汉防已甲素选择性PVC膜电极的研制

以四苯硼酸－汉防巳甲素离子缔合物性活性材料，制成了ＰＶＣ膜汉防已甲纱电极。对电影电极性能的诸因素进行了讨论，以最佳条件制备的电极其线性区为５．０×１０＾－３－－５．０×１０＾－６ｍｏｌ／Ｌ，平均斜率为２６．５ｍＶ／ｐＣ，检测下限为１．０×１０＾－７ｍｏｌ／Ｌ，在ＰＨ１．０－５．５范围内适用。电极抗干扰能力强，响应快，性和稳定性较好，地汉防已甲素制剂的快速测定。     

全固态石墨内导汉防己甲素电极的研制及应用

采用正交设计研究了在中性载体聚氯乙烯（ＰＶＣ）膜中，离子缔合物的种类、浓度及增塑剂３个因素对电极性能的影响。制备了性能稳定的石墨内导膜电极。在ｐＨ１．０～５．５之间电极电位基本稳定，线性范围为１．０×１０－２ｍｏｌ／Ｌ～２．０×１０－５ｍｏｌ／Ｌ，检测下限可达２．５×１０－６ｍｏｌ／Ｌ并能直接应用于原料药和制剂含量的测定。     

粉防己中汉防己甲素和乙素提取条件的优化

利用正交试验方法，研究了影响粉防己中汉防己甲、乙素的提取效率的各种因素，得到的最佳提取条件为：用含33．3％氨水的无水乙醇溶液浸泡8h，超声震荡提取4h。在此条件下测得的汉防己甲素的含量为0．678％，乙素的含量为0．616％。汉防己甲、乙素的含量用高效液相色谱（HPLC）检测，最低检出限分别为3．87×10-3和2．48×10-3mmol／L。     

汉防己甲素对肝硬化患者血清透明质酸及Ⅲ型前胶原肽含量的影响

采用口服汉防己甲素抗肝纤维化治疗，并以肝素乐保肝治疗作对照，观察治疗前后肝硬化患血清ＨＡ．ＰⅢＰ，肝组织学及肝肾功能的变化，随访１８个月。结果血清ＨＡ（４０４．８±１４０．０比５８８．０±１８１．２ｎｍｏｌ／Ｌ），ＰⅢＰ（１９．４±５．５比３１．８±１０．９ｎｍｏｌ／Ｌ）的含量及肝活组织免疫组化染色Ⅰ、Ⅲ型胶原与治疗关相比有显差异（Ｐ〈０．０５～０．０１），与对照组（ＨＡ５３９．８±１５０．２     

碳纤维微电极1．5次微分溶出伏安法测定雌激素的研究

本文报道了用碳纤维微电极测定雌激素的方法，找出了最佳条件，研究了电极反应机理，并对该法进行了实际应用。线性范围达９．０×１Ｏ－８～５．０×１０－６ｍｏｌ／Ｌ检出限５．０×１０－８ｍｏｌ／Ｌ。     

聚氯乙烯膜恩其明电极的研制

以恩其明－四苯硼钠高子缔合物为活性物质，研制了聚氯乙烯膜恩其明选择性电极，其能斯特响应范围和检测限分别为１．０×１０－２～１．０×１０－５ｍｏｌ／Ｌ，２．０×１０－６ｍｏｌ／Ｌ，对恩其明的回收率为９１％～１０４％，变异系数小于３％，此方法简便、快速、准确。     

地高辛的半微分阴极吸附伏安法研究

作者用半微分阴极吸附伏安法测定地高辛的方法，在１．０×１０＾－３ｍｏｌ／ＬＮａＯＨ底液中，于－１．０Ｖ富集于２ｍｉｎ，以１００ｍＶ／ｓ向阴极扫描，有一稳定的还原峰。在地高辛浓度为４．０×１０＾－７ｍｏｌ／Ｌ－１０．０×１０＾－７ｍｏｌ／Ｌ范围内，该还原峰峰高与地高辛浓度成线性，检出下限为１．０×１０＾－８ｍｏｌ／Ｌ。     

汉防己甲素抑制大鼠支气管肺泡灌洗液中SRS－A的活性

作者比较了大鼠在呼吸室内空气时及急性缺氧性肺动脉高压时支气管肺泡灌洗液（ＢＡＬＦ）中慢反应物质－Ａ（ＳＲＳ－Ａ）的生物活性，以及汉防己甲素对ＳＲＳ－Ａ活性的影响。结果表明，呼吸室内空气大鼠ＢＡＬＦ中未发现ＳＲＳ－Ａ活性；急性缺氧刺激使ＳＲＳ－Ａ活性明显增强；汉防己甲素（１０－５ｍｍｏｌ／Ｌ）可抑制ＳＲＳ－Ａ的活性。我们认为：大鼠急性缺氧性肺动脉高压的形成与肺内ＳＲＳ－Ａ合成增强有关；汉防己甲素降低缺氧性肺动脉高压作用的机理涉及时ＳＲＳ－Ａ活性的抑制。     

全固态麻黄碱电极的研制及应用

目的：制备一种新型的全固态麻黄电极，并对其性能和应用进行研究。方法：首先制备以脲醛树脂为框架的、ＫＣｌ粉末为活性物质的Ａｇ／ＡｇＣｌ固全参比电极，然后以此固体参比电极为基体、以含麻黄碱四苯硼酸离了对缔合物的ＰＶＣ膜为敏感元件，组装成全固态麻黄碱电极。结果：电极具有良好的稳定性，其Ｎｅｒｎｓｔｉａｎ线性响应范围为５×１０＾－４－１×１０＾－１ｍｏｌ／Ｌ斜率为５６．７ｍＶ／ｐＣ，检测下限为２．５×１０     

汉防己甲素对应激性溃疡防治作用的实验研究

目的 本研究采用拘束浸水应激法诱发大鼠的胃溃疡模型,以观察汉防己甲素对应激性溃疡的防治效果,并对其可能的作用机制作初步的探讨。方法 取６９只雄性ＳＤ大鼠随机分为６组;对照组（ｎ＝１６）汉防己甲素１组（ｎ＝１２）汉防己甲素２组（ｎ＝１）汉防己甲素３组（ｎ＝１０）阿托品组（ｎ＝１０）,西咪替丁组（ｎ＝１０）观察各组受试动物溃疡发生个数,溃疡指数等的变化;测定胃酸排出量,胃粘膜部丙二醛的含量,部分胃标本     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.