The Effect of Levamisole on Peripheral T-Lymphocytes of Patients with Malignant Lymphomas

The effect of various concentrations (0.015-10 μg/ml) of Levamisole (LMS) on the peripheral lymphocytes of patients with malignant lymphomas (ML) and normal donors was investigated in vitro. The parameters studied include : E rosettes forming cells (total T lymphocytes), active E rosettes (early T lymphocytes) and DNA synthesis induced by pitogens PHA and Con A.

LMS improved significantly lymphocyte response both in patients with ML and normal donors when the cells were stimulated by Con A. In both groups no significant effect was observed on the response to PHA nor on the percentage of E-rosettes, whereas the mean number of active E rosettes was significantly increased on all concentrations of the drug.

While in the normal subjects a positive statistical correlation between active E-rosettes and Con A response was observed, in patients with ML an inverse correlation was found. This latter correlation was partially reversed by LMS.     

Malignant Lymphomas in Japanese AIDS Patients

Seven non-Hodgkin's malignant lymphomas (MLs) were found in 25 Japanese AIDS (acquired immune deficiency syndrome) patients who died at two hospitals in Tokyo. All of these MLs originated from extranodal organs including the brain (three cases), skin (two cases) liver and adrenal gland. B cell markers were demonstrated in all of them. Epstein-Barr virus (EBV) capsid antigen (EBVCA) was demonstrated in 5/7, nuclear antigen (EBNA) in 2/2, the W fragment of EBV DNA by in situ hybridization in 5/7 and the same fragment by PCR in 6/7, indicating high association of these MLs with EBV. The adult T cell leukemia/lymphoma (ATL), endemic in south-west Japan and known to have a high association with HTLV-I, was not found in this series. The incidence of ML among the present AIDS cases is higher than in any other autopsy reports from western countries, although statistical analysis did not show this to be significant in comparison to some of these reports at a level of p<0.05. In spite of apparent higher incidence, the histopathologic and immunophenotypic characteristics of AIDS-associated MLs in Japan are in accordance with those in western countries. Acta Pathol Jpn 41: 744-750, 1991.     

Decreased CD8-p561ck Activity in Peripheral Blood T-Lymphocytes from Patients with Hereditary Haemochromatosis

Hereditary haemochromatosis (HH) is an autosomal recessive disease linked to certain MHC class-I specificities. The disease is characterized by increased iron absorption and, in some patients, abnormally low numbers of CDS8⁺ T cells in the periphery. We were interested in whether CD4- and CD8-associated p561ck kinase activities were altered in patients with HH. In a study of 18 patients with HH (with and without low numbers of CD8⁺ cells), the level of autophosphorylation of the CD8-associated p561ck as well as its phosphotransferase activity, as determined by phosphorylation of an exogenous substrate, was significantly reduced by two- to three-fold relative to a control population of 23 healthy blood donors (P < 6 × 10⁻⁷). CD8-p56 lck activity was decreased in 16 out of 18 patients (ranging from 1.5- to 10-fold decrease). By contrast, the level of CD4-p561ck activity did not show an overall decrease relative to controls. In addition to an occasional decrease in the amount of CD8-associated lck, HH patient-derived T cells showed a consistent decrease in the relative CD8-p561ck specific activity. Immunofiuorescence staining showed further that the difference could not be accounted by a discrepancy in the expression of CD8αα or CD8αβ complexes or MHC class I molecules. Decreased CD8-p561ck activity was seen both in patients undergoing intensive phlebotomy treatment and in patients in maintenance therapy (i. e. patients who had reached normal levels of iron stores), indicating that this abnormality does not appear to be corrected by iron depletion. To our knowledge, this is the first demonstration of an abnormality in a src-like receptor associated kinase in a human disease state linked to MHC class-I antigens.     

The Binding of Different Lectins on Peripheral Blood Mononuclear Cells from Patients with Chronic Inflammatory and Malignant Diseases

Peripheral blood mononuclear cells from patients with multiple myeloma, gastrointestinal tumors, and inflammatory bowel disease were analyzed for binding of various lectins. The results demonstrated that in most of the patients with multiple myeloma a significantly increased percentage of cells positive for Lotus tetragonolobus agglutinin (LTA), peanut agglutinin (PNA), soybean agglutinin (SBA), and wheat germ agglutinin (WGA), and a decreased number of Agaricus bisporus agglutinin (ABA) positive cells were present as compared to a normal control group. This could not be shown in malignant or inflammatory disorders of the gastrointestinal tract where only some patients exhibited an increased PNA and LTA binding, respectively. Patients with the systemic malignant disease differed from patients with solid localized tumors by a significantly altered number of ABA, LTA and SBA-positive peripheral blood mononuclear cells. Double fluorescence studies using monoclonal antibodies and lectins revealed that most of the cells expressing receptors for ABA had also receptors for OKT3, whereas most of the cells with receptors for LTA, PNA SBA, and WGA were found to be positive for OKM.     

The Effect of Levamisole on Gingival Inflammation in Man

The effects of levamisole on gingival inflammation and on cell-mediated immunity were examined in patients with gingivitis and in subjects with clinically healthy gingiva. Treatment with levamisole promoted the development of gingivitis and aggravated the severity of established gingivitis, although the amount of bacterial plaque at the gingival margin was not significantly increased. This effect was short-lasting, as the gingival indices returned almost to base-line values by the end of the fifth month after treatment with levamisole. The development of gingivitis was associated with increased in vitro lymphocyte stimulation by antigens from oral bacteria. The role of cell-mediated immunity in the pathogenesis of gingival inflammation is discussed.     

The Effect of Levamisole in Experimental Murine Amyloidosis

Abstract

In casein-induced murine amyloidosis various lines of investigation have implicated immunodeficiency as playing a role in amyloid formation. In this study, the immuno-potentiating agent levamisole failed to prevent amyloido-genesis or to accelerate resolution of preformed amyloid deposits in the mouse model. The serum precursor of amyloid, serum amyloid protein A (SAA), was increased by levamisole in both normal and amyloidotic mice.     

胺碘酮治疗器质性心脏病并恶性室性心律失常的疗效观察

目的 观察胺碘酮治疗器质性心脏病并恶性室性心律失常的近期疗效。方法 对收入CCU符合入选条件的68例器质性心脏病并恶性室性心律失常患者随机分为2组。治疗组36例：部分病例采用胺碘酮转复；其余采用电转复，成功后用胺碘酮维持窦性心律（窦律）。对照组32例：应用利多卡因转复及维持窦律，将两组转复及维持窦律结果进行比较。结果 治疗组总有效率77．8％（28／36），明显高于对照组56．25％（18／32），P〈0．05，部分利多卡因无效患者用胺碘酮仍有效。结论 胺碘酮是治疗恶性室性心律失常最有效药物之一，其临床疗效优于利多卡因。     

The Effect of Levamisole in Experimental Murine Amyloidosis

In casein-induced murine amyloidosis various lines of investigation have implicated immunodeficiency as playing a role in amyloid formation. In this study, the immuno-potentiating agent levamisole failed to prevent amyloido-genesis or to accelerate resolution of preformed amyloid deposits in the mouse model. The serum precursor of amyloid, serum amyloid protein A (SAA), was increased by levamisole in both normal and amyloidotic mice.     

针对性围手术期护理对甲状腺恶性肿瘤合并 糖尿病患者的效果观察

目的 观察针对性围手术期护理模式应用于甲状腺恶性肿瘤合并糖尿病患者的临床效果。方法 将2015年9月~2017年9月在我院进行治疗的90例甲状腺恶性肿瘤合并糖尿病患者通过单双号分组法分为常规组和实验组。常规组实施传统护理模式,实验组采用针对性围手术期护理模式。对比两组患者护理前后的血糖水平、满意度以及住院天数。结果 实验组患者干预后空腹血糖水平为（8.15±0.67）mmol/L,低于常规组（11.67±0.64）mmol/L,差异有统计学意义（P＜0.05）;实验组护理满意度为95.56%,高于常规组的80.00%,差异有统计学意义（P＜0.05）。结论 应用针对性围手术期护理模式可以较好地控制甲状腺恶性肿瘤合并糖尿病患者的血糖,提高患者的满意度,促进患者康复。     

The Effect of Interferon on Activated Peripheral Blood t Lymphocytes in Patients with Chronic Active Hepatitis B

The effect of interferon-alpha2b treatment on activated T lymphocytes -T cells expressing interleukin-2 receptors -(IL-2R) was studied in IS patients with chronic active hepatitis B. Blood samples were taken before, on the 2nd, 4th and 5th month of treatment. Patients were divided in 3 groups according to their response to therapy(complete, partial, no response). At the end of treatment, IL-2R+ cells were decreased in the group of patients with complete response, unchanged in patients with partial response and increased in patients with no response. These results confirm the immunomodulatory effect of interferon and reflect the effect of treatment in the management of the disease.     

State of Central and Peripheral Pools of Hemopoietic Precursor Cells in Patients with Malignant Neoplasms during Chemotherapy

We studied toxic effects of various schemes of cytostatic treatment on central and peripheral pools of hemopoietic precursor cells in patients with III–IV stages of lung cancer and breast cancer. It was found that erythro- and granulomonocytopoietic precursors are characterized by high resistance to cytostatic treatment compared to morphologically discernible bone marrow elements, which is probably a evolutionary developed property of this cell type aimed at rapid recovery of the hemopoietic tissue. __________ Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 234–238, November, 2005     

缬沙坦对原发性高血压患者外周PBMC中IL-17表达的影响

目的 探讨血管紧张素Ⅱ受体阻滞剂缬沙坦对高血压患者外周血单核细胞中IL-17表达的影响。方法 选取2016年9月~2017年10月在我院收治的原发性高血压患者98例,将所有患者按照随机数字表法分为缬沙坦组和硝苯地平组,各49例,缬沙坦组患者使用缬沙坦治疗,硝苯地平组患者使用硝苯地平缓释片治疗,另选取健康体检者20例为正常组。采集患者治疗前及治疗4周后静脉血,分离PBMC体外培养5 h后用RT-PCR法测定IL-17 mRNA表达水平;培养上清液采用ELISA法测定IL-17浓度。结果 治疗4周后缬沙坦组与硝苯地平组血压变化比较,差异无统计学意义（P＞0.05）;缬沙坦组治疗后PBMC中IL-17 mRNA表达及培养上清液中IL-17浓度分别为（0.29±0.09）,（76.16±20.56）pg/ml,低于硝苯地平组的（0.50±0.15）,（137.84±39.97)pg/ml,组间比较,差异有统计学意义（P＜0.05）。结论 缬沙坦可显著降低高血压患者PBMC中IL-17的表达,有助于延缓疾病发展。     

Expression of Vimentin and Epithelial Membrane Antigen in Human Malignant Lymphomas

Immunoreactivity with monoclonal antibodies against the intermediate filament protein, vimentin, and epithelial membrane antigen (EMA) was examined in 330 cases of lymphoma (317 non-Hodgkin's and 13 Hodgkin's lymphomas), 12 reactive lymph nodes and mononuclear cells of the peripheral blood using either indirect immunoperox-idase staining or the avidin-biotin immunoperoxidase complex technique. The cell origin of each tumor was established using a panel of monoclonal antibodies against lymphocyte differentiation antigens. There were 41 T cell, 247 B cell and 29 undetermined lymphomas, and 13 cases of Hodgkin's disease in the series. Vimentin was expressed in 24 T-cell lymphomas (58.5%) and 60 B cell lymphomas (24.2%). This difference in frequency was statistically significant. Vimentin expression in follicular lymphomas was less frequent than in diffuse B-cell lymphomas. In diffuse lymphomas, small and medium cell types were more reactive with anti-vimentin than large cell types. Reed-Sternberg cells (R-S cells) in Hodgkin's disease were positive for vimentin in 11 cases (84.6%). The frequency of EMA reactivity in lymphomas was low, particularly in T cell lymphomas. No positive cases were found among follicular lymphomas. In diffuse non Hodgkin's lymphomas, EMA was expressed only in mixed and large cell types, but never in smaller ones. In conclusion, monoclonal antibodies against vimentin and EMA appear to be of limited usefulness for the diagnosis of non Hodgkin's lymphomas, but anti vimentin antibody may be used as an adjunct to the diagnosis of R-S cells in Hodgkin's disease.     

Interleukin-2 Induced Mitogenesis of Human Peripheral Blood T-Lymphocytes: Role of Accessory Cells

We and others have shown that Interleukin-2 (IL-2) is mitogenic to a subset of unstimulated T lymphocytes in human peripheral blood (1-7). We extend our work here in showing that prolonged continuous exposure of human peripheral blood lymphocytes to exogenous IL-2 throughout the 7-8 day culture is not necessary since mitogenesis occurs reproducibly after short term (2-3 hr) pulse exposure. The mitogenic effect of pulse exposure to IL-2 is not significantly reduced by inclusion of anti-Tac monoclonal antibody in the pulsing medium. However, anti-Tac monoclonal antibody markedly inhibits the response if present continuously throughout the 7 day culture.

The mitogenic effect of IL-2 is dependent on the presence of accessory cells (monocytes) but the accessory cell requirement can be replaced by the phorbol ester TPA (10^-8 to 10^-11 M). Purified monocytes subjected to short term pulse exposure to IL-2 can cause proliferative response in unprimed autologous lymphocytes in cocultures. The mitogenic effect of IL-2 pulsed monocytes can not be suppressed by inclusion of anti-Tac antibody in the pulsing medium although the same concentration of the antibody suppresses the effect if present throughout culture. The response of lymphocytes to IL-2 pulsed monocytes is not inhabitable by the continuous presence of a monoclonal antibody to human HLA-DR antigens (OK-Ial) in culture.     

Interleukin-2 Induced Mitogenesis of Human Peripheral Blood T-Lymphocytes: Role of Accessory Cells

We and others have shown that Interleukin-2 (IL-2) is mitogenic to a subset of unstimulated T lymphocytes in human peripheral blood (1–7). We extend our work here in showing that prolonged continuous exposure of human peripheral blood lymphocytes to exogenous IL-2 throughout the 7–8 day culture is not necessary since mitogenesis occurs reproducibly after short term (2–3 hr) pulse exposure. The mitogenic effect of pulse exposure to IL-2 is not significantly reduced by inclusion of anti-Tac monoclonal antibody in the pulsing medium. However, anti-Tac monoclonal antibody markedly inhibits the response if present continuously throughout the 7 day culture.

The mitogenic effect of IL-2 is dependent on the presence of accessory cells (monocytes) but the accessory cell requirement can be replaced by the phorbol ester TPA (10^?8 to 10^?11 M). Purified monocytes subjected to short term pulse exposure to IL-2 can cause proliferative response in unprimed autologous lymphocytes in cocultures. The mitogenic effect of IL-2 pulsed monocytes can not be suppressed by inclusion of anti-Tac antibody in the pulsing medium although the same concentration of the antibody suppresses the effect if present throughout culture. The response of lymphocytes to IL-2 pulsed monocytes is not inhabitable by the continuous presence of a monoclonal antibody to human HLA-DR antigens (OK-Ial) in culture.     

Peripheral T-cell Lymphomas Immunologic and Enzyme Histochemical Analysis of 15 Cases

Ffteen cases of peripheral T cell lymphoma were studied to evaluate the respective properties of various histologic types using enzyme histochemical and ultrastructural examinations in addition to immunological methods. Eleven cases in an ATLA negative group manifested various histologic patterns such as IBL like, pleomorphic and Lennert's lymphomas in comparison with the relatively monomorphic proliferation of neoplastic lymphoid cells in the 4 ATLA positive cases. The presence of neoplastic clear cells is characteristic of peripheral T-cell malignancies, and is likely to be found in CD4 lymphomas. There is an occasional reaction of epithelioid histiocytes and plasma cells with eosinophils, the former being designated Lennert's lymphoma and the latter IBL like T-cell lymphoma. Immunological examination revealed four immunophenotypic patterns: (1) CD2⁺3⁺4⁺8⁺, (2) CD2⁺ 3⁻4⁺8⁻, (3) CD2⁺3⁺4⁻8⁺, and (4) CD2⁺3⁺4⁺8⁺, but did not provide information concerning the intimate relationship between histologic types and immuno phenotyes. β-Glucuronidase reactivity, however, contributed to the distinction between helper and suppressor T cell malignancies, suggesting its usefulness for distinguishing these two cell types and their malignant counterparts.     

普伐他汀对ACS患者外周血炎症细胞因子表达的影响

目的:探讨普伐他汀治疗急性冠状动脉综合征(ACS)的可能作用机制。方法:将ACS患者80例分为治疗组和对照组,每组均为40例。对照组予以常规治疗,治疗组在上述治疗基础上给予口服普伐他汀20mg,1次/d,两组均治疗6个月为1个疗程。结果:两组患者治疗前血浆MMP-9和IL-18含量无明显差异(P>0.05)。治疗1个疗程后,两组患者血浆MMP-9和IL-18水平均较治疗前明显下降(P<0.05或P<0.01),但治疗组下降更加明显(P<0.05)。两组患者治疗前颈动脉彩超检查,颈动脉斑块大小、厚度、数量和颈总动脉中层内膜厚度(IMT)比较基本相似(P>0.05)。治疗1个疗程后,治疗组颈动脉斑块大小、厚度、数量和IMT均较治疗前明显减小(P<0.05),而对照组变化不明显(P>0.05),两组比较差异明显(P<0.05)。结论:普伐他汀对ACS患者外周血MMP-9和IL-18的表达有一定调节作用,从而改善动脉粥样硬化情况影响患者预后。     

卡介菌多糖核酸对寻常型白癜风患者外周血Treg细胞的影响

目的 探讨卡介菌多糖核酸BCG-PSN联合308 nm准分子光对寻常型白癜风患者外周血Treg细胞的影响。方法 选取2013年9月~2017年5月我院收治的寻常型白癜风患者120例设为治疗组,采用随机数字表法分成Ⅰ组和Ⅱ组,各60例。Ⅰ组采用308 nm准分子光单独治疗,Ⅱ组联合卡介菌多糖核酸肌注。另选取同期我院健康体检者30例作为对照组,比较治疗组与对照组外周血Treg细胞比值、治疗组不同分期外周血Treg细胞比值、各组治疗前后外周血Treg细胞比值以及Ⅰ组和Ⅱ组治疗总有效率。结果 治疗组外周血Treg细胞比值低于对照组,差异有统计学意义（P＜0.05）;进展期外周血Treg细胞比值低于稳定期,差异有统计学意义（P＜0.05）;与对照组比较,Treg细胞比值均降低,且进展期下降更明显;治疗后Ⅰ组、Ⅱ组Treg细胞比值均较治疗前升高,且Ⅱ组升高更为明显,差异有统计学意义（P＜0.05）;治疗后Ⅰ组Treg细胞比值低于对照组,差异有统计学意义（P＜0.05）;Ⅱ组治疗总有效率高于Ⅰ组,差异有统计学意义（P＜0.05）。结论 BCG-PSN可以通过增加Treg细胞水平提高患者细胞免疫功能,临床疗效确切,安全性高,不良反应少,值得临床应用。