改良SDMC-2液对离体犬肺保存-移植的研究

目的在山东医科大学心脏液2号（SDMC-2）的基础上，开发有利于肺保存的器官保存液。方法30条Beagle犬随机分为3组，每组供、受体各5条，供体左肺经3种保存液于4℃保存12h；然后行受体左肺移植，测定并比较再灌注后的平均肺动脉压力（MPAP）及动脉血氧分压（PaO2）；对保存末及再灌注后肺组织标本进行电镜观察。结果（1）保存-移植再灌注后，3组与正常对照组比较：MPAP升高，PaO2降低（P〈0．01）；超微结构均不同程度损伤。（2）改良SDMC-2液保存组再灌注后即刻、0．5、1h的MPAP无明显变化，均低于相应时间的低钾保存液（LPD液）、SDMC-2液保存组（P〈0．05）；PaO2则高于相应时间的LPD液、SDMC-2液保存组（P〈0．05）。电镜显示：改良SDMC-2液保存组肺组织超微结构损伤最轻。结论改良SDMC-2液具有优良的保存效果及抗再灌注损伤能力。     

抑肽酶-低钾-右旋糖酐保存液对兔肺缺血再灌注损伤的影响

目的探讨抑肽酶-低钾-右旋糖酐保存液对常温兔肺缺血再灌注损伤的影响。方法成年新西兰大白兔18只,体重0．9～1．5kg,雌雄不拘,随机分为3组（n=6）：对照组（C组）、低钾-右旋糖酐（LPD）组（L组）、抑肽酶＋LPD组（A组）。C组直接阻断左肺门,不灌注肺保存液,L组和A组阻断左肺动脉,待肺膨胀后分别经左肺动脉导管灌注LPD保存液或抑肽酶＋LPD保存液30ml/kg（含抑肽酶150kIU/ml）,灌注结束后阻断左肺静脉,在肺膨胀一半时阻断左主支气管,2h后依次开放左肺静脉、动脉和左主支气管,再灌注90min后处死动物取出左肺,测定肺组织丙二醛（MDA）含量及髓过氧化物酶（MPO）活性,计算肺组织干湿重比（D/W）,观察肺组织病理学变化；分别于缺血前及再灌注15、60、90min检测动脉血氧分压（PaO2）及血清肿瘤坏死因子-α（TNF-α）浓度。结果与缺血前比较,3组再灌注期间血清TNF-α浓度升高,PaO2下降（P＜0．01）；与C组比较,L组、A组再灌注期间肺组织MDA含量、MPO活性降低,D/W升高,血清TNF-α浓度降低,PaO2升高（P＜0．05或0．01）；与L组比较,A组肺组织MDA含量、MPO活性降低,D/W升高,血清TNF-α浓度降低,PaO2升高（P＜0．01）。A组肺组织水肿、渗出、损伤等病理变化较C组和L组减轻。结论抑肽酶-LPD保存液可减轻兔常温肺缺血再灌注损伤,改善肺功能。     

温度对犬肺灌注保存效果的影响

目的评价不同温度灌注和保存犬肺对肺保存效果的影响。方法将24只犬随机分为实验组和对照组两组,每组供、受体各6只。实验组以23℃低钾右旋糖酐(LPD)液灌注后切取右肺保存于10℃LPD液,对照组以4℃LPD液灌注后切取右肺保存于4℃LPD液,其后两组均行左肺移植术。比较两组供体肺组织不同时间段的髓过氧化物酶(MPO)活性、丙二醛(MDA)含量及湿/干重量比(W/D),比较两组受体犬移植前后不同时间肺血流动力学参数以及血气分析指标,并比较病理结果。结果与对照组相比,实验组肺组织灌注后各时间段的MPO活性、丙二醛含量及W/D较低(P0.05)。实验组受体犬移植后的肺毛细血管楔压(PCWP)、平均肺动脉压(MPAP)较低,动脉血氧分压(PaO2)较高,差异均有统计学意义(均为P(0.05)。病理学结果显示,实验组肺组织中性粒细胞浸润、肺泡水肿及损伤等病理变化较对照组减轻。结论在本实验条件下,与4℃灌注+4℃保存相比,23℃灌注+10℃保存的温度对犬肺具有较好的保存效果,更适用于实验肺移植。     

非心脏跳动的供体肺脏在自制改良保存液中的保存效果

目的 评价LPD液,EC液和FWM液的肺脏保存效果.方法 18只纯种新西兰白兔随机分为3组:LPD组、EC组和FWM组.肺脏经过1 h的热缺血和4 h的低温(4℃)保存后,置于通气、全血灌注的离体兔肺模型上灌注1 h,每15 min做1次血气分析,再灌注结束后测量肺泡表面活性物质的活性及肺脏的干湿比.结果 FWM组中的肺脏氧合明显的高于其他组(P《0.05).再灌注后,FWM组的表面活性物质保存较好(P《0.05).干湿比在各组之间差异无统计学意义,但是FWM组显示出了水肿减轻的趋势.结论 FWM保存液在非心脏跳动的供体肺的保存中比LPD液和EC液有更好的保存效果.     

利多卡因对离体肺再灌注损伤保护作用的实验研究

目的通过离体肺再灌注模型观察利多卡因对肺再灌注损伤的保护作用.方法将12只兔随机分为两组Ⅰ组,低钾右旋糖酐(LPD)液灌洗兔肺后10℃保存,18 h后复灌;Ⅱ组,含利多卡因的LPD液灌洗后10℃保存,18 h后复灌.再灌注过程中测定氧分压(PO2)、二氧化碳分压(PCO2),灌注结束后测定肺组织湿干比(W/D)、髓过氧化物酶(MPO)活性以及支气管肺泡灌洗液(BALF)内中性粒细胞(PMN)计数,并观察肺组织形态学改变.结果Ⅱ组肺气体交换功能[PO2为(11.33±1.14)kPa(kPa=7.5 mm Hg)]、W/D(7.31±0.59)及BALF内PMN计数(0.39±0.05)情况显著优于Ⅰ组[(6.64±0.90)kPa、5.79±0.44、6.07±0.71],Ⅱ组MPO活性低于Ⅰ组,形态结构改变则明显优于Ⅰ组.结论灌洗液中加入利多卡因能明显改善肺气体交换功能,减少肺泡腔和间质内中性粒细胞浸润,减轻肺组织形态结构改变.     

硝酸甘油对移植犬肺的保护作用

目的评价硝酸甘油对犬肺的保护作用。方法将24只犬随机分为实验组和对照组两组,每组供、受体各6只。实验组用含硝酸甘油的低钾右旋糖酐(low potassium dextran,LPD)灌注液灌注供肺,对照组仅用LPD灌注液灌注供肺,留取供犬右肺继续于灌注液中保存至4h,修剪左肺作移植肺,两组受体犬均行左肺移植术。比较两组供体肺组织不同时间的髓过氧化酶(myeloperoxi-dase,MPO)活性、丙二醛(malondialdehyde,MDA)含量和湿/干重量比(W/D),比较两组受体犬移植前后不同时间肺血流动力学参数以及血气分析指标,并比较病理结果。结果与对照组相比,实验组肺组织灌注后各时间段的MPO活性、丙二醛含量均较低(均为P(0.05),两组W/D差异无统计学意义。实验组受体犬移植后的平均肺动脉压(mean pulmonary arterial pressure,MPAP)低于对照组而动脉血氧分压(PaO2)高于对照组(均为P(0.05)。移植后供肺肺组织的病理学结果显示,实验组肺组织水肿、炎性细胞浸润、损伤等病理变化较对照组减轻。结论在本实验条件下,在灌注液中加入硝酸甘油可以在犬肺移植过程中起到保护肺组织的作用。     

肺泡内不同氧浓度对供肺保存效果的影响

目的：研究低温保存期间肺泡内不同充气成份（纯氧和空气）对供者肺保存效果的影响。方法：实验分为3组,A组为新鲜正常对照组,B组为保存期间肺泡内充入空气组,C组为保存期间肺泡内充入纯氧组,将B,C两组的心肺组织浸于10度低钾右旋糖酐液中保存18h,取出后用温静脉血连续灌注左肺30min,结果：C组流出血氧分压（PO2）显著高于B组,与A组近似;二氧化碳分压（PCO2）显著低于A组和B组,肺动脉平均压明显低于A组和B组,再灌注后再二醛含量显著低于组,与A组近似。结论：供肺低温保存期间（18h)肺泡内充入纯氧的保护效果显著优于空气。     

异体脸面复合组织移植供体保存的研究

目的 探讨异体脸面部复合组织移植供体的有效保存方法，以减轻组织缺血缺氧损伤。方法 取狗的脸面部复合组织瓣，分别用4℃生理盐水和UW液灌注、保存，用MTT法检测复合组织瓣取下即刻、保存12、24、48h的各组织的活力，计算其与未保存组织相比的活力百分比，并进行病理学观察。结果 各种组织用UW液保存48h后活力仍为未保存组织的75％以上。保存后24、48h活力百分比与生理盐水组比较差异有统计学意义（P〈0．05）。病理切片示保存24、48h，生理盐水组皮肤、黏膜组织真皮层纤维排列疏松，轻度水肿，皮肤毛囊周围可见组织玻璃化，毛囊内可见细胞水肿，肌束间间隙扩大，腺体有部分腺泡细胞界限模糊，其他组织未见明显变化；UW液组各组织未见明显组织学变化。结论 UW液可以考虑用于临床异体脸面部复合组织瓣保存的保存液。     

抑肽酶对缺血肺组织ICAM-1和P-选择素基因mRNA表达的影响

目的 探讨抑肽酶对在体缺血冷存再灌注后肺组织细胞间黏附分子-1(ICAM-1)和P-选择素基因mRNA表达的影响。方法新西兰白兔30只，随机分为3组：对照组、PLD组和抑肽酶组。建立兔在体肺缺血冷存再灌注模型，对照组左肺不灌注肺保护液，阻断左肺门后直接将左肺下叶在体冷存于10℃肺保存器内2h，再灌注2h；IPD组左肺门阻断后经肺动脉灌注IPD液，余同对照组；抑肽酶组灌注液为含抑肽酶的改良IPD液，余同LPD组。分别于左肺门阻断前、缺血2h、再灌注2h取左肺组织。以RT-PCR技术检测P-选择素和ICAM-1基因mRNA表达。结果再灌注2h，对照组和IPD组P-选择素基因mRNA的表达量明显高于缺血前和缺血2h，抑肽酶组则无明显变化。缺血2h和再灌注2h。对照组和LPD组ICAM-1基因mRNA表达量较缺血前均显著升高，且明显高于抑肽酶组。结论抑肽酶可抑制缺血冷存再灌注后肺组织P-选择素和ICAM-1基因mRNA表达上调，有利于减轻肺组织缺血再灌注损伤。     

磷酸肌酸对离体大鼠肝脏冷保存的保护作用

目的探讨磷酸肌酸(CP)对大鼠离体肝脏冷保存的保护作用。方法建立大鼠肝脏单纯冷保存离体灌注模型,对照组予单纯威斯康星大学保存液(UW液)灌注肝脏,低剂量组以UW液为基液加入1 g/100 ml CP灌注肝脏,中剂量组以UW液为基液加入2 g/100 ml CP灌注肝脏;高剂量组以UW液为基液加入3 g/100 ml CP灌注肝脏。各组大鼠肝脏分别于4℃相应灌注液中冷保存后0、6、12、18、24 h共5个时间点,分别检测肝下下腔静脉内保存液的丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)含量,检测肝脏组织丙二醛(MDA)含量、髓过氧化物酶(MPO)活性,观察肝脏组织肝细胞的凋亡指数(AI)和肝脏组织核因子-κB阳性表达率,光学显微镜下观察肝脏组织的病理学变化。结果低、中、高剂量组大鼠肝脏在冷保存12 h后,ALT及LDH含量均低于对照组(均为P0.05);冷保存18 h后低、中、高剂量组大鼠肝脏组织的MDA、MPO含量均低于对照组(均为P0.05);在冷保存12 h及18 h时,低、中、高剂量组大鼠肝脏的肝细胞AI及核因子-κB阳性表达率均低于对照组(均为P0.05);冷保存24 h后,高剂量组保存液的ALT、MDA含量均明显高于对照组及低、中剂量组(均为P0.05)。病理检查结果显示,高、中、低剂量组大鼠肝脏的损伤明显轻于对照组,各剂量组之间比较无明显差别。结论在UW液中加入CP对大鼠离体肝脏冷保存有较好的保护作用,优于单纯应用UW液保存。     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.