271 allele by a synonymous mutation in codon 77 where an AAT is replaced with AAC, both encode for the amino acid asparagine. The same motif at codon 77 has also been found in DRB1*1107, DRB1*1333, DRB1*0422 and in most DRB1*03 alleles. A partial exon 2 sequence of this allele has previously been deposited in the EMBL Sequence Database under the accession number AF186407.     

Report of a new DRB1*13 allele: DRB1*1336

This brief communication describes the characterization of a new allele, DRB1*1336.     

A novel HLA-A*02 allele, A*02202, identified by nucleotide sequencing

In this paper we report the identification of a new HLA-A*02 allele in two members of an Afghan family. This novel allele, designed as A*02202, differs from A*02201 by a silent substitution at codon 66 (AAC-->AAT) in the alpha1 domain. A*02202 appears to be the result of a novel mutation (Note).     

Identification of a novel HLA-DRB1 allele, DRB1*116502, by sequence-based typing in an African-American individual

The novel allele HLA-DRB1*116502 differs from HLA-DRB1*110201 by nucleotide substitutions at codon 59 (GAG→GAC) and 93 (CGG→AGG).