幽门螺杆菌感染与慢性胃炎红细胞免疫功能变化的关系

目的：探讨幽门螺杆菌感染与慢性胃炎患者红细胞免疫功能的影响。方法：采用红细胞C35受体花环（RBC-C3b RR）试验、免疫复合物花环（RBC-ICR）试验、免疫粘附促进因子（RFER）百分率及抑制因子（RFIR）百分率试验,检测315例慢性胃炎患者与30例健康体检者红细胞免疫功能情况。结果：慢性胃炎组RBC-C3bRR、RFER明显低于对照组（P＜0.01,R＜0.05),RBC-ICR、RFIR高于对照组（P＜0.05,P＜0.01);Hp阳性组RBC-C3bRR明显低于Hp阴性组（P＜0.05),RBC-ECR明显高于HP阴性组（P＜0.05),RFER、RFIF两组间比较未见显著差异;对186例Hp阳性根除治疗前、后红细胞免疫功能对比发现治疗后RBC-ICR明显低于根除前,其余指标无显著改变。结论：Hp的感染可能导致宿主红细胞免疫功能紊乱,其感染可能是慢性胃炎患者红细胞免疫功能下降的主要原因。     

幽门螺杆菌感染与慢性胃炎红细胞免疫功能变化的关系

目的 :探讨幽门螺杆菌感染与慢性胃炎患者红细胞免疫功能的影响。方法 :采用红细胞C3b受体花环 (RBC -C3bRR)试验、免疫复合物花环 (RBC -ICR)试验、免疫粘附促进因子 (RFER)　百分率及抑制因子 (RFIR)百分率试验 ,检测 3 15例慢性胃炎患者与 3 0例健康体检者红细胞免疫功能情况。结果 :慢性胃炎组RBC -C3bRR、RFER明显低于对照组 (P <0 0 1,P <0 0 5 ) ,RBC -ICR、RFIR高于对照组 ((P <0 0 5 ,P <0 0 1) ;Hp阳性组RBC -C3bRR明显低于Hp阴性组 (P <0 0 5 ) ,RBC -ECR明显高于HP阴性组 (P <0 0 5 ) ,RFER、RFIF两组间比较未见显著差异 ;对 186例Hp阳性根除治疗前、后红细胞免疫功能对比发现治疗后RBC -ICR明显低于根除前 ,其余指标无显著改变。结论 :Hp的感染可能导致宿主红细胞免疫功能紊乱 ,其感染可能是慢性胃炎患者红细胞免疫功能下降的主要原因。     

烧伤患者红细胞免疫功能的变化

目的　了解烧伤患者红细胞免疫功能的变化及其特点。方法　采用酵母花环试验测定了 5 2例烧伤患者及 40例健康体检者RBC C3 bRR和RBC ICR这两项红细胞免疫功能的主要指标。结果　严重烧伤患者RBC C3 bRR较对照组明显降低 ,而RBC ICR则明显升高。从而表明在烧伤患者体内 ,红细胞的免疫粘附功能低下。结论　在对烧伤的临床治疗过程中 ,我们不仅要调节白细胞免疫系统的功能 ,改善患者红细胞免疫功能也是十分必要的。     

糖尿病患者红细胞免疫功能及一氧化氮的变化

本文报道了６０例糖尿病患者红细胞免疫粘附功能与一氧化氮（ＮＯ）的检测结果，并与３８例正常人进行比较，其红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）明显低于正常人，红细胞免疫复合物花环率（ＲＢＣ－ＩＣＲ）明显高于正常人（Ｐ〈０．００１，Ｐ〈０．００１），胰岛素依赖型糖尿病（ＩＤＤＭ）患者尿ＮＯ水平明显高于正常人（Ｐ〈０．００１），提示通过糖尿病患者红细胞免疫粘附功能ＮＯ的测定可了解与感染的关系及为感     

急性心脑血管疾病患者红细胞免疫功能的改变

目的探讨急性心脑血管疾病患者(ACCVD)红细胞(RBC)免疫功能的改变。方法采用免疫粘附酵母菌花环法。结果ACCVD组与健康组相比,红细胞C3b受体花环(RBC-c3bRR)率与红细胞免疫粘附促进因子(RFER)百分率明显降低(P<0.01),红细胞免疫复合物(RBC-ICR)花环率与红细胞粘附抑制因子(RFIR)百分率明显增加(P<0.01)。结论ACCVD患者红细胞免疫功能较健康人低,红细胞免疫功能低下亦可能是血栓形成的原因之一。     

急性脑梗塞患者的红细胞免疫功能和NO含量变化

人们己认识到 ,免疫机制参与了脑梗塞的发生发展过程。大量资料证实 ,脑梗塞与红细胞免疫有密切关系。ＮＯ在脑梗塞中的作用 ,也日益引起人们的关注。本文对 35例急性脑梗塞患者进行了红细胞免疫粘附功能测定 ,并对其中 2 4例进行了ＮＯ含量测定。1　资料和方法1.1　临床资料　 35例患者 ,均按第二次全国脑血管病学会议第三次修订标准确诊。并经头颅ＣＴ及 /或ＭＲＩ证实。男 2 4例 ,女 11例 ,年龄 47～ 82 (平均 6 5 .8± 8.8)岁 ,病程均为一周内。对照组 2 0例 ,选自参加体检的健康人 ,男 14例 ,女 6例 ,年龄 45～ 71(平均 6 0 .3± 9.0 )…     

妊高征红细胞免疫功能变化的研究

目的：探讨妊高征患者红细胞免疫功能的变化及在妊高征发病中的作用。方法：４０例妊高征患者采用红细胞Ｃ３ｂ受体花环试验（ＲＣ３ｂＲ）,红细胞免疫复合物花环试验（ＲＩＣＲ）及测定循环免疫复合物含量。并与正常孕妇进行对照,并以部分病例进行跟踪检测。结果：妊高征组（ＲＢＣＣ３ｂ）明显低于对照组差异有显著性（Ｐ〈０．０５）,随病情加重逐渐呈下降趋势,ＲＩＣＲ高于对照组,差异有显著性（Ｐ〈０．０５）,其值随病情     

Characterization of circulating and cultured Tfh-like cells in sickle cell disease in relation to red blood cell alloimmunization status

BackgroundPeople living with sickle cell disease (SCD) are prone to red blood cell (RBC) alloimmunization. We hypothesized that subjects with alloantibodies (responders) would have differences in circulating T-follicular helper (Tfh)-like cells compared to subjects without alloantibodies (non-responders).Materials and methodsPeripheral blood mononuclear cells were collected from 28 subjects, including those with SCD and controls. Circulating CD4 T-cell subsets were first evaluated at baseline. CD4 T-cell subsets were also evaluated after naïve CD4 T-cells were differentiated into Tfh-like cells following in vitro culture with CD3/CD28 beads, IL-7, IL-12, and Activin A. Transfusion and alloantibody histories were extracted from the electronic medical record.ResultsNon-responders had a lower percentage of CD45RA negative Tmemory cells than responders or controls (p<0.05). Notably, there were no differences in circulating Tfh-like cells between any group. However, naïve CD4 T-cells from subjects with SCD were more likely to express CXCR5 after in vitro culture than cells from controls. After culture, CXCR5 expressing cells from responders were more likely to express PD1 and ICOS (16.43 %, sd. 20.23) compared to non-responders (3.69 %, s.d. 3.09) or controls (2.78 %, s.d. 2.04).DiscussionThe tendency for naïve CD4 T-cells from responders to differentiate into Tfh-like cells after in vitro culture may suggest these cells are prepared to assist B-cells with antibody production regardless of antigen specificity. Further studies are needed, but it is possible that these results may explain why some responders form RBC alloantibodies with multiple specificities, in addition to RBC autoantibodies and HLA alloantibodies.     

慢性胃炎与消化性溃疡患者幽门螺杆菌的感染状况及诊断方法

本文通过200例患者活检标本的对比研究证明,幽门螺杆菌在消化性溃疡与活动期慢性胃炎中有较高的感染率,分别为68%～92%和62.9%～92.6%;而非活动期慢性胃炎仅为36.6%～39.8%.同时证明,用pH试纸检测尿素酶的存在是一个经济、简易、快速、有较高特异性和敏感性的诊断幽门螺杆菌感染的方法.     

慢性胃炎的内镜表现

随着内镜设备的不断改进,慢性胃炎的诊断及早期胃癌的检出率得到有效提高。目前慢性胃炎的内镜诊断要点是通过内镜表现判定有无幽门螺杆菌（H.pylori）感染并评价胃癌发生的风险。由于多数慢性胃炎患者无明显临床症状,因此全面准确认识慢性胃炎的内镜表现尤为重要。本文根据有无H.pylori感染、药物所致及胃癌高风险黏膜的内镜表现依次述评,以期能够提高内镜对H.pylori感染状态及胃癌风险的诊断和分级能力,从而对实际临床诊疗有所裨益。     

胃溃疡合并慢性乙型肝炎患者胃黏膜产生免疫球蛋白的细胞活性的影响研究

目的 对胃溃疡合并慢性乙型肝炎(下称乙肝)患者的胃黏膜产生免疫球蛋白的细胞进行研究.方法 研究组为52例胃溃疡合并慢性乙肝患者,对照组为24例单纯胃溃疡患者.以尿素酶试验及组织Giemsa染色法检测幽门螺杆菌(Hp);以免疫组织化学SABC法检测胃黏膜免疫球蛋白产生细胞.结果 单纯胃溃疡患者无论Hp检测是否为阳性,胃黏膜免疫球蛋白产生细胞均以IgA产生细胞为主.而胃溃疡合并慢性乙肝患者,产生IgA细胞明显减少,合成IgM及IgG细胞明显增多,尤以Hp阳性患者为著.结论 胃溃疡合并慢性乙肝患者胃黏膜免疫状态发生了改变,对Hp的清除和溃疡的愈合可能产生不利的影响.     

海藻糖和蔗糖在人红细胞冰冻干燥保存中的效果比较

目的 比较海藻糖和蔗糖在人红细胞冰冻干燥保存过程中对细胞的保护效果。方法 将浓缩红细胞、含有不同浓度海藻糖或蔗糖的30％聚乙烯吡咯烷酮(PVP)按照1:3(V/V)的比例混合,-80℃冰箱中预冻1h,入冻干机内冻千处理后,用37℃等渗缓冲液快速水化洗涤样品,然后将其分为3个处理组:组1为30％PVP组(简称PVP组),组2、3为含有5％、10％和15％海藻糖或蔗糖的30％PVP组(简称海藻糖组或蔗糖组),测定各项指标。结果 冻干红细胞再水化后,海藻糖组的细胞回收率[(18.86±4.63)％、(21.73±7.32)％和(18.01±4.53)％]显著低于PVP组[(45.97±11.77)％](P<0.01);蔗糖组,蔗糖浓度为5％、10％时,细胞回收率分别为(37.40±2.90)％和(37.77±4.78)％,二者显著低于PVP组(P<0.05),但当蔗糖浓度升至15％时,细胞回收率[(42.54±10.25)％]和PVP组无显著差异;血红蛋白回收率,海藻糖组[(50.00±3.47)％、(40.91±9.09)％和(52.09±7.01)％]显著低于PVP组[(77.27±3.63)％](P<0.05),而蔗糖组和PVP组无显著差异,且显著高于海藻糖组(P<0.05)。洗涤后3组的游离血红蛋白浓度均<1g/L,其余各项指标的对比关系类似于水化后指标。结论 在冻干保护液中添加不同浓度的海藻糖或蔗糖对于提高冰冻干燥保存后红细胞的回收率和血红蛋白浓度作用不明显;但     

Unit-to-unit variability in the deformability of red blood cells

BackgroundIn blood banking practice, the storage duration is used as the primary criterion for inventory management, and usually, the packed red blood cells (PRBC) units are supplied primarily according to first-in-first-out (FIFO) principle. However, the actual functionality of individual PRBC units is mostly ignored. One of the main features of the RBCs not accounted for under this approach is the deformability of the red cells, i.e., their ability to affect the recipients' blood flow. The objective of the study was to analyze unit-to-unit variability in the deformability of PRBCs during their cold storage.MethodsRBC samples were obtained from twenty leukoreduced PRBC units, stored in SAGM. The deformability of cells was monitored from the day of donation throughout 42 days. RBC deformability was determined using the computerized cell flow-properties analyzer (CFA) based on cell elongation under a shear stress of 3.0 Pa, expressed by the elongation-ratio (ER). The image analysis determines the ER for each cell and provides the ER distribution in the population of 3000–6000 cells.ResultsThe deformability of freshly-collected RBCs exhibited marked variability already on the day of donation. We also found that the aging curve of PRBC deformability varies significantly among donors.SignificanceThe present study has demonstrated that storage duration is only one of the factors, and seemingly not even the major one, affecting the PRBCs functionality. Therefore, the FIFO approach is not sufficient for assessing the potential transfusion outcome, and the PRBC functionality should be determined explicitly for each unit.     

Gastroduodenal reflex in duodenal ulcer and chronic hyperacidic gastritis

AIM: To study motor and secretory function of the stomach and duodenum in duodenal ulcer (DU) and chronic hyperacidic gastritis (CHG). MATERIAL AND METHODS: Autonomic nervous system was studied and esophagogastroduodenoscopy, 24-h intragastric pH-metry were made in 42 DU and 20 CHG patients. RESULTS: In comparable secretion, the patients of the above groups differed by severity and frequency of duodenogastric reflux (DGR). Grave and moderate DGR was typical for CHG and DU in the absence of complications. Minimal DGR occurred in DU with complications. CONCLUSION: DGR severity may be of prognostic significance for DU course.     

小儿幽门螺杆菌感染与慢性胃炎

目的 :研究幽门螺杆菌 (Helicobacterpylori,Hp)感染与小儿不同类型慢性胃炎之间的关系。 方法 :对我院 2 0 0 0年—2 0 0 2年 14 0 4例具有上消化道症状 ,经胃镜检查确诊为慢性胃炎的患儿进行胃黏膜活检 ,分别行病理组织学检查及快速尿素酶试验、改良Giemsa染色找Hp ,同时患儿行血清抗HpIgG检测和 (或 ) 13 碳 尿素呼气试验 (13 C UBT)和 (或 )粪幽门螺杆菌抗原检测 (Helicobacterpyloristoolantigen ,HpSA)。 结果 :14 0 4例慢性胃炎患儿中 ,Hp感染率为 4 5 .3%。各种不同类型慢性胃炎中 ,以胃镜下结节性胃炎和消化性溃疡伴慢性胃炎的患儿Hp感染率为最高 ,分别为 71.2 %和 6 8.0 % ,明显高于其他胃炎组 (P <0 .0 1)。胃黏膜病理组织学改变 ,Hp感染组引起的黏膜炎症程度较重 ,淋巴滤泡形成比例明显较对照组高 (P <0 .0 1)。结论 :小儿时期Hp感染率已较高 ,随年龄增长Hp感染率增高。并且与小儿胃十二指肠疾病关系密切 ,其中与胃镜下结节性胃炎和消化性溃疡伴慢性胃炎最为密切。胃黏膜组织炎症程度越重 ,Hp感染阳性率越高。