屈光不正对微扫视性眼球运动的影响

目的观察屈光不正对人眼微扫视性眼球运动的影响。方法前瞻性病例对照研究。收集2010年10月至2011年3月在天津市眼科医院就诊的屈光不正患者17例和无屈光不正的受试者17例,按照屈光状态与眼别进行分组。屈光不正者未戴镜矫正条件下17只主导眼为ADa组,17只非主导眼为ANa组;屈光不正者戴镜矫正条件下17只主导眼为ADb组,非主导眼为ANb组;正常受试者主导眼为ND组,非主导眼为NN组。采用高速眼球运动记录系统对受试者双眼分别进行注视性眼球运动记录。采用自编的Matlab程序对微扫视性眼球运动成分进行识别、提取和分析。对各组微扫视幅度、峰值速度、发生频率、微扫视间隔时间等量化指标的组间比较采用单因素方差分析（ANOVA）,两两比较采用Turkey检验,以P〈0．05为差异有统计学意义。结果6组间平均微扫视幅度的差异无统计学意义,但ADa组（5．42％±0．26％）、ANa组（5．48％±0．25％）较ADb组、ANb组、ND组、NN组幅度变异度大,差异有统计学意义（F=38．67,P〈0．01）;ADa组[（55．25±2．40）°／s]、ANa组[（54．51±1．77）°／s]微扫视峰值速度较ADb组、ANb组、ND组、NN组小（F=311．84,P〈0．01）;ADa组[（1．56±0．03）Hz]、ANa组[（1．57±0．05）Hz]微扫视发生频率较ADb组、ANb组、ND组、NN组低（F=155．25,P〈0．01）;ADa组[（558±23）ms]、ANa组[（555±22）ms]微扫视间隔时间较ADb组、ANb组、ND组、NN组长（聘102．12,P〈0．01）;屈光不正者戴镜或不戴镜及正常受试者主导眼与非主导眼比较,各项指标差异无统计学意义。屈光不正者戴镜条件下与正常受试者比较,各项指标差异也无统计学意义。结论屈光不正可影响人眼微扫视性眼球运动的行为,表现为微扫视幅度变异度增加、峰值速度降低、发生频率降低及微扫视间隔时间延长。戴镜可矫正屈光不正患者微扫视的异常。     

米诺环素对糖尿病视网膜病变的作用机制研究进展

糖尿病视网膜病变（ｄｉａｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ,ＤＲ）是糖尿病最常见且最严重的眼部并发症之一,现已成为２０～７４岁人群致盲的首要原因。其病理改变主要包括早期的神经细胞凋亡以及后期新生血管形成。米诺环素作为一种半合成四环素类抗生素,除了具有抗菌、抗炎症反应外还有抗新生血管、保护神经细胞的作用,越来越多的研究着重于米诺环素对ＤＲ的抑制作用,本文通过文献回顾,对米诺环素抑制ＤＲ的作用机制及研究进展进行综述,并对米诺环素在ＤＲ的研究方向进行展望。     

红细胞增多症影响早产儿视网膜病变的临床观察

目的 观察红细胞增多症对早产儿视网膜病变(ROP)的影响.方法 回顾分析262例早产儿的临床资料.患儿中红细胞增多症46例,占17.56%;其中,男性27例,女性19例.无红细胞增多症216例,占82.46%;其中,男性155例,女性61例.有无红细胞增多症两组患儿在出生体重(t=0.730,P=0.466)、胎龄(t=1.603,P=0.110)、吸氧人数(χ1=0.04,P>0.90)、吸氧时间(t=1.225,P=0.223)、吸氧浓度(t=1.823,P=0.071)之间比较,差异均无统计学意义.所有受检早产儿均由有经验的服科医生采用双目间接检眼镜检查眼底,确定有无ROP并进行分期.回顾分析时,着重分析有无红细胞增多症与ROP发生和分期之间的相互关系.结果 262例早产儿中发生ROP 120例,占45.80%.其中,红细胞增多症组发生ROP 25例,占红细胞增多症患儿的54.34%;无红细胞增多症组发生ROP 95例,占无红细胞增多症患儿的43.98%.有无红细胞增多症两组ROP发生率比较,差异无统计学意义(χ2=1.64,P>0.1).120例ROP患儿中,ROP<3期者104例,占86.67%;≥3期者16例,占13.33%.25例发生ROP的红细胞增多症患儿中,ROP<3期者18例,占发生ROP的红细胞增多症患儿的72.00%;≥3期者7例,占发生ROP的红细胞增多症患儿的28.00%.95例发生ROP无红细胞增多症患儿中,ROP<3期者的86例,占发生ROP的无红细胞增多症患儿的90.53%;≥3期者9例,占发生ROP的无红细胞增多症患儿的9.47%.有无红细胞增多症两组间在<3期和≥3期ROP发生率之间比较,差异有统计学意义(x2=4.38P<0.05).120例ROP患儿中,阈值前病变106例,占88.33%;阈值及以上病变14例,占11.67%.25例发生ROP的红细胞增多症患儿中,阈值前病变19例,占发生ROP的红细胞增多症患儿的76.00%;阈值及以上病变6例,占发生ROP的红细胞增多症患儿的24.00%.95例发生ROP无红细胞增多症患儿中,阈值前病变87例,占发生ROP的无红细胞增多症息儿的91.58%;阈值及以上病变8例,占发生ROP的无红细胞增多症患儿的8.42%.有无红细胞增多症两组间阈值前病变和阈值及以上病变ROP发生率比较,差异无统计学意义(χ2=3.27,P>0.05).结论 红细胞增多症不影响ROP发生率,但可能影响ROP的严重程度.     

干眼对生活质量影响的相关研究进展

干眼是一种日益严重的公共卫生问题,临床常见的干眼引起的眼部不适包括眼部干涩、视疲劳、眼异物感、烧灼感及畏光等,进一步可导致视觉损害和对比敏感度降低.这些不适很大程度上影响了干眼患者的身体功能、社会功能、心理功能、日常活动及工作生产力等情况.但干眼的视觉障碍对患者生活质量的影响不易量化,本文中笔者结合现有相关文献,对干眼视觉障碍的评估方法 及其对生活质量的影响进行综述.旨在为临床工作提供指导.     

眼位对主动追踪效果的实验影响

目的研究眼位对主动追踪效果的影响。方法以金属环模拟虹膜及瞳孔,其上置3.5 mm厚透明PMMA板模拟角膜,将该模拟装置置于MEL-70准分子激光主动追踪系统下,分别标记模拟装置在中央位置及位置偏移5 mm及10 mm后主动追踪系统所指示切削中心位置,观测两种位置偏移引起的追踪效果偏差量。结果偏差量分别为0.15mm及0.25 mm。结论眼位对主动追踪有影响,这种影响对常规屈光手术是可接受的,对高阶像差的影响则不容忽视。     

眼睑恶性肿瘤延误诊治因素的临床分析

目的:探讨眼睑恶性肿瘤延误诊治的原因。方法:对35例患者的职业、病变部位、病变特点、病变时间和诊疗过程等方面进行临床分析。结果:约74%患者为农民和工人,病程时间长,平均3.6年,20%的患者发生肿瘤侵袭和转移,病变初起表现为米粒大结节或色素痣,均有肿块快速增大的病史,部分患者病变有破溃、出血、溃疡的过程。结论:户外劳作者是易患人群,就诊不及时,未能引起患者和首诊医师足够的重视是主要因素。早期正确治疗极为重要。     

Studies on the mechanism of action of timolol and on the effects of suppression and redirection of aqueous flow on outflow facility

Long-term use of drugs that suppress aqueous humor formation, such as timolol and dorzolamide, or that redirect aqueous humor outflow from the trabecular meshwork, such as prostaglandin F2alpha analogues, could cause underperfusion of the trabecular meshwork and a secondary decrease in outflow facility. We investigated the mechanism of suppression of aqueous humor formation by timolol in monkey eyes by measuring aqueous humor ascorbate levels. We also determined whether suppression of aqueous humor formation with and without redirection of aqueous humor away from the trabecular meshwork could lead to a subsequent reduction in outflow facility, and whether this reduction was correlated with increased fibronectin levels in anterior chamber aqueous humor. In cynomolgus monkeys, unilateral dose/aqueous humor formation response curves were generated for timolol, dorzolamide, and a combination of timolol + dorzolamide. Aqueous humor formation and/or outflow facility were measured in both eyes after approximately four days, four weeks and seven weeks of twice daily treatment with 3.5 microg timolol + 1.0 mg dorzolamide to one eye and 30% DMSO to the other. In some monkeys, 5 microg prostaglandin F2alpha-isopropyl ester (PG) was added to timolol + dorzolamide for 4-week treatments. Intraocular pressure and corneal endothelial transfer coefficients (k(a)) were also measured at four weeks. Aqueous humor fibronectin levels were determined in four monkeys after approximately 9.5 weeks of timolol + dorzolamide treatment. Aqueous humor formation, intraocular pressure, and aqueous humor ascorbate levels were also determined in rhesus monkeys at baseline and after a single unilateral topical administration of 25 microg timolol. Compared to baseline for the same eye, aqueous humor formation was significantly decreased in treated eyes at all doses of timolol and at 1.8 and 4 mg dorzolamide. Compared to the opposite control eye, aqueous humor formation was lower in treated eyes after 3.5 and 5 microg timolol and after all doses of dorzolamide. Aqueous humor formation after treatment with 3.5 microg timolol + 1.0 mg dorzolamide was decreased in treated vs. control eyes, after four days and was suppressed in both treated and control eyes after four weeks of treatment, but not when PG was added. There was no difference in k(a) values with or without the addition of PG. Intraocular pressure was significantly lower in both treated and control eyes vs. baseline after approximately 6.5 weeks treatment with timolol + dorzolamide when taken 2 hr after the last dose and after approximately 3.5 weeks treatment with timolol + dorzolamide + PG when measured 6 hr after the last dose. Outflow facility after treatment with timolol + dorzolamide was unchanged after four days, tended to be lower in the treated vs. control eyes after four and seven weeks, and was significantly lower in treated vs. control eyes after four weeks treatment with timolol + dorzolamide + PG (0.352 +/- 0.052 vs. 0.515 +/- 0.096 microl min(-1) mmHg(-1), p < or = 0.02). Both treated vs. control eye aqueous humor fibronectin levels were below the level of detection for our assay (0.01 microg ml(-1)). The 25 microg timolol dose decreased ipsilateral, but not contralateral intraocular pressure (12.6 +/- 1.7 vs. 15.2 +/- 0.9; p < 0.05) and aqueous humor formation (1.40 +/- 0.08 vs. 2.03 +/- 0.09 microg ml(-1), p < or = 0.01). There was no difference in anterior chamber ascorbate levels in treated vs. control eyes or compared to their respective baselines. Our findings indicate that timolol affects neither ciliary epithelial transport of ascorbate nor aqueous fibronectin levels. Our data also indicate that decreasing aqueous humor formation over a period of time can lead to reduction in outflow facility, particularly when combined with therapy that redirects aqueous from the trabecular meshwork. Future intraocular pressure-lowering therapies for glaucoma may better be directed at enhancing flow through the trabecular pathway as opposed to decreasing aqueous humor formation or rerouting aqueous humor away from the trabecular meshwork.     

Experimental Study on the Effect of Perfiuorodecalin on Rabbit Corneas

Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively,and the corneas were examined under the light microscope. Results: Perfluorodecalin droplets looking like "fish eggs" were found at about 1/4 ～1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection,and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocomeal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively.After injection of perfluorodecalin, endothelial cell density was sighificantly decreased (2789 + 192 vs. 2 341 +658, P ＜ 0.01) and corneal thickness was increased. Conclusions: Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction. Eye Sciemce 2001; 17:16 ～ 20.     

Exoerimental Study on the Effect of Perfluorodecalin on Rabbit Corneas

Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0. 05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope.Results: Perfluorodecalin droplets looking like "fish eggs" were found at about 1 /4 ~ 1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous me     

双氢青蒿素对棘阿米巴抑制作用的实验研究

目的 评价双氢青蒿素对棘阿米巴的体外抑制作用。设计 实验研究。研究对象 临床患者角膜分离的阿米巴虫株。方法 体外培养阿米巴虫株,加入不同稀释浓度（3.2%、1.6%、0.8%、0.4%、0.2%、0.1%、0.05%、0.025%和0.0125%）的双氢青蒿素（DHA）共同孵育,同时以洗必泰（CHG）和聚六甲基双胍（PHMB）做对照药物,通过转种阿米巴培养基和LIVE/DEAD染色观察药物对阿米巴活性的抑制作用。主要指标 光学显微镜观察药物作用后虫体形态变化;LIVE/DEAD染色后,荧光显微镜下阿米巴活性情况,计算最小杀包囊浓度（MCC）和最小杀滋养体浓度（MTAC）值,以及各MTAC和MCC水平下阿米巴虫株的累计分布率,即MTAC50或MCC50和MTAC90或MCC90。结果 药物抑制后的阿米巴包囊干瘪皱缩,LIVE/DEAD法观察,受抑制的包囊呈红色,无活性。DHA组MTAC和MCC值范围均为0.05%~1.6%。MTAC50和MCC50值均为0.4%,MTAC90和MCC90值均为1.6%;CHG组MTAC值范围0.0125%~1.6%,MCC值范围0.0125%~0.8%,MTAC50和 MCC50值分别为0.05%和0.1%,MTAC90和MCC90值均为0.8%。PHMB组MTAC值范围0.00625%~0.4%,MCC值范围0.00625%~0.2%,MTAC50和MCC50值均为0.025%,MTAC90和MCC90值均为0.2%。三组药物对不同阿米巴菌株的MCC和MTAC相比较均无统计学差异（P=0.342、0.459、0.469）。三组MTAC值总体比较DHA组最高,CHG组次之,PHMB组最低（F=3.813,P=0.035)。其中,DHA组高于PHMB组（P=0.011）;DHA组和CHG组以及CHG组和PHMB组比较差异无统计学意义（P=0.105和0.297）。三种药物组的MCC值总体比较差异有统计学意义（F=6.672,P=0.004), DHA组比CHG组高,DHA组比PHMB组高（P=0.017和0.001）; CHG组与PHMB组无差异（P=0.325）。结论 双氢青蒿素在体外对棘阿米巴有抑制作用,其临床治疗阿米巴性角膜炎的作用仍待验证。     

Effect of light on the eye on metabolism and hormones

Numerous metabolic parameters in serum and urine were examined in 110 cataract patients before and after cataract surgery. The marked reduction in light passing through the eye due to opacities (vision less than 1/10) leads to characteristic metabolic and hormonal disturbances. ACTH and cortisol production decreases, metabolism slows down and due to an adrenal insufficiency for which the pituitary is responsible there are characteristic changes in the cortisol-dependent metabolic processes. In addition, an "energetic action" of the light affecting the hypothalamus via the retino-hypothalamic pathways (the "energetic portion" of the visual pathway) was proved in patients who were blinded by cataract and had metabolic disturbances as a result. Postoperatively, after elimination of the lens opacities, the metabolism and hormones of the same patients returned to normal. As a result of restoration of exogenous light stimulation to the diencephalon-hypophysis system via the retinohypothalamic pathway ("energetic pathway" of the optic system) the metabolism and hormones returned to normal during the patients' stay in the hospital. These comparative investigations in the same patients before and after cataract extraction provide for the first time irrefutable scientific evidence of the influence of light via the eye on the human organism.     

rhEGF促进人结膜上皮细胞增殖的实验研究

目的：探讨rnEGF（recombinant buman epidermal growth factor)促进培养人结膜上皮细胞增殖的作用及最佳有效浓度,为提高眼表重建术后成功率寻找有效的治疗手段。方法：在传代人结膜上皮细胞中分别加入1μg/ml-100μg/ml浓度的rhEGF,于加药后第24、48、72、96h分别采用MTT法得出光吸收值以测定活性细胞数。结果：24hEGF各浓度组与对照组光吸收值无明显差异。48h50μg/ml、20μg/ml、10μg/mlEGF组光吸收值明显高于对照组,72h除100μg/ml组与其余EGF浓度组有显著性差异。结论：EGF对结膜上皮的增殖有明显促进作用,浓度为10－50μg/ml可以获得较好的效果。     

苦参碱抑制视网膜母细胞瘤细胞增殖的研究

目的 探讨中药成分苦参碱（Ma）对视网膜母细胞瘤细胞株（HXO-Rb44细胞株）增殖的抑制作用及相关作用机制，为中药综合治疗视网膜母细胞瘤提供实验依据。设计 实验性研究。研究对象 体外培养的HXO-Rb44细胞。方法 0．4mmol／L的Ma分别作用HXO-Rb44细胞12、24、48小时，流式细胞仪检测S期细胞百分比的变化；通过细胞免疫组织化学染色结合计算机自动图像分析技术，测量阳性细胞积分光密度（IOD）值，观察分析Ma对HXO-Rb44细胞内增生靶基因PCNA的调控。主要指标 S期细胞百分比（SPF）、积分光密度值（IOD）。结果 0．4mmol／L浓度Ma作用HXO-Rb44细胞后，S期细胞百分比下降（P均〈0．01），细胞内PCNA蛋白表达水平下降（P均〈0．01），并均呈时间依赖性。结论 Ma能够抑制HXO-Rb44细胞增殖。提示Ma有望成为治疗视网膜母细胞瘤的有效药物。     

纹眼线对于干眼症发病的影响

目的:研究纹眼线对于干眼症发病的影响。方法:选择127例患有干眼症的患者,其中46例纹过眼线,其余81例未纹过眼线。比较两组患者的初次发病年龄、泪膜破裂时间、Shirmer试验、结膜印迹细胞,以及纹眼线患者纹眼线与干眼症初次发病之间的间隔时间。结果:纹眼线患者纹眼线与发病的间隔时间平均为13.4a,且纹眼线患者干眼发病的年龄有所提前。两组患者其他的检查结果无统计学差异。结论:纹眼线会使干眼症的发病年龄提前,但却不会影响初期发病的严重程度。纹眼线对于干眼症的病程及治疗的影响仍需进一步探讨。     

晶状体损伤对视神经损伤后再生影响的研究

视神经损伤是眼科重要的致盲因素之一 ,在眼科临床中尚未找到有效的治疗方法 ,为此寻找促进神经修复与再生的有效手段成为现代神经生物领域的热点。最近研究认为 :损伤晶状体后可以成功地促进视网膜神经节细胞的存活和视神经轴突的再生。我们对该领域迄今为止的主要研究成果做一综述。     

复方中药制剂抑制成纤维化增生作用的实验研究

目的观察复方中药制剂对结膜成纤维细胞增生的抑制作用,为临床防治增生性玻璃体视网膜病变提供新的治疗方法.方法以体外培养的兔结膜成纤维细胞作为研究对象,对照组用地塞米松、5-氟尿嘧啶、氢化可的松处理,实验组用丹苓(颗粒)冲剂及其君药(丹参、石决明)处理.48 h后,用MTT法观察成纤维细胞增生情况及其细胞形态变化特点.结果丹苓(颗粒)冲剂和丹参在一定浓度范围内可抑制成纤维细胞增生,且成剂量依赖关系;石决明对成纤维细胞增生未见明显抑制作用.5-氟尿嘧啶、氢化可的松在一定浓度范围内可明显抑制成纤维细胞增生;地塞米松在低浓度时轻度刺激成纤维细胞增生,但在高浓度时抑制成纤维细胞的增生.丹苓(颗粒)冲剂和丹参作用48 h后,部分成纤维细胞未能贴壁且收缩成圆形.结论丹苓(颗粒)冲剂可有效抑制成纤维细胞增生,有望成为防治增生性玻璃体视网膜病变的新药物.     

ET-1的眼科研究进展

内皮素1(ET-1)是体内已知的作用力最强、持续时间最长的缩血管肽,目前在眼科有了较广泛深入的研究,本文就其在糖尿病视网膜病变等眼底疾病、青光眼、角膜疾病中的作用研究作一阐述.     

Visualized analysis of research on myopic traction maculopathy based on CiteSpace

AIM: To analyze the global scientific output concerning myopic traction maculopathy (MTM) and to summarize the research frontiers and hot topics of MTM related researches. METHODS: Data were collected for bibliometric and visualization analyses from Web of Science (WOS) Core Collection. Exported records were analyzed for titles, publication years, research institutions, journal names, authors, keywords, and abstracts using CiteSpace software version 6.1. RESULTS: A total of 839 related studies were analyzed, the publication volume increased annually, with Asia the most active region of MTM research. Optical coherence tomography angiography, optical coherence tomography, macular hole, high myopia, macular buckling were identified as the focus of the current research. Progression, association, classification and shape were identified as the major research frontiers. CONCLUSION: MTM is a major cause of visual loss in pathological myopic eyes. During the preceding 17y, the number of annual publications in MTM research increased gradually. Studies on the progression nature of MTM, genome-wide association study and proper classification of MTM might still be the frontiers of MTM researches.     

牛磺酸对链脲佐菌素-糖尿病性白内障干预机制初探

探讨不同浓度牛磺酸对链脲佐菌素(STZ)-糖尿病性白内障的干预机制。方法：100只雄性SD大鼠,随机平均分为5组,腹腔注射STZ溶液(55mg／kg体重)诱发糖尿病性白内障,牛磺酸治疗组大鼠每日腹腔注射1次牛磺酸(5mL／kg体重)。定期测定各组大鼠血糖浓度,实验结束时测定各组血清中甘油三酯等生化指标及胰岛素水平,用毛细管电泳仪检测房水及晶状体中牛磺酸含量。结果STZ组大鼠在诱发的第3周晶状体开始出现囊泡或轻度混浊,而牛磺酸明显抑制了牛磺酸治疗组早期白内障的发生。诱发的第4～12周,STZ组晶状体混浊度迅速加重,而4％或8％牛磺酸组则明显延缓了白内障的发生。4％和8％牛磺酸治疗组在STZ诱发的第4天、第4周、第8周(4％牛磺酸组)的血糖均显著低于STZ组,第12周牛磺酸治疗组与STZ组的血糖值无显著意义。4％牛磺酸组大鼠血清甘油三酯水平显著低于STZ组(P=0.004),8％牛磺酸组大鼠血清甘油三酯水平也明显低于STZ组(P=0.010)。8％牛磺酸治疗组大鼠的房水中牛磺酸浓度高于STZ组,差异有显著意义(P=0.036),其晶状体中牛磺酸浓度也高于STZ组,差异有非常显著意义(P=0.000)。结论牛磺酸对STZ．糖尿病性白内障的干预作用具有剂量依赖性,其干预机制不仅与其降低血糖、降低甘油三酯水平、改善脂质代谢有关,特别与其提高房水和晶状体中牛磺酸含量,使晶状体免受氧化损伤有重要关系。     

Studies on the influence of ochratoxin A on rat lenses

Summary The influence of ochratoxin A on enzyme activities and on the content of free adenine nucleotides and intermediates of giycolysis in rat lenses was investigated. For a period of eight weeks, five times weekly, albino Wistat rats received ochratoxin A in 0.1% NaHCO₃, dosed 1/100 LD₅₀, via stomach tube. All measurements were made at the end of the experiment. Decreased contents of ATP, G-6-P, sum of fructose, and F-6-P were observed, but pyruvic acid, AMP, and DAP increased. Relatively decreased activities of enzymes MDH, ALD, HK, GK, and PK were established. The influence of ochratoxin A on the carbohydrate metabolism of rat lenses with respect to tranparency is discussed. There were no significant differences in absolute lens weight in animals treated by ochratoxin A and controls.

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Zusammenfassung Der Einfluß von Ochratoxin A auf Enzymaktivitäten und den Spiegel der freien Adenin-Nucleotide und Glykolysestoffwechselmetabolite wurde in der Rattenlinse bestimmt. Wistar-Albinoratten erhielten für eine Zeitdauer von 8 Wochen an 5 Wochentagen mittels Magensonde Ochratoxin A in 0,l%iger Lösung von NaHCO₃, die Tagesdosis war 1/100 DL₅₀. Alle Bestimmungen wurden am Ende des Experiments durchgeführt. Der Gehalt an ATP, G-6-P Fructose und Fructose-6-Phosphat war erniedrigt, Brenztraubensäure, AMP und DAP waren erhöht. Die Enzymaktivitäten von MDH, ALD, HK, GK und PK waren erniedrigt. Der Einfluß von Ochratoxin A auf den Kohlehydratstoffwechsel in der Rattenlinse in Hinblick auf die Transparenz wird diskutiert. Keine signifikante Differenzen bestanden übrigens in bezug auf das absolute Linsengewicht.